Amylose Resin High Flow from New England Biolabs

Product Specs
ItemAmylose Resin High Flow
CompanyNew England Biolabs
Price $353.00 Inquire
Catalog NumberE8022S
CapacityMBP2 -paramyosin DeltaSal fusion protein 7 mg/ml
Quantity15 ml
Matrixcross-linked affinity matrix
ApplicationsProteomics & Glycomics
Product TypeResins
ModeAffinity Purification
TypeResin
Temperature4 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The nuclear envelope protein TMEM209 is an integral component of the nuclear pore complex and interacts with Nup210Journal of Cell ScienceJune 15, 2026David Kohlhause, Christiane Spillner, Violeta Alcalde Zapata, Christof Lenz, Henning Urlaub, Tobias Kohl, Stephan E. Lehnart, Larry Gerace, Ralph H. Kehlenbachprotease inhibitors as above). For protein purification, lysates were incubated at 4°C for 2 h with 2 ml Amylose Resin High Flow (New England Biolabs) equilibrated in MBP standard buffer (50 mM Tris-HCl pH 7.4, 200 mM NaCl, 5 mM

(2) Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptaseNat Struct Mol BiolJune 10, 2026Nolan Neville, Nicole V. Johnson, Edwin E. Escobar, Chang-Hwa Chiang, Albana Nreca, Sean R. Johnson, Nan Dai, Andy Hanneman, Ivan R. Corrêa, Jason S. McLellan, et al.at 20,000g for 20 min at 4 °C. Supernatants were mixed with 6 ml of 50% slurry amylose resin (NEB, E8022L) that had been pre-washed three times in pulldown lysis buffer. The lysates were rocked in 50-ml conical

(3) Phosphorylation of RBM39 by CDK13 stabilizes RAD50 mRNA to drive cisplatin resistance in endometrial cancerJournal of Biological ChemistryApril 15, 2026Chenxiao Yang, Xiaoyan Zheng, Hao Sun, Juntong Du, Youjun Luo, Chang Wang, Yunlong Wu, Liying Qu, Fang Yuan, Zhan Yangcyclin K were coexpressed in E. coli BL21 (DE3) and purified using amylose resin (New England Biolabs, E8022S) according to the manufacturer's instructions. HA-tagged WT RBM39 (RBM39-WT) and the phosphorylation-defective

(4) Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotus japonicusPlant CommunicationsApril 13, 2026Yanan Wang, Hanbin Bao, Yutao Lei, Zhongmin Zou, Lifa Yuan, Haoxing Li, Hui Zhu, Dawei Xin, Christian Staehelin, Yangrong Caofor 10 min at 4°C. The supernatant was incubated with 20 μL amylose resin beads (New England Biolabs, E8022S) at 4°C for 3 h. Beads were washed at least five times with column buffer. Immunoblotting was performed

(5) A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memoryNature CommunicationsJanuary 9, 2026Chenming Zeng, Giulia Furlan, Miguel Vasconcelos Almeida, Juan C. Rueda-Silva, Jonathan Price, Helena Santos-Rosa, Jingxiu Xu, Yan Kuang, Enric Cata Socias, Jonas Mars, et al.supernatant was subjected to affinity purification using GST beads (Cytiva, #17527901) or MBP beads (NEB, #E8022L). Then, the beads were washed three times with washing buffer (50 mM Tris-HCl PH 7.5 and 150 mM NaCl).

(6) CpoS-Inc interactions facilitate host cell modulation during Chlamydia trachomatis infectionInfection and ImmunityDecember 16, 2025Xavier Tijerina, C.A. Jabeena, Robert Faris, Zhen Xu, Parker Smith, Nicholas J. Schnicker, Mary M. Weberhigh-speed centrifugation at 12,000 × g for 1 h and applied to an Amylose resin high-flow column (NEB Cat#E8022S). Unbound proteins were removed by washing with sonication buffer, and bound proteins were eluted with

