RT-PCR Grade Water from Thermo Fisher Scientific

Product Specs
ItemRT-PCR Grade Water
CompanyThermo Fisher Scientific
Price $79.25 Inquire
Catalog NumberAM9935
Quantity10 x 1.5 mL
StorageStore at -20°C
GradeMolecular Biology

Add to Compare List

Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Transplanted gene-modified placental cells boost FVIII activity in pediatric sheep without eliciting immunity, toxicity, or adverse eventsFrontiers in ImmunologyJanuary 7, 2026Brady Trevisan, Martin Rodriguez, Ritu Ramamurthy, Sunil George, Oluwaseun O. Babatunde, Jacqueline Dizon, Jordan Shields, Shannon Lankford, Denise Schwahn, Michael Gautreaux, et al.reaction volume of 9ul was achieved by adding nuclease-free PCR-grade water (ThermoFisher Scientific, AM9935). The reaction mixture was loaded into the Applied Biosystems™ QuantStudio™ MAP16 Digital PCR Plate

(2) Aptamer-CRISPR/Cas12a-Based Lateral Flow Technique for Visualized Rapid Detection of Endogenous Damage Factor Neu5Gc in Red MeatFoodsAugust 19, 2025Yuxi Guo, Honglin Ren, Han Wang, Xuepeng Duan, Shuaihao Qi, Xi Yang, Chunyi Shangguan, Haosong Li, Yansong Li, Pan Hu, et al.aptamer N2A9.55 sequence. DEPC-treated water: Purchased from Thermo Fisher, Waltham, MA, USA (Cat. #AM9935), used to avoid RNase contamination during crRNA handling. The LbCas12a (Lachnospiraceae bacterium Cas12a

(3) Single cell viral tagging of Faecalibacterium prausnitzii reveals rare bacteriophages omitted by other techniquesGut MicrobesAugust 4, 2025Danielle E. Campbell, Xiaofen Wu, Lindsey R. Hall, Dylan Lawrence, Jerome M. Molleston, Lawrence A. Schriefer, Lindsay Droit, James S. Weagley, Benjamin S. Olson, Joanna Rimmer, et al.Stratalinker 2400 prior to creating the master mix. First, 5.2 µL of DNase/RNase free water (Fisher Scientific AM9935), 2 µL of NEB 10X Buffer (NEB B0269S), 1 µL of Exo-resistant random hexamers (MCLAB ERRP-110), 0.5 µL

(4) Platelets in preeclampsia: an observational study of indices associated with aspirin nonresponsiveness, activation and transcriptional landscapeBMC MedJune 9, 2025Gashaw Garedew Woldeamanuel, Kenean Getaneh Tlaye, Xueqin Wang, Long Nguyen-Hoang, Qiongjie Zhou, Yinan Wang, Bo Wah Leung, Yao Wang, Liona C. Poon, Chi Chiu WangScientific). The concentration was adjusted to an equivalent of 10 ng/µl using RT‒PCR-grade water (AM9935, Invitrogen). The template RNA was combined with random hexamers and dNTP mix, briefly centrifuged,

(5) Effects of an Initial Anti-CD19 CAR T-cell Therapy on Subsequent Anti-CD22 CAR T-cell Manufacturing and Clinical Outcomes in Patients with Relapsed/Refractory LBCLCancer DiscoveryApril 2, 2025Yi-Jiun Su, Anne Marijn Kramer, Mark P. Hamilton, Neha Agarwal, Hrishikesh K. Srinagesh, John H. Baird, Bita Sahaf, Adam Kuo, Zachary J. Ehlinger, Moksha H. Desai, et al.TaqMan Fast Universal PCR Master Mix (2x), No AmpErase UNG or equivalent (Thermo Fisher Scientific) and 5 μL of TE buffer (catalog no. AM9935; Invitrogen). qPCR was performed using the QuantStudio® 6 Pro Real-Time PCR System (Thermo Fisher Scientific®),

(6) Three-dimensional linkage analysis with digital PCR for genome integrity and identity of recombinant adeno-associated virusSci RepJanuary 16, 2025Tam Duong, Michele Firmo, Chien-Ting Li, Bingnan Gu, Peng Wangtreatment can be subjected to ddPCR run, the sample is diluted in nuclease-free water (Life Technologies, AM9935) to the concentration range from 50 – 5000 copies/uL to get in the limit of quantification range of

