ReliaPrep RNA Cell Miniprep System from Promega

Product Specs
ItemReliaPrep RNA Cell Miniprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ6011
Quantity50 preps
SourceCell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeCultured cells or tissue

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Inhibition of RNA polymerase II-activating CDK9 and CDK12/13, but not of cell cycle relevant CDKs, induces apoptosis by downregulating the short-lived Bcl-2 proteins Mcl1 and Bfl1/A1Cell Death DisMay 27, 2026Karina S. Krings, Judith Hatzfeld, Sandra Weller, Nadine Borkowski, Tanya R. Llewellyn, Laura Schmitt, Bo Scherer, Harri M. Itkonen, Christoph Peter, Björn Stork, et al.transcription quantitative PCR Total RNA extraction was performed using the ReliaPrep™ RNA Miniprep Kit (#Z6012, Promega). One microgram (1 µg) of RNA was reverse transcribed into cDNA using M-MLV Reverse Transcriptase

(2) Ultrasound modulates microglial activity and reduces neuroinflammation in a parameter-dependent mannerNPJ AcoustMay 6, 2026Sarina Grewal, Francesco Iacoponi, Lok Yin Nicholas Chan, Valeria Dosso, William Lim Kee Chang, Vanessa Drevenakova, Albert Ugwudike, Leonardo Ricotti, Paul M. Matthews, Andrea Cafarelli, et al.ReliaPrep™ RNA Cell Miniprep System according to the manufacturer’s instructions at 24, 48 and 72 h post-ultrasound (Promega, Cat. Z6011). Next, a NanoDrop spectrophotometer was used to assess the concentration and the purity of the RNA.

(3) A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal TransitionbioRxivApril 20, 2026Weimin Liu, Yusof Gusti, Fathima Athar, Naresh K Rajendran, Paul A. Cahill, Eileen M. Redmondalternate day. Real time qPCR. Total RNA was isolated using ReliaPrep TM RNA cell Miniprep System (Cat. # Z6011, Promega) following manufacturer’s instructions. DNase-treated RNA was either used in one-step qRT-PCR

(4) Transient remodeling of gut metabolism supports juvenile growth and adult fitness in DrosophilaNat CommunApril 13, 2026Clara Lefranc, Arnaud Fichant, Gilles Storellifurther processing. Total RNA was extracted using the Reliaprep RNA Tissue Miniprep System (Promega, Cat#Z6012). 500 µL of lysis buffer was added to the microtubes and tissues were homogenized using a Precellys

(5) Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary FibroblastsbioRxivApril 5, 2026Nicolas P. Holcomb, Ronel Z. Samuel, Alena Klochkova, Joanna Wong, Sara Kass-Gergi, Meryl Mendoza, Michael M. Maiden, Evelyn A. Martinez, Diana M. Abraham, Madeline Singh, et al.Isolation and RT-qPCR: RNA was isolated using the either the ReliaPrep™ RNA Cell Miniprep kit (Promega, Z6011) or Direct-zol RNA Miniprep Plus (ZymoResearch; R2072) and concentration/quality was assessed via nanodrop.

(6) Chromatin dynamics of the Klf4 locus in mouse pluripotent cellsScientific ReportsMarch 27, 2026Jente van Staalduinen, Hélène Kabbech, Selçuk Yavuz, Ridvan Cetin, Agnese Loda, Wiggert van Cappellen, Adriaan Houtsmuller, Kerstin Wendt, Ihor Smal, Frank Grosveldnaïve and differentiation conditions with the ReliaPrep RNA Cell Miniprep system (Promega, Catalog no: Z6012) according to manufacturer’s instructions. After RNA isolation, RNA was treated with 1 μL TURBO DNase

(7) IGFBP3 repression driven by inflammation links air pollution to placental and developmental defectsEMBO Molecular MedicineMarch 24, 2026Sunil Singh, Isha Goel, Anubhuti Rana, Anamta Gul, Javed A Quadri, Asit Ranjan Mridha, Lakshay Malhotra, Neha Kashyap, Baburajan Radha, Arnab Nayek, et al.Cell Assay Kit,Himedia Laboratories Private Ltd.,CCK032 Total RNA isolation kit,Promega Corporation,Z6011 Nanodrop,"Thermo Fisher Scientific, Inc", Verso cDNA synthesis Kit,"Thermo Fisher Scientific, Inc.",AB1453A DyNAmo

