Human HGF Quantikine ELISA Kit DHG00B from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman HGF Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDHG00B
NCBI Full Gene Namehepatocyte growth factor
NCBI Gene AliasesDFNB39, F-TCF, HGFB, HPTA, SF
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
Assay Time4.0 hours
Detection TargetHGF
Sensitivity24.1 pg/mL
Sample TypeCell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
ReactivityHuman
Detection Range156.00 - 10000 pg/mL
ApplicationsELISA
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Rejuvenation of mesenchymal stromal cells via partial reprogramming enables scalable generation of transcriptionally diverse MSC librariesStem Cell Research & TherapyMarch 20, 2026Haochen Tu, Aoi Hosaka, Genki Hichiwa, Yayan Wang, Kanako Kazuki, Toshiaki Tabata, Mitsuhiko Osaki, Yuji Nakayama, Iori Kanazawa, Kazuhisa Honma, et al.hepatocyte growth factor (HGF) and interleukin-6 (IL-6) in the supernatants were measured using ELISA kits DHG00B (HGF) and D6050B (IL-6) (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions.

(2) Hepatocyte growth factor may contribute to male protection against pulmonary arterial hypertensionBiology of Sex DifferencesMarch 1, 2026Lejla Medzikovic, Grégoire Ruffenach, Ateyeh Dehghanitafti, Brenda Wong, Ashley Ryder, Mohammad Reza Hatamnejad, Wasila Sun, Leana Esdin, Joshua Eghbali, Adam Brownstein, et al.are described in Tables 2 and 3. ELISA HGF was measured in human plasma using ELISA (R&D Systems #DHG00B) according to the manufacturer’s instructions. Absorbance was measured using a microplate reader (Synergy H1

(3) Spatially resolved multi-omics of human metabolic dysfunction-associated steatotic liver diseaseNature GeneticsNovember 24, 2025Ziyu Li, Gang Luo, Changpei Gan, Huayu Zhang, Ling Li, Xiaoxun Zhang, Xudong Xing, Simeng Hu, Xu Tan, Jingjing Ding, et al.HGF concentrations in the conditioned media were quantified using a Quantikine ELISA Kit (cat. no. DHG00B, R&D Systems), according to the manufacturer’s instructions. Computational analysis Mapping of the

(4) Engineering Regenerative Fibrin Scaffold from Balanced Protein-Concentrate Plasma: Structural and Biochemical CharacterizationPharmaceuticsNovember 5, 2025Diego Delgado, Jon Mercader-Ruiz, Daniel Marijuán-Pinel, Pello Sánchez, Renato Andrade, João Espregueira-Mendes, Llanos Zuloaga, Jorge Knörr, Mikel Sánchez(TGF-β1) (DB100C; Bio-techne Minneapolis, MI, USA), IGF-1 (DG100B; Bio-techne Minneapolis, MI, USA), HGF (DHG00B; Bio-techne Minneapolis, MI, USA) and Platelet-derived GF (PDGF-AB) (DHD00C; Bio-Techne Minneapolis,

(5) The lysosomal cysteine protease cathepsin L promotes stemness and multidrug resistance of non-small cell lung cancer by targeting HGF activatorMolecular and Cellular BiochemistryNovember 3, 2025Hui Shi, Jianyu Xu, Juan Wu, Sha Hu, Xi Chen, Qianfang Hu, Qian Liu, Yanxia Yu, Xinyuan Ding, Wenjuan Wangconcentrations were quantified with commercial human HGFAC (DY1514, R&D Systems, MN, USA) and HGF (DHG00B, R&D Systems) ELISA kits according to the manufacturer’s instructions. Cells were seeded in 6-well plates

