LongAmpTaqDNA Polymerase from New England Biolabs

Product Specs
ItemLongAmpTaqDNA Polymerase
CompanyNew England Biolabs
Price $93.00 Inquire
Catalog NumberM0323S
Concentration2,500 units/ml
Quantity500 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Buffer:
1X LongAmp™ Taq Reaction Buffer

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U-87 MG cellsFEBS Open BioAugust 13, 2026Pragya Gupta, Sebastian Gb Furness, Tahereh Gharbi, Ric De Paoli-Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookeybarcoding. One nanogram of each DNA sample was uniquely barcoded using Long‐Amp Taq polymerase (no.: M0323S; New England Biolabs, Ipswich, Massachusetts, USA) and barcoding kit (no.: EXP‐PBC096; ONT) as per the

(2) Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement via Single-Cell GenomicsbioRxivJune 1, 2026Federica Sozza, Alberto Romano, Nicole D'Elia, Martina Terenzi, Maria Luisa Ratto, Emily R. Cliff, Gabrielle Nattenberg, Sara Bianchi, Silvia Becca, Heather Klug, et al.D5000,Agilent,Cat# 5067-5588 In-Fusion Snap Assembly,Takara,Cat# 638943 LongAmp Taq DNA Polymerase,NEB,Cat# M0323L NEBNext High-Fidelity 2X PCR Master Mix,NEB,Cat# M0541S NEBuilder HiFi DNA Assembly Mix,NEB,Cat# E5520S PaqCI

(3) Epigenetic phase variation in the gut microbiome enhances bacterial adaptationCell Host MicrobeMay 19, 2026Mi Ni, Katerina Junker, Yujie Liu, Yu Fan, Yangmei Li, Wanjin Qiao, Xue-Song Zhang, Magdalena Ksiezarek, Edward A. Mead, Alan Tourancheau, et al.cocktail,Sigma-Aldrich,Cat#P8340 T7 Endonuclease I,NEB,Cat#M0302S LongAmp Taq DNA Polymerase,NEB,Cat#M0323 β-Mercaptoethanol,Sigma-Aldrich,Cat#M3148 AMX test strips (ETEST),Liofilchem / AES,Cat# 22-777-711 Chloramphenicol,Fisher

(4) Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variationCell Reports MethodsMay 18, 2026Nick Vereecke, Megan Behrmann, Anupama Khare, John P. Dekkerrecombinant proteins","Chemicals, peptides, and recombinant proteins" ,, LongAmp Taq Master Mix (2X),NEB,M0323S AMPure XP beads,Beckman Coulter,A63881 EB Ultra-II End-prep Enzyme Mix,NEB,E7546S Long-Fragment Buffer

(5) Assessment of nanopore RNA modification calling in human cell lines and synthetic systemsGenome BiologyMay 7, 2026Neda Ghohabi Esfahani, Andrew J. Stein, Stuart Akeson, Talia Tzadikario, Connor Powell, Pooria Daneshvar Kakhaki, Miten Jainreaction mixture included 1 μL of 10 mM DNA template, 1 μL of 10 mM dT Primer, 12.5 2 × Long AMP Taq (NEB #M0323), and 10.5 μL of RNase-free water, which were combined in a separate tube for each template. Using the

(6) Comparative analysis of draper mutant alleles and RNAi expression systems in the ovary and brain of Drosophila melanogasterG3: Genes, Genomes, GeneticsMay 6, 2026Guangmei Liu, Pamela Yang, Trung Le, Jerald Shin, Cheng Yang Shi, Emily Budhram, Kimberly McCalltranscription-polymerase chain reaction (RT-PCR) and qRT-PCR. The LongAmplification Taq polymerase kit (NEB #M0323S) was used to perform RT-PCR. In nuclease-free tubes on ice, samples were prepared for each genotype

(7) Engulfment by brain macrophages in a short-lived vertebratebioRxivMay 5, 2026Rahul Nagvekar, Angela N. Pogson, Prateek R. Kalakuntla, Helena J. Barr, Azalia M. Martínez Jaimes, S.V. Perry, Emma K. Costa, Jingxun Chen, Felix Boos, Paloma Navarro Negredo, et al.1 μL (<200 ng) of DNA was used as input in a PCR with 1 μL LongAmp polymerase (New England Biolabs, M0323S), 5 µL 5x LongAmp Taq Reaction Buffer (New England Biolabs, M0323S), 3.75 μL 2 mM DNTPs (e.g., Sigma-Aldrich,

