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XbaI
XbaI
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XbaI
XbaI from New England Biolabs
(1)
Write a review
Citations:
(296)
$317.00 Inquire
from
New England Biolabs for
XbaI
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
XbaI
Company
New England Biolabs
Price
$317.00 Inquire
Catalog Number
R0145L
Concentration
20-100 u/µL
Quantity
15000 units
Applications
DNA Manipulation
Type
Restriction Enzyme
Temperature
-20 C
Sequence
T/CTAGA
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (296)
(1)
A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targets
Hemasphere
April 23, 2026
Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson,
et al.
min → 95°C, 50 min → ramp down to 25°C at 5°C/min.
XbaI
or Bbs1 digestion (
New England Biolabs
Cat#
R0145S
or
R0539S
), DNA gel extraction (
QIAGEN
Cat#
28704
), and
ligation
(
New England Biolabs
Cat#
M0202S
) were
(2)
HAMMER: hairpin-based APOBEC3A-mediated mRNA editing reporter
Nucleic Acids Res
April 2, 2026
Yanjun Chen, Christopher D Mullally, Bojana Stefanovska, Reuben S Harris
Hairpin1 reporter linker region with
HindIII
(
New England Biolabs
,
R3104L
) and
XbaI
(
New England Biolabs
,
R0145L
) and inserting gBlocks containing candidate A3A hairpin substrates (gBlock sequences in Supplementary
(3)
Epigenetic memory of colitis promotes tumour growth
Nature
March 25, 2026
Surya Nagaraja, Lety Ojeda-Miron, Ruochi Zhang, Ena Oreskovic, Conrad Hock, Yan Hu, Daniel Zeve, Karina Sharma, Roni R. Hyman, Qiming Zhang,
et al.
empty barcode vector (2 μg) was digested and dephosphorylated with
BamHI
(
NEB
,
R3136T
),
XbaI
(
NEB
,
R0145S
) and FastAP (
Thermo
,
EF0652
) for 4–12 h at 37 °C. Following
purification
, 1 μg of digested plasmid and
(4)
Characterization of HER2-Positive Murine Breast Cancer Models for Investigating HER2-Targeted Therapy and Immunotherapy
Cancers (Basel)
March 19, 2026
Yun Lu, Benjamin P. Lee, Abbigael V. Eli, Shannon E. Lynch, Ar Rafi Md Faisal, Jonathan Moye, Anna G. Sorace
plasmid (Addgene, #73582) and the
PCR
-amplified HER2WT or HER2YVMA insert were digested with
XbaI
(
NEB
,
R0145L
) and subsequently ligated using
T4 DNA ligase
(
NEB
,
M0202S
). The resulting plasmid maps are included
(5)
Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecoviruses
Scientific Reports
March 18, 2026
John A. Frank, Eric Xu Gan, William Brenham Hooper, Isabel Madeleine Ott, Akiko Iwasaki
pCAGGS-BANAL52-S (MZ937000) were synthesized as gene blocks by Twist Bioscience and subcloned into
XbaI
(
NEB
R0145S
) and
NotI
(
NEB
R3189S
) double-digested pCAGGS plasmids by Gibson Assembly (NEB HiFi M5520). pTwist-
SARS-CoV-2
-BA1-S
(6)
Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growth
bioRxiv
March 14, 2026
Lucille H. Tsao, Doug E. Brackney, Anna Marie Pyle
sequences as described above were linearized with Xba1 (WT, SLII and DBI mutants, 3’-Terminus) (
NEB
, cat #
R0145S
) and
EcoRI
(SLIV and DBII mutants) (
NEB
, cat #
R3101S
). The linearized plasmid served as a template for
(7)
DPEP2 suppresses hyperinflammation via metabolic reprogramming of macrophages in sepsis
Nature Communications
March 9, 2026
Wenchen Luo, Wei Xu, Qimeng Yin, Jieqiong Song, Jie Wang, Yian Guan, Jian Huang, Jian Yu, Kefeng Zou, Danfeng Jin,
et al.
fragment and the pLVX-puro lentiviral expression vector78 (Addgene, 125839) were digested with
XbaI
(
R0145S
,
New England Biolabs
) and
XhoI
(
R0146S
,
New England Biolabs
), purified, and ligated using T4 DNA ligase.
