pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibilityiScienceMay 15, 2026Xiaohan Liu, Yanxia Fu, Liuxuan Huang, Donghan Li, Paul Yao, Liqin Zengluciferase reporter vector,Promega,Cat# E1751 Renilla luciferase control vector (pRL-TK),Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, ImageJ software,National

(2) MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesisSci AdvApril 24, 2026Sihan Li, Zehua Wang, Phuong Nguyen, Yue Wang, Yueshan Zhao, Yifei Wang, Yuang Chen, Haozhe Huang, Xiaofei Wang, Yu Zhang, et al.transfected into MCF-7 cells using Lipofectamine 2000, and plasmid pRL-TK expressing Renilla (Promega, E2241) was used as an internal control reporter. After transfection for 24 hours, cells were subjected to

(3) Integrated drivers of basal and acute immunity in diverse human populationsCell Reports MedicineApril 21, 2026Aisha Hegab Souquette, E. Kaitlynn Allen, Christine M. Oshansky, Evadnie Rampersaud, Emily V. Walker, Li Tang, Sook-San Wong, Trushar Jeevan, Lei Shi, Stanley Pounds, et al.CO2. The following day, 1μg of pGL4.10 test plasmid and 0.01μg of pRL-TK control plasmid (Promega Cat. E2241) were added per well, according to the TransIT LT protocol. All transfections were conducted in triplicate.

(4) Secretory VSMC-derived THBS1 promotes macrophage M1 polarization and inflammation in intracranial aneurysms via the CD47/NF-κB axisFront ImmunolApril 10, 2026Jianhuang Huang, Qixiu Wang, Yangyang Lin, Jianning Chen, Caihou Linluciferase reporter plasmid (Clontech, 631744) and 50 ng/well pRL-TK Renilla luciferase plasmid (Promega, E2241) as an internal control. After 48 h of co-culture, cells were lysed and luciferase activities were measured

(5) ZXDB Drives Macrophage Inflammatory Programming in Sepsis-Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA TranslationFASEB JMarch 31, 2026Haiyang Wu, Wei Gang, Li Wang, Jing Sun, Shuangxi Li, Zhiyong Guoco‐transfected with the ACACA 5′UTR reporter construct, a control Renilla luciferase plasmid (pRL‐TK, E2241; Promega), and corresponding expression plasmids (ZXDB OE, EIF4A3 KD). Firefly luciferase activity was

(6) Oligomeric lactic acid nanoplastics induce intrauterine growth restriction in mice by disrupting GATA2-mediated placental vascular developmentPLoS BiolMarch 26, 2026Jia Lv, Mengjing Wang, Changzhi Shi, Yanwei Wang, Yihao Zhang, Chang Gao, Tian-Qi Bi, Jing Yang, Youdong Xu, Qunan Wang, et al.Cellmax, USA) and co-transfected with VEGFR2-WT, GATA2-pcDNA3.1, and the Renilla control plasmid pRL-TK (E2241, Promega, USA) using Lipofectamine 2000 (11668019, Invitrogen, USA). After 48 h, Firefly and Renilla

(7) ZBTB7A promotes malignant phenotypes in ovarian cancer through transcriptional activation of CRLF1Oncology ReportsMarch 24, 2026Xiaobai Hao, Yu Chen400 ng pGL3-CRLF1-Luc reporter construct, 100 ng pRL-TK Renilla luciferase control vector (cat. no. E2241; Promega Corporation) and 500 ng either ZBTB7A-pcDNA3.1 or empty pcDNA3.1 vector (as a control) using

(8) Oncogenic GPRIN1 sustains proliferation and mitochondrial homeostasis via dual‑layer CDK1-PI3K/Akt signalling in gallbladder cancerCell Death & DiseaseMarch 21, 2026Chang Xu, Zijun Gong, Xiaojian Ni, Lingxi Nan, Wentao Sun, Houbao Liu, Xuanming Luo, Min Lico-transfected with reporter plasmids, a Renilla luciferase internal control plasmid (pRL-TK, Promega, Cat# E2241), and GPRIN1/E2F1 expression vectors or empty vector. After 48 h, luciferase activity was measured using

