pGloSensor-22F cAMP Plasmid from Promega

Product Specs
ItempGloSensor-22F cAMP Plasmid
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2301
HostMammalian
Origin of ReplicationColE1-derived plasmid origin of replication
ResistanceAmpicillin
Quantity20microg
Selectable MarkerAmpicillin
Vector NamepGloSensor-22F
PromoterCMV
Molecular Weight~86kDa
TypeEnzyme

Specifications/Features

Vector Size:
6990 bp

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Sphingosine‑1‑phosphate receptor 1 enhances olfactory receptor 51E1‑mediated inhibition of proliferation via Src/JNK signaling in prostate cancer cellsOncol RepMarch 19, 2026Kiheon Kim, Jinwoo Lee, Chulwon Choi, Jungnam Bae, Hee-Jung Choi, Won-Ki HuhPromega Corporation (cat. no. E8471). GloSensor-22F plasmid was purchased from Promega Corporation (cat. no. E2301) for measuring cAMP production. For CRISPR-Cas9 gene editing, non-targeting control sgRNA and guide

(2) Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammationCell ReportsJanuary 27, 2026Jun Nagai, Madeline M. Hastings, Airi Nishida, Sreyashi Majumdar, Kendall Zaleski, Sofia A. Marshall, Junrui Lin, Mira A.S. Saliman, Yumi Tani, Yamato Murakami, et al.Muc5acRe:5′-ctcaaggggtgtcagcctaa-3′,IDT,N/A Recombinant DNA,Recombinant DNA,Recombinant DNA GloSensor™ Technology − 22F cAMP plasmid,Promega,E2301 "NanoBiT® PPI Starter Systems, Flexi Starter System",Promega,N2015 pEF1/His vector,ThermoFisher,V922-20 pEF1α-Myc

(3) Activation of the pituitary adenylate cyclase-activating polypeptide type I receptor promotes neuroblastoma proliferation and migration through distinct G protein pathwaysCell Communication and SignalingJanuary 16, 2026Sarah Jang, Jae-Yeon Jeong, Won-Ki Huhplasmid was purchased from Promega (#E1350). The GloSensor-22 F plasmid was purchased from Promega (#E2301) and subcloned into an adenoviral expression vector (#K493000, Invitrogen) for measuring cAMP production

(4) The pathway-independent positive allosteric modulator C1 allows for the identification of active Y4 receptor relevant positionsCellular and Molecular Life SciencesJanuary 13, 2026Corinna Schüß, Oanh Vu, Tim Pelczyk, Mario Schubert, Yu Du, Jan Stichel, C. David Weaver, Jens Meiler, Annette G. Beck-SickingerRenilla luciferase 8 and cloned into the pcDNA3 vector [33, 34]. The pGloSensor-22 F cAMP plasmid (Cat. # E2301) was obtained from Promega (Fitchburg, WI, USA). The G protein sensors (Go1-CASE RRID: Addgene_168123,

(5) Structural insights into selective and dual antagonism of EP2 and EP4 prostaglandin receptorsThe EMBO JournalOctober 29, 2025Yanli Wu, Heng Zhang, Jiuyin Xu, Kai Wu, Wen Hu, Xinheng He, Gaoming Wang, Canrong Wu, H Eric Xustudy,N/A pcDNA3.1 EP2,This study,N/A pcDNA3.1 EP4,This study,N/A GloSensor plasmid,Promega,Cat# E2301 Antibodies,Antibodies,Antibodies Anti-FLAG FITC-conjugated antibody,Sigma,Cat# F4049 Oligonucleotides

(6) Structural insights into the mechanism of activation and inhibition of the prostaglandin D2 receptor 1Nat CommunOctober 8, 2025Behnaz Davoudinasab, Aleksey Raskovalov, Woojin Lee, Donggyun Kim, Heesoo Kim, Jordy Homing Lam, Gye Won Han, Vsevolod Katritch, Vadim Cherezov(New England Biolabs, Cat# B6004S). For cAMP assay, the GloSensor™ Technology -22F cAMP Plasmid (Promega, Cat# E2301) was purchased from Promega. For ONE-GO assay54, the ONE-GO Biosensors Kit was a gift from Mikel Garcia-Marcos

