Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) P-3086 from EpiGentek

Product Specs
ItemEpigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)
CompanyEpiGentek
Price Supplier Page View Company Product Page
Catalog NumberP-3086
Quantity48 Assays, 96 Assays
Sample TypePurified Enzyme, Nuclear Extracts
Research AreaDNA Methylation

EpiGentek

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Farmingdale, NY 11735

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(1) Pyruvate carboxylase promotes SREBP1a-mediated lipid synthesis in epithelial ovarian cancerCommunications BiologyMay 14, 2026Xinyun Huang, Huan Shi, Weiyan Shan, Jingxia Zhang, Qiaoping Xu, Hongkai Shang5hmC-Hydroxylase TET activity was measured using the 5hmC-Hydroxylase TET Activity Assay Kit (Epigentek, P-3086). Nuclear extracts were prepared from EOC cells using the Nuclear Extraction Kit (Epigentek, OP-0002).

(2) Effects of Methylglyoxal on Intestinal Cells: Insights on Epigenetic Regulatory EnzymesIUBMB LifeDecember 8, 2025Camilla Morresi, Giulia Feliziani, Luisa Bellachioma, Christian Giommi, Rosita Gabbianelli, Laura Bordoni, Gianna Ferretti, Tiziana Bacchetti, Elisabetta Damianiand DNMTs enzymes was evaluated by the Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (P‐3086, Epigentek, Farmingdale, NY, USA), Epigenase HDAC Activity/Inhibition Direct Assay Kit (P‐4034, Epigentek),

(3) AKG-TET axis is central to senescence plasticityiScienceDecember 1, 2025Shin Akakura, Siamak Tabibzadehactivity assay,"Cayman Chemical, Ann Arbor, MI",701350 TET activity kit,"Epigentek, Farmingdale, NY",P-3086 XTT Assay,"Thermo Fisher Scientific, Waltham, MA",X12223 ATP assay,"Abcam, Waltham, MA",ab83355 NAD+

(4) Lysine vitcylation is a vitamin C-derived protein modification that enhances STAT1-mediated immune responseCellApril 3, 2025Xiadi He, Qiwei Wang, Xin Cheng, Weihua Wang, Yutong Li, Yabing Nan, Jiang Wu, Bingqiu Xiu, Tao Jiang, Johann S. Bergholz, et al.kit (abcam, ab139476), and epigenase 5mC-hydroxylase TET activity/inhibition assay kit (Epigentek, P-3086–48) were used for cellular vitC level assay, ROS level assay, and TET activity assay according to the

(5) Targeted Delivery of α‐ketoglutarate to Macrophages in Bone: A Novel Therapeutic Strategy for Improving Fracture Healing in Type 2 DiabetesAdvanced ScienceFebruary 25, 2025Jing Wang, Jiahao Cao, Siqi Zhang, Hongli Chen, Xuebing Yu, Xinli Wang, Tianji Wang, Wei Cao, Wengang Dong, Xinsen Lin, et al.EpiQuik Nuclear Extraction Kit I (#OP‐0002, EpigenTek) and Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (#P‐3086, EpigenTek) according to the manufacturer's instructions.[ 23 ] Briefly, to prepare nuclear extracts,

(6) Prenatal 1‐Nitropyrene Exposure Causes Autism‐Like Behavior Partially by Altering DNA Hydroxymethylation in Developing BrainAdvanced ScienceJuly 1, 2024Ting Zhao, Cheng‐Qing Huang, Yi‐Hao Zhang, Yan‐Yan Zhu, Xiao‐Xi Chen, Tao Wang, Jing Shao, Xiu‐Hong Meng, Yichao Huang, Hua Wang, et al.(78833) were from Thermo Fisher Scientific (USA). Ten eleven translocation (TET) enzymatic activity kit (P‐3086‐96) was from Epigentek (USA). All other regents were from Sigma Aldrich (Germany). 2.2 Animal Experiments All

(7) The regulation of plasma gelsolin by DNA methylation in ovarian cancer chemo-resistanceJournal of Ovarian ResearchJanuary 12, 2024Hafiza Bushra Manzoor, Meshach Asare-Werehene, Satyajit Dey Pereira, Kapaettu Satyamoorthy, Benjamin K. Tsang(Toronto, Canada). TET activity kit (Epigenase 5mC-Hydroxylase TET activity/Inhibition assay kit, Cat# P-3086) was purchased from EpigenTek Group Inc (New York, USA). TET1 (Cat#31,417), TET2 (Cat#31,418), and TET3

(8) DNA methylation changes underlie the long-term association between periodontitis and atherosclerotic cardiovascular diseaseFrontiers in Cardiovascular MedicineApril 21, 2023Mohamed Omar, Maria Alexiou, Umar R. Rekhi, Konrad Lehmann, Aneesh Bhardwaj, Cole Delyea, Shokrollah Elahi, Maria FebbraioTo determine TET activity, we used the Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (P-3086-96, Epigentek) and followed the manufacturer's protocol. BMDM nuclear extracts were used in this assay. S-adenosylmethionine

(9) MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylasesLeukemiaApril 1, 2022Lin AP, Qiu Z, Ethiraj P, Sasi B, Jaafar C, Rakheja D, Aguiar RCT.Activity Kit and the 5mC-Hydroxylase TET Activity Assay Kit, respectively (Epigentek, #P-9013 and #P-3086). Metabolite quantification by mass spectrometry. The measurements of the D-2-HG, L-2-HG and αKG were

