XbaI from New England Biolabs

Product Specs
ItemXbaI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0145S
Quantity3000 units
Concentration20-100 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

T/CTAGA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The dual specificity phosphatase 2 act as distal regulatory node in T cell signalingiScienceAugust 21, 2026Henrike Bruckmueller, Anangi Balasiddaiah, Sofia Malek, Victoria Tenhaken, Julien Bruckmueller, Jakob Mejlvang, Helene Spangenberg, Farah Syed, Bjarne Johansen, Hanne Kildalsen, et al.,New England Biolabs,R0101S Kpn1-HF,New England Biolabs,R3142S XhoI,New England Biolabs,R0146S XbaI,New England Biolabs,R0145S GST-ERK1,MRCPPU Reagents,DU1509 His-ERK2,Novus Biologicals,NBP1-30309 GST-p-ERK2,MRCPPU Reagents,DU650 GST-p38 alpha

(2) Genome instability is increased during DNA replication in the presence of the CPD UV photoproduct in transcriptionally active genesiScienceJuly 17, 2026Rika Kusumoto-Matsuo, Yusuke Matsuno, Naoki Akiba, Yuya Manaka, Jiro Toshima, Fumio Hanaoka, Ken-Ichi YoshiokaNew England Biolabs), 1 μL of SspI-HF (20 U/μL) (R3132S, New England Biolabs), and 1 μL of XbaI (20 U/μL) (R0145S, New England Biolabs). Fragmented DNA was purified with a QIAquick PCR Purification Kit (28104, QIAGEN).

(3) Regulatory changes underlying the evolution of skylight navigationiScienceJuly 17, 2026Heidi Roth, Melanie Sarfert, Aleksandra Simdianova, Jana Balke, Michael W. Perry, Katja Nowick, Mathias F. WernetDistilled Water,InvitrogenTM,10977015 VECTORSHIELD,Vector Laboratories,H-1000 XbaI,New England Biolabs,R0145S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, PureLinkTM

(4) Wiz regulates clustered protocadherin genes by restricting CTCF/cohesin loop extrusion in a genomic-distance biased mannerPLoS GenetJuly 16, 2026Tianjie Li, Jingwei Li, Leyang Wang, Haiyan Huang, Qiang WupcDNA3.1-Cas9 vector, containing Cas9 coding sequence under a T7 promoter, was linearized with XbaI (NEB, R0145S), purified by gel extraction, extracted with phenol-chloroform, and precipitated by ethanol. After ethanol

(5) Loss of heterozygosity exposes germline mutations in complex I and drives Warburg metabolism in oncocytic carcinoma of the thyroidSci AdvJuly 3, 2026Celia de la Calle Arregui, Anderson R. Frank, Kelvin Mun, Jiwoong Kim, Kuntal Majmudar, Justin A. Bishop, Cheryl Lewis, Yang Xie, David G. McFaddenPGK-IRES-Blast lentiviral vector previously digested with BamHI and XBaI (New England Biolabs, #R3136S and #R0145S) using Gibson Assembly (New England Biolabs, #E2621S), generating the final expression constructs. The

(6) Spatially resolved profiling of steroid nuclear receptors reveals a role for the disordered N-terminal domains in genome targeting and AP-1 interactionGenome ResearchJuly 1, 2026Inbal Zigdon, Achinoam Shoham, Vladimir Mindel, Wajd Manadre, David Jan, Beniamin Krupkin, Dekel Yahav Har-Shai, Yaron Antebi, Naama BarkaiSupplemental Tables 1–4), using restriction–ligation with XhoI and XbaI restriction enzymes (NEB R0146S, R0145S). Cell culture maintenance All mammalian cell lines (NMuMG and HepG2) were grown in Dulbecco's Modified

(7) STREMI: a dual-function upstream ORF-encoded regulator of mitochondrial cristae architectureEMBO ReportsMay 2, 2026Ruiyang Guo, Yabo Guo, Runguo Shu, Jiajia Qian, Jiawei Wang, Ruobing Li, Ti Qin, Ziyi Wang, Hongtao Tian, Mengchen Wu, et al.restriction enzyme,NEB,Cat#R3733V BbsI-HF restriction enzyme,NEB,Cat#R3539S XbaI restriction enzyme,NEB,Cat#R0145S ClaI restriction enzyme,NEB,Cat#R0197S AvrII restriction enzyme,NEB,Cat#R0174S MluI-HF restriction

(8) Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20International journal of molecular sciencesApril 29, 2026Corsi A, Valentino A, Bruno MG, Menichetti G, Belpinati F, Pereira MP, Valenti MT, Ruggiero A, Trabetti E, Bombieri C, et al.28704, Qiagen, Venlo, The Netherlands), and the RHCglo vector was enzymatically cut using XbaI (cat R0145S, New England Biolabs, Ipswich, MA, USA) and SalI High Fidelity® (cat R3138S, New England Biolabs, Ipswich,

(9) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.min → 95°C, 50 min → ramp down to 25°C at 5°C/min. XbaI or Bbs1 digestion (New England Biolabs Cat# R0145S or R0539S), DNA gel extraction (QIAGEN Cat# 28704), and ligation (New England Biolabs Cat# M0202S) were

