Description
The Human Complement C1s ELISA Pair Set is for the quantitative determination of Human Complement C1s. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Complement C1s coated on a 96-well plate. Standards and samples are added to the wells, and any Human Complement C1s present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Complement C1s monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Complement C1s present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm