RsaI from New England Biolabs

Product Specs
ItemRsaI
CompanyNew England Biolabs
Price $67.00 Inquire
Catalog NumberR0167S
Concentration10 u/µL
Quantity1000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GT/AC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) RNF4 prevents genomic instability caused by chronic DNA under-replicationDNA Repair (Amst)March 1, 2024Marissa K. Oram, Ryan M. Baxley, Emily M. Simon, Kevin Lin, Ya-Chu Chang, Liangjun Wang, Chad L. Myers, Anja-Katrin Bielinskygel electrophoresis. Subsequently, 30 to 40 μg of genomic DNA was digested with HinfI (NEB R0155) and RsaI (NEB R0167). For each sample, 8 to 12 μg of digested genomic DNA was resolved overnight on a 0.7% agarose 1x TBE

(22) Visualizing DNA single- and double-strand breaks in the Flash comet assay by DNA polymerase-assisted end-labellingNucleic Acids ResearchFebruary 28, 2024Erik Bivehed, Björn Hellman, Leonie Wenson, Bo Stenerlöw, Ola Söderberg, Johan HeldinNt.BsmAI (R012) was used and for double-strand breaks of the genomic DNA the restriction enzyme RsaI (R0167) was used. All enzymes were acquired from New England Biolabs (NEB, USA). Fluorescent stains and fluorescently

(23) Multi-level profiling of the Fmr1 KO rat unveils altered behavioral traits along with aberrant glutamatergic functionTranslational PsychiatryFebruary 20, 2024George Ntoulas, Charalampos Brakatselos, Gerasimos Nakas, Michail-Zois Asprogerakas, Foteini Delis, Leonidas J. Leontiadis, George Trompoukis, Costas Papatheodoropoulos, Dimitrios Gkikas, Dimitrios Valakos, et al.for Fmr1 KO rats. The PCR products were subjected to digestion using the NEB restriction enzyme RsaI (R0167L) in the respective buffer. The digestion was carried out for 3 h at 37 °C, and PCR products were loaded

(24) Genome sequence and characterization of a novel Pseudomonas putida phage, MiCathSci RepDecember 9, 2023James Jaryenneh, Joseph S. Schoeniger, Catherine M. Mageeneyno. R0108T), NdeI (NEB, catalog no. R0111S), SwaI (NEB, catalog no. R0604S), RsaI (NEB, catalog no. R0167S). Restriction enzymes digestions were prepared by combining 10 × NEB reaction buffer, 500 ng of MiCath

(25) Genomic analyses reveal three phylogenetic species and their evolutionary histories in the big-headed turtleiScienceAugust 18, 2023Shiping Gong, Chatmongkon Suwannapoom, Minh Le, Truong Quang Nguyen, Yan Ge, Yufeng Wei, Yangchun GaoWHP-1.17113.023 Premix Taq™,TaKaRa,Cat# RR901A Agarose G-10,Biowest,Cat# BY-R0100 RsaI,NEB,Cat# R0167L ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, DNeasy Blood

(26) Nanopore-Based Fingerprint Immunoassay Based on Rolling Circle Amplification and DNA FragmentationACS NanoMarch 28, 2023Xinqi Kang, Connie Wu, Mohammad Amin Alibakhshi, Xingyan Liu, Luning Yu, David R. Walt, Meni WanunuBeads Phi29 polymerase was purchased from Enzymatics (P7020-HC-L); restriction enzymes AluI (R0137S), RsaI (R0167S), ExoI (M0293S), ExoIII (M0206S), and dNTPs (N0447L) were purchased from New England Biolabs (NEB);

(27) Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysisScientific ReportsDecember 1, 2022Christian Warmt, Ceren Yaslanmaz, Jörg Henkelby using 20 µl purified LAMP product (approx. 2 µg DNA), 2 µl enzyme (10 U/µl; New England Biolabs; R0167S) and 5 µl associated buffer (New England Biolabs; B6004S). Reaction mixture was filled up with ddH2O

(28) Arsenic Pollution and Anaerobic Arsenic Metabolizing Bacteria in Lake Van, the World’s Largest Soda LakeLife (Basel)November 16, 2022Esra Ersoy Omeroglu, Mert Sudagidan, Erdal OgunBiolabs, New England Biolabs, Ipswich, MA, USA, R3101S), RsaI (CutSmart Biolabs, New England Biolabs, R0167S), and HaeIII (CutSmart Biolabs, New England Biolabs, R01108S) restriction enzymes according to manufacturer’s

(29) A missense variant in the nuclear localization signal of DKC1 causes Hoyeraal-Hreidarsson syndromeNPJ genomic medicineOctober 30, 2022Chu CM, Yu HH, Kao TL, Chen YH, Lu HH, Wu ET, Yang YL, Lin CH, Lin SY, Tsai MM, et al.digested with Hinf I (New England Biolabs, Cat. No: #R0155S) and Rsa I (New England Biolabs, Cat. No: #R0167L) restriction enzyme in 10X CutSmart® Buffer (NEB, Cat. No: #B7204S) at 37 °C overnight. The digested

(30) The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1Nature CommunicationsDecember 1, 2021Sabrina Dietz et al.in 80 µl total volume with 40 U HinfI (New England BioLabs, #R0155) and RsaI (New England BioLabs, #R0167), respectively. The digestion was incubated at 37 °C overnight and the next day additional 10 U of each