(9) Tamoxifen differentially modulates endometrial hyperplasia via wild-type and mutant p53 regulation of the ALKBH5-REG1A axisFrontiers in OncologyMarch 19, 2026Rencheng Wang, Jianhua Ji, Lei Liuco-transfected with firefly luciferase constructs and Renilla luciferase plasmid (pRL-TK, Promega, E2241) as an internal control using Lipofectamine™ 3000 (Invitrogen). Twenty-four hours post-transfection,

(10) 4-(5-Chloro-3-(3,4,5-trimethoxybenzoyl)-1H-indol-1-yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β-Catenin Signaling Pathway, and P-GlycoproteinChemMedChemFebruary 25, 2026Michela Puxeddu, Rosa Bordone, Claudia Colla, Gabriele Rotili, Antonio Coluccia, Pietro Sciò, Petra Cuřínová, Alessio Nocentini, Claudiu T. Supuran, Serena Filiberti, et al.FOP control plasmid (M51 Super 8x FOPFlash Addgene #12457) in combination with TK Renilla (Promega #E2241) using DreamFect Gold (OZ Biosciences #DG80500) according to the manufacturer's instructions. After

(11) Semaphorin 6D drives anti-tumor type I interferon responses to reprogram the tumor microenvironment in colorectal cancerCell Death & DiseaseFebruary 24, 2026Wei Shi, Fan Zhang, Wei-Qing Sun, Li-Qi Liang, Shamalagowri Krishnanluciferase reporter construct (pGL3-ISRE) and the Renilla luciferase normalization vector (pRL-TK; Promega #E2241) at a 10:1 ratio using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Firefly and Renilla luciferase

(12) Lung cancer-enriched p53 mutants occupy canonical p53 target genes without activating transcription, revealing a distinct loss-of-function behaviorbioRxivFebruary 20, 2026Mason A. Tracewell, Hailey N. Shankle, Samantha M. Barnada, Khushali S. Vyas, Kevin M. Kim, Theodhora Qyshkollari, Jonathan E. Karlin, Julie A. Barta, Steven B. McMahonvectors (WT, V157F, R158L, or EV), and 200ng of pRL-TK Renilla luciferase control reporter vector (E2241, Promega) using Lipofectamine 3000 (L3000015, Invitrogen) according to manufacturer’s instructions.

(13) Tumor-immune-neural circuit disrupts energy homeostasis in cancer cachexiaCancer CellFebruary 12, 2026Xiuhui Shi, Alex X. Arreola, Zhijun Zhou, Jingxuan Yang, Mingyang Liu, Yang Cai, Yu Ren, Hao Yuan, Qun Chen, Xinjie Chen, et al.ZFP64-ORF expression vector (#EX-Y4764-Lv207, Genecopoeia), pGL4.10-ZIP4 vector, and pRL-TK Vector (#E2241, Promega) using Lipofectamine 3000 Reagent (#L3000015; Invitrogen). At 48 h after transfection, the

(14) KRAB Zinc‐Finger Protein ZNF205 Promotes Hepatocellular Carcinoma via p53 Pathway RepressionCancer ScienceFebruary 10, 2026Xiaofen Huang, Yingchuan Yang, Yuan Ma, Wei Hao, Jingzhuo Jin, Yue Ding, Yiming Zhang, Xiuyuan Zhang, Xinli Li, Qin Song, et al.with other expression plasmids and the pRL‐TK Renilla luciferase reporter plasmid for normalization (E2241, Promega, USA), were co‐transfected into p53+/+ HCT116 cells. After 48 h, cells were lysed in Passive