(6) Hepatocyte Growth Factor-Mediated Chondrocyte Proliferation Induced by Adipose-Derived MSCs from Osteoarthritis Patients and Its Synergistic Enhancement by Hyaluronic AcidInternational Journal of Molecular SciencesSeptember 23, 2025Samuel Jaeyoon Won, Hyun-Joo Lee, Dae-Yong Kim, Hyeonjeong Noh, Song yi Lee, Ji Ae Yoo, Yoon Sang Jeon, Heebeom Shin, Dong Jin Ryuconditioned media were collected and analyzed using ELISA kits specific for human HGF (R&D Systems, DHG00B), TGF-β1 (DB100C), FGF2 (DFB50), and VEGF (DVE00). All samples were measured in duplicate, and concentrations

(7) A Mechanistic Computational Model of HGF-VEGF Mediated Endothelial Cell Proliferation and Vascular PermeabilityiScienceAugust 15, 2025Rebeca Hannah Hannah de Melo Oliveira, Akash Patil, Brian H. Annex, Arvind P. Pathak, Aleksander S. Popelconcentration of each lysate was quantified using the Quantikine Human HGF ELISA Kit (R&D Systems, Cat# DHG00B) following the manufacturer’s protocol. Method details Model structure: downstream pathways included When

(8) MET pathway inhibition increases chemo-immunotherapy efficacy in small cell lung cancerCell reports. MedicineJuly 15, 2025Del Rey-Vergara R, Galindo-Campos MA, Rocha P, Carpes M, Martínez C, Masfarré L, Menéndez S, Quimis F, Rossell A, Iñañez A, et al.cells. ELISA Serum cytokine levels from SCLC patients were determined using the Human HGF Quantikine ELISA Kit (DHG00B, R&D Systems), following the manufacturer’s instructions. Samples were run in duplicates. Quantification

(9) Exploring the Impact of Extraplatelet Content on Fibrin-Based Scaffold Performance for Regenerative TherapiesInternational Journal of Molecular SciencesJune 21, 2025Daniel Marijuán-Pinel, Jon Mercader-Ruiz, Maider Beitia, Pello Sánchez, Leonor López de Dicastillo, Sergio Gonzalez, João Espregueira-Mendes, Beatriz Aizpurua, Jaime Oraá, Diego Delgado, et al.(DVE00; Bio-techne, Minneapolis, MI, USA), TGF-β1 (DB100C; Bio-techne), IGF-1 (DG100B; Bio-techne), HGF (DHG00B; Bio-techne) and PDGF-AB (DHD00C; Bio-techne). All protein levels were measured by absorbance and the

(10) Human platelet lysate produced from leukoreduction filter contents enables sufficient MSC growthStem Cell Res TherApril 23, 2025Shinobu Wakamoto, Tomoko Furukawa, Masahito Kawabori, Mitsuaki Akino, Shiho Kato, Hisae Fuse, Sumio Ohtsuki, Yoshihiro Torimoto, Miki Fujimura, Shuichi Kinoand were analyzed for the expression of hepatocyte growth factor (HGF) using a Human HGF ELISA kit (DHG00B, R&D Systems, Minneapolis, MN, USA), and subjected to proteomic analysis. HGF expression was normalized

(11) Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regenerationScientific reportsFebruary 6, 2025Sánchez M, Mercader Ruiz J, Marijuán Pinel D, Sánchez P, Fiz N, Guadilla J, Azofra J, Beitia M, Delgado D.(DVE00; Bio-techne, Minneapolis, MI, USA), TGF-β1 (DB100C; Bio-techne), IGF-1 (DG100B; Bio-techne), HGF (DHG00B; Bio-techne), PDGF-AB (DHD00C; Bio-techne), EGF (DEG00; Bio-techne); FGF (DFB50; Bio-techne), BDNF (DBD00;

