Lentivirus Packaging Service Lentivirus is a versatile and highly utilized viral vector used in many areas of research and development including cell and gene therapy, immunology, and oncology. Our team is able to package your plasmid DNA construct into lentivirus particles with a variety of production options.
Below is a list of the research grade lentiviral vector packaging services we currently provide. If you're unsure which packaging service is right for your research, our team is more than happy to discuss your project.
Lentivirus Packaging Service Requirements To get started on your lentivirus packaging production, or start a formal quote, the following information is needed:
Lentivirus Packaging Generation: Our facility produces second generation lentivirus packaging and third generation lentivirus packaging productions. Learn more about lentivirus packaging generations. Researchers favor 2nd or 3rd generation systems for better gene delivery and safety. We offer both for lentivirus packaging.
- 1st Generation: Uses a three-plasmid system: transfer vector (gene of interest), packaging plasmid (gag, pol, rev), and envelope plasmid (e.g., VSV-G).
- 2nd Generation: Enhanced safety with mutations in the packaging plasmid to minimize replication-competent lentiviruses (RCLs), reducing biosafety risks.
- 3rd Generation: Further improved safety by separating gag/pol and rev into different plasmids, lowering the risk of recombination and RCL formation.
Production Scale: Our viral vector team can produce lentivirus packaging productions in 250 µl; 500 µl; 1 ml batches. For higher volumes, please indicate so when requesting a quote.
Titer and Production Purity: We measure viral titer RT-qPCR, utilizing primers that target the long-inverted terminal repeat (LTR) within the viral genome. Depending on the plasmid DNA construct, viral titers can range 10
8 TU/ml – 10
9 TU/ml.
Plasmid DNA Requirements: To begin production, we require only 1 µg of your plasmid DNA. We cover all plasmid prep expenses for your lentivirus production. Our plasmid preparations are always performed in house and result in endotoxin free and animal free plasmid DNA.
Transfection Protocol: Viral payload, cell line, cell type, and lentivirus generation can greatly impact transfection efficiency. Because of this, we produce every production to our client's lentivirus transfection protocol specifications. If you do not have a lentivirus transfection protocol, we can determine an appropriate one based on your research needs.
Quality Control Assay Options: All lentivirus productions have their titer levels measured with a RT-qPCR assay. In addition, we can perform the following quality control tests upon request:
- Fluorescence microscopy
- Flow cytometry
- Western blot