GoScript Reverse Transcription Mix, Random Primers from Promega

Product Specs
ItemGoScript Reverse Transcription Mix, Random Primers
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA2800
Quantity50 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signalsDevelopmental BiologyMay 1, 2026Pamela Kairath, Pei Xuan Er, Sean Wilson, Irene Ghobrial, Jessica M. Vanslambrouck, Yi-Hsien Yi-Hsien, Pruett-Miller Shondra Pruett-Miller, Sanjay Jain, Melissa H. LittleScientific). Equal amounts of RNA were used to make cDNA using the GoScript reverse transcription system (Cat#A2800, Promega), following manufacturer’s instructions. Quantitative PCR were performed using GoTaq qPCR master mix

(2) Circular RNA circATM binds PARP1 to suppress Wnt/β-catenin signaling and induce cell cycle arrest in gastric cancer cellsJournal of Advanced ResearchFebruary 1, 2026Xiaohui Zhu, Xiaojing Zhang, Ying Qin, Yang Chen, Xianling Feng, Shiqi Deng, Fan Hu, Yuan Yuan, Xiaonuan Luo, Kaining Du, et al.Corp., ON, Canada). Reverse transcription was performed using GoScriptTM Reverse Transcription Mix (Cat. A2800, Promega, Beijing, China), while GoTaq® RT-qPCR Master Mix (Cat. A6001, Promega, Beijing, China) was

(3) Proliferation, migration, and differentiation of circulating ILC precursors in HBV infection-associated fibrosisEuropean Journal of Medical ResearchJanuary 5, 2026Qin Zhu, Fang-yuan Chen, Shi-qin Li, Cheng-zhao Weng, Si-qi Wang, Lin-lin Zheng, Yong-yu Yang, Li Xie, Jian Wu, Wei Jiangthen converted to cDNA using Promega GoScript™ Reverse Transcription Mix and Random Primers (Promega, #A2800, Wisconsin, USA). qRT-PCR was performed with Eastep™ qPCR Master Mix (Promega, #LS2068, Wisconsin, USA)

(4) Multi-omics-informed hydrogel design: modulating IL-6 to reduce endoplasmic reticulum stress in bone regenerationBioact MaterOctober 15, 2025Jiannan Zhou, Jingtao Dai, Shixian Hu, Cancan Qi, Jiahao Chen, Wentai Zhang, Dorothea Alexander, An Li, Yin Xiao, Ping Li(EZB-RN4, EZBioscience, US). Total RNA was isolated with the GoScript™ Reverse Transcription System Kit (A2800, Promega, US), and post-transcription cDNA synthesis was performed for real-time quantitative polymerase chain reaction

(5) MAP3K13-232aa encoded by circMAP3K13 enhances cisplatin-induced pyroptosis by directly binding to IKKα in gastric adenocarcinomaCell Death & DiseaseSeptember 1, 2025Kaining Du, Xiaojing Zhang, Ying Qin, Huizhen Ma, Chuhan Bing, Shiqi Deng, Yang Chen, Jiequan Qin, Shanshan Chang, Siyu Xiao, et al.was reverse-transcribed into cDNA using the GoScript Reverse Transcription Mix (Promega, Cat. Nos. A2800 and A6002). Quantitative real-time PCR was performed using the GoTaq qPCR Master Mix (Cat. Nos. A6001

(6) Mechanical response microRNA-145a-5p alleviates osteoarthritis by inhibiting inflammation and promoting chondrogenesisPeerJAugust 19, 2025Jingke Du, Zhen Zhang, Danyang Bai, Ting Zhu, Dong JiangcDNA synthesis, total RNA samples (2 μg) were reverse transcribed with GoScript™ Reverse Transcriptase (A2800; Promega, Madison, WI, USA) following the manufacturer’s protocol. The reaction system (20 μL final

