Fig 1: Overexpression of miR-143 improves sepsis-induced inflammation and cardiac function. (A) Levels of miR-143 in the sham group, CLP group, CLP + miR-NC group and CLP + miR-143 mimics group. (B) LVPP, (C) LVEDP, (D) CK, (E) CK-MB and (F) cTn-I levels in the CLP + miR-NC group and CLP + miR-143 mimics group. Serum levels of (G) C5, C5a, (H) TNF-α, IL-1β, IL-6, IL-10, IL-17 and IFN-γ in the CLP + miR-NC group and CLP + miR-143 mimics group. PVT1, plasmacytoma variant translocation gene 1; CLP, cecal ligation and puncture; miR, microRNA; NC, negative control; LVPP, left ventricular peak pressure; LVRFP, left ventricular end diastolic pressure; CK, creatine kinase; cTn-I, cardiac troponin I; TNF-α, tumor necrosis factor-α; IL, interleukin; IFN-γ, interferon-γ. *P<0.05.
Fig 2: Upregulation of lncRNA H19 reduces pulmonary inflammation and apoptosis in septic rats. (A) lncRNA H19 downregulated caspase-3, caspase-9, Bax and upregulated Bcl-2. (B) Upregulation of lncRNA H19 reduced TNF-α, IL-6 and IL-17 in pulmonary tissue. (C) Upregulation of lncRNA H19 reduced TNF-α, IL-6 and IL-17 in serum. (D) Western blotting of caspase-3, caspase-9, Bax, Bcl-2, TNF-α, IL-6 and IL-17. *P<0.05 vs. the control group; #P<0.05 vs. the model group. lncRNA, long non-coding RNA; Bax, BCL2-associated X; Bcl-2, B-cell lymphoma-2; TNF-α, tumor necrosis factor-α; IL, interleukin.
Fig 3: QRHX inhibits PI3K/Akt signal pathway and down-regulates the level of inflammatory cytokines. (A) Inflammatory factor levels of MCP-1, IL-17A, TNF-α and IL-1β. (B) Protein expression levels of p-PI3K, PI3K, p-Akt and Akt. (C) Quantitative analysis of p-PI3K, PI3K, p-Akt and Akt using ImageJ software. Data were expressed as mean ± SEM. **p < 0.01 vs. Normal group, #p < 0.05 and ##p < 0.01 vs. Model group.
Fig 4: PVT1 regulates sepsis-induced inflammation and cardiac function. (A) LVPP, (B) LVEDP, (C) CK, (D) CK-MB and (E) cTn-I levels in the sham group, CLP group, CLP + LV-vector group and CLP + LV-shPVT1 group. Serum levels of (F) C5, C5a, (G) TNF-α, IL-1β, IL-6, IL-10, IL-17 and IFN-γ in the sham group, CLP group, CLP + LV-vector group and CLP + LV-shPVT1 group. PVT1, plasmacytoma variant translocation gene 1; CLP, cecal ligation and puncture; LV, lentivirus; sh, short hairpin RNA; LVPP, left ventricular peak pressure; LVEDP, left ventricular end diastolic pressure; CK, creatine kinase; cTn-I, cardiac troponin I; TNF-α, tumor necrosis factor-α; IL, interleukin; IFN-γ, interferon-γ. *P<0.05.
Supplier Page from Abcam for Rat IL-17A ELISA Kit