DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) An Efficient Method for Neuron-Like Differentiation of SH-SY5Y Neuroblastoma Cells Using Retinoic Acid and Laminin-Rich Extracellular MatrixGenesisAugust 2, 2026Marina Mantellatto Grigoli, Bianca Cruz Pachane, Angelina Maria Fuzer, Sabrina Dorta de Oliveira, Ana Beatriz Aparecida Targas, Vanessa Alexandre-Silva, Heloisa Sobreiro Selistre-de-Araujo, Patricia Regina Manzine, Marcia Regina Cominetticounterstained with phalloidin‐A488 (Molecular Probes Cat# A12379) and DAPI (0.5 mg/mL, Abcam Cat# ab228549) before slide assembly using the Fluoromount G mounting media (EM Sciences Cat# 17984‐25). Cells were

(2) Focal astrocyte loss reveals nuclear translocation during lesion repopulationNat NeurosciJuly 23, 2026Marina Herwerth, Matthias T. Wyss, Nicola B. Schmid, Anna Lasne, Jacqueline Condrau, Luca Ravotto, José María Mateos Melero, Andres Kaech, Gustav Bredell, Carolina Thomas, et al.stainings, slices were incubated with the appropriate secondary antibodies and DAPI (1:1,000; Abcam, ab228549) for nuclear counterstaining. Confocal microscopy Samples were scanned with Zeiss confocal microscopes

(3) Connexin 50 mediates disease-relevant alpha-synuclein oligomer propagation and neuroinflammation in neurodegenerative diseaseiScienceJuly 17, 2026Nicha Puangmalai, Urmi Sengupta, Nemil Bhatt, Nopparat Suthprasertporn, Fadhl Al-Shaebi, Nikita Shchankin, Jia Yi Liew, Twisha Chatterjee, Dhvani Pansuriya, Ahmet E. Balci, et al.receptor blocker (BD Bioscience, 553141). For cell population gating, DAPI staining solution (Abcam, ab228549) for live-dead cell gating and immunolabeling with APC-labeled ACSA-2 antibody (Miltenyi Biotec, 130-117-535)

(4) Mitochondrial-generated G-quadruplex-forming lncRNAs regulate heme homeostasisiScienceJuly 17, 2026Vinodh J. Sahayasheela, Ryohei Noizumi, Manendra B. Lankadasari, Takumi Terada, Mitsuharu Ooga, Atharv Kulkarni, Shinjiro Suzuki, Takuya Hidaka, Li Cai, Ganesh N. Pandian, et al.,Thermo Fisher Scientific,Cat# M22426 Hoechst-33342,Thermo Fisher Scientific,Cat# H3570 DAPI Staining Solution,Abcam,Cat# ab228549 ProLong Gold Antifade Mountant,Thermo Fisher Scientific,Cat# P36930 PolyJet transfection reagent,SignaGen,Cat#

(5) A peptide-based screen for cell death inhibitors identifies the cytoprotective compound CDL36bioRxivJuly 10, 2026Zintis Inde, Sebastian Keppler, Jesse Gelles, Cameron S. Fraser, Adam Presser, Jarvier N. Mohammed, May Jung, David S. Garvey, Tudor Moldoveanu, Jerry E. Chipuk, et al.cytochrome c antibody (Biolegend, cat #612310) diluted 1:1000 and DAPI staining solution (Abcam, cat #ab228549) diluted 1:100. 10 μL/well staining solution was added and incubated to stain for 24 hours at 4 °C with

(6) Compound 4′-O-methylbroussochalcone B attenuates LPS/ATP-induced macrophage pyroptosis in vitro through modulation of the NLRP3 inflammasome and MAPK/NF-κB pathwaysFrontiers in PharmacologyJune 24, 2026Jihee Choi, You Chul Chung, Ami Lee, Han Na Kang, Youn-Hwan Hwangwere incubated with propidium iodide (PI; P3566) for 5 min and counterstained with Hoechst 33,258 (ab228549, Abcam). Fluorescence images were captured using an ImageXpress Micro 4 High-Content Screening System

