Transcend tRNA from Promega

Product Specs
ItemTranscend tRNA
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL5061
Quantity30microl
Molecular Weight~108kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Feeding-regulated glycogen metabolism drives rhythmic liver protein secretionNature MetabolismFebruary 5, 2026Meltem Weger, Daniel Mauvoisin, Dominic Hoyle, Jingkui Wang, Eva Martin, James Rae, Charles Ferguson, Glynis Klinke, Michelle Cielesh, Kyle L. Macauslane, et al.SV40 polyadenylation signal (VectorBuilder, T7::Serpina1d_SV40polyA) and biotinylated tRNA (Promega, L5061) at 30 °C for 90 min. The translation mix was combined 1:1 (v/v) with serum and incubated at 37 °C for

(2) Deleterious Effects of Histidine Tagging to the SH3b Cell Wall-Binding Domain on Recombinant Endolysin ActivityJournal of Microbiology and BiotechnologyNovember 28, 2024Jin-Mi Park, Jun-Hyun Kim, Kang-Seuk Choi, Hyuk-Joon Kwondistilled water to the protein synthesis mixture, we used 3 μl of Transcend tRNA (Promega Corp., USA; L5061). For western blot analysis, 10 μl of the protein supernatant was mixed with 50 μl of acetone and incubated

(3) A novel long noncoding RNA AK029592 contributes to thermogenic adipocyte differentiationStem Cells Translational MedicineOctober 10, 2024Pengyu Hong, Dianri Wang, Yue Wu, Qi Zhang, Pan Liu, Jian Pan, Mei Yu, Weidong TianIn vitro transcription/translation assay was performed using a kit from Promega (L1171, Promega) and Transcend tRNA (L5061, Promega). Briefly, expression plasmids for luciferase and lncRNA AK029592 were mixed with a Coupled

(4) eIF4B mRNA Translation Contributes to Cleavage Dynamics in Early Sea Urchin EmbryosBiology (Basel)September 27, 2022Florian Pontheaux, Sandrine Boulben, HÃ©loÃ¯se ChassÃ©, AgnÃ¨s Boutet, Fernando Roch, Julia Morales, Patrick Cormieranalysis. Biotinylated P. lividus eIF4B protein was detected using streptavidin-HRP (1/10,000) (Promega-L5061). eIF4E was revealed with a specific primary antibody (1/5000) and a horseradish peroxidase-coupled

(5) An AMPK phosphoregulated RhoGEF feedback loop tunes cortical flow-driven amoeboid migration in vivoScience advancesSeptember 16, 2022Lin B, Luo J, Lehmann R.TNT T7 Quick Coupled Transcription/Translation System (Promega, L1170) with Transcend tRNA (Promega, L5061) was used to in vitro translate the RhoGEF2 PH domain according to the manufacturer’s recommendation. Coimmunoprecipitation S2

(6) RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start siteCell reportsNovember 2, 2021George J, Li Y, Kadamberi IP, Parashar D, Tsaih SW, Gupta P, Geethadevi A, Chen C, Ghosh C, Sun Y, et al.deoxycholate,Sigma Aldrich,Cat# D6750 Sucrose,Sigma Aldrich,Cat# S7903 Transcend (TM) tRNA,Promega,Cat# L5061 Triton X-100,Sigma Aldrich,Cat# T8787 Trypsin,Thermo Fisher Scientific,Cat# 15400054 Tween 20,Sigma Aldrich

(7) Revealing protein-protein interactions at the transcriptome scale by sequencingMolecular cellOctober 7, 2021Johnson KL, Qi Z, Yan Z, Wen X, Nguyen TC, Zaleta-Rivera K, Chen CJ, Fan X, Sriram K, Wan X, et al.Display Protein products were generated using 25 pmols of RNA product and 2 uLs Transcend™ tRNA (Promega, L5061) per 25 uL reaction of the NEB PURExpress IVT kit. 2 uLs of Proteinase K was added to the “protein digested