(15) TFE3 fusion proteins drive TFE3 rearranged renal cell carcinoma progression via PGC-1α-mediated fatty acid oxidationFrontiers in ImmunologyFebruary 4, 2026Fan Feng, Yanhao Xu, Zhenggen Deng, Xiang Dong, Guijuan Chen, Wenliang Ma, Dongmei Li, Weidong Ganwhich contains the firefly luciferase gene, using Lipofectamine 2000. The PRL-TK plasmid (Promega, E2241) was co-transfected at a 100:1 ratio as an internal control. After 48 hours of transfection, cells were

(16) Transcriptomic landscape of transposable elements reveals LTR7 - PLAAT4 as a potential oncogene and therapeutic target in pancreatic adenocarcinomaGenome ResearchFebruary 3, 2026Meilong Shi, Chuanqi Teng, Shan Zhang, Xiaobo He, Lingyun Xu, Fengxian Han, Rongqi Wen, Ganjun Yu, Jingwen Liu, Yang Feng, et al.deliver 400 ng of the promoter-luciferase vector and 200 ng of the pRL-TK Renilla vector (Addgene, #E2241) into 3 × 104 AsPC-1 cells and 3 × 104 293T cells. Luciferase levels were measured after 24 h of incubation

(17) The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathwayJournal of VirologyFebruary 3, 2026Jianyu Zeng, Xin Zhang, Jingting Zhao, Qiang Li, Zhiyong Xiang, Fengyang Shi, Hua Wang, Wenlian Weng, Qiongqiong Zhou, Peng Gao, et al.ng of ASFV structural protein plasmid, 400 ng pAP-1-Luc reporter vector, and 8 ng pRL-TK (Promega, E2241) per well using Lipofectamine 2000 for 36 h. Inhibitors or DMSO (control) were added 12 h post-transfection.

(18) TGFβ1 Activates Lnc‐APUE to Promote Tumor Metastasis via the Alu Element‐Driven STAU1‐Mediated Decay of CDH1 mRNAAdvanced ScienceFebruary 3, 2026Song‐Yang Li, Jia‐Hui Huang, Jin‐E Yang, Yi‐Hang Li, Juan‐Zhen Hong, Ting‐Ting Wang, Ying‐Lei Chi, Meng‐Zhi Wu, Wei Wang, Ying Zhu, et al.P(‐505/+70), P(‐212/+70), P(delSBE1/2), P(delSBE3) and P(mutSBE1/2). For normalization, the pRL‐TK vector (E2241, Promega) expressing Renilla luciferase was used as a control. 4.4 Cell Lines Human embryonic kidney

(19) Picornavirus VP2 protein suppresses innate immunity through selective autophagic degradation of IKBKE/IKKεAutophagyFebruary 1, 2026Kangli Li, Xiangle Zhang, Dandan Dong, Boning Zhu, Shuo Wang, Xiaodan Wen, Weijun Cao, Yi Ru, Hong Tian, Guoliang Zhu, et al.transfected with a combination of 50 ng of reporter plasmid (IFNB, ISRE), 5 ng of pRL-TK (Promega, E2241) serving as an internal control, and 50 ng of the specified plasmid. At 24 h post transfection (hpt),

(20) Single-cell analysis identifies BASP1 as a driver of drug resistance and cell plasticity in oral squamous cell carcinomaJournal of Biological ChemistryFebruary 1, 2026Abinash Behera, Sudeshna Datta, Sibasish Mohanty, Pallavi Mohapatra, Shamima Azma Ansari, Sreeparna Podder, Rachna Rath, Dillip Kumar Muduly, Rajeeb K. Swain, Sunil K. Raghav, et al.was a gift from Randall Moon (Addgene, plasmid # 12456) (47) and the pRL-TK Renilla (Promega, Cat No# E2241) luciferase control plasmid at a 100:1 ratio using ViaFect transfection reagent. The transfection was

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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pRL-TK Vector from Promega

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