Single-Stranded DNA Binding Protein from Promega

Product Specs
ItemSingle-Stranded DNA Binding Protein
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM3011
Quantity100microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Genome-wide single-cell perturbation screens with VIPerturb-seqbioRxivFebruary 16, 2026Alexandra Bradu, John D. Blair, Isabella N. Grabski, Isabella Mascio, Junsuk Lee, Cecilia McCormick, Rahul Satijaprepared as described above was incubated with 8μg of single-stranded DNA binding protein (Promega M3011) per 1μg of antibody in a solution of 1x NEB buffer 4 (NEB B7004S) for 30 minutes at 37 °C. After completing

(2) Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprintingbioRxivSeptember 24, 2025Isabella N. Grabski, Junsuk Lee, John Blair, Carol Dalgarno, Isabella Mascio, Alexandra Bradu, David A. Knowles, Rahul Satijahashing step, the primary antibody pool was incubated with single-stranded DNA binding protein (Promega: M3011) to help reduce off-target effects76,77. Briefly, 8μg of SSB was added per 1μg of antibody in a solution

(3) Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulatorsbioRxivSeptember 11, 2025John D. Blair, Alexandra Bradu, Carol Dalgarno, Isabella N. Grabski, Rahul Satijahashing step, the primary antibody pool was incubated with single-stranded DNA binding protein (Promega: M3011) to help reduce off-target effects14,23. Briefly, 8 μg of SSB was added per 1 μg of antibody in a solution

(4) Integration of phospho-signaling and transcriptomics in single cells reveals distinct Th17 cell fatesCell reportsAugust 26, 2025Fortmann SD, Chadha AS, Frey BF, Elahi A, Hanumanthu VS, Liu S, Goldsborough A, Ferrell PB Jr, Grant MB, Weaver CT, et al.Ab-oligonucleotide conjugates were then incubated with E. coli ssDNA binding protein (EcoSSB; Promega; M3011) to prevent off-target oligonucleotide interactions, as recently reported.14 To do this, we first calculated

(5) Endothelial TDP-43 depletion disrupts core blood–brain barrier pathways in neurodegenerationNature NeuroscienceMarch 14, 2025Omar M. F. Omar, Amy L. Kimble, Ashok Cheemala, Jordan D. Tyburski, Swati Pandey, Qian Wu, Bo Reese, Evan R. Jellison, Bing Hao, Yunfeng Li, et al.followed by a 10-min incubation. Before using the antibody mix, we incubated it with EcoSSB (Promega M3011) in 50 μl 1× NEBuffer 4 for 30 min at 37 °C as suggested previously20. The nuclei were then stained

(6) Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cellsNat CommunFebruary 4, 2025John D. Blair, Austin Hartman, Fides Zenk, Philipp Wahle, Giovanna Brancati, Carol Dalgarno, Barbara Treutlein, Rahul Satijathat received a primary antibody bound by EcoRI single-stranded DNA binding protein (SSB) (Promega: M3011), the SSB binding reaction happened during the blocking step. Briefly, 1 μg of antibody was incubated

(7) Targeted inCITE-Seq Analysis Identifies the Loss of Nuclear TDP-43 in Endothelium as a Mediator of Blood Brain Barrier Signaling Pathway Dysfunction in NeurodegenerationbioRxivJanuary 19, 2024Omar M.F. Omar, Amy L. Kimble, Ashok Cheemala, Jordan D. Tyburski, Swati Pandey, Qian Wu, Bo Reese, Evan R. Jellison, Yunfeng Li, Bing Hao, et al.stock), and InCITE seq antibody mix. Before using the antibody mix, we incubated it with EcoSSB (Promega M3011) in 50Oμl of 1X NEBuffer 4 for 30Omin at 37O°C as suggested previously 18. Afterwards, 1 μl of TotalSeqB

(8) Preparation and processing of dried blood spots for microRNA sequencingBiology Methods and ProtocolsSeptember 20, 2023Alice Morgunova, Pascal Ibrahim, Gary Gang Chen, Saché M Coury, Gustavo Turecki, Michael J Meaney, Anthony Gifuni, Ian H Gotlib, Corina Nagy, Tiffany C Ho, et al.equivalent) Reagents for adapter depletion Escherichia coli single-stranded DNA binding protein (SSB) (Promega M3011)5′ deadenylase (NEB M0331)RecJf (NEB M0264) Reagents for 5′-ligation 10 mM ATP (NEB P0756)T4 RNA ligase

