DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(261) Clemastine improves electrophysiologic and histomorphometric changes through promoting myelin repair in a murine model of compression neuropathyScientific reportsOctober 22, 2021Lee JI, Park JW, Lee KJ, Lee DH.antibody (1:200, ab31851; Abcam) in 5% BSA. Incubation with 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, ab228549; Abcam) and fluorescent secondary antibodies (Alexa Fluor 488, and 594-conjugated antibodies [1:500,

(262) Treatment With Melatonin After Corneal Graft Attenuates RejectionFrontiers in PharmacologyOctober 19, 2021Ziqian Zhu, Ruiping Peng, Hongyi Shen, Lei Zhong, Siqi Song, Tao Wang, Shiqi Ling(Cell signaling Technology, Germany, 1:1,000) for 1 h at room temperature, followed by DAPI (Abcam, Ab228549) solution staining. Photographs were taken with a fluorescence microscope (DM 4000B). The number of

(263) LGR5-Positive Supporting Cells Survive Ototoxic Trauma in the Adult Mouse CochleaFrontiers in Molecular NeuroscienceOctober 5, 2021Natalia Smith-Cortinez, Rana Yadak, Ferry G. J. Hendriksen, Eefje Sanders, Dyan Ramekers, Robert J. Stokroos, Huib Versnel, Louise V. StraatmanInvitrogen, A-21207), donkey-anti Goat-Alexa 488 (1/200, Abcam, AB150129), and DAPI solution (1/500, Abcam, AB228549) for 90 min at room temperature. Lastly, specimens were washed in PBS and mounted in Vectashield Antifade

(264) The molecular mechanism underlying mitophagy-mediated hippocampal neuron apoptosis in diabetes-related depressionJournal of cellular and molecular medicineAugust 1, 2021Liu J, Liu L, Han YS, Yi J, Guo C, Zhao HQ, Ling J, Wang YH.paraformaldehyde for 30 minutes and then incubated with 4′,6‐diamidino‐2‐phenylindole (DAPI) (1:800, ab228549, Abcam, USA) at room temperature for 15 minutes. Autophagosome, autolysosome and autophagic flux were

(265) Lamina-specific properties of spinal astrocytesGliaJuly 1, 2021Kronschläger MT, Siegert ASM, Resch FJ, Rajendran PS, Khakh BS, Sandkühler J.always covered with foil. Afterwards, slices were incubated in a DAPI‐staining solution (20 μM; Abcam ab228549) for 2 hr on a shaker. Slices were washed ×3 in 1× PBS for 15 min before mounting on glass sides using

(266) Photodynamic Therapy for Benign Cutaneous Neurofibromas Using Aminolevulinic Acid Topical Application and 633 nm Red Light IlluminationPhotobiomodulation, Photomedicine, and Laser SurgeryJune 1, 2021Brendan Quirk, Edit Olasz, Suresh Kumar, Donald Basel, Harry Whelan(DeadEnd Colorimetric TUNEL System, Promega, Madison, WI), and DAPI (4′,6-diamidino-2-phenylindole) staining (ab228549; Abcam, Cambridge, MA). Additional follow-up visits for measurement of lesions were made at periods

(267) Recombinant human bone morphogenetic protein-4 enhances tendon-to-bone attachment healing in a murine model of rotator cuff tearAnnals of Translational MedicineMay 14, 2021Huabin Chen, Zhanwen Wang, Li Zhou, Bing Wu, Hongbin Lu, Ciliu Zhang, Tao ZhangUK) at room temperature for 1 hour, and finally incubated with 4’,-diamidino-2-phenylindole (DAPI) (ab228549, Abcam). All images were observed and captured using a Zeiss Axio Imager (Zeiss, Solms, Germany). According

(268) High Glucose Attenuates Cardioprotective Effects of Glucagon-Like Peptide-1 Through Induction of Mitochondria Dysfunction via Inhibition of β-Arrestin-SignalingFrontiers in PhysiologyMay 13, 2021Xietian Pan, Chengxiang Li, Haokao GaoUnited States 15596018 MEBSTAIN Apoptosis TUNEL kit Takara, Japan MK500 DAPI Abcam, United Kingdom ab228549 Caspase-3 assay kit Clontech, CA 630217 ROS assay kit Abcam, United Kingdom ab113851 CCK-8 assay

(269) Uridine Diphosphate Promotes Rheumatoid Arthritis Through P2Y6 ActivationFrontiers in PharmacologyApril 19, 2021Hongxing Wang, Hui Wu, Kehua Fang, Xiaotian Changab150078) for 1 h in the dark. Nuclei were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI) (Abcam, ab228549). Images were acquired under a fluorescence microscope, and quantification of the signal density was

(270) Abnormal Glu/mGluR_2/3/PI3K pathway in the hippocampal neurovascular unit leads to diabetes-related depressionNeural regeneration researchApril 1, 2021Liu J, Han YS, Liu L, Tang L, Yang H, Meng P, Zhao HQ, Wang YH.SA00003-2; Proteintech) at 37°C for 30 minutes, and stained with 4′,6-diamidino-2-phenylindole (1:800; Cat# ab228549; Abcam) at room temperature for 15 minutes. A high-content analysis system was used to analyze protein expression

