XbaI from New England Biolabs

Product Specs
ItemXbaI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0145T
Quantity3000 units
Concentration100,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

5'-T/CTAGA-3'

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Purification and characterization of recombinant human translation initiation factor eIF3Protein SciDecember 23, 2025Irene Diaz-Lopez, Yuliya Gordiyenko, Philipp K. Zuber, Xueyan Li, V. Ramakrishnancolonies after transformation were digested with BamHI and XbaI restriction enzymes (NEB, R3136M, and R0145M, respectively) for 1 h at 37°C. The digestion results in excision of each original gene expression cassette.

(2) Lymphodepleting chemotherapy potentiates neoantigen-directed T cell therapy by enhancing antigen presentationCell reports. MedicineDecember 16, 2025Sagie S, Babu T, Weller C, Tabachnik C, Livneh I, Quast NP, Gumpert N, Shomuradova A, Raybould MI, Levy R, et al.glucopyranoside,Sigma Aldrich,Cat#O8001 KAPA HiFi HotStart ReadyMix,Roche,Cat# KK2602 Not1,NEB,R0189S XBA1,NEB,R0145T CutSmart,NEB,#B6004 TurboFect,Thermo Fisher Scientific,Cat#R0531 DSP,Thermo Fisher Scientific,Cat#22585 L-cysteine,Sigma Aldrich

(3) Protocol to track single-cell-derived clones using DNA barcoding combined with single-cell RNA sequencingSTAR ProtocolsDecember 1, 2025Hee Jin Shin, Sahil Sharma, Sarah Kronheim, May Cheung, Long V. Nguyen880-545-CL Agarose D1-LE,WISENT INC,Cat # 800-015-EG XbaI digestion enzyme,New England Biolabs,Cat # R0145T SalI-HF digestion enzyme,New England Biolabs,Cat # R3138S 10X rCutSmart digestion buffer,New England Biolabs

(4) Evolution of the HIV-1 integration site landscape and inducible reservoir in early-treated peoplePLoS pathogensNovember 1, 2025Struyve T, Pardons M, De Clercq J, Termote L, Lambrechts L, Noppe Y, Lichterfeld M, Rutsaert S, Vandekerckhove L.Qiacuity probe master mix (Qiagen, #250102), 2 uL of each primer/probe set (S3 Table), 0.2 uL XbaI (NEB, #R0145M) and nuclease-free water to 40 uL total volume. Cycling was performed as follows: 2 min at 95°C; 40

(5) Radiotherapy‐resistant prostate cancer cells escape immune checkpoint blockade through the senescence‐related ataxia telangiectasia and Rad3‐related proteinCancer CommunicationsDecember 19, 2024Chenyi Shao, Yingyi Zhang, Hang Li, Jiajia Chen, Ting Huang, Jiaze Li, Simeng Wen, Sen Wang, Saijun Fan, Yu Zhaoinserted into the pcDNA3.1 vector with the T7 promoter. Plasmids were linearized by digestion with Xba I (R0145T, New England Biolabs) followed by purification with a TNT quick coupled transcription/translation system

(6) PXD101 inhibits malignant progression and radioresistance of glioblastoma by upregulating GADD45AJournal of translational medicineNovember 20, 2024Hu X, Zhou P, Peng X, Ouyang Y, Li D, Wu X, Yang L.Flag-HA-pcDNA3.1-vector plasmid was digested using Hind III-HF (R3104T, New England Biolabs, MA) and Xba I (R0145T, New England Biolabs), the GADD45A coding sequence was inserted into the vector to obtain the recombinant

(7) Strand asymmetry influences mismatch resolution during a single-strand annealingGenome BiologyDecember 1, 2022Victoria O. Pokusaeva, ArÃ¡nzazu Rosado Diez, Lorena Espinar, Albert TorellÃ³ PÃ©rez, Guillaume J. Filionwith DSB induction and control without the induction) was digested using 1 μl 100 U/μL XbaI (NEB, #R0145T) and 4 μl 5 U/μL I-SceI (NEB, #R0694L) in a 50-μL final volume for 3 h at 37 °C. Digested DNA was column-purified

(8) Potent monoclonal antibodies neutralize Omicron sublineages and other SARS-CoV-2 variantsCell ReportsNovember 1, 2022Zhaochun Chen, Peng Zhang, Yumiko Matsuoka, Yaroslav Tsybovsky, Kamille West, Celia Santos, Lisa F. Boyd, Hanh Nguyen, Anna Pomerenke, Tyler Stephens, et al.,New England BioLabs,R0146M SpeI-HF,New England BioLabs,R3133M SacI-HF,New England BioLabs,R3156M XbaI,New England BioLabs,R0145M NheI-HF,New England BioLabs,R3131S NotI,New England BioLabs,R3189S ApaI,New England BioLabs,R0114S AgeI,New England BioLabs

