Description
Description: This assay employs a two-site sandwich ELISA to quantitate GTPBP1 in samples. An antibody specific for GTPBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GTPBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GTPBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GTPBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: GTPBP1 is upregulated by interferon-gamma and encodes a protein that is a member of the AGP11/GTPBP1 family of GTP-binding proteins. A structurally similar protein has been found in mouse, where disruption of the gene for that protein had no observable phenotype.
Senju and Nishimura (1997) carried out PCR-based cDNA subtraction and subsequent differential display on mRNA isolated from a human monocytic leukemia cell line, THP-1. They detected a novel gene encoding a protein bearing GTP-binding motifs characteristic of G proteins. They also identified the mouse homolog of this gene and designated the gene GP1. The predicted 584-amino acid human protein is 97% identical to the mouse homolog