(7) S-nitrosylation of pVHL regulates β 2 adrenergic receptor functionProceedings of the National Academy of SciencesSeptember 16, 2025Zachary W. Grimmett, Hiroki Hayashi, Thomas M. Raffay, Justin Lin, Richard T. Premont, Jonathan S. Stamlerperformed with ImageJ (NIH). cAMP Measurement. cAMP was measured using the GloSensor cAMP assay (#E2301; Promega) as per the manufacturer’s instructions. Briefly, HEK293 cells cultured in 10 cm2 dishes were

(8) A Chemically Stable Photocaged NoradrenalineACS Chem NeurosciAugust 6, 2025Stanley A. Buczynski, Amy Li, Janie Chang-Weinberg, Shayra S. Nawsheen, Matthew R. Banghart(Invitrogen), followed by the addition of the GloSensor cAMP reporter plasmid (−22F cAMP plasmid, Promega, E2301), and for targeting α1-ARs, a plasmid encoding the chimeric Gs/Gq protein sq5 (addgene plasmid #24499)

(9) Orphan receptor GPR153 facilitates vascular damage responses by modulating cAMP levels, YAP/TAZ signaling, and NF-κB activationNat CommunJuly 7, 2025Jingchen Shao, Jeonghyeon Kwon, Tianpeng Wang, Stefan Günther, Lukas S. Tombor, Timothy Warwick, Zaib Shaheryar, Ralf P. Brandes, Stefanie Dimmeler, Jan Wenzel, et al.(Sigma-Aldrich, SIC001). After 24 h, cells were transfected with the GloSensorTM 22 F plasmid (Promega, E2301) and plasmids encoding GPR153 or empty vector (0.1 µg each plasmid). After 16–20 h, cells were trypsinized

(10) Molecular fingerprints of a convergent mechanism orchestrating diverse ligand recognition and species-specific pharmacology at the complement anaphylatoxin receptorsbioRxivMay 30, 2025Sudha Mishra, Manish K. Yadav, Annu Dalal, Manisankar Ganguly, Ravi Yadav, Kazuhiro Sawada, Divyanshu Tiwari, Nabarun Roy, Nilanjana Banerjee, Jenny N. Fung, et al.μg of either hC3aR, mC3aR, hC5aR1, or mC5aR1 along with 3.5 μg of the F22 plasmid (Promega, Cat. No: E2301). After 14–16 h of transfection, cells were trypsinized and resuspended in assay buffer (20 mM HEPES,

(11) Structural basis for regulation of CELSR1 by a compact module in its extracellular regionNat CommunApril 28, 2025Sumit J. Bandekar, Krassimira Garbett, Szymon P. Kordon, Ethan E. Dintzner, Jingxian Li, Tanner Shearer, Richard C. Sando, Demet Araçco-transfected with 0.35 μg CELSR DNA or empty vector, 0.35 μg GloSensor reporter plasmid (Promega, E2301), 9 ng β2 adrenergic receptor, and 2.8 μL of the transfection reagent Fugene 6 (Promega, PRE2693). After

(12) Structural insights into small-molecule agonist recognition and activation of complement receptor C3aRThe EMBO JournalApril 7, 2025Jinuk Kim, Saebom Ko, Chulwon Choi, Jungnam Bae, Hyeonsung Byeon, Chaok Seok, Hee-Jung ChoiGβ1, and Gγ2",This study,N/A pET-21d anti-Fab Nanobody,This study,N/A GloSensor plasmid,Promega,Cat#E2301 Antibodies,Antibodies,Antibodies Rabbit anti-FLAG antibody,Cell Signaling Technology,Cat#14793 Anti-rabbit

(13) Structural visualization of small molecule recognition by CXCR3 uncovers dual-agonism in the CXCR3-CXCR7 systemNat CommunMarch 28, 2025Shirsha Saha, Fumiya K. Sano, Saloni Sharma, Manisankar Ganguly, Annu Dalal, Sudha Mishra, Divyanshu Tiwari, Hiroaki Akasaka, Takaaki A. Kobayashi, Nabarun Roy, et al.cells/well. For GloSensor assay, 1 μg of N-terminally FLAG-tagged receptor and 1 μg of F22 (Promega, Cat. no: E2301) was used to transfect HEK293T cells; for NanoBiT-based G-protein dissociation assay, 0.5 μg of N-terminally

