ReliaPrep RNA Cell Miniprep System from Promega

Product Specs
ItemReliaPrep RNA Cell Miniprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ6012
Quantity250 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeCultured cells or tissue

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Temporal single-cell transcriptional dynamics of murine pancreatic islet remodeling during hyperglycaemia progressionMolecular MetabolismAugust 1, 2026Simran Singh, Musale Krushna Pavan, Luiz F. Barella, Jayesh Telang, Ajita Shree, Shruti Agarwal, Ayush Goel, Saahiba Thaleshwari, Jürgen Wess, Hamim Zafar, et al.assays,Critical commercial assays,Critical commercial assays ,, ReliaPrep™ RNA Miniprep Systems,Promega,Cat#Z6010 PrimeScript 1st strand cDNA Synthesis Kit,Takara,Cat# 6110A TB Green Premix Ex Taq II (Tli RNase H

(2) Scalable human neuronal models of tauopathy producing endogenous seed-competent 4R tauSci AdvJuly 31, 2026Eliona Tsefou, Sumi Bez, Timothy J. Y. Birkle, Martha Foiani, Naoto Watamura, Mathieu Bourdenx, Daria Gavriouchkina, Emir Turkes, Samuel Crawford, Rachel Coneys, et al.were stored at −70°C. RNA extraction was performed using ReliaPrep RNA Cell Miniprep System (Promega; Z6012) according to the manufacturer’s instructions. Reverse transcription was performed by using 300 ng RNA

(3) The chicken retrovirus-like gene ENS-1/ERNI and Soprano LTR are involved in primordial germ cell developmentiScienceJuly 17, 2026Yuya Okuzaki, Akane Kawaguchi, Yumi Ozaki, Takeo Uemura, Daisuke Saito, Ken-ichi Nishijima2,Kaneka,Cat#KN-B101005 MitoPT JC-1 Assay kit,ImmunoChemistry Technologies,Cat#924 ReliaPrep RNA Miniprep Systems,Promega,Cat#Z6010 Nano-Glo Dual-Luciferase reporter assay system,Promega,Cat#N1620 DNeasy Blood & Tissue Kit,Qiagen,Cat#69506 ,, Deposited

(4) Effects of Budesonide on Coronavirus-Associated Receptor and Immune-Mediator Expression in Human Lung Microvascular Endothelial CellsMedicina (Kaunas)July 12, 2026Izabela Ławska, Adrian Bekier, Maciej Chałubiński, Aleksandra WardzyńskaReal-Time Polymerase Chain Reaction (RT-PCR) mRNA was extracted from HMVEC-L cells using the ReliaPrep RNA Cell Miniprep System (Z6012, Promega, Madison, WI, USA) according to the manufacturer’s instructions. Subsequently, cDNA was synthesized

(5) Modelling DMC1 mediated homologous recombination repair in mouse embryonic stem cellsFront Cell Dev BiolJuly 3, 2026Aditya Mhaskar, Esther Sleddens-Linkels, Ivy Liang, Fiona Horne, Achmed Al-Kourdi, Johan A. Slotman, Wiggert A. Van Cappellen, Maarten W. Paul, Mehrnaz Ghazvini, Adriaan B. Houtsmuller, et al.expressing cells and whole testis was extracted using ReliaPrep™ RNA Cell Miniprep System (Promega, cat. No. Z6010) according to the manufacturer’s instructions. Purified RNA was used to make cDNA using SuperScript™

(6) Protocol for simultaneous profiling of transcription start sites and full-length transcripts from low-input samples using Smart-seq+5′STAR ProtocolsJune 19, 2026Diego Rodriguez-Terrones, Marlies E. Oomen, Maria-Elena Torres-Padillathe extracted RNA in nuclease-free water. NOTE: We have successfully used the Reliaprep kit (Promega #Z6011) for this step, however any RNA extraction method that results in pure and high integrity RNA can be

