pRL-null Vector from Promega

Product Specs
ItempRL-null Vector
CompanyPromega
Price $77.00Supplier Page View Company Product Page
Catalog NumberE2271
HostMammalian
MCSBgl II, Xho I, Sac I, Nde I, EcoICR I, Hind III, Nsi I, Sph I, Spe I, Nar I, Sal I, Mlu I, EcoR I, Xma I, Sma I, Pst I
ResistanceAmpR
PromoterT7 promoter
ReporterRenilla luciferase
Vector NamepRL-null
Molecular Weight~66kDa
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Selectable MarkerAmpicillin
Quantity20microg

Specifications/Features

Vector Size:
AF025844

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Pharmacological HIF-PH Inhibition Suppresses Myoblast Differentiation Through Continued HIF-1α Stabilization †Int J Mol SciJune 5, 2025Yuya Miki, Akinobu Ochi, Hideki Uedono, Yoshinori Kakutani, Mitsuru Ichii, Yuki Nagata, Katsuhito Mori, Yasuo Imanishi, Tetsuo Shoji, Tomoaki Morioka, et al.pGL4.42[luc2P/HRE/Hygro] firefly luciferase reporter plasmid (E400A, Promega) and 20 ng of pRL-null Vector (E2271, Promega). After 24 h of FG-4592 treatment, we measured luciferase activity with a Dual-Luciferase Reporter Assay System

(2) Fbxo16 mediates degradation of NF-κB p65 subunit and inhibits inflammatory response in dendritic cellsFront ImmunolJune 3, 2025Akiko Sugimoto-Ishige, Aya Jodo, Takashi TanakaMyD88 (#13093) and human IKKβ (#23298) were purchased from Addgene. The pRL-Null renilla construct (#E2271) was purchased from Promega. The ELAM-1 luciferase reporter construct was provided by D. Golenbock (23). 2.2

(3) Systemic genome-epigenome analysis captures a lineage-specific super-enhancer for MYB in gastrointestinal adenocarcinomaMolecular Systems BiologyApril 15, 2025Fuyuan Li, Shangzi Wang, Lian Chen, Ning Jiang, Xingdong Chen, Jin Lireporter constructs were co-transfected with a control luciferase construct, named Renilla (Promega, #E2271), into cells using PEI (Polysciences, #24765) or lipofectamine 3000 (Invitrogen, #L3000015). The luciferase

(4) ETV2/ER71 regulates hematovascular lineage generation and vascularization through an H3K9 demethylase, KDM4AiScienceJanuary 17, 2025Min Seong Kim, Raham Lee, Dong Hun Lee, Heesang Song, Taekyung Ha, Joo Kyung Kim, Bum-Yong Kang, Karl Agger, Kristian Helin, Donghyun Shin, et al.promoter/enhancer,Kappelet al.38,N/A pMD2.G,Addgene,Cat# 12259 pMDLg/pRRE,Addgene,Cat# 12251 pRL-null,Promega,Cat# E2271 pRSV-Rev,Addgene,Cat# 12253 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Fiji,ImageJ,https://imagej.net/software/fiji/ FlowJo,FlowJo,https://www.flowjo.com/ GraphPad

(5) Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancerNat CommunJanuary 6, 2025Lukas Klein, Mengyu Tu, Niklas Krebs, Laura Urbach, Daniela Grimm, Muhammad Umair Latif, Frederike Penz, Anna Blandau, Xueyan Wu, Rebecca Diya Samuel, et al.(Paul Dobner, University of Massachusetts), 15 ng of pRL-null Renilla luciferase control reporter (#E2271, Promega; for background normalization), and compensated with empty vector control (pCMV2c, David Russell,

(6) Functional analysis of protein interactions using coupled bi-fluorescence complementation/GFP nanobody techniquesNucleic Acids ResearchAugust 12, 2024Tetsuaki Miyake, John C McDermott10ng of the mammalian expression vector constructs and 3 ng of no-promoter renilla luciferase vector (E2271 Promega) were mixed in Opti-MEM, and the mixture was combined with polyethylenimine (PEI, Linear (MW

(7) Functional Analysis of KAP1/TRIM28 Requirements for HIV-1 Transcription ActivationVirusesJanuary 13, 2024Keyera Randolph, Usman Hyder, Ashwini Challa, Erick Perez, Iván D’Orsotransfected with pcDNA3.1-HIV-1–LTR-FFL LUC reporter vector (50 ng/well), pRL-Null (Promega, catalog E2271) (5 ng/well), pcDNA4/TO GFP:SF (50 ng/well), pcDNA4/TO KAP1:F (50 ng/well) and/or pcDNA4/TO KAP1 deletion

(8) Quantitative trait loci on chromosomes 9 and 19 modulate AII amacrine cell number in the mouse retinaFrontiers in neuroscienceJanuary 1, 2023Kulesh B, Bozadjian R, Parisi RJ, Leong SA, Kautzman AG, Reese BE, Keeley PW.RRID:Addgene_12456), and (4) the transfection control Renilla luciferase-expression plasmid (pRL-null; #E2271; Promega). Except for the latter, which was transfected at 20 ng per well, all plasmids were transfected

(9) CIITA promoter polymorphism impairs monocytes HLA-DR expression in patients with septic shockiScienceNovember 18, 2022Miatello J, Lukaszewicz AC, Carter MJ, Faivre V, Hua S, Martinet KZ, Bourgeois C, Quintana-Murci L, Payen D, Boniotto M, et al.Reporter Vectors TK,Promega,E2241 pRL Renilla Luciferase Control Reporter Vectors no promoter,Promega,E2271 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Arlequin ver 3.11,"Excoffier