(9) NEAT-seq: simultaneous profiling of intra-nuclear proteins, chromatin accessibility and gene expression in single cellsNature methodsMay 1, 2022Chen AF, Parks B, Kathiria AS, Ober-Reynolds B, Goronzy JJ, Greenleaf WJ.Fig. 6a). Binding of single stranded DNA binding protein to oligo-antibodies To bind EcoSSB (Promega M3011) to the antibody-oligos, we incubated the antibody and EcoSSB in 50ul of 1X NEBuffer 4 for 30 min at

(10) Influence of the C-Terminal Tail of RecA Proteins from Alkaline pH-Resistant Bacterium Deinococcus FicusACS OmegaAugust 11, 2020Hsiu-Fang Fan, Shu Su, Ying-An Kuo, Cyuan-Ji Chenmin with or without an additional 5 min of incubation in the presence of 50 nM E. coli SSB (Promega, M3011). The reaction was initiated after the addition of homologous 500-nt Cy3-dsDNA or 500-nt ssDNA and quenched

(11) Comparison of Reproducibility, Accuracy, Sensitivity, and Specificity of miRNA Quantification PlatformsCell ReportsDecember 17, 2019Paula M. Godoy, Andrea J. Barczak, Peter DeHoff, Srimeenakshi Srinivasan, Alton Etheridge, David Galas, Saumya Das, David J. Erle, Louise C. LaurentRNA Ligase 2, Deletion Mutant",NEB,Cat#M0242L E. Coli Single Stranded Binding Protein,Promega,Cat#M3011 5' Deadenylase,NEB,Cat#M0331 RecJF,NEB,Cat#M0264 SuperScript II,Invitrogen,Cat#18064-014 ,, Deposited

(12) Library Preparation for small RNA sequencing using 4N adapters: In house 4N Protocol CProtocol ExchangeMay 15, 2018Roger P. Alexander et al.**Reagents for adapter depletion** - _E. coli_ single-stranded DNA binding protein \(SSB) \(Promega M3011) - 5’ deadenylase \(NEB M0331) - RecJf \(NEB M0264) **Reagents for 5’ Ligation** - 10mM ATP \(NEB P0756)

(13) Modified TruSeq Small RNA Library Prep using Randomized 4N Adapters: In house 4N Protocol DProtocol ExchangeMay 15, 2018Roger P. Alexander et al.**Reagents for adapter depletion** - _E. coli_ single-stranded DNA binding protein \(SSB) \(Promega M3011) - 5’ deadenylase \ (NEB M0331) - RecJf \(NEB M0264) **Reagents for 5’ Ligation** - 10mM ATP and T4

(14) Library Preparation for small RNA sequencing using 4N adapters: In house 4N Protocol BProtocol ExchangeMay 15, 2018Roger P. Alexander et al.**Reagents for adapter depletion** - _E. coli_ single-stranded DNA binding protein \(SSB) \(Promega M3011) - 5’ deadenylase \(NEB M0331) - RecJf \(NEB M0264) **Reagents for 5’ Ligation** - 10mM ATP \(NEB P0756)

(15) Library Preparation for small RNA sequencing using 4N adapters: In house 4N Protocol AProtocol ExchangeMay 15, 2018Roger P. Alexander et al.equivalent) **Reagents for adapter depletion** - _E. coli_ single-stranded DNA binding protein \(SSB) \(Promega M3011) - 5’ deadenylase \(NEB M0331) - RecJf \(NEB M0264) **Reagents for 5’ Ligation** - 10mM ATP \ (NEB P0756)

(16) Monitoring the Transcriptional Activity of Human Endogenous Retroviral HERV-W Family Using PNA Strand Invasion into Double-Stranded DNAMolecular BiotechnologyFebruary 1, 2018Grzegorz Machnik et al.and then centrifuged briefly and cooled on ice. Single-Stranded DNA-Binding Protein, SSB (Cat. No. M3011) was purchased from Promega GmbH (Mannheim, Germany). The linear character of PNA-mediated strand invasion

(17) Force and ATP hydrolysis dependent regulation of RecA nucleoprotein filament by single-stranded DNA binding proteinNucleic Acids ResearchJanuary 1, 2013Hongxia Fu, Shimin Le, Hu Chen, K. Muniyappa, Jie Yantemporal resolution of 2 ms. E. coli SSB was purchased from Promega Corporation (The catalog number is M3011). E. coli RecA protein was purchased from New England BioLabs, USA (The catalog number is M0249L) or

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