RNasin Plus RNase Inhibitor from Promega

Product Specs
ItemRNasin Plus RNase Inhibitor
CompanyPromega
Price Get Pricing Get Quote for this Product
Catalog NumberN2615
Quantity10,000u
Concentration40 U/µL
SourceRecombinant E. Coli

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Endothelial ADGRF5(GPR116) governs vascular adaptation required for sustained thermogenic remodeling of brown adipose tissueMolecular MetabolismMay 1, 2026Rabih El-Merahbi, Vasiliki Karagiannakou, Ronja Kardinal, Lea Seep, Richard Lindner, Michelle Ynonne Jäckstein, Staffan Hildebrand, Mersiha Hasic, Eylül Korkmaz, Ankush Kumar Jha, et al.2 mL per 200 mg of tissue, supplemented with 10,000 units of RNasin® Plus RNase Inhibitor (Promega, N2615). Tissue homogenization was carried out using the gentleMACS™ Dissociator device, running a 5-minute

(2) Technical and biological sources of noise confound multiplexed enhancer AAV screeningNat CommunApril 28, 2026Avery C. Hunker, John K. Mich, Naz Taskin, Amy Torkelson, Trangthanh Cardenas, Jean-Benoît Lalanne, Joseph T. Mahoney, Darren Bertagnolli, Anish Bhaswanth Chakka, Rushil Chakrabarty, et al.P1171), 1x Protease Inhibitor Cocktail (Promega G6521), 0.2 U/µL RNasin Plus RNase Inhibitor (Promega N2615), 0.1% Triton X-100 dissolved in 0.22 μm filtered Nuclei Isolation Media. Previously frozen ALM or STR

(3) Single-Nucleus RNA Sequencing Reveals Muscle Fiber Cell Heterogeneity During Human Skeletal Muscle AgingAging CellApril 14, 2026Caixia Gong, Li Wu, Ange Wang, Nan Hua, Chengfan Qin, Shunmei Huang, Xia Zhang, Yunmei Yang, Jing Chen, Qin Zhang(NUC‐101; Sigma‐Aldrich) supplemented with protease inhibitor (5892791001; Roche) and RNase inhibitor (N2615; Promega and AM2696; Life Technologies) according to the standard protocol. Single‐nuclei suspensions

(4) Human antibodies against West Nile and related orthoflavivirusesbioRxivApril 6, 2026Tomás Cervantes Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.containing lysis buffer (0.5× PBS, 10 mM dithiothreitol, and RNasin ribonuclease inhibitors; Promega, N2615) using the FACSymphony S6 cell sorter (BD Biosciences) and the analysis performed with FlowJo software.

(5) Posttranscriptional reprogramming controls MASLD progression through chronic ER stress adaptationSci AdvApril 3, 2026Eulalia Belloc, Vittorio Calderone, Salvador Naranjo-Suarez, Lidia Mateo, Judit Martin, Florencia Malizia, Annarita Sibilio, Veronica Chanes, Marta Ramirez-Pedraza, M. Eugenia Delgado, et al.Thermo Fisher Scientific), SuperaseIn (15 μl/ml; AM2696, Thermo Fisher Scientific), RNasin (25 μl/ml; N2615, Promega), and cycloheximide (100 μg/ml)] and then sonicated for 5 min at low intensity with a Standard

(6) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekwere assembled (total volume 25 μL, including 12.5 μL WGL, 1 μL KAc, 0.5 μL RNasin (Promega, B2775-N2615)) and incubated for 2 hrs at 25°C at 300 rpm. Nluc/Fluc expression was detected as described before

(7) Transcription factor NFYA directs male meiotic entry by regulating accessible chromatin at meiotic promoters in miceThe EMBO JournalMarch 19, 2026Martin Säflund, Masomeh Askari, Atiyeh Eghbali, Mukhtar Mohamed Abdi, Dilay Deren Er, Ann-Kristin Östlund Farrants, Tianxiong Yu, Deniz M ÖzatamM (w/v) CaCl2, 10% (v/v) glycerol, 1× protease inhibitor cocktail (PIC), 0.4 U/μl RNasin [Promega; N2615]). Afterwards, 100.5 μl lysis buffer 2 (9 mM (w/v) Tris-HCl pH 7.5, 1.8 mM (w/v) MgCl2, 2.7 mM (w/v)