(8) Long-term exposure to nanoparticles alters senescence-associated markers and immune responses in human monocyte-derived macrophagesNanoscaleMarch 18, 2026Henry Lee, Moritz Häffner, Sandor Balog, Patricia Taladriz-Blanco, Aurélien Mroczek, Simona Staudinger, Alke Petri-Fink, Barbara Rothen-RutishauserMadison, WI, USA), and total RNA was extracted using the ReliaPrep™ RNA Cell Miniprep System (Promega, Z6012, Madison, WI, USA). RNA concentration and quality were verified using a NanoDrop™ 2000 spectrophotometer

(9) ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage-like foam cells in atherosclerosisCardiovascular ResearchMarch 16, 2026Bryce R Evans, Julia Schulz, Vasiliki Triantafyllidou, Anais Yerly, Manovriti Thakur, Nico Angliker, Mark Siegrist, Yvonne Jansen, Yi Yan, Sanne L Maas, et al.α-NETA or 100 nM C9, which was maintained overnight. RNA was isolated using the Promega RNA Miniprep (Z6011) Systems and quantified using a NanoDrop™ 2000/2000c spectrophotometer (Thermo Fisher, Waltham, MA,

(10) Insights from pooled CRISPRi single-cell screens in K562 cells reveal gene functions, regulatory networks, and highlight opportunities and limitationsBMC GenomicsMarch 13, 2026Hong Zhang, Peifen Zhang, Eric Bindels, Eskeatnaf Mulugetaextraction. RT-qPCR Total RNA was isolated using ReliaPrep™ RNA Cell Miniprep System (Promega, catalog no. Z6012) and converted to cDNA using SuperScipt IV Reverse Transcriptase kit (Invitrogen, catalog no. 18090200)

(11) RNA binding protein Sam68 promotes germinal center reaction and IgG response through regulation of miR29Cell Mol Life SciMarch 7, 2026Moumita Datta, Valerio Renna, Manish Kumar, Palash Chandra Maity, Hassan Jumaadescribed previously. RNA was isolated from the sorted cells using ReliaPrep Cell RNA miniprep kit (#Z6011, Promega). Fifty nanogram of total RNA was reverse transcribed using high-capacity RNA to cDNA kit (#4387406,

(12) Nppa and Nppb Deficiency Drives Ventricular Hypertrophy and Subendocardial Gene Deregulation in the Mouse HeartInt J Mol SciMarch 6, 2026Alexandra E. Giovou, Otto J. Mulleners, Marie Günthel, Joyce C. K. Man, Bjarke Jensen, Monika M. Gladka, Vincent M. ChristoffelsqPCR Total RNA was isolated from mouse left ventricles using ReliaPrep™ RNA Miniprep Systems (Promega, #Z6012, Madison, WI, USA) according to the manufacturer’s protocol. 1 μg of total RNA was reverse transcribed

(13) Postbiotic Metabolites from a 31-Strain Lactobacillus/Bifidobacterium Co-Culture Attenuate DSS Colitis with Barrier- and Circadian-Linked Transcriptomic SignaturesBiology (Basel)March 5, 2026Shuhei Ueda, Takumi Iwasawa, Kaho Ohki, Satoshi Takeda, Ryohma Tsuchiya, Shunsuke Sakuraba, Kazunori Kato, Tomoaki ItoQuantitative PCR (qPCR) Total RNA was extracted using ReliaPrep™ RNA Miniprep Systems (Promega, Madison, WI, USA; Z6010 according to the manufacturer’s protocol). RNA was quantified using DeNovix DS-11 FX. Reverse transcription

(14) Alignment of in vitro and in vivo pulmonary inflammation models using crystalline quartz silicaParticle and Fibre ToxicologyFebruary 19, 2026Isidora Loncarevic, Seyran Mutlu, Martina Dzepic, Sandeep Keshavan, Sandor Balog, Alke Petri-Fink, Fabian Blank, Barbara Rothen-Rutishauserwas performed directly on the insert, using 100 µL of BL + TG buffer (Promega Madison, WI, USA, Ref. Z6011), and total RNA was extracted using ReliaPrep™ RNA Cell Miniprep System (Promega, Madison, WI, USA,