(7) Plumbagin Triggers Cuproptosis in Hepatocellular Carcinoma (HCC) via the DNA-Methyltransferase 1 (DNMT1)/microRNA-302a-3p (miR-302a-3p)/ATPase Copper Transporting Beta (ATP7B) AxisMedComm (2020)August 3, 2025Chuyu Wang, Hao Wang, Chong Wang, Tongtong Tian, Anli Jin, Yu Liu, Ran Huo, Te Liu, Baishen Pan, Wei Guo, et al.RNA were reverse‐transcribed into first‐strand cDNA using a reverse transcription kit (Promega, USA; A2800). Quantitative polymerase chain reaction (qPCR) was performed to assess mRNA expression using the Go

(8) DNA 5mC methylation inhibits the formation of G-quadruplex structures in the genomeGenome BiolJuly 11, 2025Kangkang Niu, Lijun Xiang, Xiaojuan Zhang, Xiaoyu Li, Tingting Yao, Jin Li, Chu Zhang, Junpeng Liu, Yuling Peng, Guanfeng Xu, et al.Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800, Promega, WI, USA) according to the manual. The mRNA levels of individual genes were normalized to the

(9) In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signalsbioRxivJune 3, 2025Pamela Kairath, Pei Xuan Er, Sean Benjamin Wilson, Irene T Ghobrial, Jessica M. Vanslambrouck, Yi-Hsien Yi-Hsien, Shondra M. Pruett-Miller, Sanjay Jain, Melissa H. LittleScientific). Equal amounts of RNA were used to make cDNA using the GoScript reverse transcription system (Cat#A2800, Promega), following manufacturer’s instructions. Quantitative PCR were performed using GoTaq qPCR master mix

(10) LncRNA SNHG15 promotes angiogenesis and improves cardiac repair after myocardial infarction through MiR-665-mediated KDR expressionCell Mol Life SciMay 26, 2025Xiaoyun Liang, Shangyu Liu, Gang Liu, Qiankun Fan, Fangfang Ma, Yajuan Yin, ZhaoMing Li, Yuming Wu, Mingqi Zhengof RNA was reverse transcribed into cDNA using the GoScript™ Reverse Transcription System (Cat. No. A2800, Promega). The resulting cDNA samples were subjected to RT-qPCR on a LightCycler® 480 Instrument II

(11) circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPMInt J OncolJanuary 14, 2025Yang Zhang, Jian Wang, Ruiliang Quan, Lihua Lyutotal tissues/cell RNA. RNA was converted to cDNA using GoScript Reverse Transcription Mix (cat. no. A2800, Promega Corporation) according to the manufacturer's instructions. The GoTaq qPCR Master Mix (cat.

(12) Prostaglandin I2 signaling prevents angiotensin II-induced atrial remodeling and vulnerability to atrial fibrillation in miceCellular and Molecular Life SciencesDecember 1, 2024Yue Zhang, Meng Yuan, Wenbin Cai, Weiyan Sun, Xuelian Shi, Daiqi Liu, Wenhua Song, Yingqun Yan, Tienan Chen, Qiankun Bao, et al.Promega, Madison, WI, USA). RNA samples were reverse-transcript with SuperScript and random primers (A2800; Promega, Madison, WI, USA). Real-time PCR involved use of the SYBR Green qPCR Master Mix (A6001; Promega,

(13) Targeting BRD4 mitigates hepatocellular lipotoxicity by suppressing the NLRP3 inflammasome activation and GSDMD-mediated hepatocyte pyroptosisCellular and Molecular Life SciencesDecember 1, 2024Fangyuan Chen, Shuyu Li, Min Liu, Cheng Qian, Zhiyin Shang, Xu Song, Wei Jiang, Chuantao TuPlus from Takara (#108-95-2, Tokyo, Japan); GoScript™ Reverse Transcription Mix and Random Primers (#A2800) and Eastep™ qPCR Master Mix (#LS2068) from Promega (Wisconsin, USA); Viability dye 506 (#62210-00)

(14) Elucidating the role of CYFIP2 in conferring cisplatin resistance in esophageal squamous cell carcinomaScientific ReportsNovember 7, 2024Peipei Zhang, Weiguang Zhang, Zhinuan Hong, Junfei Jiang, Ningzi Wu, Jihong Lin, Mingqiang Kang(Thermo Fisher Scientific, USA). Subsequently, 1 μg of RNA was used for reverse transcription (Promega A2800, USA). The reaction was set up in triplicate, with 2 μL of cDNA from each well used for RT-qPCR reactions.