(8)
Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cells
Scientific Reports
February 20, 2026
Nicole M. Jenkinson, Harsh Oza, Kevin J. Yarema, Kristine Glunde
Campeau & Paul Kaufman, Addgene #17486) using restriction enzyme
cloning
with
XbaI
(
New England Biolabs
#
R0145S
) and
SalI-HF
(
New England Biolabs
#
R3138S
) using NEB
rCutSmart Buffer
(
New England Biolabs
#
B6004S
).
(9)
Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes
Signal Transduction and Targeted Therapy
February 18, 2026
Ziyin Zhang, Nanshu Xiang, Qian Liu, Yanfeng Li, Linhua Wang, Xinpu Yang, Ju Liu, Lichen Zhang, Liaoxun Lu, Yinming Liang,
et al.
were subsequently cloned and inserted into the pcDNA3.1 vector by
HindIII
(
NEB
,
R3104S
) and
XbaI
(
NEB
,
R0145S
) dual digestion, followed by T4
ligation
(Takara, 2011B). The plasmids expressing V5-tagged
mouse
RNF213
(10)
HAMMER: Hairpin-based APOBEC3A-mediated mRNA editing reporter
bioRxiv
February 15, 2026
Yanjun Chen, Christopher D. Mullally, Bojana Stefanovska, Reuben S. Harris
Hairpin1 reporter linker region with
HindIII
(
New England Biolabs
,
R3104L
) and
XbaI
(
New England Biolabs
,
R0145L
) and inserting gBlocks containing candidate A3A hairpin substrates (gBlock sequences in Table S1). Stop1
(11)
Integrase-On-Demand: bioprospecting integrases for targeted genomic insertion of genetic cargo
Nucleic Acids Res
February 9, 2026
Hannah M McClain, Lillian C Lowrey, Laura B Quinto, Ellis L Torrance, Tomas R Gagliano, Farren J Isaacs, Joseph S Schoeniger, Kelly P Williams, Catherine M Mageeney
into pUCP22 using restriction
cloning
(
Eco53kI
(
NEB
, Ipswich, MA,
R0116S
),
XbaI
(
NEB
, Ipswich, MA,
R0145S
),
Monarch® Spin PCR & DNA Cleanup Kit
(
NEB
, Ipswich, MA, T1130L), Ligase (
NEB
, Ipswich, MA,
M0202L
).
(12)
Cancer cachexia in STK11/LKB1-mutated non-small cell lung cancer is dependent on tumor-secreted GDF15
Nature Communications
January 30, 2026
Jinhai Yu, Tong Guo, Arun Gupta, Ernesto M. Llano, Thomas Salisbury, Naureen Wajahat, Dianne Zhao, Sean Slater, Qing Deng, Esra A. Akbay,
et al.
pLVX-EF1α-IRES-Hygro vector (kindly gifted by Shimeng Xu, UTSW Medical Center) by first treating with the
XbaI
(
NEB
, #
R0145S
) and BamH1-HF (NEB, #
R3136S
) restriction enzymes followed by In-Fusion Snap Assembly (Takara, #638947)
(13)
sRNA196 promotes Pseudomonas aeruginosa virulence by regulating target genes PA4398, ZapE and PhoB
Microbial Cell Factories
January 27, 2026
Fanghong Wang, Peng Ma, Peng Zhou, Yanmei Liu, Qiwei Li, Ling Zhang, Youqiang Li, Weiguo Yin, Juanjuan Han
fragment. Additionally, the suicide plasmid PGSM was double-digested with
SacI
(
NEB
, R3156) and
XbaI
(
NEB
,
R0145L
), and the purified product was recovered. Next,
seamless cloning
technology was used to insert the target
(14)
A prophage-expressed type IV pilus component provides anti-phage defense
Cell Reports
January 27, 2026
Kristina M. Sztanko, Taylor J. Ellison, Jill Greenlaw, Tatiana Lenskaia, P. Nathael Javorčík, Alexa D. Fitzpatrick, Karen L. Maxwell, Courtney K. Ellison, Alan R. Davidson
,
New England Biolabs
,Cat#
R3104S
KpnI-HF
,
New England Biolabs
,Cat#
R3142S
XbaI
-HF,
New England Biolabs
,Cat#
R0145S
BsaI-HF
v2,
New England Biolabs
,Cat#
R3733S
T4 DNA Ligase
,
New England Biolabs
,Cat#
M0202S
T4 Polynucleotide Kinase
(15)
Notch Signalling Plays a Role in Patterning the Ventral Mesoderm During Early Embryogenesis in Drosophila melanogaster