(9) Prime editor-mediated correction of a pathogenic mutation in purebred dogsScientific ReportsJuly 28, 2022Dong Ern Kim, Ji Hye Lee, Kuk Bin Ji, Eun Ji Lee, Chuang Li, Hyun Ju Oh, Kang Sun Park, Seung Hoon Lee, Okjae Koo, Min Kyu Kim5′-tctagaaatttcgataagccagtaagc-3′, and inserted into the vector by EcoRI and XbaI (NEB Inc., MA, USA: #R0101M and #R0145M) enzyme cuts. The pegRNA targeting the HD locus was newly synthesized and then added to the vector using

(10) Spike residue 403 affects binding of coronavirus spikes to human ACE2Nature communicationsNovember 25, 2021Zech F, Schniertshauer D, Jung C, Herrmann A, Cordsmeier A, Xie Q, Nchioua R, Prelli Bozzo C, Volcic M, Koepke L, et al.

(11) Optimized protocols for ChIP-seq and deletion mutant construction in Pseudomonas syringaeSTAR protocolsSeptember 17, 2021Yao C, Shao X, Li J, Deng X.peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, XbaI,NEB,Catalog # R0145M EcoRI-HF,NEB,Catalog # R3101M HindIII-HF,NEB,Catalog # R3104M T4 DNA Ligase,Takara,Catalog # 2011A Taq

(12) Dyskerin Mutations Present in Dyskeratosis Congenita Patients Increase Oxidative Stress and DNA Damage Signalling in Dictyostelium DiscoideumCellsNovember 8, 2019Rodriguez-Centeno J, Perona R, Sastre L.

(13) Structural Basis of the Differential Binding of Engineered Knottins to Integrins αVβ3 and α5β1StructureSeptember 1, 2019Johannes F. Van Agthoven et al.,New England Biolabs,R0136M EcoRI,New England Biolabs,R0101M BssHII,New England Biolabs,R0199M XbaI,New England Biolabs,R0145M Penicillin-Streptomycin,ThermoFisher,15140122 PBS,Boston BioProducts,Cat#BM-220 Hepes,Boston BioProducts,Cat#BBH-85 Alexa

(14) Robust Wnt signaling is maintained by a Wg protein gradient and Fz2 receptor activity in the developing Drosophila wingDevelopmentAugust 9, 2019Varun Chaudhary, Swapnil Hingole, Jana Frei, Fillip Port, David Strutt, Michael Boutros

(15) Mutant CEBPA directly drives the expression of the targetable tumor-promoting factor CD73 in AMLScience AdvancesJuly 10, 2019Janus S. Jakobsen et al.followed by a 1-hour incubation with 1% Triton X-100 at 37°C. Overnight digestion with 200 U of Xba I (R0145T, NEB) with the addition of 100 U of enzyme for the last 4 hours was followed by 30 min of heat inactivation

(16) Yeast Sirtuin Family Members Maintain Transcription Homeostasis to Ensure Genome StabilityCell ReportsJune 4, 2019Jessica L. Feldman, Craig L. Peterson20T,Seikagaku Biobusiness,Cat#120491 HindIII-HF,NEB,Cat#R3104M EcoRI-HF,NEB,Cat#R310M XbaI,NEB,Cat#R0145M SspI-HF,NEB,Cat#R3132M BsrG1-HF,NEB,Cat#R3575L NEB Buffer 2.1,NEB,Cat#B7202S RNase H,NEB,Cat#M0297L Proteinase

(17) Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi ArabiaTURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCESJanuary 1, 2017Maged Gomaa HEMIDA, Mohamed Al HAMMADIBamHI, CLaI, ECoRI, KpnI, and XbaI) (New England Biolabs, Cat Nos (R0114S, R0136S, R0197S, R0101S, R0142S, and R0145T, respectively) were used for the digestion of the extracted FAdV-4 DNAs. The digestion was carried out as

(18) Continuous directed evolution of DNA-binding proteins to improve TALEN specificityNature MethodsOctober 1, 2015Basil P. Hubbard, Ahmed H. Badran, John A. Zuris, John P. Guilinger, Kevin M. Davis, Liwei Chen, Shengdar Q. Tsai, Jeffry D. Sander, J. Keith Joung, David R. Liu

(19) MicroRNA 648 Targets ET-1 mRNA and is cotranscriptionally regulated with MICAL3 by PAX5Molecular and cellular biologyFebruary 1, 2015Li C, Gonsalves CS, Eiymo Mwa Mpollo MS, Malik P, Tahara SM, Kalra VK.

(20) Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 GenerationPLoS ONESeptember 25, 2014Rolf B. Edvardsen, Sven Leininger, Lene Kleppe, Kai Ove Skaftnesmo, Anna Wargelius

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(1)

9/11/2015Good XbaI Restriction Enzyme J GThis is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.

XbaI

XbaI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (21)

Reviews

XbaI from New England Biolabs

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