(15) iPLA2β loss leads to age-related cognitive decline and neuroinflammation by disrupting neuronal mitophagyJournal of NeuroinflammationSeptember 18, 2024Li Jiao, Wenxin Shao, Wenqi Quan, Longjiang Xu, Penghui Liu, Jinling Yang, Xiaozhong PengPromega). Reverse transcription was performed using the GoScriptTM Reverse Transcription Mix kit (cat#A2800, Promega). q-RT-PCR was performed utilizing the GoTaq® qPCR Master Mix (cat#A6001, Promega) on a Bio-Rad

(16) Targeting the Cdc2‐like kinase 2 for overcoming platinum resistance in ovarian cancerMedComm (2020)April 13, 2024Yinan Jiang, Shuting Huang, Lan Zhang, Yun Zhou, Wei Zhang, Ting Wan, Haifeng Gu, Yi Ouyang, Xiaojing Zheng, Pingping Liu, et al.Nanodrop 2000 spectrophotometer (Thermo Fisher). Total RNA (2 µg) was reversely transcribed using GoScript™ Master Mix (#A2800; Promega). The mRNA levels were detected by Color SYBR Green qPCR Mix (#A0012; EZBioscience) and performed

(17) The MYBL2-CCL2 axis promotes tumor progression and resistance to anti-PD-1 therapy in ovarian cancer by inducing immunosuppressive macrophagesCancer cell internationalOctober 21, 2023Pan B, Wan T, Zhou Y, Huang S, Yuan L, Jiang Y, Zheng X, Liu P, Xiang H, Ju M, et al.Nanodrop 2000 spectrophotometer (Thermo Fisher). Total RNA (2 µg) was converted to cDNA using the GoScript™ Master Mix (A2800, Promega, Wisconsin, USA). The transcripts were quantified by real-time qPCR using a LightCycle 480

(18) Arsenic trioxide-induced apoptosis contributes to suppression of viral reservoir in SIV-infected rhesus macaquesMicrobiology SpectrumOctober 17, 2023Yizi He, Chunxiu Wu, Zijian Liu, Yudi Zhang, Fengling Feng, Zihan Lin, Congcong Wang, Qing Yang, Ziyu Wen, Yichu Liu, et al.then reverse transcribed using GoScript Reverse Transcription Mix, Random Primers (Promega, Cat. No. A2800). The first round of PCR was performed using Premix Taq (Takara, Cat. No. R004A). The following second

(19) Tau protein modulates an epigenetic mechanism of cellular senescence in human SH-SY5Y neuroblastoma cellsFrontiers in Cell and Developmental BiologyOctober 3, 2023Claudia Magrin, Martina Bellafante, Martina Sola, Ester Piovesana, Marco Bolis, Luciano Cascione, Sara Napoli, Andrea Rinaldi, Stéphanie Papin, Paolo Paganetti(15596026, Invitrogen) and cDNA synthesis using the GoScript Reverse Transcription Mix Random Primers (A2800, Promega) were done according to the instructions of the manufacturer. Amplification was performed with

(20) Diosmetin alleviates acute lung injury caused by lipopolysaccharide by targeting barrier functionInflammopharmacologyAugust 1, 2023Xia J, Li J, Deng M, Yin F, Liu J, Wang J.Med Chem Express (NJ, USA). GoScript™ Reverse Transcription kit (A2790) and GoTaq qPCR Master Mix (A2800) were purchased from Promega (Madison, WI, USA). ABTS kit (S0121), RIPA buffers (P0013C), BCA protein assay

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