PD-1/PD-L1 Blockade Bioassay J1250 from Promega

Product Specs
ItemPD-1/PD-L1 Blockade Bioassay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ1250
Quantity1 each

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Simultaneous secretion of a PD-L1 x 4-1BB bispecific antibody improves antileukemic efficacy of STAb-T cells secreting a CD19-specific T-cell engagerOncoimmunologyDecember 31, 2025Marina Gómez-Rosel, Laura Rubio-Pérez, Ivana Zagorac, Carmen Domínguez-Alonso, Laura Díez-Alonso, Oana Hangiu, Rocío Navarro, Raúl Torres-Ruíz, Sandra Rodríguez-Perales, Francisco J. Blanco, et al.human PD-L1 (PD-L1 aAPC/CHO-K1), referred to as CHOAPC/PD-L1, were obtained from Promega (Cat. No. J1250, Promega, Madison, WI, USA). Jurkat T cells stably expressing human 4-1BB and NFAT-induced luciferase

(2) PD-L1 directed bispecific Vδ2-T cell engager combines lysis of PD-L1 expressing tumor cells with PD-1 immune checkpoint inhibition and modulation of the tumor immune microenvironmentJ Immunother CancerOctober 29, 2025Lisa A King, Myrthe Veth, Marieke Roos, George L Scheffer, Marko A Popovic, Tanja D de Gruijl, Hans J van der Vlietwas analyzed using flow cytometry. Second, the commercially available PD-1/PD-L1 blockade bioassay (J1250, Promega) was used. To do so, CHO-K1 cells (stably expressing PD-L1 and a cell surface protein designed

(3) Nanofilament immunotherapy induces potent antitumor vaccine responsesJournal for immunotherapy of cancerAugust 31, 2025Neil K, Génier S, Poisson MÈ, Douchin J, Giguère H, Daniel L, Gonzalez MF, Huang S, Trinh VQ, Tai LH, et al.specifications for each cell line. For the PD-1 blockade assay, we used Promega’s thaw-and-use kit (J1250) and strictly followed the manufacturer’s protocol. Luminescence readings were obtained using a Tecan

(4) The bispecific antibody targeting VISTA and PD-L1 shows enhanced tumor inhibitory activity in pancreatic, endometrial and breast cancers compared to mono- and combination immune checkpoint blockadeFront ImmunolMay 9, 2025Przemysław Bielski, Jan Barczyński, Michał Mikitiuk, Maja Myrcha, Kamil Rykała, Louis Boon, Wiktoria Gąsior, Aleksandra Hec-Gałązka, Tad A. Holak, Tomasz Sitarwas made by adding 0.5% Tween 20 (cat. M147, VWR) to the 1× PBS. PD-1/PD-L1 blockade bioassay (cat. J1250) was purchased from Promega (Madison, WI, USA). Design of antibody constructs Atezolizumab and onvatilimab

(5) NI-3201 Is a Bispecific Antibody Mediating PD-L1-Dependent CD28 Co-stimulation on T Cells for Enhanced Tumor ControlCancer immunology researchMarch 4, 2025Majocchi S, Lloveras P, Nouveau L, Legrand M, Viandier A, Malinge P, Charreton M, Raymond C, Pace EA, Millard BL, et al.S3. PD-1/PD-L1 blockade assay The PD-1/PD-L1 blockade reporter bioluminescent cell-based assay (Promega, Cat. #J1250) was used according to the manufacturer’s instructions to measure the potency of the mentioned antibodies

(6) CLIPA protein pairs function as cofactors for prophenoloxidase activation in Anopheles gambiaeInsect Biochemistry and Molecular BiologyFebruary 1, 2025Yang Wang, Qiao Jin, Michael R. Kanost, Haobo Jiangamplified from a larval A. gambiae cDNA sample using primer pairs j1245-j1246, j2147-j2148, and j2149-j1250 (Table S1), respectively. Major PCR products were cloned into pGEM-T vector (Promega). After sequence