(7) Intravenous and intracranial GD2-CAR T cells for H3K27M+ diffuse midline gliomasNatureNovember 13, 2024Michelle Monje, Jasia Mahdi, Robbie Majzner, Kristen W. Yeom, Liora M. Schultz, Rebecca M. Richards, Valentin Barsan, Kun-Wei Song, Jen Kamens, Christina Baggott, et al.(Thermo Fisher Scientific) and either 24.5 µl (albumin) or 22.5 µl (GD2.41BB.z) of TE buffer (Invitrogen, catalogue no. AM9935). The Thermo Fisher Scientific QuantStudio 6 Pro Real-time qPCR Instrument was used for qPCR, with 20

(8) An Automated Versatile Diagnostic Workflow for Infectious Disease Detection in Low-Resource SettingsMicromachines (Basel)May 28, 2024Miren Urrutia Iturritza, Phuthumani Mlotshwa, Jesper Gantelius, Tobias Alfvén, Edmund Loh, Jens Karlsson, Chris Hadjineophytou, Krzysztof Langer, Konstantinos Mitsakakis, Aman Russom, et al.manufacturer’s instructions with the final DNA being eluted in DNAse-free water (NFW) (Invitrogen; cat. no. AM9935, now ThermoFisher, Waltham, MA, USA), and cerebrospinal fluid (CSF) at a final concentration of 1000

(9) The S1 subunits of SARS-CoV-2 variants differentially trigger the IL-6 signaling pathway in human brain endothelial cells and downstream impact on microglia activationNeuroImmune Pharm TherMarch 1, 2024Stangis M, Adesse D, Sharma B, Castro E, Kumar K, Kumar N, Minevich M, Toborek M.(ThermoFisher). Then, 100 ng of RNA diluted in 1 μL of RT-PCR Grade Water (ThermoFisher Scientific, AM9935) was used for each reaction, with 7 μL of RT-PCR Grade Water, 10 µL qScript™ XLT One-Step RT-qPCR ToughMix®

(10) Sugar feeding by invasive mosquito species on ornamental and wild plantsSci RepDecember 13, 2023Irving Forde Upshur, Mikhyle Fehlman, Vansh Parikh, Clément Vinauger, Chloé Lahondèreinterest were amplified using PCR. Each sample was composed of 200 ng of DNA, PCR grade water (Fisher, AM9935), 10 μL of MyTaqHSmix (Bioline BIO-25045), 0.4 μM of forward and reverse primers targeting the chloroplast

(11) Epigenetic deregulation of MLF1 drives intrahepatic cholangiocarcinoma progression through EGFR/AKT and Wnt/β-catenin signalingHepatology communicationsAugust 1, 2023Tang Z, Yang Y, Chen W, Liang T.TaqMan® Universal Master Mix II (No UNG, Applied Biosystems, 4440040), RT-PCR Grade Water (Invitrogen, AM9935), and cDNA template was amplified using ABI QuantStudio-5 Real-Time PCR System (Applied Biosystems,

(12) CRISPR/Cas9-based Engineering of Immunoglobulin Loci in Hybridoma CellsBio-protocolFebruary 20, 2023Gall CLM, Fennemann FL, Van Der Schoot JMS, Scheeren FA, Verdoes M.M0491)dNTP (Thermo Fisher, catalog number: R0192)ddH2O (Thermo Fisher, Invitrogen, catalog number: AM9935)Agarose (Sigma-Aldrich, catalog number: A4718)Nancy-520 (Sigma-Aldrich, catalog number: 01494)NucleoBond Xtra Midi Plus EF

(13) Epigenetic dysregulation-mediated COL12A1 upregulation predicts worse outcome in intrahepatic cholangiocarcinoma patientsClinical epigeneticsJanuary 24, 2023Tang Z, Yang Y, Zhang Q, Liang T.Bulge-Loop miRNA qRT-PCR Starter Kit (C10211-1, RIBOBIO, Guangzhou, CN) reagents, RT-PCR Grade Water (AM9935, Applied Biosystems, Thermo Fisher Scientific, USA) and cDNA template was amplified using ABI QuantStudio-5