(7) Status Epilepticus Alters the Function of Brain-Derived Extracellular VesiclesJ Extracell VesiclesJune 17, 2026Samantha L. Reed, Yilin Wang, Yangping Li, Guoku Hu, Jingjing Yang, Bing Yao, Andrew EscaygBDEV‐treatment using the ReliaPrep RNA Cell Miniprep System per manufacturer's instructions (Promega Z6011). Samples were resuspended in 30 µL of RNase‐free water and stored at −80°C until further use. BDEV

(8) Pancreatic cancer induces B cell lineage plasticity via Pax5 inhibition to sustain immunosuppressionCell Death DiscovJune 2, 2026Ali Kassem, Nataly Naser Al Deen, Sun Yifeng, Chau Fang, Laura Mayer, Lingling Zhang, Paul Kunath, Thomas Wirth, Uwe Knippschild, Mohammad Rahbari, et al.16 h or 5 days. RNA was extracted by using the ReliaPrep RNA Cell Miniprep System (Promega, Cat. No. Z6012) according to instructions provided by the manufacturer. Residual genomic DNA was digested using DNase I

(9) L-DOPA enhances iRPE differentiation via Wnt signaling and improves cytotherapy for retinal degradationStem Cell Research & TherapyJune 2, 2026Yifeng Ke, Yingxiang Zhao, Qiang Feng, Min Xue, Mingxuan Zhang, Liangzhang Tan, Xinjun Rencells were harvested for total RNA extraction using the ReliaPrep™ RNA Cell Miniprep System (Promega Z6012), and cDNA was synthesized using the RT2 First Strand Kit (Qiagen 330404) following the manufacturer’s

(10) Food-Derived Elastin Peptides Improve Glucose Metabolism and Protect Renal Vasculature in Stroke-Prone Spontaneously Hypertensive Rats Despite Modest Dipeptidyl Peptidase 4 InhibitionNutrientsMay 30, 2026Kumiko Takemori, Yuki Nakamura, Kenji Sato, Eri Shiratsuchi, Takashi Kometani, Seiji Masudaextracted from both the glomerular and LRFs using the ReliaPrep™ RNA Cell Miniprep System (Promega, Z6010, Madison, WI, USA). Sample optical density was measured at 230, 260, and 280 nm using an e-Spect ES2

(11) Developmental programmes drive cellular plasticity, disease progression and therapy resistance in lung adenocarcinomaMolecular OncologyMay 27, 2026Kamila J Bienkowska, Stephany Gallardo, Nur S. Zainal, Leena Arora, Matthew Ellis, Maria‐Antoinette Lopez, Judith Austine, Sai Pittla, Serena J Chee, Aiman Alzetani, et al.by centrifugation (10s, 100xg). RNA was extracted using ReliaPrep RNA Cell Miniprep System (Promega. Z6011). cDNA was prepared using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1621)

(12) Inhibition of RNA polymerase II-activating CDK9 and CDK12/13, but not of cell cycle relevant CDKs, induces apoptosis by downregulating the short-lived Bcl-2 proteins Mcl1 and Bfl1/A1Cell Death DisMay 27, 2026Karina S. Krings, Judith Hatzfeld, Sandra Weller, Nadine Borkowski, Tanya R. Llewellyn, Laura Schmitt, Bo Scherer, Harri M. Itkonen, Christoph Peter, Björn Stork, et al.transcription quantitative PCR Total RNA extraction was performed using the ReliaPrep™ RNA Miniprep Kit (#Z6012, Promega). One microgram (1 µg) of RNA was reverse transcribed into cDNA using M-MLV Reverse Transcriptase

(13) Single-cell transcriptomics highlights macrophage-driven regulation of EndMT and repair in injured muscleCommunications BiologyMay 11, 2026Filipa Timóteo-Ferreira, Mauro Bergamaschi, Riccardo Gamberale, Cristiana Barone, Cristina D’Orlando, Anna Sofia Tascini, Veronica Bonalume, Raffaella Meneveri, Alessandro Fantin, Emanuele Azzoni, et al.extraction and cDNA synthesis Total RNA from mECs was isolated using ReliaPrep RNA Cell Miniprep System (Z6011; Promega, Milan, Italy), following manufacturer instructions. Total RNA was quantified by measuring