(7) A yeast-based oral therapeutic delivers immune checkpoint inhibitors to reduce intestinal tumor burdenCell Chemical BiologyJanuary 16, 2025Olivia N. Rebeck, Miranda J. Wallace, Jerome Prusa, Jie Ning, Esse M. Evbuomwan, Sunaina Rengarajan, LeMoyne Habimana-Griffin, Suryang Kwak, David Zahrah, Jason Tung, et al.checkpoint blockade of microbially-derived ICI payloads is a commercially available assay (Promega, J1250) that relies on a luminescent reporter linked to PD-1/PD-L1 inhibition between two cell types. The cell

(8) Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developabilitymAbsDecember 31, 2024Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, et al.variant h1340.SA.LV, functional blocking assays were performed using the PD-1/PD-L1 Blockade Bioassay (J1250, Promega) following manufacturer’s instructions. Briefly, 4 × 104 CHO-K1 cells expressing hPD-L1 in00

(9) Percutaneous Delivery of Oncogel for Targeted Liver Tumor Ablation and Controlled Release of TherapeuticsAdvanced MaterialsNovember 1, 2024Hassan Albadawi, Zefu Zhang, Hyeongseop Keum, Enes Cevik, Bolni M Nagalo, Seyda Gunduz, Hirohito Kita, Rahmi Oklubioactivity of Nivo was evaluated using a PD-1/PD-L1 Blockade Bioassay following manufacturer instructions (J1250, Promega, Madison, WI). Samples, including OG hydrogel (3%NS and 25% IL) containing 1 mg/mL Nivo (OG+Nivo),

(10) Comparative Analytical Evaluation of the Proposed Biosimilar FYB206 and its Reference Medicinal Product Keytruda®Drugs in R&DSeptember 4, 2024Jakob C. Stüber, Kerstin Uhland, Alwin Reiter, Steffen Jakob, Florian Wolschinlines expressing PD-1 and PD-L1 using the Promega PD-1/PD-L1 blockade bioassay kit (catalogue numbers: J1250, J1255). The PD-L1 receptor of PD-L1 aAPC cells binds to the PD-1 receptor of PD-1 effector cells (engineered

(11) Molecular Engineering of Functional SiRNA AgentsACS Synthetic BiologyJune 21, 2024Neelu Batra, Mei-Juan Tu, Ai-Ming YuFBS, 200 μg/mL hygromycin B, and 200 μg/mL G418 sulfate solution. PD-1 effector cells (Promega, Cat #J1250) were cultured in 90% RPMI-1640, supplemented with 10% FBS, 200 μg/mL hygromycin B, 500 μg/mL G418 sulfate

(12) Multiparatopic antibodies induce targeted downregulation of programmed death-ligand 1Cell Chemical BiologyMay 16, 2024Seth D. Ludwig, Bunyarit Meksiriporn, Jiacheng Tan, Rakeeb Kureshi, Akhilesh Mishra, Kyle J. Kaeo, Angela Zhu, Georgia Stavrakis, Stephen J. Lee, David. J. Schodt, et al.Protein Labeling Kit,ThermoFisher Scientific,Cat# P36015 PD-1/PD-L1 Blockade Bioassay Kit,Promega,Cat# J1250 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines Freestyle™ 293-F cells

(13) Characterization of a Trispecific PD-L1 Blocking Antibody That Exhibits EGFR-Conditional 4-1BB Agonist ActivityAntibodies (Basel)April 24, 2024Laura Rubio-Pérez, Susana Frago, Marta Compte, Rocío Navarro, Seandean L. Harwood, Rodrigo Lázaro-Gorines, Marina Gómez-Rosel, Oana Hangiu, Noelia Silva-Pilipich, Lucía Vanrell, et al.stably expressing human PD-L1 (PD-L1 aAPC/CHO-K1) were obtained from Promega (Madison, WI, USA; cat# J1250). Jurkat T cells stably expressing human 4-1BB and NFAT-induced luciferase (JurkatNFAT-4-1BB) were obtained

