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Molecular Biology
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pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)
pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1) from Addgene Inc.
Be the first to write a review!
Citations:
(13)
Supplier Page
Supplier Page from
Addgene Inc. for
pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)
Description
Helper virus for monosynaptic tracing with rabies virus
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)
Company
Addgene Inc.
Price
Supplier Page
View Company Product Page
Catalog Number
52473-AAV1
Quantity
100 µL
Purity
suitable purity for injection into animals
Species
recombinant adeno-associated virus (rAAV)
Molecule Name
TVA-P2A-EGFP-P2A-B19G
Source/Expression System
Mammalian
Vector Name
pAAV-synP-FLEX-splitTVA-EGFP-B19G
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Addgene Inc.
490 Arsenal Way
Suite 100
Watertown
,
Massachusetts
02472
United States
Phone
: +1 (617) 233-4363
Email: help@addgene.org
Company Profile
Website:
www.addgene.org/
Citations (13)
(1)
Basal forebrain cholinergic input mediates adaptive attention allocation to enhance olfactory discrimination
PLOS Biology
September 16, 2025
Rahul Garg, Qiang Qiu, C. Ron Yu
tracing DAT-Cre animals were injected with
rabies
helper AAV (AAV1- synP-FLEX-splitTVA-EGFP-B19G,
Addgene
,
52473-AAV1
) to express glycoprotein (G) and the avian viral receptor TVA in SACs. At 3 weeks post AAV
injection
,
(2)
Organization of Brainwide Inputs to Discrete Lateral Septum Projection Populations
J Neurosci
September 10, 2025
Jennifer Isaac, Sonia C. Karkare, Hymavathy Balasubramanian, Malavika Murugan
AAV5-pCAG-FLEX-EGFP-WPRE (
51502-AAV5
), AAVrg-EF1a-mCherry-IRES-Cre (
55632-AAVrg
), AAV1-synP-FLEX-splitTVA-EGFP-B19G (
52473-AAV1
), and pENN-AAVrg-hSyn-Cre-WPRE-hGH(
105553-AAVrg
). The modified
rabies virus
,
Rabies
-N2C-deletedG-mCherry-EnvA,
(3)
Organization of Brainwide Inputs to Discrete Lateral Septum Projection Populations
J Neurosci
September 10, 2025
Jennifer Isaac, Sonia C. Karkare, Hymavathy Balasubramanian, Malavika Murugan
AAV5-pCAG-FLEX-EGFP-WPRE (
51502-AAV5
), AAVrg-EF1a-mCherry-IRES-Cre (
55632-AAVrg
), AAV1-synP-FLEX-splitTVA-EGFP-B19G (
52473-AAV1
), and pENN-AAVrg-hSyn-Cre-WPRE-hGH(
105553-AAVrg
). The modified
rabies virus
,
Rabies
-N2C-deletedG-mCherry-EnvA,
(4)
A ventral hippocampal-lateral septum pathway regulates social novelty preference
Elife
June 4, 2025
Maha Rashid, Sarah Thomas, Jennifer Isaac, Sonia Corbett Karkare, Hannah Klein, Malavika Murugan
A-C"
26966-AAV5
,v32533,AAV5-Ef1a-DIO-eNpHR3.0-EYFP,
Addgene
,1.1×10^13,2
51502-AAV5
,v60751,AAV5-CAG-FLEX-EGFP,Addgene,1.1×10^13,"2, 3 A-C"
52473-AAV1
,v14715,AAV1-synP-FLEX-TVA-EGFP-B19G,
Addgene
,2.4×10^13,"3 F-K, 5" ,SV-17–43,
Rabies
-G-deleted-N2C-mCherry
(5)
A ventral hippocampal-lateral septum pathway regulates social novelty preference
Elife
June 4, 2025
Maha Rashid, Sarah Thomas, Jennifer Isaac, Sonia Corbett Karkare, Hannah Klein, Malavika Murugan
A-C"
26966-AAV5
,v32533,AAV5-Ef1a-DIO-eNpHR3.0-EYFP,
Addgene
,1.1×10^13,2
51502-AAV5
,v60751,AAV5-CAG-FLEX-EGFP,Addgene,1.1×10^13,"2, 3 A-C"
52473-AAV1
,v14715,AAV1-synP-FLEX-TVA-EGFP-B19G,
Addgene
,2.4×10^13,"3 F-K, 5" ,SV-17–43,
Rabies
-G-deleted-N2C-mCherry
(6)
Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses
Nature Neuroscience
February 1, 2024
Lei Jin, Heather A. Sullivan, Mulangma Zhu, Thomas K. Lavin, Makoto Matsuyama, Xin Fu, Nicholas E. Lea, Ran Xu, YuanYuan Hou, Luca Rutigliani,
et al.
