pGEM-T Vector System II from Promega

Product Specs
ItempGEM-T Vector System II
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3610
HostBacteria
PromoterT7 and SP6 RNA polymerase promoters
MCSApa I, Aat II, Sph I, BstZ I, Nco I, Sac II, Spe I, Not I, BstZ I, Pst I, Sal I, Nde I, Sac I, BstX I, Nsi I
ResistanceAmpicillin
Origin of Replicationf1 Origin of Replication
Selectable Markerß-galactosidase
Quantity20 reactions
Vector NamepGEM®-T Vector

Specifications/Features

Kit Contents:
Includes:

• 1.2µg pGEM®-T Vector (50ng/µl)
• 12µl Control Insert DNA (4ng/µl)
• 100u T4 DNA Ligase
• 200µl 2X Rapid Ligation Buffer, T4 DNA Ligase
• 1.2ml JM109 Competent Cells, High Efficiency (6 × 200µl)
• 1 Protocol

Features:
• Rapid Ligation: The 2X Rapid Ligation Buffer provided allows reactions to be completed in 1 hour at room temperature.
• Blue/White Screening: T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the a-peptide coding region for ß-galactosidase. Insertional inactivation of the a-peptide allows recombinant clones to be directly identified by color screening on indicator plates.
• f1 Origin of Replication: Allows the preparation of single-stranded DNA.

Vector Size:
3,000 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Regulation of the MAPK/ERK Pathway by miRNA‐27b in Gastric Cancer: Diagnostic Implications and Therapeutic Potential of AloinAsia-Pacific Journal of Clinical OncologyDecember 1, 2025Yang Dehuo, Wang Ying, Cao LinF530S), the miRNA binding site‐containing 3′UTR segments was cloned into the pGEM‐T‐Easy (Promega, A3610) intermediate vector for sequence confirmation. GRB2, SOS1, KRAS, BRAF, MAP2K1, and MAPK1 3′UTR sequences

(2) Androgen receptor-regulated lncRNA PRCAT71 promotes AR signaling through the interaction with KHSRP in prostate cancerSci AdvApril 11, 2025Yongyong Yang, Ting-You Wang, Qianru Li, Jiawen Lu, Yanan Ren, Adam B. Weiner, Joshua Fry, Qi Liu, Chaehyun Yum, Rui Wang, et al.Germany) and were inserted into pGEM-T vector by performing T-vector cloning using a T-vector cloning kit (A3610, Promega, Madison, WI). Final pGEM-T plasmid DNA with insertion was sent to Northwestern Core Facility

(3) The inner junction protein CFAP20 functions in motile and non-motile cilia and is critical for visionNature communicationsNovember 3, 2022Chrystal PW, Lambacher NJ, Doucette LP, Bellingham J, Schiff ER, Noel NCL, Li C, Tsiropoulou S, Casey GA, Zhai Y, et al.extraction99 and PCR amplification surrounding the cut sites, followed by ligation into pGEM-T (Promega A3610) was performed followed by Sanger sequencing to identify mutations. Clutches with the ua5025 allele

(4) A Novel, Cleaved Probe-Based Reverse Transcription Loop-Mediated Isothermal Amplification Method for Specific and Sensitive Detection of Porcine DeltacoronavirusFrontiers in Veterinary ScienceJune 23, 2022Haiyan Shen, Songqi Wang, Jun Huang, Qijie Lin, Chunhong Zhang, Zhicheng Liu, Jianfeng Zhang, Ming Liaoinstructions. The amplified N gene of PDCoV was cloned into the pGEM-T Easy vector (Promega, Cat. A3600, A3610), which was used for the standard plasmid preparation. Plasmid DNA was extracted using the Plasmid Mini

(5) Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPRSTAR ProtocolsJune 17, 2022Yuhan Liu, Qian Chen, Chenglin Song, Zhen Xu, Shuting Yang, Xiajun LiTA cloning for cloning of the PCR product. These include the pGEM-T vector from Promega, USA (Cat. # A3610) and the TOPO TA cloning system from Invitrogen of Thermo Fisher Scientific, USA (Cat. # 451641). Expansion