International Journal of Molecular Sciences
January 27, 2026
Marvel Megaly, Gregory Foran, Arsala Ali, Anel Turgambayeva, Tai Sengsouriya, Samantha Berube, Ryan Douglas Hallam, Ping Liang, Aleksandar Necakov
USA) by cutting the cassette out of pUASP-attb-Cry2-CTEV-mCherry using
NotI
and
XbaI
(
NEB
#
R3189S
and #
R0145S
, Whitby, ON, Canada) and ligating it to the linearized pAc5.1/V5-His A. The resultant plasmid was named
(16)
Construction and Biological Characterization of ORF133-Deletion Mutant of Lumpy Skin Disease Virus
Viruses
January 24, 2026
Qunhua Ke, Miaomiao Li, Yao Peng, Xiangwei Wang, Yuefeng Sun, Xiangping Yin, Yanming Wei
Buffer,P0013C
HindIII-HF
®,
R3104S
,
Neb
,Ipswich/MA/USA Neb,Ipswich/MA/USA,
EcoRI-HF
®,
R3101T
Neb
,Ipswich/MA/USA,
KpnI-HF
®,
R3142S
Neb
,Ipswich/MA/USA,
XbaI
,
R0145S
Neb
,Ipswich/MA/USA,
T4 DNA Ligase
,
M0202S
WestemBright ECL,
K-12045-D50
,
Advansta
,Menlo Park/CA/USA Main
(17)
Workflow for Gene Overexpression and Phenotypic Characterisation in Taraxacum kok-saghyz
Methods and Protocols
January 24, 2026
Loredana Lopez, Michele Antonio Savoia, Loretta Daddiego, Paolo Facella, Elio Fantini, Linda Bianco, Simone Maci, Francesco Panara
Darmstadt, Germany; Cat. no.: P1379).
XbaI
and
SacI
(
New England Biolabs
, Ipswich, MA, USA; Cat. no.:
R0145L
and
R3156L
). 2.2. Equipment Standard laboratory equipment: analytical balance; autoclave; electroporator
(18)
Loss of ATM causes R-loop-associated transcriptional dysregulation and attenuates the related response to DNA damage
Journal of Biological Chemistry
January 14, 2026
Katherine R. Westover, Yingzi Hou, Feng Wang, Yilin Wang, Yangping Li, Rachel Seong, Jie Xu, Zhexing Wen, Bing Yao
(
BsrGI
,
EcoRI
,
HindIII
,
SspI
, and
XbaI
, 30 units each) (
NEB
, #3575S, #
R3101S
, #
R3104S
, #
R3132L
, and #
R0145S
) at 37 °C overnight. The digested nucleic acids were then recovered by phenol–chloroform extraction.
Dot blots
(19)
Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosis
Nucleic Acids Research
January 8, 2026
Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh,
et al.
kb 66% AT + PciI_RP by
PCR
(Supplementary Table S5). Amplified fragment was digested with
XbaI
(
NEB
R0145S
) and
PciI
(
NEB
R0655S
) restriction enzymes and ligated to the already digested 18-kb plasmid with the
(20)
Global Evaluation of Congenital Heart Disease-Associated Non-Coding Variants
Research Square
January 7, 2026
Edwin G. Rodríguez-Martínez, Shreya Peña-Martínez, Joshua G. Medina-Feliciano, Joshua Medina-Feliciano, Lois G. Parks, Marissa Granitto, Diego A. Pomales-Matos, Jean L. Pomales-Matos, Jean Messon- Barreiro-Rosario, Adriana Barreiro-Rosario,
et al.
Aldrich,276855
NEBNext® Ultra™ II Q5® Master Mix
,
NEB
,
M0544S
Gibson Assembly Master Mix
,
NEB
,
E2611L
XbaI
,
NEB
,
R0145S
NcoI
,
NEB
,
R0193S
RecBCD,
NEB
,
M0345S
AsiSI
,
NEB
,R063L NEBNext
Q5® High-Fidelity 2X Master Mix
,
NEB
,
M0492L
Penicilin-Streptomycin,
ThermoFisher
,
15140122
SDS,Sigma
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Reviews
Write a Review
(1)
9/11/2015
Good XbaI Restriction Enzyme
J G
This is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.
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XbaI from New England Biolabs
Price
$317.00
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Product Specs
Company Info
Citations
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