(7) Targeting the 4EBP1/HSP90β/Nrf2 Axis Sensitizes β-catenin-mutant Hepatocellular Carcinoma to mTOR Inhibitors via Ferroptosis InductionJ Clin Transl HepatolJune 16, 2026Rong Li, Yi Zhou, Zimu Wang, Gang Liu, Deyu Fan, Lanxuan Huang, Fule Deng, Ning Wei, Runze Shang, Meng Xufluorescence-labeled secondary antibodies at room temperature for 2 h. Nuclei were stained with DAPI (ab228549, Abcam). Finally, images were captured using an LSM880 confocal microscope (Leica, Germany). GSH/GSSG

(8) Hypoxia‐preconditioned adipose‐derived mesenchymal stem cells‐derived exosomes transferring H19 obstruct neutrophil extracellular traps formation via HOXA5 ‐mediated inactivation of TLR4 / NF ‐ κB / NLRP3 inflammatory signalingJournal of Diabetes InvestigationJune 8, 2026Li Qian, Xianxi Meng, Bairong Fang, Li PiAbcam) for 2 h and then incubated with the 4′,6‐diamidino‐2‐phenylindole (DAPI) staining solution (#ab228549, Abcam) for 5 min. Fluorescence images (at ×400 magnification) were observed under a SP8 LIGHTNING confocal

(9) Neuregulin-1 promotes early regenerative and autophagic responses after ischemic stroke via spatial proteomicsFront Cell NeurosciJune 2, 2026Jessica M. Noll, Christopher McGinley, Catherine J. Augello, Esra Kürüm, Liuliu Pan, Anna Pavenko, Andy Nam, Byron D. Ford, Gregory D. Fordfor 2 min. Sections were then incubated in a freshly prepared solution of 0.0004% FJB with DAPI (Cat# ab228549, Abcam, Waltham, MA) for 20 min, rinsed in distilled water 3 times for 2 min, and then dried at 50°C. For

(10) A Human Vocal Fold Organ-On-Chip for Studying Platform-Dependent Mucosal Responses to Particulate MatterbioRxivJune 2, 2026Patrick T. Coburn, Meghana Munipalle, Yin Liu, Vlasta Lungova, Samjhana Thapa, Lisa Martignetti, Xinyu Liu, Gilles Maussion, Carol X.-Q. Chen, Thomas M. Durcan, et al.anti-chicken (1:1000, ThermoFisher A-21103)",Intermediate filaments DAPI,Nuclear DNA,—,1:5000,Abcam ab228549,—,—,Counter-staining TaqMan assays for quantitative real-time PCR,TaqMan assays for quantitative real-time PCR

(11) Herkinorin Exerted Neuroprotective Effects After Spinal Cord Injury by Suppression of NF-κB Pathway ActivationNeurochem ResJune 1, 2026Jianyong Wang, Jiacheng Yu, Wen Han, Chaochao Ding, HaiFeng Wang, Siqi Chen, Yi Huang, Wenting Lan(Goat anti-Rabbit IgG H&L (Alexa Fluor®488), Abcam, ab150077; Goat anti-Mouse IgG H&L (Alexa Fluor®488), Abcam, ab150113) and DAPI (Abcam, ab228549). Images were acquired using a fluorescence microscope (TCS SP8, Lecia) Six random fields from three

(12) Six-month evaluation of normal mouse brain side effects: Comparing FLASH and conventional proton partial brain irradiationClin Transl Radiat OncolMay 9, 2026Manuel Bernabei, Sindi Nexhipi, Elisabeth Bodenstein, Felix Horst, Armin Lühr, Jörg Pawelke, Moritz Schneider, Michael Schürer, Roman Schwarz, Antje Dietrich, et al.studying microglial morphology. All slides were counterstained with DAPI (4′,6-diamidino-2-phenylin-dole, ab228549, Abcam, Cambridge, UK) for nuclei detection. Whole slide imaging was performed using the AxioScan.Z1

(13) Imaging mass cytometry of the immune microenvironment in alveolar echinococcosisFront Cell Infect MicrobiolMay 8, 2026Yanyan Ma, Yaogang Zhang, Tao Zhang, LingQing Zhang, Zhixin Wang, Zihan Yang, Xinjian Guo, Jingqi Han, Jing Hou, Meiyuan Tian, et al.(Goat Anti-Rabbit IgG H&L, Abcam, ab205718, 1:20000). The nuclei were stained with DAPI Staining Solution (Abcam, ab228549), antifade mounting medium was added, and the slides were sealed. Images were acquired using a TG TissueFAXS

(14) Phaeochromocytomas and paragangliomas harbour tumour-initiating SOX2+ stem cellsEndocr Relat CancerApril 22, 2026Yasmine Kemkem, Mark Quinn, Bence Kövér, Alice Santambrogio, James Kaufman-Cook, Olivia Sherwin, Laura D Scriba, Dimitria Brempou, Miriam Vazquez Segoviano, Dylan Cameron, et al.fluorophore-conjugated antibodies (Goat anti-mouse594 (CAT#ab150116, Abcam, UK, 1:500) and DAPI (CAT# ab228549, Abcam, UK, 1:5,000) in 1% donkey serum/blocking buffer. Cryosections were air-dried and washed in PBS.