(6) Genetic characterization of the spike gene of porcine epidemic diarrhea viruses (PEDVs) circulating in Vietnam from 2015 to 2016Veterinary Medicine and ScienceAugust 1, 2020Van T. Than, Se‐Eun Choe, Thi T. H. Vu, Tien D. Do, Thi L. Nguyen, Thi T. N. Bui, Thi N. Mai, Ra M. Cha, Daesub Song, Dong‐Jun An, et al.sequencing and sequence analysis The PCR products were cloned into the pGEM‐T Vector System II (Cat. No. A3610; Promega) according to the manufacturer's instructions. The cloned genes were sequenced with T7 and

(7) Spermine in semen of male sea lamprey acts as a sex pheromonePLOS BiologyJuly 9, 2019Anne M. Scott et al.based on the coding region of taar348. The amplified DNA fragments were cloned into a pGEM-T vector (A3610, Promega), and the sequences were verified. Each plasmid was linearized using restriction enzyme Nco

(8) PAX3 Confers Functional Heterogeneity in Skeletal Muscle Stem Cell Responses to Environmental StressCell stem cellJune 6, 2019Der Vartanian A, Quétin M, Michineau S, Auradé F, Hayashi S, Dubois C, Rocancourt D, Drayton-Libotte B, Szegedi A, Buckingham M, et al.5’-ACTCGAGCACTACAGTGGCGACTCAGATGC-3’, 5’-ATAACAGAGTTAGGTCTACAGGGCC-3’), then subcloned in pGEMTeasy (Promega #A3610) with a newly introduced XhoI site in-between. A nls-LacZ-SV40pA-FRT-Kanamycin-FRT cassette was then

(9) Inhibition of Inflammatory Signaling in Tet2 Mutant Preleukemic Cells Mitigates Stress-Induced Abnormalities and Clonal HematopoiesisCell stem cellDecember 6, 2018Cai Z, Kotzin JJ, Ramdas B, Chen S, Nelanuthala S, Palam LR, Pandey R, Mali RS, Liu Y, Kelley MR, et al.included in Supplemental Table-4). The amplified products were cloned into T-vector (Promega, Cat #A3610). Twenty-four proper clones were randomly selected for sequencing analysis to determine the methylation

(10) Bacterial microbiome of the chigger mite Leptotrombidium imphalum varies by life stage and infection with the scrub typhus pathogen Orientia tsutsugamushiPLOS ONEDecember 6, 2018Loganathan Ponnusamy, Alexandra C. Willcox, R. Michael Roe, Silas A. Davidson, Piyada Linsuwanon, Anthony L. Schuster, Allen L. Richards, Steven R. Meshnick, Charles S. AppersonIndianapolis, IN, USA) and purified DNA from amplicons was inserted into the plasmid vector pGEM-T (Cat. No A3610, pGEM–T Vector System II; Promega) as specified by the manufacturer. White colonies were picked and

(11) A novel strain of porcine deltacoronavirus in VietnamArchives of VirologyJanuary 1, 2018Van Phan Le et al.instructions, and products of the expected size were cloned into the pGEM-T Vector System II (Promega, Cat. No. A3610, USA). The cloned gene was then sequenced on an ABI Prism® 3730xi DNA Sequencer at the Macrogen Institute

(12) Caracterización molecular de péptidos antimicrobianos a partir de muestras de piel de Agalychnis spurrelli (Anura: Hylidae)Revista Ecuatoriana de Medicina y Ciencias BiológicasAugust 15, 2017Andrea P. Vargas, Óscar Pérez, David Ortega Paredes, Myrian RiveraA.spuF1-A. spuR2 y A.spuF1-AUAP (3’RACE) se ligaron al vector pGEM®-T Vector System (PROMEGA Cat. No. A3610), por poseer una región de resistencia a la ampicilina y alfa complementación. El protocolo fue realizado