(8) A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3Noncoding RNA ResMarch 19, 2026Xiaoyang Cao, Juzheng Sui, Ge Shan, Xu Liummol/L EDTA, 1% NP-40, 0.1% SDS) supplemented with 200 units of RNasin Ribonuclease Inhibitors (Promega, N2615), 2 mmol/L DTT, and 1 × protease inhibitor cocktail (Transgen, DI111). The cell suspension was sonicated

(9) Lysine-specific histone demethylase 1a regulates nephron development and long-term transcriptional programmingJCI InsightMarch 9, 2026Nicola Wanner, Julia Keller, Nastassia Liaukouskaya, Geoffroy Andrieux, Sandra D. Laufer, Manuel Rogg, Tillmann Bork, Wei Liang, Fabian Braun, Fabian Haas, et al.(0.04 U/μL SUPERaseIN RNAse Inhibitor [AM 2696, Thermo Fisher]; 0.04 U/μL RNAsin Plus RNAse Inhibitor [N2615, Promega]) was added. The single nuclei suspension was filtered through a 30 μm strainer (04-004-2326,

(10) Reprogramming mRNA localization by targeted RNA-protein interferencebioRxivMarch 2, 2026Devon E. Mason, Diptankar Bandyopadhyay, Neal Jiwnani, Brigitte Meyer, Stavroula Milioptimized buffer (Promega) supplemented with 20mM DTT, 10mM MgCl2, 4mM NTPs, 40U/ul RNasin Plus (Promega; N2615) and 1% Triton X-100. Reaction was incubated for 3.5hrs at 37°C. Post reaction, the in vitro transcribed

(11) Vitamin B2 and B3 nutrigenomics reveals a therapy for NAXD diseaseCellFebruary 25, 2026Ankur Garg, Skyler Y. Blume, Helen Huynh, Alec M. Barrios, Onurkan O. Karabulut, Qian Zhao, Ayush D. Midha, Adam W. Turner, B. Vittorio Resnick, Xuewen Chen, et al.0.15 mM spermine, 1% Triton X-100) containing 1 mM RNase inhibitor (RNasin Plus, Promega, cat.no.N2611/N2615). Hemibrain samples were Dounce homogenized on ice using 10 strokes of glass Pestle A and 20 strokes

(12) HCR-Proxy resolves site-specific proximal RNA microenvironments at subcompartmental resolutionNucleic Acids ResearchFebruary 23, 2026Anja Trupej, Valter Bergant, Jona Novljan, Martin Dodel, Tajda Klobučar, Maksimiljan Adamek, Flora C Y Lee, Karen Yap, Eugene Makeyev, Boštjan Kokot, et al.PBS, and blocked with IF blocking buffer [0.3% Triton X-100 + 3% BSA + 10 U ml−1 Rnasin (Promega, cat# N2615)] for 1 h at room temperature. To visualize RNA labelled with DIG, we used DyLight® 594 anti-DIG antibody

(13) Cholinergic signaling modulates intestinal pathophysiology in a Drosophila model of cystic fibrosisPLOS GeneticsFebruary 17, 2026Elizabeth A. Lane, Afroditi Petsakou, Ying Liu, Weihang Chen, Mujeeb Qadiri, Yanhui Hu, Norbert Perrimonbuffer [250mM Sucrose, 10mM Tris pH8, 25mM KCl, 5mM MgCl, 0.1% Triton-X, 0.5% RNasin Plus (Promega, N2615), 50x protease inhibitor (Promega G6521), 0.1mM DTT]. Using 1ml dounce (Wheaton 357538), nuclei were