(15) PFKFB4 promotes M2 polarization of tumor-associated macrophages through aerobic glycolysis-mediated modification of histone H3K18 lactylation in hepatocellular carcinomaCancer & MetabolismApril 15, 2026Chao Gan, Dan Wu, Yue Yuan, Lingling Li, Jia Bai, Yanmei Xu, Haihong Lvlight for 1 h. Cell nuclei (blue) were stained with 4’,6-diamidino-2-phenylindole staining solution (ab228549, Abcam). Sections were sealed using an anti-fluorescence quenching sealing agent (A598329, Aladdin Biochemical

(16) Programmable Lipid Functionalization of Nucleic Acid Nanoparticles Modulates Liver Cell-Type TargetingACS Applied Materials & InterfacesApril 8, 2026Hyun Min Kim, Marjan Omer, Grant A. Knappe, Patrick McMullen, Duy An Le, Ashwin Pasupathy, Daniel G. Anderson, Mark BatheE (#MAB41444-SP) were purchased from R&D Systems. Pitstop 2 (ab120687) and DAPI staining solution (ab228549) were purchased from Abcam. Twelve well chamber removable microscopy slides (#81201) were purchased

(17) TGFβ1-loaded extracellular matrix hydrogels promote skeletal muscle stem cell regeneration via m6A-mediated integrin–ERK signalingJournal of Translational MedicineApril 6, 2026Menghai Zhu, Peng Zou, Gang Chen, Chong Lian, Benggang Qingoat anti-rabbit IgG H&L (Alexa Fluor® 488) (1:1000 dilution, ab150077, Abcam) were used. Cell nuclei were counterstained with DAPI (ab228549, Abcam, USA). Cell images were obtained by an AxioVision 4.8 camera and an Axio Observer Z1 inverted

(18) TNF inhibits SARS-CoV-2 induced cell-cell fusion through activating the SDC4-RhoA signaling to promote actin bundles formationCell InsightApril 1, 2026Dong Duan, Xu Zheng, Yanqiu Zhou, Mengmeng Cui, Yunyi Li, Xiaoxian Cui, Yuying Yang, Min Chen, Huanyu Wu, Xin Chen, et al.(Invitrogen, A-21424, 1: 400) and goat anti-rabbit IgG-647 (Invitrogen, A-21236, 1: 400), DAPI (Abcam, ab228549, 1:2 000) and antifade mounting medium (vector labs, H-1400-10). Pam3CSK4 (Invivogen, tlrl-pms), TPCA1

(19) Sacubitril/Valsartan Ameliorates Inflammation and Oxidative Stress in Hypertensive Heart Disease by Upregulating CAMKK2 Protein and Modulating the AMPK / AKT / GSK ‐3β AxisThe Kaohsiung Journal of Medical SciencesApril 1, 2026Yan‐Jun Yang, Jiu‐Sheng Lifluorescein, while total nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole staining solution (DAPI, ab228549, Abcam). The apoptosis rate was determined by the ratio of TUNEL‐positive to DAPI‐stained nuclei. For

(20) Anti-Cancer Effect of a New 5-FU Derivative Containing Triazole-Bearing Mannose (5-FUD-MAN) Against Human Breast Cancer Cells Through LC3B-Mediated Cell DeathJ Biochem Mol ToxicolMarch 29, 2026Ebru Şanci, Azada Aliyeva, Buket Bakan, Mustafa Özkaraca, Erkan Halay, Kadir Ay, Tamer Karayildirim, N. Ulku Karabay YavasogluCells were then incubated for 1 h at room temperature in the dark. Finally, DAPI (Abcam, Catalog no. ab228549) was applied to the washed cells and examination was performed by using fluorescence microscopy (Zeiss

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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