(15) Electrolyzed Hydrogen Water Improves Chemosensitivity to Anticancer Drugs by Potently Suppressing AutophagyJournal of Cellular and Molecular MedicineFebruary 17, 2026Satoshi Yano, Liangjing Xie, Jinjuan Li, Yuka Sugaya, Yuki Miyauchi, Shigeru Kabayama, Taichi Harah. Total RNA was isolated using the ReliaPrep RNA Cell Miniprep Systems (Promega, Madison, WI, USA; Z6012), following the manufacturer's instructions. Reverse transcription of RNA to cDNA was performed using

(16) Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylationNucleic Acids ResFebruary 12, 2026Marika Oksanen, Francesca Mastropasqua, Krystyna Mazan-Mamczarz, Jennifer L Martindale, Xuan Ye, Abishek Arora, Nirad Banskota, Myriam Gorospe, Kristiina Tammimiesextraction columns, and the final extraction was carried out with ReliaPrep RNA Cell Miniprep kit (Promega Z6012). The RNA was reverse transcribed using iScript complementary DNA (cDNA) Synthesis Kit (BioRad) and

(17) Miquelianin and spiraeoside from Filipendula ulmaria mitigate α-synuclein accumulation in C.elegans and reduce the expression of neuroinflammatory cytokines in human microgliaFrontiers in PharmacologyFebruary 11, 2026Martina Redl, Sabrina Weisenburger, Andreas Wasilewicz, Ulrike Grienke, Martin D. Lehner, Dirk Bredenbröker, Judith M. Rollingerharvested cells after LPS-stimulation and treatment using the ReliaPrep RNA Cell MiniPrep System (Promega, #Z6010) following the manufacturer’s protocol. Briefly, cells were lysed in guanidine thiocyanate and 1-thioglycerol

(18) BMP9 Modulates IL-33 Signaling to Mitigate EndMT in Pulmonary Arterial HypertensionHypertensionFebruary 1, 2026Clarissa Becher, Esmee J. Groeneveld, Rozenn Quarck, Beau Neep, Xiaoke Pan, Robert Szulcek, Ly Tu, Christophe Guignabert, Harm Jan Bogaard, Paul B. Yu, et al.sulfoxide, followed by BMP9 (1 ng/mL, 3 hours). RNA was extracted using the RNA Miniprep System (Promega, Z6012) after PBS washes. cDNA synthesis was performed with 500-ng RNA using the RevertAid First Strand cDNA Synthesis Kit

(19) BMP9 Modulates IL-33 Signaling to Mitigate EndMT in Pulmonary Arterial HypertensionHypertensionJanuary 22, 2026Clarissa Becher, Esmee J. Groeneveld, Rozenn Quarck, Beau Neep, Xiaoke Pan, Robert Szulcek, Ly Tu, Christophe Guignabert, Harm Jan Bogaard, Paul B. Yu, et al.sulfoxide, followed by BMP9 (1 ng/mL, 3 hours). RNA was extracted using the RNA Miniprep System (Promega, Z6012) after PBS washes. cDNA synthesis was performed with 500-ng RNA using the RevertAid First Strand cDNA Synthesis Kit

(20) Acute activation of Gq-signaling in islet macrophages inhibits β-cell insulin secretion through AMPK-sphingolipid axisbioRxivJanuary 16, 2026Simran Singh, Ashish Kumar, Sudipta Paul, Mriganka Sarkar, Santhosh Duraisamy, Harender Yadav, Seema Kuldeep, Soumita Bhaumik, Kunj Kumar Prajapati, Saahiba Thaleshwari, et al.Insulin Chemiluminescence ELISA (Ex vivo),ALPCO,80-INSMR-CH01 ReliaPrep™ RNA Miniprep Systems,Promega,Z6010 PrimeScript 1st strand cDNA Synthesis Kit,Takara,6110A HTRF IP-One Gq Detection Kit,Revvity,62IPAPEB BCA protein assay kit

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