(14) Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatosNature NeuroscienceFebruary 17, 2026Jackson W. Mace, Sachin P. Gadani, Matthew D. Smith, Danny Galleguillos, Bong Gu Kang, Milton Roy, Meilian Liu, Benjamin Summers, Thomas Garton, Marjan Gharagozloo, et al.nuclei extraction buffer (Miltenyi, 130-128-024) with 0.2 U μl−1 RNasin Plus RNase Inhibitor (Promega, N2615). Tissue was lysed in a C tube (Miltenyi, cat. no. 130-093-237) on a gentleMACS automated dissociator

(15) Ultrafast and specific miRNA quantification via single-molecule fluorescence quenching kineticsCommunications BiologyFebruary 14, 2026Juyoung Kim, Sungchul Hohng90 U/µl Yeast Poly(A) Polymerase (74225Z25KU, Thermo Fisher Scientific), and 2 U/µl RNase inhibitor (N2615, Promega). The reaction was terminated by heating the mixture at 65 °C for 15 min. For quantification

(16) Cell-type-specific RNA polymerase II activity maps in intact tissues provide a gateway to mammalian gene regulatory mechanisms in vivoDevelopmental CellFebruary 11, 2026Gopal Chovatiya, Sean Y. Huang, Alex B. Wang, Philip Versluis, Chris K. Bai, Michael DeBerardine, Yu-Ching Liao, Judhajeet Ray, Abdullah Ozer, John T. Lis, et al.including SUPERase (ThermoFisher AM2696), Protector (Sigma-Aldrich, 3335402001) and RNasin™ Plus (Promega, N2615) RNase inhibitors. The samples were dounced twice both with tight and loose pestle, filtered (100-μm

(17) Cleavage of mRNAs by a minority of pachytene piRNAs improves sperm fitnessNatureFebruary 4, 2026Katharine Cecchini, Mina Zamani, Nandagopal Ajaykumar, Joel Vega-Badillo, Ayca Bagci, Shannon Bailey, Phillip D. Zamore, Ildar GainetdinovLysis buffer: 10 mM Tris-HCl (pH 7.5), 3 mM CaCl2, 2 mM MgCl2, 10% glycerol, 0.04 U µl–1 RNasin PLUS (Promega, N2615), and 1× protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma;

(18) Rapid elicitation of neutralizing Asn332-glycan-independent antibodies to the V3-glycan epitope of HIV-1 Env in nonhuman primatesNature ImmunologyFebruary 3, 2026Ignacio Relano-Rodriguez, Jianqiu Du, Zi Jie Lin, Margaret Kerwin, Marta Tarquis-Medina, Eduardo Urbano, Jiayan Cui, Meagan Watkins, Christy L. Lavine, Peng Zhao, et al.tergitol, (type NP-40, 70% in H2O, Sigma-Aldrich, NP40S-100ML), 0.6 U μl−1 of RNAse inhibitor (Promega, N2615) in nuclease-free water (Qiagen), and incubated at 65 °C for 3 min. cDNA was synthesized by reverse

(19) Unique territorial and compartmental organization of chromosomes in the holocentric silkwormThe EMBO JournalFebruary 2, 2026José Gil, Emily Navarrete, Clio Hockens, Neil Chowdhury, Sameer Abraham, Gaétan Cornilleau, Elissa P Lei, Julien Mozziconacci, Edward J Banigan, Leah F Rosin, et al.Standard Taq Buffer,New England BioLabs,Cat#M0273 RNasin® Plus Ribonuclease Inhibitor,Promega,Cat#N2615 Maxima™ H Minus Reverse Transcriptase,Thermo Scientific™,"Cat#EP0751, EP0752, or EP0753" Corning 22×22

(20) Hepatic zonation determines tumorigenic potential of mutant β-cateninNatureJanuary 15, 2026Alexander Raven, Kathryn Gilroy, Hu Jin, Joseph A. Waldron, Holly Leslie, June Munro, Holly Hall, Rachel A. Ridgway, Catriona A. Ford, Doga C. Gulhan, et al.hybridization buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA and 2 M NaCl), 1 µl RNasin plus ribonuclease inhibitor (N2615, Promega) and 0.5 µl biotinylated DNA oligo pool (Supplementary Table 1; 100 µM total DNA with rRNA_depl_1

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