XbaI from New England Biolabs

Product Specs
ItemXbaI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0145L
Concentration20-100 u/µL
Quantity15000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

T/CTAGA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.min → 95°C, 50 min → ramp down to 25°C at 5°C/min. XbaI or Bbs1 digestion (New England Biolabs Cat# R0145S or R0539S), DNA gel extraction (QIAGEN Cat# 28704), and ligation (New England Biolabs Cat# M0202S) were

(2) HAMMER: hairpin-based APOBEC3A-mediated mRNA editing reporterNucleic Acids ResApril 2, 2026Yanjun Chen, Christopher D Mullally, Bojana Stefanovska, Reuben S HarrisHairpin1 reporter linker region with HindIII (New England Biolabs, R3104L) and XbaI (New England Biolabs, R0145L) and inserting gBlocks containing candidate A3A hairpin substrates (gBlock sequences in Supplementary

(3) Epigenetic memory of colitis promotes tumour growthNatureMarch 25, 2026Surya Nagaraja, Lety Ojeda-Miron, Ruochi Zhang, Ena Oreskovic, Conrad Hock, Yan Hu, Daniel Zeve, Karina Sharma, Roni R. Hyman, Qiming Zhang, et al.empty barcode vector (2 μg) was digested and dephosphorylated with BamHI (NEB, R3136T), XbaI (NEB, R0145S) and FastAP (Thermo, EF0652) for 4–12 h at 37 °C. Following purification, 1 μg of digested plasmid and

(4) Characterization of HER2-Positive Murine Breast Cancer Models for Investigating HER2-Targeted Therapy and ImmunotherapyCancers (Basel)March 19, 2026Yun Lu, Benjamin P. Lee, Abbigael V. Eli, Shannon E. Lynch, Ar Rafi Md Faisal, Jonathan Moye, Anna G. Soraceplasmid (Addgene, #73582) and the PCR-amplified HER2WT or HER2YVMA insert were digested with XbaI (NEB, R0145L) and subsequently ligated using T4 DNA ligase (NEB, M0202S). The resulting plasmid maps are included

(5) Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecovirusesScientific ReportsMarch 18, 2026John A. Frank, Eric Xu Gan, William Brenham Hooper, Isabel Madeleine Ott, Akiko IwasakipCAGGS-BANAL52-S (MZ937000) were synthesized as gene blocks by Twist Bioscience and subcloned into XbaI (NEB R0145S) and NotI (NEB R3189S) double-digested pCAGGS plasmids by Gibson Assembly (NEB HiFi M5520). pTwist-SARS-CoV-2-BA1-S

(6) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie Pylesequences as described above were linearized with Xba1 (WT, SLII and DBI mutants, 3’-Terminus) (NEB, cat #R0145S) and EcoRI (SLIV and DBII mutants) (NEB, cat #R3101S). The linearized plasmid served as a template for

(7) DPEP2 suppresses hyperinflammation via metabolic reprogramming of macrophages in sepsisNature CommunicationsMarch 9, 2026Wenchen Luo, Wei Xu, Qimeng Yin, Jieqiong Song, Jie Wang, Yian Guan, Jian Huang, Jian Yu, Kefeng Zou, Danfeng Jin, et al.fragment and the pLVX-puro lentiviral expression vector78 (Addgene, 125839) were digested with XbaI (R0145S, New England Biolabs) and XhoI (R0146S, New England Biolabs), purified, and ligated using T4 DNA ligase.

(8) Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cellsScientific ReportsFebruary 20, 2026Nicole M. Jenkinson, Harsh Oza, Kevin J. Yarema, Kristine GlundeCampeau & Paul Kaufman, Addgene #17486) using restriction enzyme cloning with XbaI (New England Biolabs #R0145S) and SalI-HF (New England Biolabs #R3138S) using NEB rCutSmart Buffer (New England Biolabs #B6004S).

(9) Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytesSignal Transduction and Targeted TherapyFebruary 18, 2026Ziyin Zhang, Nanshu Xiang, Qian Liu, Yanfeng Li, Linhua Wang, Xinpu Yang, Ju Liu, Lichen Zhang, Liaoxun Lu, Yinming Liang, et al.were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by T4 ligation (Takara, 2011B). The plasmids expressing V5-tagged mouse RNF213

(10) HAMMER: Hairpin-based APOBEC3A-mediated mRNA editing reporterbioRxivFebruary 15, 2026Yanjun Chen, Christopher D. Mullally, Bojana Stefanovska, Reuben S. HarrisHairpin1 reporter linker region with HindIII (New England Biolabs, R3104L) and XbaI (New England Biolabs, R0145L) and inserting gBlocks containing candidate A3A hairpin substrates (gBlock sequences in Table S1). Stop1

(11) Integrase-On-Demand: bioprospecting integrases for targeted genomic insertion of genetic cargoNucleic Acids ResFebruary 9, 2026Hannah M McClain, Lillian C Lowrey, Laura B Quinto, Ellis L Torrance, Tomas R Gagliano, Farren J Isaacs, Joseph S Schoeniger, Kelly P Williams, Catherine M Mageeneyinto pUCP22 using restriction cloning (Eco53kI (NEB, Ipswich, MA, R0116S), XbaI (NEB, Ipswich, MA, R0145S), Monarch® Spin PCR & DNA Cleanup Kit (NEB, Ipswich, MA, T1130L), Ligase (NEB, Ipswich, MA, M0202L).