(31) Association of leukocyte telomere length with metabolic syndrome in type 2 diabetes mellitusJ Res Med SciJuly 31, 2021Xuemin Peng, Jiaojiao Huang, Sanshan Xia, Yan Yang, Kun DongUSA). Genomic DNA was digested by Hinf I (R0155 L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167 L, New England BioLabs). Agarose gel electrophoresis was used to isolate the digested DNA. Then, the

(32) Association of leukocyte telomere length with metabolic syndrome in type 2 diabetes mellitusJournal of Research in Medical SciencesJuly 31, 2021Xuemin Dong, Jiaojiao Huang, Jiaojiao Huang, Sanshan Yang, Yan YangUSA). Genomic DNA was digested by Hinf I (R0155 L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167 L, New England BioLabs). Agarose gel electrophoresis was used to isolate the digested DNA. Then, the

(33) Establishment and Maintenance of Gnotobiotic American Cockroaches (Periplaneta americana)Journal of Visualized ExperimentsJune 14, 2021Helen E. Dukes, Josey E. Dyer, Elizabeth A. Ottesenstock solution (32%),Sigma-Aldrich,269336, Petroleum jelly,Vaseline,n/a, RsaI,New England BioLabs,R0167,Includes CutSmart (digestion) buffer Secondary container,n/a,n/a,a plastic container with a lid (such

(34) Telomere erosion in human pluripotent stem cells leads to ATR-mediated mitotic catastropheThe Journal of cell biologyJune 7, 2021Vessoni AT, Zhang T, Quinet A, Jeong HC, Munroe M, Wood M, Tedone E, Vindigni A, Shay JW, Greenberg RA, et al.(10 mM Tris and 1 mM EDTA, pH 8.0). 10 µg extracted DNA was digested overnight with 30 U each RsaI (#R0167; New England Biolabs) and HinfI (#R0155; New England Biolabs) restriction enzymes and resolved on a

(35) Novel and Efficient Protocol for DNA Coating- Based Identification of DNA-Protein Interaction by Antibody-Mediated ImmunodetectionReports of Biochemistry and Molecular BiologyMarch 2, 2021Niranjan Chellathurai Vasantha, Johnson Retnaraj Samuel Selvan, Saravanakumar Venkatachalam, Jackson Durairaj Selvan Christyraj, Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India., Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, Tamilnadu, India.KH2PO4. DNA Coating solution (Thermo Scientific - Cat. No. 17250). Restriction enzymes (Rsa I [NEB - Cat. No. R0167S] and Hinf I [NEB - Cat. No. R0155S]). Primary Antibodies [anti-lamin A antibody (Sigma- Cat. No. L1293)

(36) Single cell imaging of human cancer xenografts using adult immune-deficient zebrafishNature ProtocolsSeptember 1, 2020Chuan Yan, Daniel Do, Qiqi Yang, Dalton C. Brunson, John F. Rawls, David M. LangenauM0273S)Nuclease-free H2O (Life Technologies, cat. no. AM9939)HinfI (NEB, cat. no. R0155S)RsaI (NEB, cat. no. R0167S)Cutsmart buffer (NEB, cat. no. B7204S)Ultrapure Agarose (Life Technologies, cat. no. 165005001)Tris-acetate-EDTA

(37) Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering ToolkitACS Synthetic BiologyMay 15, 2020Binhui Zhao, Pankaj Chaturvedi, David L. Zimmerman, Andrew S. BelmontDpnI, HaeIII, MseI, MspI, RsaI (New England Biolabs, Cat. # R0137S, R0176S, R0108S, R0525S, R0106S, R0167S, respectively) and CutSmart Buffer (New England Biolabs); Terminal Deoxynucleotidyl Transferase and

(38) Shorter leucocyte telomere length as a potential biomarker for nonalcoholic fatty liver disease-related advanced fibrosis in T2DM patientsAnnals of Translational MedicineMay 2, 2020Kun Dong, Ye Zhang, Jiao-Jiao Huang, San-Shan Xia, Yan YangNanodrop. Genomic DNA were digested by Hinf I (R0155L, New England Bio Labs, Beverly, MA, USA) and RsaI (R0167L, New England BioLabs) at 37 °C overnight. Then, agarose gel electrophoresis was applied to separate

(39) The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a MitochondrionMolecular Biology and EvolutionOctober 1, 2019Anna Karnkowska et al.in total volume 200 µl containing five units of the HinfI (NEB, R0155S) and five units of RsaI (NEB, R0167S) restriction enzymes. DNA was purified by ethanol precipitation and resuspended in 15 µl of nuclease-free

(40) Nej1 Interacts with Mre11 to Regulate Tethering and Dna2 Binding at DNA Double-Strand BreaksCell reportsAugust 6, 2019Mojumdar A, Sorenson K, Hohl M, Toulouze M, Lees-Miller SP, Dubrana K, Petrini JHJ, Cobb JA.K,Invitrogen,25530031 Raffinose,US Biological,R1030 RNase A,Sigma,R6513 RsaI,New England Biolabs,R0167S SDS,Avantor,409502 Sodium Acetate,VWR,BDH9278 Sodium Chloride,Fisher Chemical,S64212 Tris base,Fisher

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RsaI

RsaI from New England Biolabs

Sequence

New England Biolabs

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RsaI from New England Biolabs

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