(7) Integrative chromosome-level genomics and metabolomics uncover regulatory networks linking monoterpenoid biosynthesis and glandular trichome formation in Mosla chinensisHorticulture ResearchOctober 1, 2025Muyao Yu, Chenyi Li, Xiaoqing Wang, Dan Jiang, Xueqing Fu, Chao Chen, Guangxi Ren, Xuewei Li, Yaojie Zhang, Qi Liu, et al.(IPTG), 28°C, 12 h with 220 rpm orbital shaking). Purification of the target proteins was achieved using Amylose Resin High Flow (NEB, Ipswich, MA, USA). The purified protein was incubated for 3 h in a reaction mixture containing

(8) The Jasmonate‐Responsive SmMPK3–SmWRKY33 Module Positively Regulates Tanshinone Biosynthesis in Salvia miltiorrhizaPlant Biotechnology JournalSeptember 8, 2025Renjun Qu, Shiwei Wang, Xinxin Wang, Jiaming Peng, Juan Guo, Guanghong Cui, Tong Chen, Meilan Chen, Shuangshuang Zhao, Ye ShenEscherichia coli BL21(DE3). Recombinant proteins were purified using Amylose Resin (NEB, New England; cat# E8022S). Biotin‐labelled oligonucleotide probes (approximately 45 or 30 bp in length) containing specific W‐box

(9) Cryo-EM structures of a pentameric ligand-gated ion channel in liposomesElifeJuly 16, 2025Vikram Dalal, Brandon K Tan, Hanrui Xu, Wayland W L Cheng1% DDM (Anatrace D310S). The extracted protein was purified with amylose resin (New England Biolabs E8022L) and eluted with 40 mM maltose in buffer A (10 MM Tris pH 7.5, 100 mM NaCl, 0.02% DDM). The maltose

(10) Cryo-EM structures of a pentameric ligand-gated ion channel in liposomesElifeJuly 16, 2025Vikram Dalal, Brandon K Tan, Hanrui Xu, Wayland WL Cheng1% DDM (Anatrace D310S). The extracted protein was purified with amylose resin (New England Biolabs E8022L) and eluted with 40 mM maltose in buffer A (10 MM Tris pH 7.5, 100 mM NaCl, 0.02% DDM). The maltose

(11) Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regionsScientific reportsJuly 1, 2025Bakosova A, Cipak L, Mayerova N, Krol K, Benko Z, Pitelova A, Kolesar P, Piatrova D, Smondrkova M, Maresova A, et al.of 60–600 mM KCl in buffer K. The fractions containing Dbl2 were pooled and incubated with 2 ml of Amylose Resin High Flow (NEB) for 1 h at 4 °C. The resin was washed with 50 ml of buffer K containing 300 mM KCl, and the proteins

(12) The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagyElifeJune 24, 2025Yutaro Hama, Yuko Fujioka, Hayashi Yamamoto, Noboru Mizushima, Nobuo N NodaChemical Corporation,163-20145, "Chemical compound, drug",Amylose Resin High Flow,New England Biolabs,E8022L, "Chemical compound, drug",Bio-Scale Mini Bio-Gel P-6 desalting column,Bio-Rad Laboratories,7325304, "Chemical

(13) The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagyElifeJune 24, 2025Yutaro Hama, Yuko Fujioka, Hayashi Yamamoto, Noboru Mizushima, Nobuo N NodaChemical Corporation,163-20145, "Chemical compound, drug",Amylose Resin High Flow,New England Biolabs,E8022L, "Chemical compound, drug",Bio-Scale Mini Bio-Gel P-6 desalting column,Bio-Rad Laboratories,7325304, "Chemical

(14) Molecular mechanism of PP2A/B55α phosphatase inhibition by IER5Cell Chemical BiologyApril 17, 2025Ruili Cao, Daniel T.D. Jones, Li Pan, Annie Yang, Shumei Wang, Sathish K.R. Padi, Shaun Rawson, Jon C. Aster, Stephen C. Blacklowresin,NEUROMICS,Cat # 2-5030-025 Anti-Flag resin,Produced in-house,N/A Amylose Resin High Flow,NEB,Cat# E8022S Compound E,EMD Millipore,Cat # 565790 Critical commercial assays,Critical commercial assays,Critical