(8) Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contextsmedRxivApril 24, 2026Shawn Fayer, Riddhiman K. Garge, Melissa Hopkins, Clayton E. Friedman, Abby V. McGee, Joshua Rico, Rachel L. Powell, Evan McDermot, Nahum T. Smith, Sriram Pendyala, et al.cat. no. M0494S). POLG allele specific PCR was performed with LongAmp Taq DNA Polymerase (NEB; cat. no.M0323S). gDNA PCRs were split into 4 reactions with 250 ng gDNA each and pooled for AMPure XP Bead (Beckman Coulter

(9) Evaluation of Nanopore direct RNA sequencing updates for modification detectionbioRxivApril 12, 2026Neda Ghohabi Esfahani, Andrew J Stein, Stuart Akeson, Talia Tzadikario, Miten Jainreaction mixture included 1 μL of 10 mM DNA template, 1 μL of 10 mM dT Primer, 12.5 2x Long AMP Taq (NEB #M0323), and 10.5 μL of RNase-free water, which were combined in a separate tube for each template. Using the

(10) Defective Trophoblast Differentiation, Endothelial Dysfunction, and Immune Dysregulation in Preeclampsia Coalesce on a Placental VGLL3-Centered Gene NetworkCirculationApril 9, 2026Olesya Plazyo, Laura B Chopp, Rishyanth Peela, Kelly Young, Haihan Zhang, Rachael Bogle, Ashley Hesson, Elizabeth S Langen, Ingrid L Bergin, Li-Jyun Syu, et al.validated by analyzing high molecular weight genomic DNAs extracted from F1 pups with long range PCR (NEB# M0323S LongAmp Tag DNA polymerase), to confirm proper 5’ and 3’ integration into the Rosa26 locus. 2058 bp

(11) Defective trophoblast differentiation, endothelial dysfunction, and immune dysregulation in preeclampsia coalesce on placental VGLL3-centered gene networkCirculationApril 9, 2026Olesya Plazyo, Laura B Chopp, Rishyanth Peela, Kelly Young, Haihan Zhang, Rachael Bogle, Ashley Hesson, Elizabeth S Langen, Ingrid L Bergin, Li-Jyun Syu, et al.validated by analyzing high molecular weight genomic DNAs extracted from F1 pups with long range PCR (NEB# M0323S LongAmp Tag DNA polymerase), to confirm proper 5’ and 3’ integration into the Rosa26 locus. 2058 bp

(12) Oxford Nanopore enhanced accuracy of long-read amplicons applied to microbial whole-genome sequencingMicrobiology SpectrumApril 7, 2026Marion Helsmoortel, Erwin Sentausa, Adrien Villain, Viet-Dung Tran, Emmanuelle Santiago-Allexant, Corinne Beaulieu, Amy Hesketh, Josephine Abi-Ghanem, Philippe Leissner, Adrien SaliouKapa HiFi Hot Start DNA polymerase (07958897001, Roche Diagnostics), and LongAmp Taq DNA polymerase (M0323, New England Biolabs). A range of DNA inputs was tested, from 0.1 to 100 pg added in the first PCR.

(13) Neuron-derived mitochondrial DNA (mtDNA) activates microglia via the Z-DNA binding protein 1 (ZBP1)-mediated pathway in mild traumatic brain injuryProc Natl Acad Sci U S AApril 7, 2026Michela Marcatti, Javier Allende Labastida, Tony Zifeng Tang, Akbar Ahmad, Christina Payne, Nora Schwartz, Paula Villarreal, Olivia D. Solomon, Gracie Vargas, Ping Wu, et al.kb for rat DNA. Taq DNA Polymerase and LongAmp Taq DNA Polymerase (New England BioLabs #M0273X and #M0323L) were used to amplify short and long DNA targets, respectively. The number of cycles was determined