(271) Lipid Rafts Interaction of the ARID3A Transcription Factor with EZRIN and G-Actin Regulates B-Cell Receptor SignalingDiseases (Basel, Switzerland)March 20, 2021Schmidt C, Christian L, Smith TA, Tidwell J, Kim D, Tucker HO.goat anti-mouse IgG H&L (Alexa Fluor® 488; ab150117, Abcam), and stained with DAPI (Staining Solution ab228549, Abcam). Visualization is described below and shown in Figure 2B and Figure S4. Diseases 2021, 9,

(272) Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer CellsBiomedicinesMarch 13, 2021Kang DY, Sp N, Lee JM, Jang KJ.Morphological Analysis Chromatin condensation during apoptosis was examined with DAPI staining solution (ab228549, Abcam, Cambridge, MA, USA). A549 and H460 cells were seeded in 6-well plates at a density of 1.5 ×

(273) Surfactant location and internal phase volume fraction dictate emulsion electrospun fiber morphology and modulate drug release and cell responseBiomaterials ScienceFebruary 23, 2021Pamela M. Johnson, Kelsey E. Knewtson, Jacob G. Hodge, Justin M. Lehtinen, Anna S. Trofimoff, D. Joseph Fritz, Jennifer L. Robinsonwith 300 µL PBS. Nuclei were stained for 15 minutes at room temperature RT using DAPI (100 µM; Abcam, ab228549). Meshes were washed 2x with 300 µL of PBS before being imaged on a Zeiss Axio Observer A1 Inverted

(274) Astragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytesMolecular Biology ReportsFebruary 19, 2021Yingjun Zang, Shuang Liu, Aili Cao, Xiangyu Shan, Wenjuan Deng, Zhijun Li, Hao Wang, Yunman Wang, Li Wang, Wen PengMolecular Probes). The mitochondria were labeled with Mito tracker and the nuclei were visualized by DAPI (ab228549, Abcam, Cambridge, MA, USA) staining. Images were taken using LEICA laser scan microscope. Apoptosis assay

(275) Neonatal AAV gene therapy rescues hearing in a mouse model of SYNE4 deafnessEMBO Molecular MedicineFebruary 5, 2021Shahar Taiber, Roie Cohen, Ofer Yizhar‐Barnea, David Sprinzak, Jeffrey R Holt, Karen B Avrahammyosin VIIa (Proteus Biosciences 25‐6790) 1:250, mouse anti‐FLAG (Sigma F3165) 1:1,000, DAPI (Abcam ab228549) 1:1,000, goat anti‐mouse (Abcam ab150119) 1:250, and goat anti‐rabbit Alexa Fluor 488 (Cell Signaling

(276) Isolation and Characterization of Primary Retinal Microglia From the Human Post-mortem Eyes for Future Studies of Ocular DiseasesFrontiers in cellular neuroscienceJanuary 1, 2021Wang L, Qian Y, Che X, Jiang J, Suo J, Wang Z.1:1,000; Alexa Fluor 647 of goat anti-rabbit, Yeasen, 1:1,000) at room temperature for 1 h. DAPI (Abcam, #ab228549, 1:1,000) was used for staining nuclear DNA. The morphology of retinal microglia and their specific

(277) NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of EndometriosisFrontiers in immunologyJanuary 1, 2021Guo X, Xu X, Li T, Yu Q, Wang J, Chen Y, Ding S, Zhu L, Zou G, Zhang X.Alexa Fluor 647 donkey anti-rabbit, 1:500, ab150075, Abcam) and 4′,6-diamidino-2-phénylindole (DAPI, ab228549, Abcam) were then used for detection. Images were acquired using an Olympus FV1200 confocal microscope

(278) TFF-1 Functions to Suppress Multiple Phenotypes Associated with Lung Cancer ProgressionOncoTargets and therapyJanuary 1, 2021Minegishi K, Dobashi Y, Tsubochi H, Hagiwara K, Ishibashi Y, Nomura S, Nakamura R, Ohmoto Y, Endo S.Cambridge, UK) IgG (1:1000) for 1h at RT. Samples were stained with 4′,6-diamidino-2-phenylindole (DAPI, ab228549, Abcam) and the fluorescence images were digitally captured on an All-in-One fluorescence microscope

(279) Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526bPeerJJanuary 1, 2021Zhang Y, Liu S, Ding L, Wang D, Li Q, Li D.the dark for 30 min at room temperature. Cell nucleus was stained with DAPI staining solution (10 mM, ab228549; Abcam) for 5 min. For quantification of apoptosis, the cells were observed by a confocal microscope

(280) The subcellular redistribution of NLRC5 promotes angiogenesis via interacting with STAT3 in endothelial cellsTheranosticsJanuary 1, 2021Xu X, Shi Y, Luan P, Kou W, Li B, Zhai M, You S, Yu Q, Zhuang J, Jian W, et al.sectioning at 8 mm thickness. Sections were incubated in primary antibodies (Table S1.1). DAPI 1:5000 (Cat# ab228549, Abcam) was used as a nuclear counterstain. Tissue was visualized using objective on Nikon Eclipse TE-2000U

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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