pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector from Promega

Product Specs
ItempGL4.49[luc2P/TCF-LEF RE/Hygro] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4611
Quantity20microg
Molecular Weight~73kDa
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepGL4.49[luc2P/TCF-LEF RE/Hygro]

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Bioinformatics analysis of PSAT1 loss identifies downstream pathways regulated in EGFR mutant NSCLC and a selective gene signature for predicting the risk of relapseOncology LettersOctober 17, 2024Rumeysa Biyik‑Sit, Sabine Waigel, Kalina Andreeva, Eric Rouchka, Brian F. Clem2-oxoglutarate (cat. no. 349631) were obtained from MilliporeSigma. pGL4.49[luc2P/TCF-LEF/Hygro] Vector (E4611) and the Dual-Luciferase Reporter Assay System (cat. no. E1960) were purchased from Promega Corporation.

(2) Highly multiplexed bioactivity screening reveals human and microbiota metabolome-GPCRome interactionsCellJuly 1, 2023Haiwei Chen, Connor E. Rosen, Jaime A. González-Hernández, Deguang Song, Jan Potempa, Aaron M. Ring, Noah W. PalmRE-Luc2P,Promega,Cat#E8481 SRF-Luc2P,Promega,Cat#E1350 ISRE-Luc2P,Promega,Cat#E4141 NFκB-Luc2P,Promega,Cat#E8491 TCF-LEF-Luc2P,Promega,Cat#E4611 Gαs-Gαt and Gαs-Gαo Chimeras,Liberles et al.42,N/A pSin4-EF2-IRES-Pur,Addgene,Cat#16578 psPAX2,Addgene,Cat#12260 pMD2.G,Addgene,Cat#12259 pCER206

(3) Protocol for a Wnt reporter assay to measure its activity in human neural stem cells derived from induced pluripotent stem cellsCurr Res NeurobiolJanuary 1, 2023Yde Ohki CM, Walter NM, Rickli M, Salazar Campos JM, Werling AM, Döring C, Walitza S, Grünblatt E.for further transfections, the pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector (kindly provided by Promega AG, E4611) and an internal control vector under the control of a TK promoter (pNL1.1.TK [Nluc/TK] Vector; Promega

(4) Tumor-Intrinsic PD-L1 Exerts an Oncogenic Function through the Activation of the Wnt/β-Catenin Pathway in Human Non-Small Cell Lung CancerInternational journal of molecular sciencesSeptember 20, 2022Ma Y, Marinkova R, Nenkov M, Jin L, Huber O, Sonnemann J, Peca N, Gaßler N, Chen Y.co-transfected with pGL4.49[luc2P/TCF-LEF-RE/Hygro] (Promega; E6921) and pGL4.74 [hRluc/TK] (Promega; E4611) (ratio 10:1) using Lipofectamine®2000 transfection reagent (Invitrogen) following the manufacturer’s

(5) Endothelial Shp2 deficiency controls alternative activation of macrophage preventing radiation-induced lung injury through notch signalingiScienceMarch 18, 2022Liu P, Li Y, Li M, Zhou H, Zhang H, Zhang Y, Xu J, Xu Y, Zhang J, Xia B, et al.manuscript,N/A Mutant-β-catenin,Addgene,Cat. # 82176 pGL4.49(luc2P/TCF-LEF RE/Hygro),Promega,Cat. # E4611 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(6) Identification and Functional Analysis of a Novel CTNNB1 Mutation in Pediatric MedulloblastomaCancers (Basel)January 14, 2022Lide Alaña et al.Rockville, MD, USA), pGL4.27 luc2P (#E8451; Promega, Madison, WI, USA), and pGL4.49 luc2P TCF/LEF (#E4611; Promega). U2OS cells were transfected using Effectene (Qiagen) transfection reagent following manufacturer’s

(7) circNSUN2 promotes the malignant biological behavior of colorectal cancer cells via the miR‑181a‑5p/ROCK2 axisOncology reportsJuly 1, 2021Chi J, Liu S, Wu Z, Shi Y, Shi C, Zhang T, Xiong B, Zeng Y, Dong X.wild-type (WT) or mutant (MUT) binding sites of miR-181a-5p were cloned into pGL4.49 vector (cat. no. E4611; Promega Corporation). The 293T cells were plated and co-transfected with vectors by Lipofectamine 2000

(8) A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt BioavailabilityCell reportsOctober 9, 2018Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, Miao Y, Essler M, Noda M, Garcia KC, et al.paper,N/A p3xFLAG-CMV-9-Fzd8 (mouse),This paper,N/A pGL4.49[luc2P/TCF-LEF/Hygro] (STF reporter),Promega,Cat# E4611 pRL-TK (Renilla luciferase reporter),Promega,Cat# E2241 pLentiCRISPR,"Sanjana et al., 2014",Addgene

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