(15) Control of 3′ splice site selection by the yeast splicing factor Fyv6ElifeDecember 17, 2024Katherine A Senn, Karli A Lipinski, Natalie J Zeps, Amory F Griffin, Max E Wilkinson, Aaron A Hoskinsdrug",Ni-NTA agarose,QIAGEN,Cat #: 30210, "Chemical compound, drug",Amylose resin,New England Biolabs,Cat #: E8022S, "Chemical compound, drug",Fluorescein-5-thiosemicarbazide,Sigma-Aldrich,Cat #: 46985-100MG-F, "Software,

(16) Control of 3′ splice site selection by the yeast splicing factor Fyv6eLifeDecember 17, 2024Katherine A Senn, Karli A Lipinski, Natalie J Zeps, Amory F Griffin, Max E Wilkinson, Aaron A Hoskinsdrug",Ni-NTA agarose,QIAGEN,Cat #: 30210, "Chemical compound, drug",Amylose resin,New England Biolabs,Cat #: E8022S, "Chemical compound, drug",Fluorescein-5-thiosemicarbazide,Sigma-Aldrich,Cat #: 46985-100MG-F, "Software,

(17) Selective cargo and membrane recognition by SNX17 regulates its interaction with RetrieverEMBO ReportsDecember 9, 2024Aurora Martín-González, Iván Méndez-Guzmán, Maialen Zabala-Zearreta, Andrea Quintanilla, Arturo García-López, Eva Martínez-Lombardía, David Albesa-Jové, Juan Carlos Acosta, María Lucas75105 Streptactin-XT-4Flow Resin,IBA Lifesciences,Cat# 2-5030-010 Amylose Resin High Flow,New England Biolabs,Cat# E8022S HitrapQ,Cytiva,Cat# 17115401 Superdex75 10/300 GL,Cytiva,Cat# 17517401 Superdex200 Increase 10/300

(18) Negative regulation of APC/C activation by MAPK-mediated attenuation of Cdc20Slp1 under stressElifeOctober 16, 2024Li Sun, Xuejin Chen, Chunlin Song, Wenjing Shi, Libo Liu, Shuang Bai, Xi Wang, Jiali Chen, Chengyu Jiang, Shuang-min Wang, et al.(DE3) cells. The recombinant proteins were purified by glutathione Sepharose 4B (GE Healthcare) or Amylose Resin High Flow (New England Biolabs), respectively, according to the manufacturers’ instructions. In vitro pulldown

(19) Transcriptome-informed identification and characterization of Planococcus citri cis- and trans-isoprenyl diphosphate synthase genesiScienceApril 19, 2024Mojca Juteršek, Iryna M. Gerasymenko, Marko Petek, Elisabeth Haumann, Sandra Vacas, Kalyani Kallam, Silvia Gianoglio, Vicente Navarro-Llopis, Michael Heethoff, Ismael Navarro Fuertes, et al.proteins" ,, TRIzol Reagent,ThermoFisher Scientific,Cat# 15596026 Amylose resin,New England Biolabs,E8022 ZB-5MS fused silica capillary column,Phenomenex,N/A CycloSil-B column,Agilent,112-6632 Zorbax 300Extend-C18,Agilent,763973-902 Calf

(20) Unveiling the regulatory mechanisms of nodules development and quality formation in Panax notoginseng using multi-omics and MALDI-MSIJournal of Advanced ResearchApril 7, 2024Muyao Yu, Chunxia Ma, Badalahu Tai, Xueqing Fu, Qi Liu, Guanhua Zhang, Xiuteng Zhou, Liyuan Du, Yan Jin, Yang Han, et al.binding protein (MBP) was performed at 25 °C for 12 h, 30 °C for 8 h, and 25 °C for 12 h, respectively. Amylose Resin High Flow (NEB, Ipseich, MA, USA) was subsequently used to obtain purified protein. EMSA was performed according

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