Human IFN-beta DuoSet ELISA DY814-05 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IFN-beta DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $450.00Supplier Page View Company Product Page
Catalog NumberDY814-05
Quantity1 Kit (for 5 Plates)
ApplicationsELISA
Detection Range7.81 - 500 pg/mL
ReactivityHuman
Detection TargetIFN-beta
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterferon beta 1
NCBI Gene AliasesIFB, IFF, IFN-beta, IFNB

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Protocol to study the role of endogenously produced itaconate using CRISPR-Cas9 technology in THP-1 cellsSTAR ProtocolsMarch 1, 2026Luke A. Bourner, Katie A. Acken, Haiyan Long, Linda A. Chung, Kenneth D. Roth, Frank C. Dorseyassays,Critical commercial assays,Critical commercial assays ,, Human IFN-beta DuoSet ELISA,R&D Systems,Cat#DY814-05 SG Cell Line Nucleofector X Kit,Lonza Bioscience,Cat#V4XC-3012 TMB Substrate Kit,Thermo Scientific,Cat#34021 ,, Experimental models

(2) Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populationsCell ReportsFebruary 24, 2026Xiaowen Hu, Jie Huang, Jiao Yuan, Yanrong Sun, Yuxin Wang, Zhongyi Hu, Junjie Jiang, Zhiling Wang, Bingwei Wang, Meixiao Long, et al.rest period. Cell culture supernatants were collected, and Human IFN-beta DuoSet ELISA kit (Cat No: DY814–05, R&D Systems) was utilized to measure IFN-β production as per the manufacturer’s instructions. Additionally,

(3) Toll like receptor agonist effects on human CD8+ T cell activation and expression of T cell checkpoint receptorsHum Vaccin ImmunotherDecember 18, 2025Donghwan Jeon, Jena E. Moseman, Ethan Hill, Douglas G. McNeelthen evaluated for the presence of IL-12p70 or IFNβ by ELISA (R&D systems, Minneapolis, MN DY1270-05, DY814-05), according to the manufacturer’s instructions. Statistical analysis Group mean comparisons were performed

(4) RIG-I drives protective type I interferon production by glial cells in response to Neisseria meningitidis and Streptococcus pneumoniae challengeFrontiers in ImmunologyNovember 18, 2025Krishna J. Majithia, M. Brittany JohnsonBD Biosciences; Cat #554400, #554402, RRID: AB_398549, AB_395368) (murine)] and IFN-β [R&D Systems; DY814-05 (human), BD Biosciences; 519202, 508105 (murine)] were determined using commercially available antibody

(5) Mycobacterial ESAT-6 triggers cGAS-STING pathway via the GSDMD-NT dependent mitochondria dysfunctionVirulenceOctober 15, 2025Yang Yang, Jinxia Xu, Yanping Hu, Xinxin Zang, Yunjie Li, Ahui Xu, Hexiang Jiang, Jingyan Fan, Luqing Cui, Bei Huang, et al.ΔCt(control group). ELISA Levels of interferon-β (IFN-β) in supernatants were measured using ELISA kits (DY814-05, R&D Systems) according to the manufacturer’s instructions. Live cell confocal imaging A total of

(6) NAT10 Suppresses RNA Sensing Induced IFN-β Transactivation to Promote Viral Infection via Interfering with IRF3 ActivitiesbioRxivSeptember 11, 2025Youngmin Park, Jinshan He, Eleya Eleya, Zhenyu Zhenyu, Guillaume N Fiches, Dawei Zhou, Emily G Watters, Zhixian M He, Kateepe ASN Shanaka, Thurbu T Lepcha, et al.(Cat. # 1705062, Bio-Rad). Enzyme-linked immunosorbent assay (ELISA) Human IFN-β DuoSet ELISA (Cat. # DY814–05, R&D Systems) was used according to the manufacturer’s protocols. Briefly, supernatants were collected

(7) Plasmacytoid dendritic cells in the intestine preferentially produce interferon lambda at homeostasis contributing to tonic localized innate immune responsesbioRxiv : the preprint server for biologyAugust 28, 2025Constant DA, Van Winkle JA, Norwood PA, Mishra G, Meyer KA, Laney ME, Madden SR, Griffith A, Rodrigues PF, Savan R, et al.

(8) The lncRNA EPIC1 suppresses dsRNA-induced type I IFN signaling and is a therapeutic target to enhance TNBC response to PD-1 inhibitionScience SignalingAugust 12, 2025Dhamotharan Pattarayan, Yue Wang, Zehua Wang, Sihan Li, Xiaofei Wang, Yuang Chen, Yifei Wang, Chien-Yu Chen, Avishek Bhuniya, Ghanshyam Singh Yadav, et al.according to the manufacturer’s protocol. The human IFN-β ELISA kit was purchased from R&D Systems (#DY814–05). The optical density was determined using a Bio-Rad xMark Microplate Absorbance Spectrophotometer. In vitro

(9) Palbociclib and dsRNA sensor co-operate to enhance anti-cancer effects through ER stress and modulation of immune evasionNat CommunMay 25, 2025Victoria Roulstone, Joan Kyula-Currie, James Wright, Emmanuel C. Patin, Isaac Dean, Lu Yu, Aida Barreiro-Alonso, Miriam Melake, Jyoti Choudhary, Richard Elliott, et al.compounds STF-083010 (IRE1α inhibitor) was used at 20 µM and IFNβ was measured using the DuoSet ELISA (DY814-05, R&D Systems) as per manufacturer’s instructions. Statistical analysis Statistical analysis was performed

(10) Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potencyNature biotechnologyMay 1, 2025McGee JE, Kirsch JR, Kenney D, Cerbo F, Chavez EC, Shih TY, Douam F, Wong WW, Grinstaff MW.