(14) A sensitive biosensor of endogenous Gα i activity enables the accurate characterization of endogenous GPCR agonist responsesScience SignalingMarch 25, 2025Alex Luebbers, Remi Janicot, Jingyi Zhao, Clementine E. Philibert, Mikel Garcia-Marcos(pLVX-CMV-Gαz-V-IRES*-KB1753-Nluc) were described previously (33). The plasmid encoding Glosensor 22F was acquired from Promega (cat#E2301). The plasmid for the expression of the Gαi bONE-GO biosensor (pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc) was

(15) Structural insights into the selective recognition of RF-amide peptides by neuropeptide FF receptor 2EMBO ReportsMarch 24, 2025Jeesoo Kim, Sooyoung Hong, Hajin Lee, Hyun Sik Lee, Chaehee Park, Jinuk Kim, Wonpil Im, Hee-Jung ChoiGβ1, and Gγ2",This study,N/A pET-21d anti-Fab Nanobody,This study,N/A GloSensor plasmid,Promega,Cat#E2301 Antibodies,Antibodies,Antibodies Rabbit anti-FLAG antibody,Cell Signaling Technology,Cat#14793 Anti-rabbit

(16) Molecular basis of promiscuous chemokine binding and structural mimicry at the C-X-C chemokine receptor, CXCR2Molecular CellMarch 6, 2025Shirsha Saha, Fumiya K. Sano, Saloni Sharma, Manisankar Ganguly, Sudha Mishra, Annu Dalal, Hiroaki Akasaka, Takaaki A. Kobayashi, Nashrah Zaidi, Divyanshu Tiwari, et al.described previously.67–69 HEK293T cells were transfected with a mixture of 3.5μg of F22 (Promega, Cat. no: E2301) and either 3.5μg of CXCR2WT or 3.5μg of CXCR2RRR or 3μg of CXCR2DD, harboring an N-terminal FLAG tag.

(17) Orphan G protein-coupled receptor GPRC5B controls macrophage function by facilitating prostaglandin E receptor 2 signalingNat CommunFebruary 7, 2025Jeonghyeon Kwon, Haruya Kawase, Kenny Mattonet, Stefan Guenther, Lisa Hahnefeld, Jamal Shamsara, Jan Heering, Michael Kurz, Sina Kirchhofer, Cornelius Krasel, et al.GPRC5B (0.1 µg), the indicated prostanoid receptors (0.1 µg), and the GloSensor 22 F (0.1 µg, Promega, E2301). After 24 h, 100 μl of cells (4 × 105 cell/ml) were transferred to a 96 well plate. The next day, the

(18) Molecular basis of proton sensing by G protein-coupled receptorsCellFebruary 6, 2025Matthew K. Howard, Nicholas Hoppe, Xi-Ping Huang, Darko Mitrovic, Christian B. Billesbølle, Christian B. Macdonald, Eshan Mehrotra, Patrick Rockefeller Rockefeller Grimes, Donovan D. Trinidad, Lucie Delemotte, et al.Research,Cat# D4074 Q5 Site-Directed Mutagenesis Kit,NEB,Cat# E0554S GloSensor cAMP Assay,Promega,Cat# E2301 ,, Deposited data,Deposited data,Deposited data ,, GPR68 deep mutational scan fitness scores,This

(19) Insurmountable antagonism of human mu opioid receptors by buprenorphine is due to hemi-equilibriumEuropean Journal of PharmacologyJanuary 15, 2025Michael J. Wedemeyer, Teresa S. Chavera, Kelly A. Berg, William P. Clarke(# 100–508). The D-Luciferin was purchased from GoldBio (#eLUCK), GloSensor-22F was from Promega (#E2301), and Hygromycin B was from Roache (#843555). 2.2. Cell culture HEK-293 cells stably expressing the

(20) Conformational coupling between extracellular and transmembrane domains modulates holo-adhesion GPCR functionNature CommunicationsDecember 4, 2024Szymon P. Kordon, Kristina Cechova, Sumit J. Bandekar, Katherine Leon, Przemysław Dutka, Gracie Siffer, Anthony A. Kossiakoff, Reza Vafabakhsh, Demet Araç50–60% confluence, they were transfected with 350 ng of ADGRL3 + 350 ng 22 F Glosensor reporter plasmid (E2301; Promega) + 9 ng of β2-adrenergic receptor + 2.8 uL of transfection reagent Fugene 6 (Promega, PRE2693).

Be the First to Write a Review!

pGloSensor-22F cAMP Plasmid from Promega

Description

Specifications/Features

Promega

Citations (67)

Reviews

pGloSensor-22F cAMP Plasmid from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map