ReliaPrep RNA Cell Miniprep System from Promega

Product Specs
ItemReliaPrep RNA Cell Miniprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ6010
Quantity10 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeCultured cells or tissue

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protocol for simultaneous profiling of transcription start sites and full-length transcripts from low-input samples using Smart-seq+5′STAR ProtocolsJune 19, 2026Diego Rodriguez-Terrones, Marlies E. Oomen, Maria-Elena Torres-Padillathe extracted RNA in nuclease-free water. NOTE: We have successfully used the Reliaprep kit (Promega #Z6011) for this step, however any RNA extraction method that results in pure and high integrity RNA can be

(2) Status Epilepticus Alters the Function of Brain-Derived Extracellular VesiclesJ Extracell VesiclesJune 17, 2026Samantha L. Reed, Yilin Wang, Yangping Li, Guoku Hu, Jingjing Yang, Bing Yao, Andrew EscaygBDEV‐treatment using the ReliaPrep RNA Cell Miniprep System per manufacturer's instructions (Promega Z6011). Samples were resuspended in 30 µL of RNase‐free water and stored at −80°C until further use. BDEV

(3) Pancreatic cancer induces B cell lineage plasticity via Pax5 inhibition to sustain immunosuppressionCell Death DiscovJune 2, 2026Ali Kassem, Nataly Naser Al Deen, Sun Yifeng, Chau Fang, Laura Mayer, Lingling Zhang, Paul Kunath, Thomas Wirth, Uwe Knippschild, Mohammad Rahbari, et al.16 h or 5 days. RNA was extracted by using the ReliaPrep RNA Cell Miniprep System (Promega, Cat. No. Z6012) according to instructions provided by the manufacturer. Residual genomic DNA was digested using DNase I

(4) Food-Derived Elastin Peptides Improve Glucose Metabolism and Protect Renal Vasculature in Stroke-Prone Spontaneously Hypertensive Rats Despite Modest Dipeptidyl Peptidase 4 InhibitionNutrientsMay 30, 2026Kumiko Takemori, Yuki Nakamura, Kenji Sato, Eri Shiratsuchi, Takashi Kometani, Seiji Masudaextracted from both the glomerular and LRFs using the ReliaPrep™ RNA Cell Miniprep System (Promega, Z6010, Madison, WI, USA). Sample optical density was measured at 230, 260, and 280 nm using an e-Spect ES2

(5) Inhibition of RNA polymerase II-activating CDK9 and CDK12/13, but not of cell cycle relevant CDKs, induces apoptosis by downregulating the short-lived Bcl-2 proteins Mcl1 and Bfl1/A1Cell Death DisMay 27, 2026Karina S. Krings, Judith Hatzfeld, Sandra Weller, Nadine Borkowski, Tanya R. Llewellyn, Laura Schmitt, Bo Scherer, Harri M. Itkonen, Christoph Peter, Björn Stork, et al.transcription quantitative PCR Total RNA extraction was performed using the ReliaPrep™ RNA Miniprep Kit (#Z6012, Promega). One microgram (1 µg) of RNA was reverse transcribed into cDNA using M-MLV Reverse Transcriptase

(6) Ultrasound modulates microglial activity and reduces neuroinflammation in a parameter-dependent mannerNPJ AcoustMay 6, 2026Sarina Grewal, Francesco Iacoponi, Lok Yin Nicholas Chan, Valeria Dosso, William Lim Kee Chang, Vanessa Drevenakova, Albert Ugwudike, Leonardo Ricotti, Paul M. Matthews, Andrea Cafarelli, et al.ReliaPrep™ RNA Cell Miniprep System according to the manufacturer’s instructions at 24, 48 and 72 h post-ultrasound (Promega, Cat. Z6011). Next, a NanoDrop spectrophotometer was used to assess the concentration and the purity of the RNA.

