pFN21A HaloTag CMV Flexi Vector from Promega

Product Specs
ItempFN21A HaloTag CMV Flexi Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG2821
Domain/Region/TerminusN term.
HostMammalian
Origin of ReplicationColE1-derived plasmid origin of replication
Quantity20microg
PromoterCMV promoter
Vector NamepFN21A
ResistanceAmpicillin
Selectable MarkerBarnase gene

Specifications/Features

Vector Size:
5064 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Proximity labeling reveals non-catalytic interactions between DPP9 and ubiquitin signaling complexesCell Mol Life SciFebruary 4, 2026Valentina Elena Wirtgen, Layla Saied, Samuel Zolg, Marta Campos Alonso, Bettina Mayer, Laura Donzelli, Ulrich Maurer, H. T. Marc Timmers, Klaus-Peter Knobeloch, Oded Kleifeld, et al.study, pFC32K_CYLD-NanoLuc,This study, pFC32K_SPATA2-NanoLuc,This study, pFN21A HaloTag® CMV Flexi® Vector,Promega,G2821 pFN21A_HaloTag-ABRO1,This study, pFN21A_HaloTag-BRCC36,This study, pFN21A_HaloTag-CYLD,This study, pFN21A_HaloTag-DPP9-S,This

(2) Single-molecule imaging quantifies oncogenic KRAS dynamics for enhanced accuracy of therapeutic efficacy assessmentiScienceSeptember 19, 2025Ryoma Yokoi, Toshiki Mori, Koichiro M. Hirosawa, Ryojiro Kondo, Tomohiko Taguchi, Nobuhisa Matsuhashi, Kenichi G.N. Suzukiby Azenta Life Sciences according to the sequence in Ando et al.72,N/A pFN21A-HaloTag7,Promega,Cat# G2821 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, 4-D Nucleofector,Lonza,Cat#

(3) Blebs promote cell survival by assembling oncogenic signalling hubsNatureMarch 16, 2023Andrew D. Weems, Erik S. Welf, Meghan K. Driscoll, Felix Y. Zhou, Hanieh Mazloom-Farsibaf, Bo-Jui Chang, Vasanth S. Murali, Gabriel M. Gihana, Byron G. Weiss, Joseph Chi, et al.the same 22 amino acid linker. SEPT6-HALO was made by cloning the HALO tag from pFN21K (Promega cat# G2821) onto the N-terminus of SEPT6 from SEPT6-GFP-pLVX-IRES-puro, replacing eGFP but maintaining the same

(4) Purification and enzymatic assay of class I histone deacetylase enzymesMethods in enzymologyJanuary 1, 2019Adams MK, Banks CAS, Miah S, Killer M, Washburn MP.,Thermo Scientific,Thermo Scientific ,15338100,, 13.,pFN21A HaloTag® CMV Flexi® Vector,Promega,Promega ,G2821,, 14.,pFC14A HaloTag® CMV Flexi® Vector,Promega,Promega ,G9651,, 15.,Mammalian Lysis Buffer,Promega,Promega ,G938A,, 16.,Protease

(5) A novel somatic mutation of SIN3A detected in breast cancer by whole-exome sequencing enhances cell proliferation through ERα expressionScientific reportsOctober 30, 2018Watanabe K, Yamamoto S, Sakaguti S, Isayama K, Oka M, Nagano H, Mizukami Y.antibody (sc-5299) and ESR1 siRNA (human, sc-29305) were purchased from Santa Cruz (CA). Plasmids pFN21A (G2821) and pMACS4.1 were purchased from Promega (Madison, WI) and (Miltenyi Biotec., Bergisch Gladbach, Germany),

(6) Real-Time Ligand Binding of Fluorescent VEGF-A Isoforms that Discriminate between VEGFR2 and NRP1 in Living CellsCell Chemical BiologyOctober 18, 2018Chloe J. Peach, Laura E. Kilpatrick, Rachel Friedman-Ohana, Kris Zimmerman, Matthew B. Robers, Keith V. Wood, Jeanette Woolard, Stephen J. Hillconstructs, wildtype VEGFR2 or NRP1 cDNA was cloned into a pFN21A CMV/neo flexi vector (Promega Corporation; G2821) encoding a fusion of the secretory signal peptide sequence of IL-6 onto the N terminus of HaloTag.

(7) Real-time analysis of the binding of fluorescent VEGF(165)a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexesBiochemical pharmacologyJuly 15, 2017Kilpatrick LE, Friedman-Ohana R, Alcobia DC, Riching K, Peach CJ, Wheal AJ, Briddon SJ, Robers MB, Zimmerman K, Machleidt T, et al.generated by Gene Dynamics LLC (Oregon, USA) and sub cloned into a modified pFN21 HaloTag CMV Flexi Vector (G2821; Promega Corporation, USA) containing an IL6 secretion signal. The linker sequence separating the two

(8) Stable complex formation of CENP-B with the CENP-A nucleosomeNucleic Acids ResearchMay 26, 2015Risa Fujita et al.50kb) (total 400 ng) and the Halo-CENP-A expressing plasmid (CENP-A inserted into pFN21A: Promega, G2821) (25 ng) were co-transfected into 70% confluent HT1080 tetR-EYFP-HJURP expressing cells in 6 well plates

(9) Genetically Encoded Multispectral Labeling of Proteins with Polyfluorophores on a DNA BackboneJournal of the American Chemical SocietyApril 24, 2013Vijay Singh, Shenliang Wang, Ke Min Chan, Spencer A Clark, Eric T Koolα-tubulin gene between the NcoI and BamHI sites of commercially available HaloTag plasmid pFN21A (Promega, G2821), a mammalian expression vector. The plasmid encoding cell surface-HaloTag fusion protein was obtained

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