MojoSort™ Human Pan B Cell Isolation Kit 480081 from BioLegend

Product Specs
ItemMojoSort™ Human Pan B Cell Isolation Kit
CompanyBioLegend
Price $249.00Supplier Page View Company Product Page
Catalog Number480081
Quantity20 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phophate buffer solution containing 0.09% sodium azide, pH 7.2.
Particle: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityHuman
Detection TargetPan B
Cell Type (standardized)B Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) The N-terminal region of malaria vaccine candidate Plasmodium falciparum asparagine-rich merozoite antigen is immunodominant and targeted by polyreactive antibodiesbioRxivDecember 13, 2025Rolando Garza, Jeffrey M. Marchioni, Jared D. Honeycutt, Nicholas K. Hurlburt, Caroline Torres, Anakaren Garcia, Eva Loranc, Emily Yemington, Dalton Towers, Isaac Ssewanyana, et al.aggregated cells. B cells were then isolated using a MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to manufacturer’s instruction. The isolated B cells were washed with PBS and subsequently

(2) Monocyte/macrophage-derived interleukin-15 mediates the pro-inflammatory phenotype of CD226(+) B cells in type 1 diabetesEBioMedicineOctober 1, 2025Li J, Liang X, Zhao M, Luo W, Huang J, Xiao Y, Huang J, Zhao B, Zhou Z.NOD mouse splenic B cells were enriched with MojoSort™ Human Pan B Cell Isolation Kit (BioLegend, Cat#480082) and MojoSort™ Mouse Pan B Cell Isolation Kit II (BioLegend, Cat#480088). The sorting purity was > 95.0%.

(3) KSHV vIL6 Inhibits Functional B Cell Maturation During De Novo InfectionJ Med VirolJuly 7, 2025Wajd Zakir, Jessica M. Osborn, Jennifer E. Totonchy(7270‐IL‐010/CF). The Robosep Human naïve isolation kit was purchased from Biolegend (San Diego, CA) (BL 480082). 2.2 Isolation of Primary B Lymphocytes Deidentified human tonsil specimens were collected from the

(4) Microbiota‐Derived Inosine Suppresses Systemic Autoimmunity via Restriction of B Cell Differentiation and MigrationAdvanced ScienceApril 28, 2025Lingyu Gao, Yuhan Zhang, Zhi Hu, Shengwen Chen, Qiaolin Wang, Yong Zeng, Huiqi Yin, Junpeng Zhao, Yijing Zhan, Changxing Gao, et al.H‐89 dihydrochloride,MCE,HY‐15979A Rapamycin,MCE,HY‐10219 inosine,MCE,HY‐N0092 LW6,MCE,HY‐10219 PD98059,MCE,HY‐12028 KLH,Sigma‐Aldrich,9013‐72‐3 Critical commercial assays,, MojoSort™ Human Pan B Cell Isolation Kit,Biolegend,480082 MojoSort™ Mouse Pan B Cell Isolation Kit II,Biolegend,480088 ChamQ Universal SYBR qPCR Master Mix,Vazyme,Q711‐02 HiScript

(5) Sp140L Is a Novel Herpesvirus Restriction FactorbioRxivDecember 18, 2024Jana M. Cable, Wiyada Wongwiwat, Jenna C. Grabowski, Robert E. White, Micah A. LuftigEasySep Human Pan-B Cell Enrichment kit (STEMCELL Technologies #19554) or MojoSort Human Pan B Isolation kit (Biolegend # 480082). To obtain isolated naïve and memory B cells for SeahorseXF, the EasySep Human Memory B Cell Isolation kit

(6) Broadly inhibitory antibodies against severe malaria virulence proteinsNatureDecember 5, 2024Raphael A. Reyes, Sai Sundar Rajan Raghavan, Nicholas K. Hurlburt, Viola Introini, Sebastiaan Bol, Ikhlaq Hussain Kana, Rasmus W. Jensen, Elizabeth Martinez-Scholze, María Gestal-Mato, Borja López-Gutiérrez, et al.EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions. B cells were washed with PBS, centrifuged (250 × g, 5

(7) 2D4, a humanized monoclonal antibody targeting CD132, is a promising treatment for systemic lupus erythematosusSignal Transduction and Targeted TherapyNovember 17, 2024Huiqi Yin, Liming Li, Xiwei Feng, Zijun Wang, Meiling Zheng, Junpeng Zhao, Xinyu Fan, Wei Wu, Lingyu Gao, Yijing Zhan, et al.from the fresh peripheral blood of healthy donors by negative magnetic selection (BioLegend, Cat. No. 480082 & 480130). Purified B and T cells were cultured at 0.5 million cells/well in 96-well plates in 100 uL

(8) B cell receptor dependent enhancement of dengue virus infectionPLOS PathogensOctober 31, 2024Chad Gebo, Céline S. C. Hardy, Benjamin D. McElvany, Nancy R. Graham, Joseph Q. Lu, Shima Moradpour, Jeffrey R. Currier, Heather Friberg, Gregory D. Gromowski, Stephen J. Thomas, et al.isolated from flavivirus-naïve healthy donors using MojoSort Human Pan B cell Isolation Kit (BioLegend, 480082) were then added and incubated for 24 hours. Supernatants were harvest at 24hrs for cytokine analysis,

