Description
The Quantikine HS Human IL-1ß Immunoassay is a 4 hour solid phase ELISA designed to measure human IL-1ß levels in serum, plasma, and saliva. It contains E. coli-expressed recombinant human IL-1ß and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1ß. Results obtained using natural IL-1ß showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural human IL-1ß. Reports indicate that ELISA kits calibrated using mature IL-1ß as a standard will detect, but considerably underestimate, the unprocessed IL-1ß precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1ß is usually not the predominant form of IL-1ß present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1ß present in serum, plasma, and saliva