(13) INSERSI GEN pncA KE DALAM PLASMID pGEM-TJurnal Kimia RisetDecember 21, 2016Eli Hendrik Sanjaya(2009): pGEM®-T and pGEM®T Easy Vector Systems: Instructions for Use of Products A1360, A1380, A3600 AND A3610.Technical Manual No. 042.Printed in USA.Revised 6/09. Sanjaya, E.H. (2012): MUTASI T539C GEN pncA (Val180Ala)

(14) LPS-induced expression of CD14 in the TRIF pathway is epigenetically regulated by sulforaphane in porcine pulmonary alveolar macrophagesInnate ImmunityNovember 1, 2016Qin Yang et al.PCR. The puriﬁed bisulﬁte PCR products were cloned using pGEMÕ -T Easy Vector Systems (Promega GmbH, A3610). Puriﬁed bisulﬁte PCR products (3 ml) were used for the ligation, and 100 ml of each transformation

(15) Generation and characterization of monoclonal antibody against Advanced Glycation End Products in chronic kidney diseaseBiochemistry and Biophysics ReportsMarch 21, 2016Alessandra Becker Finco, Ricardo Andrez Machado-de-Ávila, Rayana Maciel, Juliana De Moura, Philippe Billiald, Andrea Emilia Marques Stinghen, Larissa M. Alvarengachain variable VL amplification. PCR products were inserted into the cloning vector pGEM-T (Promega A3610) before being sequenced and analyzed according to the protocol previously reported [17]. To compare

(16) Cloning, Sequencing, and Expression of Selenoprotein Transcripts in the Turkey (Meleagris gallopavo)PLOS ONEJune 12, 2015Roger A. Sunde, Gavin R. Sunde, Colin M. Sunde, Milton L. Sunde, Jacqueline K. EvensoncDNA (3 μl of the 24–25 μl reaction) was ligated into pGEM-T vector (pGEM-T Vector System II, Promega A3610) following manufacturer’s protocol. Following overnight incubation at 4°C, 2 μl (of the 10 μl reaction)

(17) Prevalence of Rickettsiales in ticks removed from the skin of outdoor workers in North CarolinaParasites & VectorsDecember 23, 2014Sangmi Lee, Madhavi L Kakumanu, Loganathan Ponnusamy, Meagan Vaughn, Sheana Funkhouser, Haley Thornton, Steven R Meshnick, Charles S Appersonsets of samples, purified DNA for each amplicon was inserted into the plasmid vector pGEM-T (Cat. No A3610, pGEM–T Vector System II; Promega) as specified by the manufacturer. White colonies were picked and

(18) Phylogenetic analysis of bovine astrovirus in Korean cattleVirus GenesApril 1, 2014Jae-Ku Oem, Dong-Jun AnProducts with the expected size were cloned using the pGEM-T Vector System IITM (Promega, Cat. No. A3610, USA). The cloned gene was sequenced with T7 and SP6 sequencing primers using an ABI PrismÒ 3730XI DNA Sequencer

(19) Phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in South KoreaVirus GenesFebruary 1, 2013Meong-Hun Lee, Hye-Young Jeoung, Hye-Ran Park, Ji-Ae Lim, Jae-Young Song, Dong-Jun An°C. Products of the expected size were cloned with the pGEM-T Vector System II™ (Promega, Cat. No. A3610, USA). The cloned gene was sequenced with T7 and SP6 sequencing primers on an ABI Prism® 3730xi DNA

(20) Identification and Characterization of a Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) Thermotolerance-Related Gene: Bx-HSP90International Journal of Molecular SciencesJuly 16, 2012Feng Wang, Zhiying Wang, Danlei Li, Qiaoli Chenthe pGEM-T vector and transformed into Escherichia coli JM109 competent cells (Promega, USA, cat. No. A3610). All of the recombinant clones were included to establish the subtracted cDNA library. Random sequencing

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pGEM-T Vector System II from Promega

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pGEM-T Vector System II from Promega

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