have been described previously19. These genomes were packaged in serotype 1 AAV capsids by
Addgene
(
52473-AAV1
,
100798-AAV1
and
100799-AAV1
). The two helper AAVs from
Addgene
were diluted in Dulbecco’s phosphate-buffered
(7)
Immediate responses to ambient light in vivo reveal distinct subpopulations of suprachiasmatic VIP neurons
iScience
October 20, 2023
Anat Kahan, Karan Mahe, Sayan Dutta, Pegah Kassraian, Alexander Wang, Viviana Gradinaru
strains
,Bacterial and
virus
strains
,Bacterial and
virus
strains
,, pAAV-synP-FLEX-splitTVA-EGFP-B19G,
Addgene
,
52473-AAV1
EnvA G-Deleted
Rabies
-mCherry,Salk,32636 ,, "Chemicals, peptides, and recombinant proteins","Chemicals,
(8)
Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network
Sci Adv
August 25, 2023
Qijun Tang, Elizabeth Godschall, Charles D. Brennan, Qi Zhang, Ruei-Jen Abraham-Fan, Sydney P. Williams, Taha Buğra Güngül, Roberta Onoharigho, Aleyna Buyukaksakal, Ricardo Salinas,
et al.
described. Retrograde tracing In
rabies virus
tracing, 200 nl of AAV1-synP-FLEX-splitTVA-EGFP-B19G (
Addgene
, #
52473-AAV1
) was injected to SCN of NMS-Cre
mice
using the stereotactic surgery method described above. Three weeks
(9)
A leptin-responsive hypothalamic circuit inputs to the circadian feeding network
bioRxiv
March 2, 2023
Qijun Tang, Elizabeth Godschall, Charles D. Brennan, Qi Zhang, Ruei-Jen Abraham-Fan, Sydney P. Williams, Taha Buğra Güngül, Roberta Onoharigho, Aleyna Buyukaksakal, Ricardo Salinas,
et al.
711-225-152
). Retrograde tracing
Rabies virus
tracing: 200nl AAV1-synP-FLEX-splitTVA-EGFP-B19G (
Addgene
#
52473-AAV1
) was injected to SCN of NMS-Cre
mice
using the stereotactic surgery method described above. Three weeks
(10)
Biased Connectivity of Brain-wide Inputs to Ventral Subiculum Output Neurons
Cell Reports
March 17, 2020
Ryan W.S. Wee, Andrew F. MacAskill
RRID:Addgene_105553 pAAV-synP-FLEX-splitTVA-EGFP-B19G,a gift from Ian Wickersham,
Addgene
viral prep #
52473-AAV1
; http://addgene.org/52473; RRID:Addgene_52473 RabiesΔG-EnvA-H2B-mCherry-2A-CLIP,"a gift from Marco
(11)
Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations
Frontiers in synaptic neuroscience
January 1, 2020
Lavin TK, Jin L, Lea NE, Wickersham IR.
packaged in serotype 1 AAV capsids by, and are available for purchase from,
Addgene
(catalog numbers
52473-AAV1
,
100798-AAV1
, and
100799-AAV1
). The titers of the AAVs, as determined by
Addgene
by
qPCR
, were as follows: AAV1-synP-FLEX-splitTVA-EGFP-B19G
(12)
Monosynaptic tracing: a step-by-step protocol
Journal of Chemical Neuroanatomy
December 1, 2019
Thomas K. Lavin, Lei Jin, Ian R. Wickersham
AAVs are available from
Addgene
packaged as serotype 1 (catalog numbers
100798-AAV1
,
100799-AAV1
, and
52473-AAV1
), and their genome plasmids are available from
Addgene
as well (catalog numbers 100798, 100799, and
(13)
Corticospinal Circuits from the Sensory and Motor Cortices Differentially Regulate Skilled Movements through Distinct Spinal Interneurons
Cell reports
May 1, 2018
Ueno M, Nakamura Y, Li J, Gu Z, Niehaus J, Maezawa M, Crone SA, Goulding M, Baccei ML, Yoshida Y.
Inst; Osakada et al., 2011)",Addgene #32636 AAV1-Syn-FLEX-TVA-2A-EGFP-2A-RVG,UNC vector core,
Addgene
#
52473 AAV1
-CAG-ChR2(H134R)-mCherry,Penn vector core,AV-1–20938M AAV8-hSyn-DIO-hM4D(Gi)-mCherry,UNC vector core,Addgene
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pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1) from Addgene Inc.
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
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