(10) Regular Intake of Green Tea Polyphenols Suppresses the Development of Nonmelanoma Skin Cancer through miR-29-Mediated Epigenetic ModificationsJournal of clinical medicineJanuary 13, 2022Kansal V, Agarwal A, Harbour A, Farooqi H, Singh VK, Prasad R.ELISA assay kits for global DNA methylation (#P-1030), DNMT activity (#P-3009), and TET activity (#P-3086-96) were purchased from Epigentek, Inc. (Farmingdale, NY, USA). The secondary horseradish peroxidase

(11) Placental superoxide dismutase 3 mediates benefits of maternal exercise on offspring healthCell metabolismMay 4, 2021Kusuyama J, Alves-Wagner AB, Conlin RH, Makarewicz NS, Albertson BG, Prince NB, Kobayashi S, Kozuka C, Møller M, Bjerre M, et al.MyBioSource). TET1 activity was analyzed by Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (P-3086, EpiGentek). Plasma insulin were measured using Ultra Sensitive Mouse Insulin ELISA kits (90080, Crystal Chem Inc

(12) Acute high folic acid treatment in SH-SY5Y cells with and without MTHFR function leads to gene expression changes in epigenetic modifying enzymes, changes in epigenetic marks, and changes in dendritic spine densitiesPLOS ONEJanuary 7, 2021Daniel F. Clark, Rachael Schmelz, Nicole Rogers, Nuri E. Smith, Kimberly R. ShorterDNMTs (Cat. No. P-3009-48), HMTs (H3K4) (Cat. No. P-3002-1), HATs (Cat. No. P-4003-48), TETs (Cat. No. P-3086-48), and HDACs (Cat. No. P-4034-48) were used. Nuclear extracts were loaded in equal amounts into the

(13) Lung Allograft Epithelium DNA Methylation Age Is Associated With Graft Chronologic Age and Primary Graft DysfunctionFrontiers in immunologyJanuary 1, 2021Dugger DT, Calabrese DR, Gao Y, Deiter F, Tsao T, Maheshwari J, Hays SR, Leard L, Kleinhenz ME, Shah R, et al.methyltransferase) activity assay (Epigentek P-3009) or TET (ten-eleven translocation enzyme) activity assay (P-3086) following the manufacturer’s protocols to determine DNMT and TET activity with or without exposure

(14) The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic-epigenetic reprogramming and resolves inflammatory injuryNature immunologyNovember 1, 2020Zhou B, Magana L, Hong Z, Huang LS, Chakraborty S, Tsukasaki Y, Huang C, Wang L, Di A, Ganesh B, et al.activity were measured using the Epigenase™ 5mC-Hydroxylase TET Activity Assay Kit (Epigentek, Cat#P-3086) and Epigenase™ JMJD3 Demethylase Activity kit (Epigentek, Cat#P-3084) respectively according to the

(15) MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and EpitranscriptomeCell chemical biologyMay 21, 2020Qiu Z, Lin AP, Jiang S, Elkashef SM, Myers J, Srikantan S, Sasi B, Cao JZ, Godley LA, Rakheja D, et al.thiocyanate)." Critical Commercial Assays,, 5mC-Hydroxylase TET Activity Assay Kit,Epigentek,Cat#P-3086 m6A Demethylase Activity Kit,Epigentek,Cat#P-9013 Methylated DNA (5mC) Quantification Kit,Epigentek,Cat#P-1034 Hydroxymethylated

(16) TET2-interacting long noncoding RNA promotes active DNA demethylation of the MMP-9 promoter in diabetic wound healingCell Death & DiseaseNovember 1, 2019Liyan Zhou et al.DNMT was assessed in parallel using the Colorimetric TET or DNMT Activity/Inhibition Assay Kit or (P-3086, P-3009, Epigentek, Farmingdale, USA) according to manufacturers’ instructions. Briefly, 10 μg nuclear

(17) EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoterTheranosticsJanuary 1, 2019Shi F, Zhou M, Shang L, Du Q, Li Y, Xie L, Liu X, Tang M, Luo X, Fan J, et al.EpiQuik™ DNMT activity assay kit and Epigenase™ 5-mC hydroxylase TET activity assay kit (Cat: P3009 and P3086, EpigenTek, Farmingdale, NY, USA), respectively. FH activity was measured by a fumarase activity colorimetric

(18) Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb modelPhysiological genomicsOctober 1, 2018Ke X, Johnson H, Jing X, Michalkiewicz T, Huang YW, Lane RH, Konduri GG.dioxygenases was determined with the Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (cat. # P-3086; Epigentek, Farmingdale, NY). The assay is based on the conversion of methylated substrate present in

(19) Selective inhibition of CTCF binding by iAs directs TET-mediated reprogramming of 5-hydroxymethylation patterns in iAs-transformed cellsToxicology and applied pharmacologyJanuary 1, 2018Rea M, Gripshover T, Fondufe-Mittendorf Y.the Epigentek-Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) (Epigentek #P-3086). TET activity was calculated in two ways: 1) Specific Activity through a standard curve (expressed

(20) Oxidative stress alters global histone modification and DNA methylationFree radical biology & medicineMay 1, 2015Yingmei Niu, Thomas L DesMarais, Zhaohui Tong, Yixin Yao, Max Costa

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Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)

Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) from EpiGentek

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Epigenase 5mC Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) from EpiGentek

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