(14) Single-cell genomics for resolution of conserved bacterial genes and mobile genetic elements of the human intestinal microbiota using flow cytometryGut MicrobesDecember 31, 2022Dylan Lawrence, Danielle E. Campbell, Lawrence A. Schriefer, Rachel Rodgers, Forrest C. Walker, Marissa Turkin, Lindsay Droit, Miles Parkes, Scott A. Handley, Megan T. Baldridgeaddition of 1uL of 10 mM Tris, pH 4.0. Subsequently, 5.2uL of DNase/RNase free water (Fisher Scientific AM9935), 2uL of NEB 10X Buffer (NEB B0269S), 1uL of Exo-resistant random hexamers (MCLAB ERRP-110), 0.5uL of

(15) Evidence for Complex Interplay between Quorum Sensing and Antibiotic Resistance in Pseudomonas aeruginosaMicrobiology SpectrumDecember 21, 2022Rakesh Sikdar, Mikael H. Elias(Thermo Fisher Scientific) following the manufacturer’s instructions. RT-PCR-grade water (Ambion, no. AM9935) was used for setting up all reactions. Primers for PA14 folA, folP, and recA genes (Table S2) were

(16) Intravesicular Genomic DNA Enriched by Size Exclusion Chromatography Can Enhance Lung Cancer Oncogene Mutation Detection SensitivityInternational journal of molecular sciencesDecember 16, 2022Van Hoof R, Deville S, Hollanders K, Berckmans P, Wagner P, Hooyberghs J, Nelissen I.sample was diluted with RT-PCR grade water (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, #AM9935) to match the assay range and 1–20 µL was added to 199–180 µL of Qubit working solution. Before each

(17) Post-infusion CAR T(Reg) cells identify patients resistant to CD19-CAR therapyNature medicineSeptember 1, 2022Good Z, Spiegel JY, Sahaf B, Malipatlolla MB, Ehlinger ZJ, Kurra S, Desai MH, Reynolds WD, Wong Lin A, Vandris P, et al.TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG or equivalent (Thermo Fisher Scientific), and 5 µL TE buffer (Invitrogen #AM9935). The Bio-Rad CFX96 Touch Real-Time PCR Detection System was used for qPCR with 20 µL per reaction.

(18) Epidemiological and virological factors determining dengue transmission in Sri Lanka during the COVID-19 pandemicPLOS Global Public HealthAugust 3, 2022Dinuka Ariyaratne, Laksiri Gomes, Tibutius T. P. Jayadas, Heshan Kuruppu, Lahiru Kodituwakku, Chandima Jeewandara, Nimalka Pannila Hetti, Anoja Dheerasinghe, Sudath Samaraweera, Graham S. Ogg, et al.of each primer, 250 nM of each probe, 7 μl of RNA and PCR grade water (Applied Biosystems, USA, Cat: AM9935). After the primers and probes were validated, a multiplex method was optimized to quantify the four

(19) Smallpox vaccination induces a substantial increase in commensal skin bacteria that promote pathology and influence the host responsePLoS pathogensApril 1, 2022Shmeleva EV, Gomez de Agüero M, Wagner J, Enright AJ, Macpherson AJ, Ferguson BJ, Smith GL.corresponding to the day of tissue collection. DNA samples were diluted with nuclease free water (Ambion, Cat # AM9935, Austin, Texas) to normalise DNA concentrations to ~90 ng/μl. The NEXTflex 16S V4 Amplicon-Seq Kit 2.0

(20) GD2-CAR T cell therapy for H3K27M-mutated diffuse midline gliomasNatureMarch 31, 2022Robbie G. Majzner et al.TaqMan Fast Universal PCR Master Mix (2X), no AmpErase UNG (Thermo Fisher Scientific) and 24.5 µl (albumin) or 22.5 µl (GD2–4-1BB–CD3Ζ) of TE buffer (AM9935, Invitrogen). The Thermo Fisher Scientific QuantStudio 6 Pro Real-time qPCR Instrument was used for

Be the First to Write a Review!

RT-PCR Grade Water from Thermo Fisher Scientific

Description

Thermo Fisher Scientific

Citations (43)

Reviews

RT-PCR Grade Water from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map