(10) RBFOX2-regulated TEAD1 alternative splicing plays a pivotal role in Hippo-YAP signalingNucleic Acids ResearchAugust 26, 2022Sunkyung Choi, Hyo Seong Lee, Namjoon Cho, Inyoung Kim, Seongmin Cheon, Chungoo Park, Eun-Mi Kim, Wantae Kim, Kee K Kimco-transfected with the 8 × GTIIC-Luc reporter plasmid (#34615; Addgene), pRL-null control plasmid (#E2271; Promega), and each indicated plasmid or siRNA. At 48 h post-transfection, luciferase activity was measured

(11) Identification of ELK1 interacting peptide segments in the androgen receptorThe Biochemical journalJuly 29, 2022Soave C, Ducker C, Kim S, Strahl T, Rosati R, Huang Y, Shaw PE, Ratnam M.in the CheckMate Mammalian Two-hybrid System (E2440). The pRL plasmid encoding Renilla luciferase (E2271) was purchased from Promega. The pCDH-CMV-MCS-EF1-puro cDNA Cloning and Expression Lentivector plasmid

(12) Genetic Aberrations and Interaction of NEK2 and TP53 Accelerate Aggressiveness of Multiple MyelomaAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2022Feng X, Guo J, An G, Wu Y, Liu Z, Meng B, He N, Zhao X, Chen S, Zhu Y, et al.(positive control, Promega), pGL3‐NEK2‐P6, pGL3‐NEK2‐P6M, and the internal control Renilla (pRL‐null, #E2271, Promega) vectors were co‐transfected with wild type pcDNA3.1‐TP53‐FLAG and the TP53 expression vector

(13) bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathwayRedox biologyMay 1, 2021Chen G, An N, Ye W, Huang S, Chen Y, Hu Z, Shen E, Zhu J, Gong W, Tong G, et al.constructs driven by NFκB binding sequences and Renilla luciferase pRL-TK (Int-) plasmid were used (Promega, E2271). Lysates were analyzed with the Dual-Luciferase Reporter Assay System (Promega, E1910). Firefly luciferase

(14) PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κBFrontiers in immunologyJanuary 1, 2020Jodo A, Shibazaki A, Onuma A, Kaisho T, Tanaka T.luciferase reporter construct was kindly provided by D. Golenbock (14). The pRL-Null renilla construct (#E2271) was from Promega. The pGL4.32-Null renilla construct was generated by deleting promoter region from

(15) Destabilizing NEK2 overcomes resistance to proteasome inhibition in multiple myelomaThe Journal of clinical investigationJuly 2, 2018Franqui-Machin R, Hao M, Bai H, Gu Z, Zhan X, Habelhah H, Jethava Y, Qiu L, Frech I, Tricot G, et al.(negative control), luciferase (under p65-dependant promoter, pGL3-2×NF-κB-Luc), and Renilla (pRL-null, E2271; Promega) (internal control) vectors were transfected into H1299 cells using Lipofectamine 2000. After

(16) Novel galeterone analogs act independently of AR and AR-V7 for the activation of the unfolded protein response and induction of apoptosis in the CWR22Rv1 prostate cancer cell modelOncotargetOctober 24, 2017David J. McCarty, Weiliang Huang, Maureen A. Kane, Puranik Purushottamachar, Lalji K. Gediya, Vincent C.O. Njar(ARR2-Luc; kindly provided by Dr. Yun Qiu) while the Renilla luciferase reporter is pRL-null (Promega, #E2271). A single bacterial colony containing each reporter was selected and grown in 40 mL LB broth overnight

(17) Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell LineOxidative Medicine and Cellular LongevityJune 2, 2016Hirotaka Yamamoto, Katsutaro Morino, Lemecha Mengistu, Taishi Ishibashi, Kohei Kiriyama, Takao Ikami, Hiroshi Maegawaluciferase reporter plasmid (pGL4.26 luc2/minP/Hygro, E8441) and internal control plasmid (pRL-CMV, E2271) were from Promega (Madison, WI, USA). The DNA sequence for the ARE was obtained from the National Center

(18) The RNA-binding protein Sam68 regulates expression and transcription function of the androgen receptor splice variant AR-V7Scientific reportsAugust 27, 2015Stockley J, Markert E, Zhou Y, Robson CN, Elliott DJ, Lindberg J, Leung HY, Rajan P.minigenes8; pLKO-si-Sam6829; and control pLKO-puro Non-Target shRNA Control (SHC016, Sigma) and pRL-null (E2271, Promega). pM-Sam68 was generated by cloning of the Sam68 coding sequence from pGBTK7-Sam6833 into the

(19) Involvement of microRNAs in the regulation of muscle wasting during catabolic conditionsThe Journal of biological chemistryAugust 8, 2014Soares RJ, Cagnin S, Chemello F, Silvestrin M, Musaro A, De Pitta C, Lanfranchi G, Sandri M.acquired from Signosis BioSignal. Renilla Null and Renilla TK were acquired from Promega (pRL-null number E2271, pRL-TK number E2241). The mature sequences of miRNA-206 and miRNA-21 were cloned into the BLOCK-iT Pol II miR RNAi Expression Vector Kit with EmGFP

(20) Preparation of a highly active cell-free translation system from immature Xenopus laevis oocytesMethodsMay 1, 2010Catherine A. Pratt, Kimberly L. MowryRenilla luciferase mRNA. The Renilla luciferase gene is available in the pRL-null plasmid from Promega (# E2271). This plasmid contains a T7 viral transcriptional promoter, and thus control reporter mRNA can be generated

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pRL-null Vector

pRL-null Vector from Promega

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Promega

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pRL-null Vector from Promega

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