(12) Cancer cachexia in STK11/LKB1-mutated non-small cell lung cancer is dependent on tumor-secreted GDF15Nature CommunicationsJanuary 30, 2026Jinhai Yu, Tong Guo, Arun Gupta, Ernesto M. Llano, Thomas Salisbury, Naureen Wajahat, Dianne Zhao, Sean Slater, Qing Deng, Esra A. Akbay, et al.pLVX-EF1α-IRES-Hygro vector (kindly gifted by Shimeng Xu, UTSW Medical Center) by first treating with the XbaI (NEB, #R0145S) and BamH1-HF (NEB, #R3136S) restriction enzymes followed by In-Fusion Snap Assembly (Takara, #638947)

(13) sRNA196 promotes Pseudomonas aeruginosa virulence by regulating target genes PA4398, ZapE and PhoBMicrobial Cell FactoriesJanuary 27, 2026Fanghong Wang, Peng Ma, Peng Zhou, Yanmei Liu, Qiwei Li, Ling Zhang, Youqiang Li, Weiguo Yin, Juanjuan Hanfragment. Additionally, the suicide plasmid PGSM was double-digested with SacI (NEB, R3156) and XbaI(NEB, R0145L), and the purified product was recovered. Next, seamless cloning technology was used to insert the target

(14) A prophage-expressed type IV pilus component provides anti-phage defenseCell ReportsJanuary 27, 2026Kristina M. Sztanko, Taylor J. Ellison, Jill Greenlaw, Tatiana Lenskaia, P. Nathael Javorčík, Alexa D. Fitzpatrick, Karen L. Maxwell, Courtney K. Ellison, Alan R. Davidson,New England Biolabs,Cat# R3104S KpnI-HF,New England Biolabs,Cat# R3142S XbaI-HF,New England Biolabs,Cat# R0145S BsaI-HF v2,New England Biolabs,Cat# R3733S T4 DNA Ligase,New England Biolabs,Cat# M0202S T4 Polynucleotide Kinase

(15) Notch Signalling Plays a Role in Patterning the Ventral Mesoderm During Early Embryogenesis in Drosophila melanogasterInternational Journal of Molecular SciencesJanuary 27, 2026Marvel Megaly, Gregory Foran, Arsala Ali, Anel Turgambayeva, Tai Sengsouriya, Samantha Berube, Ryan Douglas Hallam, Ping Liang, Aleksandar NecakovUSA) by cutting the cassette out of pUASP-attb-Cry2-CTEV-mCherry using NotI and XbaI (NEB #R3189S and #R0145S, Whitby, ON, Canada) and ligating it to the linearized pAc5.1/V5-His A. The resultant plasmid was named

(16) Construction and Biological Characterization of ORF133-Deletion Mutant of Lumpy Skin Disease VirusVirusesJanuary 24, 2026Qunhua Ke, Miaomiao Li, Yao Peng, Xiangwei Wang, Yuefeng Sun, Xiangping Yin, Yanming WeiBuffer,P0013C HindIII-HF®,R3104S,Neb,Ipswich/MA/USA Neb,Ipswich/MA/USA,EcoRI-HF®,R3101T Neb,Ipswich/MA/USA,KpnI-HF®,R3142S Neb,Ipswich/MA/USA,XbaI,R0145S Neb,Ipswich/MA/USA,T4 DNA Ligase,M0202S WestemBright ECL,K-12045-D50,Advansta,Menlo Park/CA/USA Main

(17) Workflow for Gene Overexpression and Phenotypic Characterisation in Taraxacum kok-saghyzMethods and ProtocolsJanuary 24, 2026Loredana Lopez, Michele Antonio Savoia, Loretta Daddiego, Paolo Facella, Elio Fantini, Linda Bianco, Simone Maci, Francesco PanaraDarmstadt, Germany; Cat. no.: P1379).XbaI and SacI (New England Biolabs, Ipswich, MA, USA; Cat. no.: R0145L and R3156L). 2.2. Equipment Standard laboratory equipment: analytical balance; autoclave; electroporator

(18) Loss of ATM causes R-loop-associated transcriptional dysregulation and attenuates the related response to DNA damageJournal of Biological ChemistryJanuary 14, 2026Katherine R. Westover, Yingzi Hou, Feng Wang, Yilin Wang, Yangping Li, Rachel Seong, Jie Xu, Zhexing Wen, Bing Yao(BsrGI, EcoRI, HindIII, SspI, and XbaI, 30 units each) (NEB, #3575S, #R3101S, #R3104S, #R3132L, and #R0145S) at 37 °C overnight. The digested nucleic acids were then recovered by phenol–chloroform extraction. Dot blots

(19) Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosisNucleic Acids ResearchJanuary 8, 2026Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh, et al.kb 66% AT + PciI_RP by PCR (Supplementary Table S5). Amplified fragment was digested with XbaI (NEB R0145S) and PciI (NEB R0655S) restriction enzymes and ligated to the already digested 18-kb plasmid with the

(20) Global Evaluation of Congenital Heart Disease-Associated Non-Coding VariantsResearch SquareJanuary 7, 2026Edwin G. Rodríguez-Martínez, Shreya Peña-Martínez, Joshua G. Medina-Feliciano, Joshua Medina-Feliciano, Lois G. Parks, Marissa Granitto, Diego A. Pomales-Matos, Jean L. Pomales-Matos, Jean Messon- Barreiro-Rosario, Adriana Barreiro-Rosario, et al.Aldrich,276855 NEBNext® Ultra™ II Q5® Master Mix,NEB,M0544S Gibson Assembly Master Mix,NEB,E2611L XbaI,NEB,R0145S NcoI,NEB,R0193S RecBCD,NEB,M0345S AsiSI,NEB,R063L NEBNext Q5® High-Fidelity 2X Master Mix,NEB,M0492L Penicilin-Streptomycin,ThermoFisher,15140122 SDS,Sigma

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(1)

9/11/2015Good XbaI Restriction Enzyme J GThis is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.

XbaI

XbaI from New England Biolabs

Sequence

New England Biolabs

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XbaI from New England Biolabs

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