(8) A small molecule that induces translational readthrough of CFTR nonsense mutations by eRF1 depletionNature communicationsJuly 16, 2021Sharma J, Du M, Wong E, Mutyam V, Li Y, Chen J, Wangen J, Thrasher K, Fu L, Peng N, et al.while a 35 kDa protein was generated by readthrough of the PTC. The Transcend™ tRNA (Promega, cat.# L5061) was added to the system for the chemiluminescent detection of the protein products via incorporation

(9) mRNA-based SARS-CoV-2 vaccine candidate CVnCoV induces high levels of virus-neutralising antibodies and mediates protection in rodentsNPJ vaccinesApril 16, 2021Rauch S, Roth N, Schwendt K, Fotin-Mleczek M, Mueller SO, Petsch B.incubated with Rabbit Reticulate Lysate, Amino Acid Mixtures, Transcend™ Biotin-Lysyl-tRNA (Promega, Cat.L5061), RNasin® Ribonuclease Inhibitors (Promega, Cat. N2511) and Nuclease-Free water in a total volume of

(10) Transcriptional control of a novel long noncoding RNA Mymsl in smooth muscle cells by a single Cis-element and its initial functional characterization in vesselsJournal of Molecular and Cellular CardiologyJanuary 1, 2020Mihyun Choi, Yao Wei Lu, Jinjing Zhao, Mingfu Wu, Wei Zhang, Xiaochun LongTranscription/Translation System (Promega, cat# L1171) and Transcend Biotin-Lysyl-tRNA label system (Promega, #L5061) as described [30]. Briefly, 0.5 μg of each plasmid was mixed with TNT T7 Quick Master Mix, Methionine,

(11) Selective degradation of PU.1 during autophagy represses the differentiation and antitumour activity of T_H9 cellsNature communicationsSeptember 15, 2017Rivera Vargas T, Cai Z, Shen Y, Dosset M, Benoit-Lizon I, Martin T, Roussey A, Flavell RA, Ghiringhelli F, Apetoh L.Transcription/Translation Systems (Promega L1170) combined with biotinylated lysine (Transcend tRNA Promega L5061) according to the manufacturer’s instructions. These two proteins were subsequently incubated with total

(12) ATF4 promotes bone angiogenesis by increasing VEGF expression and release in the bone environmentJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchSeptember 1, 2013Zhu K, Jiao H, Li S, Cao H, Galson DL, Zhao Z, Zhao X, Lai Y, Fan J, Im HJ, et al.using the Rabbit Reticulocyte Lysate System (Promega, L4960) and Biotinylated Lysine-tRNA (Promega, L5061) following manufacturer's instructions. Two μl translated protein products were subject to SDS-PAGE,

(13) Shp2 controls female body weight and energy balance by integrating leptin and estrogen signalsMolecular and cellular biologyMay 1, 2012He Z, Zhang SS, Meng Q, Li S, Zhu HH, Raquil MA, Alderson N, Zhang H, Wu J, Rui L, et al.express protein by TNT coupled reticulocyte lysate systems (cell-free protein expression, catalog nos. L5061 and L5020; Promega). The translation products were mixed with or without estrodial17␤ and subjected

(14) Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vectorPloS oneJanuary 1, 2011Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD.TnT T7 Coupled Reticulocyte Lysate System (Promega L4610) in the presence of biotinylated lysine (Promega L5061). 5 µL of each TnT-generated protein sample was resolved via PAGE, transferred to PVDF membrane, incubated

(15) Heterogeneous nuclear ribonucleoprotein (hnRNP) E1 binds to hnRNP A2 and inhibits translation of A2 response element mRNAsMolecular biology of the cellAugust 1, 2006Kosturko LD, Maggipinto MJ, Korza G, Lee JW, Carson JH, Barbarese E.described above. RNA (150 ng/reaction) was translated using the Flexi RRL (#L4540) and Transcend tRNA (#L5061) from Promega (Madison, WI) according to the manufacturer’s instructions. In some experiments, the translation

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Transcend tRNA

Transcend tRNA from Promega

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Transcend tRNA from Promega

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