(11) Bronchial smooth muscle extracellular vesicles interfere with bronchial epithelium metabolism and function in asthmaiScienceApril 29, 2025Elisa Celle, Amine Chahin, Fabien Beaufils, Guillaume Cardouat, Edmée Eyraud, Clément Bouchet, Marilyne Campagnac, Olga Ousova, Hugues Begueret, Matthieu Thumerel, et al.kit,Bio-Techne,NBP2-76703 Human MUC5B ELISA kit,Bio-Techne,NBP2-76705 Human IFN-beta ELISA,Bio-Techne,DY814-05 Human IFN-Lambda1/3 ELISA,Bio-Techne,DY1598B Human IFN-gamma ELISA,Bio-Techne,DY285B Human TSLP ELISA,Bio-Techne,DY1398 Human

(12) Cytokine profiles dynamics in COVID-19 patients: a longitudinal analysis of disease severity and outcomesScientific reportsApril 23, 2025Ghaffarpour S, Ghazanfari T, Ardestani SK, Naghizadeh MM, Vaez Mahdavi MR, Salehi M, Majd AMM, Rashidi A, Chenary MR, Mostafazadeh A, et al.

(13) Passage-attenuated Powassan virus LI9P protects mice from lethal LI9 challenge and links envelope residue D308 to neurovirulencemBioApril 9, 2025Himmler GE, Mladinich MC, Conde JN, Gorbunova EE, Lindner MR, Kim HK, Mackow ER.

(14) A non-structural protein 1 substitution of dengue virus enhances viral replication by interfering with the antiviral signaling pathwayJournal of biomedical scienceFebruary 20, 2025Hee JR, Cheng D, Chen YH, Wang SH, Chao CH, Huang SW, Ling P, Wan SW, Chang CP, Chu JJH, et al.

(15) MERS-CoV-nsp5 expression in human epithelial BEAS 2b cells attenuates type I interferon production by inhibiting IRF3 nuclear translocationCellular and Molecular Life SciencesDecember 1, 2024Y. Zhang, S. Kandwal, D. Fayne, N. J. Stevensonanother 24 h. Supernatants were harvested and cytokine concentrations of IFN-β were quantified by ELISA (DY814-05, R&D Systems) according to the manufacturer’s guideline. Luciferase reporter assay BEAS 2b cells cultured

(16) UBXN9 governs GLUT4-mediated spatial confinement of RIG-I-like receptors and signalingNature ImmunologyDecember 1, 2024Andrew G. Harrison, Duomeng Yang, Jason G. Cahoon, Tingting Geng, Ziming Cao, Timofey A. Karginov, Youjia Hu, Xin Li, Conner C. Chiari, Yibing Qyang, et al.were assessed using DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems, Cat #DY814-05). The IFN-β data are presented as picograms per milliliter. For western blotting analysis, cells were

(17) The transcription regulators ZNF750 and LSD1/KDM1A dampen inflammation on the skin’s surface by silencing pattern recognition receptorsImmunityOctober 1, 2024Ye Liu, Yifang Chen, Uyanga Batzorig, Jingting Li, Celia Fernández-Méndez, Samiksha Mahapatra, Fengwu Li, Shebin Sam, Tatsuya Dokoshi, Seung-Phil Hong, et al.Kit,Illumina,RS-122–2001 Maxima cDNA synthesis kit,Thermo Fisher,K1642 DuoSet ELISA Development System,R&D Systems,DY814–05 Development System,, Cyto-Fast Fix/Perm Buffer Kit,Biolegend,426803 Deposited Data,Deposited Data,Deposited

(18) FAT10 inhibits TRIM21 to down-regulate antiviral type-I interferon secretionLife Science AllianceSeptember 1, 2024Kritika Saxena, Katharina Inholz, Michael Basler, Annette Aichemrespectively. A classical sandwich-ELISA for IFNβ was performed according to the manufacturer’s protocol (DY814-05; R&D System) with the supernatants of the infected cells. Uninfected cells were used as negative control.

(19) The Impact of Modern Bone Markers in Multiple Myeloma: Prospective Analyses Pre and Post-First Line TreatmentCurrent Issues in Molecular BiologyAugust 24, 2024Vlad Stefan Pop, Mihaela Iancu, Adrian Bogdan Țigu, Anda Adam, Gheorghe Tomoaia, Anca Daniela Farcas, Anca Simona Bojan, Andrada ParvuGmbH, Grodig, Austria) at 450 nm. IL-6 (cat nr. DY206-05), TNF-alpha (cat nr. DY210-05), and IFN-beta (DY814-05) were quantified using three duo-set ELISA assay kits from R&D systems (R&D Systems, Minneapolis, MN,

(20) Lysosomal endonuclease RNase T2 and PLD exonucleases cooperatively generate RNA ligands for TLR7 activationImmunityJuly 9, 2024Marleen Bérouti, Katja Lammens, Matthias Heiss, Larissa Hansbauer, Stefan Bauernfried, Jan Stöckl, Francesca Pinci, Ignazio Piseddu, Wilhelm Greulich, Meiyue Wang, et al.assays,Critical commercial assays,Critical commercial assays ,, Human IFN-βELISA Set,R&D System,Cat# DY814-05 Human IFN-α2 ELISA Set,R&D System,Cat# DY9345-05 Human IL-6 ELISA Set,BD Biosciences,Cat# 555220 Human TNF ELISA Set

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IFN-beta ELISA

Human IFN-beta DuoSet ELISA

Human IFN-beta DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human IFN-beta DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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