(10) Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ traffickingNature CommunicationsApril 20, 2026Justin A. Bosch, Pierre Michel Jean Beltran, Cooper Cavers, James Thai LaGraff, Randy Melanson, Ankita Singh, Weihang Chen, Yanhui Hu, Sudhir Gopal Tattikota, Ying Liu, et al.Supplementary Data 8a. pMT-EGFP-V5 (Addgene #240240, DGRC #1696) was generated by EcoRI/XbaI (NEB, R3101S, R0145S) restriction enzyme digestion and ligation of PCR-amplified EGFP sequence into pMT/V5-His B (Invitrogen,

(11) HAMMER: hairpin-based APOBEC3A-mediated mRNA editing reporterNucleic Acids ResApril 2, 2026Yanjun Chen, Christopher D Mullally, Bojana Stefanovska, Reuben S HarrisHairpin1 reporter linker region with HindIII (New England Biolabs, R3104L) and XbaI (New England Biolabs, R0145L) and inserting gBlocks containing candidate A3A hairpin substrates (gBlock sequences in Supplementary

(12) Epigenetic memory of colitis promotes tumour growthNatureMarch 25, 2026Surya Nagaraja, Lety Ojeda-Miron, Ruochi Zhang, Ena Oreskovic, Conrad Hock, Yan Hu, Daniel Zeve, Karina Sharma, Roni R. Hyman, Qiming Zhang, et al.empty barcode vector (2 μg) was digested and dephosphorylated with BamHI (NEB, R3136T), XbaI (NEB, R0145S) and FastAP (Thermo, EF0652) for 4–12 h at 37 °C. Following purification, 1 μg of digested plasmid and

(13) Disease-causing mutations in Synaptotagmin can act via dominant-negative, gain-of-function or haploinsufficient mechanismsbioRxivMarch 19, 2026Zhuo Guan, Maria Bykhovskaia, J. Troy LittletonQuikChange Lightning Site-Directed Mutagenesis Kit,Agilent,Cat# 210518 EcoRI-HF,New England BioLabs,Cat# R3101S XbaI,New England BioLabs,Cat# R0145S Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(14) Characterization of HER2-Positive Murine Breast Cancer Models for Investigating HER2-Targeted Therapy and ImmunotherapyCancers (Basel)March 19, 2026Yun Lu, Benjamin P. Lee, Abbigael V. Eli, Shannon E. Lynch, Ar Rafi Md Faisal, Jonathan Moye, Anna G. Soraceplasmid (Addgene, #73582) and the PCR-amplified HER2WT or HER2YVMA insert were digested with XbaI (NEB, R0145L) and subsequently ligated using T4 DNA ligase (NEB, M0202S). The resulting plasmid maps are included

(15) Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecovirusesScientific ReportsMarch 18, 2026John A. Frank, Eric Xu Gan, William Brenham Hooper, Isabel Madeleine Ott, Akiko IwasakipCAGGS-BANAL52-S (MZ937000) were synthesized as gene blocks by Twist Bioscience and subcloned into XbaI (NEB R0145S) and NotI (NEB R3189S) double-digested pCAGGS plasmids by Gibson Assembly (NEB HiFi M5520). pTwist-SARS-CoV-2-BA1-S

(16) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie Pylesequences as described above were linearized with Xba1 (WT, SLII and DBI mutants, 3’-Terminus) (NEB, cat #R0145S) and EcoRI (SLIV and DBII mutants) (NEB, cat #R3101S). The linearized plasmid served as a template for

(17) DPEP2 suppresses hyperinflammation via metabolic reprogramming of macrophages in sepsisNature CommunicationsMarch 9, 2026Wenchen Luo, Wei Xu, Qimeng Yin, Jieqiong Song, Jie Wang, Yian Guan, Jian Huang, Jian Yu, Kefeng Zou, Danfeng Jin, et al.fragment and the pLVX-puro lentiviral expression vector78 (Addgene, 125839) were digested with XbaI (R0145S, New England Biolabs) and XhoI (R0146S, New England Biolabs), purified, and ligated using T4 DNA ligase.

(18) Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cellsScientific ReportsFebruary 20, 2026Nicole M. Jenkinson, Harsh Oza, Kevin J. Yarema, Kristine GlundeCampeau & Paul Kaufman, Addgene #17486) using restriction enzyme cloning with XbaI (New England Biolabs #R0145S) and SalI-HF (New England Biolabs #R3138S) using NEB rCutSmart Buffer (New England Biolabs #B6004S).

(19) Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytesSignal Transduction and Targeted TherapyFebruary 18, 2026Ziyin Zhang, Nanshu Xiang, Qian Liu, Yanfeng Li, Linhua Wang, Xinpu Yang, Ju Liu, Lichen Zhang, Liaoxun Lu, Yinming Liang, et al.were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by T4 ligation (Takara, 2011B). The plasmids expressing V5-tagged mouse RNF213

(20) HAMMER: Hairpin-based APOBEC3A-mediated mRNA editing reporterbioRxivFebruary 15, 2026Yanjun Chen, Christopher D. Mullally, Bojana Stefanovska, Reuben S. HarrisHairpin1 reporter linker region with HindIII (New England Biolabs, R3104L) and XbaI (New England Biolabs, R0145L) and inserting gBlocks containing candidate A3A hairpin substrates (gBlock sequences in Table S1). Stop1

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(1)

9/11/2015Good XbaI Restriction Enzyme J GThis is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.

XbaI

XbaI from New England Biolabs

Sequence

New England Biolabs

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Reviews

XbaI from New England Biolabs

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