(7) A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal TransitionbioRxivApril 20, 2026Weimin Liu, Yusof Gusti, Fathima Athar, Naresh K Rajendran, Paul A. Cahill, Eileen M. Redmondalternate day. Real time qPCR. Total RNA was isolated using ReliaPrep TM RNA cell Miniprep System (Cat. # Z6011, Promega) following manufacturer’s instructions. DNase-treated RNA was either used in one-step qRT-PCR

(8) Transient remodeling of gut metabolism supports juvenile growth and adult fitness in DrosophilaNat CommunApril 13, 2026Clara Lefranc, Arnaud Fichant, Gilles Storellifurther processing. Total RNA was extracted using the Reliaprep RNA Tissue Miniprep System (Promega, Cat#Z6012). 500 µL of lysis buffer was added to the microtubes and tissues were homogenized using a Precellys

(9) Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary FibroblastsbioRxivApril 5, 2026Nicolas P. Holcomb, Ronel Z. Samuel, Alena Klochkova, Joanna Wong, Sara Kass-Gergi, Meryl Mendoza, Michael M. Maiden, Evelyn A. Martinez, Diana M. Abraham, Madeline Singh, et al.Isolation and RT-qPCR: RNA was isolated using the either the ReliaPrep™ RNA Cell Miniprep kit (Promega, Z6011) or Direct-zol RNA Miniprep Plus (ZymoResearch; R2072) and concentration/quality was assessed via nanodrop.

(10) Chromatin dynamics of the Klf4 locus in mouse pluripotent cellsScientific ReportsMarch 27, 2026Jente van Staalduinen, Hélène Kabbech, Selçuk Yavuz, Ridvan Cetin, Agnese Loda, Wiggert van Cappellen, Adriaan Houtsmuller, Kerstin Wendt, Ihor Smal, Frank Grosveldnaïve and differentiation conditions with the ReliaPrep RNA Cell Miniprep system (Promega, Catalog no: Z6012) according to manufacturer’s instructions. After RNA isolation, RNA was treated with 1 μL TURBO DNase

(11) IGFBP3 repression driven by inflammation links air pollution to placental and developmental defectsEMBO Molecular MedicineMarch 24, 2026Sunil Singh, Isha Goel, Anubhuti Rana, Anamta Gul, Javed A Quadri, Asit Ranjan Mridha, Lakshay Malhotra, Neha Kashyap, Baburajan Radha, Arnab Nayek, et al.Cell Assay Kit,Himedia Laboratories Private Ltd.,CCK032 Total RNA isolation kit,Promega Corporation,Z6011 Nanodrop,"Thermo Fisher Scientific, Inc", Verso cDNA synthesis Kit,"Thermo Fisher Scientific, Inc.",AB1453A DyNAmo

(12) Long-term exposure to nanoparticles alters senescence-associated markers and immune responses in human monocyte-derived macrophagesNanoscaleMarch 18, 2026Henry Lee, Moritz Häffner, Sandor Balog, Patricia Taladriz-Blanco, Aurélien Mroczek, Simona Staudinger, Alke Petri-Fink, Barbara Rothen-RutishauserMadison, WI, USA), and total RNA was extracted using the ReliaPrep™ RNA Cell Miniprep System (Promega, Z6012, Madison, WI, USA). RNA concentration and quality were verified using a NanoDrop™ 2000 spectrophotometer

(13) ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage-like foam cells in atherosclerosisCardiovascular ResearchMarch 16, 2026Bryce R Evans, Julia Schulz, Vasiliki Triantafyllidou, Anais Yerly, Manovriti Thakur, Nico Angliker, Mark Siegrist, Yvonne Jansen, Yi Yan, Sanne L Maas, et al.α-NETA or 100 nM C9, which was maintained overnight. RNA was isolated using the Promega RNA Miniprep (Z6011) Systems and quantified using a NanoDrop™ 2000/2000c spectrophotometer (Thermo Fisher, Waltham, MA,