(12) Ferroptotic Neutrophils Induce Immunosuppression and Chemoresistance in Breast CancerCancer researchFebruary 1, 2025Zeng W, Zhang R, Huang P, Chen M, Chen H, Zeng X, Liu J, Zhang J, Huang D, Lao L.(Invitrogen, #KHL1701), Human IDO ELISA Kit (R&D Systems, #DY6030B), Human HGF ELISA Kit (R&D Systems, #DHG00B), Human TGF-beta1 ELISA Kit (R&D Systems, #DB100C), Human IL-10 ELISA Kit (R&D Systems, #D1000B), Human

(13) A low molecular weight dextran sulphate, ILB®, for the treatment of amyotrophic lateral sclerosis (ALS): An open-label, single-arm, single-centre, phase II trialPLOS ONEJuly 11, 2024Venkataramanan Srinivasan, Victoria Homer, Darren Barton, Abigail Clutterbuck-James, Siân Jenkins, Claire Potter, Kristian Brock, Ann Logan, Donna Smith, Lars Bruce, et al.quantification was carried out using the Quantikine® enzyme-linked immunosorbent assay (ELISA; Cat No: DHG00B, R&D Systems, UK). Patient samples were measured in duplicates at two separate dilutions (1:10 and 1:100)

(14) Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarctionBioactive materialsJuly 1, 2024Zhang J, Li J, Qu X, Liu Y, Sun L, Harada A, Hua Y, Sougawa N, Tabata A, Liu L, et al.(ELISA), including vascular endothelial growth factor (VEGF, DVE00), hepatocyte growth factor (HGF, DHG00B) and basic fibroblast growth factor (β-FGF, DFB50, R&D Systems, Minneapolis, MN, USA, Quantikine ELISA),

(15) Androgen deprivation induces double-null prostate cancer via aberrant nuclear export and ribosomal biogenesis through HGF and Wnt activationNature communicationsFebruary 9, 2024Kim WK, Buckley AJ, Lee DH, Hiroto A, Nenninger CH, Olson AW, Wang J, Li Z, Vikram R, Adzavon YM, et al.

(16) Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat heartsStem cell research & therapyDecember 20, 2023Zhang J, Li J, Qu X, Liu Y, Harada A, Hua Y, Yoshida N, Ishida M, Tabata A, Sun L, et al.(ELISA), including vascular endothelial growth factor (VEGF, DVE00), and hepatocyte growth factor (HGF, DHG00B, R&D Systems, Minneapolis, MN, USA, Quantikine ELISA), and was performed according to the manufacturer's

(17) Fibroblasts are the most suitable cell source for regenerative medicine due to their high intracellular fibroblast growth factor 2 contentBiochemistry and biophysics reportsSeptember 1, 2023Yanagihara M, Matsuno Y, Ueno K, Kurazumi H, Suzuki R, Tanaka T, Hamano K.FGF2, HGF, and VEGF in the samples was measured using human Quantikine ELISA Kits (DFB50 for FGF2, DHG00B for HGF, DVE00 for VEGF; R&D Systems, Inc. Minneapolis, MN, USA) according to the manufacturer's instructions.

(18) Novel rAAV vector mediated intrathecal HGF delivery has an impact on neuroimmune modulation in the ALS motor cortex with TDP-43 pathologyGene therapyAugust 1, 2023Genç B, Nho B, Seung H, Helmold B, Park H, Gözütok Ö, Kim S, Park J, Ye S, Lee H, et al.

(19) High CD142 Level Marks Tumor-Promoting Fibroblasts with Targeting Potential in Colorectal CancerInternational journal of molecular sciencesJuly 18, 2023Soós AÁ, Kelemen A, Orosz A, Szvicsek Z, Tölgyes T, Dede K, Bursics A, Wiener Z.centrifugation (300× g, 5 min), and HGF was detected with ELISA (Bio-Techne, Minneapolis, MN, USA, DHG00B) according to the manufacturer’s protocol. We measured the OD values of the plates on a HiPo MPP-96

(20) Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by ErythropoietinPharmaceuticsMarch 24, 2023Iso Y, Usui S, Suzuki H.

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HGF ELISA