(9) Differences in phenotype between long-lived memory B cells against Plasmodium falciparum merozoite antigens and variant surface antigensPLOS PathogensOctober 28, 2024Raphael A. Reyes, Louise Turner, Isaac Ssewanyana, Prasanna Jagannathan, Margaret E. Feeney, Thomas Lavstsen, Bryan Greenhouse, Sebastiaan Bol, Evelien M. BunnikEasySep Human B Cell Isolation Kit (StemCell, #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, # 480082) according to the manufacturer’s instructions. Staining for spectral flow analysis C-terminally biotinylated

(10) Epstein-Barr virus suppresses N(6)-methyladenosine modification of TLR9 to promote immune evasionThe Journal of biological chemistryMay 1, 2024Zhang X, Li Z, Peng Q, Liu C, Wu Y, Wen Y, Zheng R, Xu C, Tian J, Zheng X, et al.centrifugation. Primary B cells were isolated from PBMCs by MojoSort human Pan B cell Isolation Kits (480081, BioLegend) and were cultured in RPMI 1640 medium supplemented with 10% FBS. All cell lines were grown

(11) Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironmentNature communicationsDecember 20, 2023Lu X, Luo Y, Nie X, Zhang B, Wang X, Li R, Liu G, Zhou Q, Liu Z, Fan L, et al.(STEMCELL Technologies). Total CD19 + B cells were prepared with MojoSort™ Human Pan B Cell Isolation Kit (Catalog:480082, Biolegend). B cells were cultured in full RPMI 1640 medium in the presence of 10 ng/mL recombinant

(12) Modulation of the Itaconate Pathway Attenuates Murine LupusArthritis & rheumatology (Hoboken, N.J.)December 1, 2022Blanco LP, Patino-Martinez E, Nakabo S, Zhang M, Pedersen HL, Wang X, Carmona-Rivera C, Claybaugh D, Yu ZX, Desta E, et al.isolated with Ficoll gradient and treated with MojoSort Human Pan B Cell Isolation Kit (BioLegend, #480081). CD19+ B cell purity was above 90%. Purified CD19+ B cells (50,000 cells/ 200 ul) were cultured alone

(13) Suppression of DC-SIGN and gH Reveals Complex, Subset-Specific Mechanisms for KSHV Entry in Primary B LymphocytesVirusesJuly 31, 2021Palmerin N, Aalam F, Nabiee R, Muniraju M, Ogembo JG, Totonchy J.were counted and total B cells were isolated using Mojosort™ Human Pan B cell isolation kit (Biolegend 480082) according to manufacturer’s instructions. Bound cells that were non-B cells were retained and maintained

(14) Multifunctional barcoding with ClonMapper enables high-resolution study of clonal dynamics during tumor evolution and treatmentNature cancerJuly 1, 2021Gutierrez C, Al'Khafaji AM, Brenner E, Johnson KE, Gohil SH, Lin Z, Knisbacher BA, Durrett RE, Li S, Parvin S, et al.Program, Project 080918). Viable CD19+ cells were purified by FACS or B-cell selection (BioLegend, 480082). Cells were resuspended into PBS–0.04% BSA at 1 × 106 cells ml−1 and processed using the single cell

(15) Massively parallel single-cell mitochondrial DNA genotyping and chromatin profilingNature biotechnologyApril 1, 2021Lareau CA, Ludwig LS, Muus C, Gohil SH, Zhao T, Chiang Z, Pelka K, Verboon JM, Luo W, Christian E, et al.B lymphocytes were isolated using the negative selection Mojosort Human Pan B Cell Isolation Kit (Biolegend, 480082) and CD19 negative immune cells were isolated from a separate aliquot using the positive selection Mojosort Human CD19 selection Kit

(16) A hotspot mutation in transcription factor IKZF3 drives B cell neoplasia via transcriptional dysregulationCancer cellMarch 8, 2021Lazarian G, Yin S, Ten Hacken E, Sewastianik T, Uduman M, Font-Tello A, Gohil SH, Li S, Kim E, Joyal H, et al.(Miltenyi Biotec; 130-090-101). CLL/B cells were purified using CD19 negative selection kit (Biolegend; 480082) and CD19 negative immune cells were purified using CD19 positive magnetic beads (Biolegend; 480106)

(17) CD226 Deletion Reduces Type 1 Diabetes in the NOD Mouse by Impairing Thymocyte Development and Peripheral T Cell ActivationFrontiers in immunologyJanuary 1, 2020Shapiro MR, Yeh WI, Longfield JR, Gallagher J, Infante CM, Wellford S, Posgai AL, Atkinson MA, Campbell-Thompson M, Lieberman SM, et al.(RM4-4, BioLegend Cat# 116007, RRID:AB_11147758), CD8a-eFluor450 (53-6.7, Thermo Fisher Scientific Cat# 480081-82, RRID:AB_1272198) or –BV711 (53-6.7, BioLegend Cat# 100759, RRID:AB_2563510), NKp46-FITC (29A1.4, BioLegend

(18) microRNA-630 Regulates Underglycosylated IgA1 Production in the Tonsils by Targeting TLR4 in IgA NephropathyFrontiers in immunologyJanuary 1, 2020Liu C, Ye MY, Yan WZ, Peng XF, He LY, Peng YM.Human CD4 T cell, CD8 T cell, pan B cell, and Pan Monocyte Cell Isolation Kit (Cat No. 480009, 480011, 480081, 480059, BioLegend Inc. San Diego, CA, USA), respectively, according to the manufacturer’s instructions. Luciferase Reporter Assay

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MojoSort™ Human Pan B Cell Isolation Kit from BioLegend

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MojoSort™ Human Pan B Cell Isolation Kit from BioLegend

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