(14) Insights from pooled CRISPRi single-cell screens in K562 cells reveal gene functions, regulatory networks, and highlight opportunities and limitationsBMC GenomicsMarch 13, 2026Hong Zhang, Peifen Zhang, Eric Bindels, Eskeatnaf Mulugetaextraction. RT-qPCR Total RNA was isolated using ReliaPrep™ RNA Cell Miniprep System (Promega, catalog no. Z6012) and converted to cDNA using SuperScipt IV Reverse Transcriptase kit (Invitrogen, catalog no. 18090200)

(15) RNA binding protein Sam68 promotes germinal center reaction and IgG response through regulation of miR29Cell Mol Life SciMarch 7, 2026Moumita Datta, Valerio Renna, Manish Kumar, Palash Chandra Maity, Hassan Jumaadescribed previously. RNA was isolated from the sorted cells using ReliaPrep Cell RNA miniprep kit (#Z6011, Promega). Fifty nanogram of total RNA was reverse transcribed using high-capacity RNA to cDNA kit (#4387406,

(16) Nppa and Nppb Deficiency Drives Ventricular Hypertrophy and Subendocardial Gene Deregulation in the Mouse HeartInt J Mol SciMarch 6, 2026Alexandra E. Giovou, Otto J. Mulleners, Marie Günthel, Joyce C. K. Man, Bjarke Jensen, Monika M. Gladka, Vincent M. ChristoffelsqPCR Total RNA was isolated from mouse left ventricles using ReliaPrep™ RNA Miniprep Systems (Promega, #Z6012, Madison, WI, USA) according to the manufacturer’s protocol. 1 μg of total RNA was reverse transcribed

(17) Postbiotic Metabolites from a 31-Strain Lactobacillus/Bifidobacterium Co-Culture Attenuate DSS Colitis with Barrier- and Circadian-Linked Transcriptomic SignaturesBiology (Basel)March 5, 2026Shuhei Ueda, Takumi Iwasawa, Kaho Ohki, Satoshi Takeda, Ryohma Tsuchiya, Shunsuke Sakuraba, Kazunori Kato, Tomoaki ItoQuantitative PCR (qPCR) Total RNA was extracted using ReliaPrep™ RNA Miniprep Systems (Promega, Madison, WI, USA; Z6010 according to the manufacturer’s protocol). RNA was quantified using DeNovix DS-11 FX. Reverse transcription

(18) Alignment of in vitro and in vivo pulmonary inflammation models using crystalline quartz silicaParticle and Fibre ToxicologyFebruary 19, 2026Isidora Loncarevic, Seyran Mutlu, Martina Dzepic, Sandeep Keshavan, Sandor Balog, Alke Petri-Fink, Fabian Blank, Barbara Rothen-Rutishauserwas performed directly on the insert, using 100 µL of BL + TG buffer (Promega Madison, WI, USA, Ref. Z6011), and total RNA was extracted using ReliaPrep™ RNA Cell Miniprep System (Promega, Madison, WI, USA,

(19) Electrolyzed Hydrogen Water Improves Chemosensitivity to Anticancer Drugs by Potently Suppressing AutophagyJournal of Cellular and Molecular MedicineFebruary 17, 2026Satoshi Yano, Liangjing Xie, Jinjuan Li, Yuka Sugaya, Yuki Miyauchi, Shigeru Kabayama, Taichi Harah. Total RNA was isolated using the ReliaPrep RNA Cell Miniprep Systems (Promega, Madison, WI, USA; Z6012), following the manufacturer's instructions. Reverse transcription of RNA to cDNA was performed using

(20) Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylationNucleic Acids ResFebruary 12, 2026Marika Oksanen, Francesca Mastropasqua, Krystyna Mazan-Mamczarz, Jennifer L Martindale, Xuan Ye, Abishek Arora, Nirad Banskota, Myriam Gorospe, Kristiina Tammimiesextraction columns, and the final extraction was carried out with ReliaPrep RNA Cell Miniprep kit (Promega Z6012). The RNA was reverse transcribed using iScript complementary DNA (cDNA) Synthesis Kit (BioRad) and

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