(14) Primary ciliogenesis is promoted during epithelial-mesenchymal transition via a miR-200 – DZIP1 axisCell Communication and SignalingApril 1, 2026Laura Sourdin, Julie M. Bracken, Katherine A. Pillman, Andrew G. Bert, Yeesim Khew Goodall, Philip A. Gregory, Gregory J. Goodall, Cameron P. BrackenPrimers are listed in Supplementary Table 4. DNA was amplified using LongAmp DNA Polymerase (NEB, #M0323), extracted from agarose gels (Isolate-II Gel Purification Kit, Bioline, #Bio-52060), digested with

(15) Temperature and genetic background drive mobilization of diverse transposable elements in a global human fungal pathogenPLOS GeneticsJanuary 16, 2026Anna I. Mackey, Vesper Fraunfelter, Samantha Shaltz, John McCormick, Callan Schroeder, John R. Perfect, Cedric Feschotte, Paul M. Magwene, Asiya Gusa(ThermoFisher #12368010), Q5 High-Fidelity DNA Polymerase (NEB #M0491S), and LongAmp Taq DNA Polymerase (NEB #M0323S). We often observed “phantom” wild-type–sized secondary bands when amplifying across TEs, which can

(16) INSIG1 parallel substitution drives lipid/sterol metabolic plasticity mediating desert adaptation in ungulatesCommunications BiologyJanuary 12, 2026Xinmei Li, Ziyi He, Anguo Liu, Fanxin Meng, Xiao Zhang, Nana Li, Huan Liu, Yuyi Lu, Zhipei Wu, Huimei Fan, et al.5′-TGCCGAGGAAAATAAGTGGTTTGG-3′) and R1 (reverse, 5′-AGCCACAGTAAACCTCTGCTTCTA-3′), using LongAmp Taq DNA polymerase (NEB M0323L) for 33 cycles at an annealing temperature of 60 °C, which yielded a 403-bp amplicon. A no-template

(17) Telomerase mRNA therapy protects human skin against radiation-induced DNA damageMolecular TherapyJanuary 7, 2026Shuang Li, David F. Chang, Karem A. Court, Thi Kim Cuc Nguyen, Vrutant V. Shah, Elisa Morales, Jack Carrier, Anjana Tiwari, Andrew T. Ludlow, Kristopher W. Brannan, et al.previously.58 Briefly, 10 ng DNA from each sample was amplified with LongAmp Taq DNA Polymerase (NEB, no. M0323) for genomic and mitochondrial DNA amplicons, using the primer sequences and PCR conditions given in

(18) Recombination junctions from antibody isotype switching classify immune and DNA repair dysfunctionNat CommunDecember 19, 2025Clara Vázquez García, Benedikt Obermayer, Baerbel Keller, Mikhail Lebedin, Christoph Ratswohl, Hassan Abolhassani, Antonia Busse, Michela Di Virgilio, Stephan Mathas, Dorothee Speiser, et al.3 μl mM dNTPs each (Roboklon, #E0501-01), 5 μl LongAmpTaq Buffer, 1 μl LongAmpTaq polymerase (NEB, #M0323), nuclease-free water, primers and template until a total volume of 25 μl, 1 μl of 10 μM Sμ FW, Sα RV

(19) Single cell transcriptional perturbome in pluripotent stem cell modelsMolecular Systems BiologyDecember 10, 2025Elisa Balmas, Maria L Ratto, Kirsten E Snijders, Silvia Becca, Carla Liaci, Irene Ricca, Giorgio R Merlo, Raffaele A Calogero, Luca Alessandrì, Sasha Mendjan, et al.mg/mL),Gibco,#10131035 Fialuridine,Sigma-Aldrich,#SML0632 LongAmp Taq DNA Polymerase (2.5 U/µL),New England Biolabs,#M0323S LongAmp Taq Reaction Buffer (5X),New England Biolabs,#B0323S Paraformaldehyde 4% (PFA),Electron Microscopy

(20) Efficacy of Minnelide in a Next-Generation Dual-Recombinase Regulated Genetically Engineered Mouse Model of CIC::DUX4 SarcomabioRxivNovember 8, 2025MaKenna R. Browne, Axel V. Silver, Risha Banerjee, Benigno Aquino, Kristianne M. Oristian, Jonathon E. Himes, Peter G. Hendrickson, David G. Kirschthe unrecombined large amplicon size, PCR was performed using NEB LongAmp Taq DNA Polymerase (Cat. M0323S). dFLEx CDS tumor formation and evaluation of Minnelide in vivo 6–12 week old dFLEx CDS mice were

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