(14) Ultrasound modulates microglial activity and reduces neuroinflammation in a parameter-dependent mannerNPJ AcoustMay 6, 2026Sarina Grewal, Francesco Iacoponi, Lok Yin Nicholas Chan, Valeria Dosso, William Lim Kee Chang, Vanessa Drevenakova, Albert Ugwudike, Leonardo Ricotti, Paul M. Matthews, Andrea Cafarelli, et al.ReliaPrep™ RNA Cell Miniprep System according to the manufacturer’s instructions at 24, 48 and 72 h post-ultrasound (Promega, Cat. Z6011). Next, a NanoDrop spectrophotometer was used to assess the concentration and the purity of the RNA.

(15) A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal TransitionbioRxivApril 20, 2026Weimin Liu, Yusof Gusti, Fathima Athar, Naresh K Rajendran, Paul A. Cahill, Eileen M. Redmondalternate day. Real time qPCR. Total RNA was isolated using ReliaPrep TM RNA cell Miniprep System (Cat. # Z6011, Promega) following manufacturer’s instructions. DNase-treated RNA was either used in one-step qRT-PCR

(16) Transient remodeling of gut metabolism supports juvenile growth and adult fitness in DrosophilaNat CommunApril 13, 2026Clara Lefranc, Arnaud Fichant, Gilles Storellifurther processing. Total RNA was extracted using the Reliaprep RNA Tissue Miniprep System (Promega, Cat#Z6012). 500 µL of lysis buffer was added to the microtubes and tissues were homogenized using a Precellys

(17) Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary FibroblastsbioRxivApril 5, 2026Nicolas P. Holcomb, Ronel Z. Samuel, Alena Klochkova, Joanna Wong, Sara Kass-Gergi, Meryl Mendoza, Michael M. Maiden, Evelyn A. Martinez, Diana M. Abraham, Madeline Singh, et al.Isolation and RT-qPCR: RNA was isolated using the either the ReliaPrep™ RNA Cell Miniprep kit (Promega, Z6011) or Direct-zol RNA Miniprep Plus (ZymoResearch; R2072) and concentration/quality was assessed via nanodrop.

(18) Chromatin dynamics of the Klf4 locus in mouse pluripotent cellsScientific ReportsMarch 27, 2026Jente van Staalduinen, Hélène Kabbech, Selçuk Yavuz, Ridvan Cetin, Agnese Loda, Wiggert van Cappellen, Adriaan Houtsmuller, Kerstin Wendt, Ihor Smal, Frank Grosveldnaïve and differentiation conditions with the ReliaPrep RNA Cell Miniprep system (Promega, Catalog no: Z6012) according to manufacturer’s instructions. After RNA isolation, RNA was treated with 1 μL TURBO DNase

(19) IGFBP3 repression driven by inflammation links air pollution to placental and developmental defectsEMBO Molecular MedicineMarch 24, 2026Sunil Singh, Isha Goel, Anubhuti Rana, Anamta Gul, Javed A Quadri, Asit Ranjan Mridha, Lakshay Malhotra, Neha Kashyap, Baburajan Radha, Arnab Nayek, et al.Cell Assay Kit,Himedia Laboratories Private Ltd.,CCK032 Total RNA isolation kit,Promega Corporation,Z6011 Nanodrop,"Thermo Fisher Scientific, Inc", Verso cDNA synthesis Kit,"Thermo Fisher Scientific, Inc.",AB1453A DyNAmo

(20) Long-term exposure to nanoparticles alters senescence-associated markers and immune responses in human monocyte-derived macrophagesNanoscaleMarch 18, 2026Henry Lee, Moritz Häffner, Sandor Balog, Patricia Taladriz-Blanco, Aurélien Mroczek, Simona Staudinger, Alke Petri-Fink, Barbara Rothen-RutishauserMadison, WI, USA), and total RNA was extracted using the ReliaPrep™ RNA Cell Miniprep System (Promega, Z6012, Madison, WI, USA). RNA concentration and quality were verified using a NanoDrop™ 2000 spectrophotometer

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