(14) Effective cancer immunotherapy combining mRNA-encoded bispecific antibodies that induce polyclonal T cell engagement and PD-L1-dependent 4-1BB costimulationFrontiers in immunologyJanuary 1, 2024Hangiu O, Navarro R, Frago S, Rubio-Pérez L, Tapia-Galisteo A, Díez-Alonso L, Gómez-Rosel M, Silva-Pilipich N, Vanrell L, Smerdou C, et al.and CHO-K1 cells stably expressing human PD-L1 (PD-L1 aAPC/CHO-K1) were obtained from Promega (cat# J1250) and were used following the manufacturer’s instructions. Briefly, 0.3 × 105 PD-L1 aAPC/CHO-K1 cells/well

(15) A PD-L1/EGFR bispecific antibody combines immune checkpoint blockade and direct anti-cancer action for an enhanced anti-tumor responseOncoImmunologyDecember 31, 2023Laura Rubio-Pérez, Rodrigo Lázaro-Gorines, Seandean L. Harwood, Marta Compte, Rocío Navarro, Antonio Tapia-Galisteo, Jaume Bonet, Belén Blanco, Simon Lykkemark, Ángel Ramírez-Fernández, et al.and CHO-K1 cells stably expressing human PD-L1 (PD-L1 aAPC/CHO-K1), were obtained from Promega (cat# J1250). CHO-K1 cells stably expressing human PD-L1 (CHOPD-L1) were obtained from Genlantis (xCELLerateTM PD-L1

(16) In silico evolution of autoinhibitory domains for a PD-L1 antagonist using deep learning modelsProceedings of the National Academy of Sciences of the United States of AmericaDecember 5, 2023Goudy OJ, Nallathambi A, Kinjo T, Randolph NZ, Kuhlman B.PD-1:PD-L1 Blockade Bioassay. The activity of the masked antagonists was analyzed using a PD-1:PD-L1 Blockade Bioassay (Promega, J1250) according to a modified manufacturer’s protocol as described previously (31). Briefly, PD-L1 aAPC/CHO-K1 cells

(17) Design and selection of anti-PD-L1 single-domain antibody and tumor necrosis factor superfamily ligands for an optimal vectorization in an oncolytic virusFrontiers in Bioengineering and BiotechnologyNovember 15, 2023Christelle Remy, Elodie Pintado, Marshall Dunlop, Shirley Schön, Patricia Kleinpeter, Homa Rozanes, Laetitia Fend, Renée Brandely, Michel Geist, Delphine Suhner, et al.using GraphPad Prism software. PD1/PD-L1 blocking bioassay PD-1/PD-L1 blockade bioassay (Promega, J1250) was used to measure the potency of antibodies to block the cellular PD1/PDL1 interaction and following

(18) IGF2 Peptide-Based LYTACs for Targeted Degradation of Extracellular and Transmembrane ProteinsMolecules (Basel, Switzerland)November 10, 2023Mikitiuk M, Barczyński J, Bielski P, Arciniega M, Tyrcha U, Hec A, Lipińska AD, Rychłowski M, Holak TA, Sitar T.was made by adding 0.5% Tween 20 (cat. M147, VWR) to the 1× PBS. PD-1/PD-L1 blockade bioassay (cat. J1250) was purchased from Promega (Madison, WI, USA). 4.2. Design of IGF2-Based Polypeptides with Abrogated

(19) In silico evolution of protein binders with deep learning models for structure prediction and sequence designbioRxiv : the preprint server for biologyMay 3, 2023Goudy OJ, Nallathambi A, Kinjo T, Randolph N, Kuhlman B.PD-1:PD-L1 blockade bioassay The activity of the masked antagonists was analyzed using a PD-1/PD-L1 Blockade Bioassay (Promega, J1250) according to a modified manufacturer’s protocol as described previously(32). Briefly, PD-L1 aAPC/CHO-K1 cells

(20) A strategy for the efficient construction of anti-PD1-based bispecific antibodies with desired IgG-like propertiesmAbsDecember 31, 2022Jie Zhao et al.of PD1/PDL1 interaction was assessed using a commercially available bioassay system (Promega, Cat# J1250 and J1255) and following the manufacturer’s instruction. Briefly, PDL1/CHO (an artificial APC, or aAPC)

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PD-1/PD-L1 Blockade Bioassay

PD-1/PD-L1 Blockade Bioassay from Promega

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PD-1/PD-L1 Blockade Bioassay from Promega

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