Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemProtease Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price $149.00 Inquire
Catalog Number5871S
Quantity1 ml
Research AreaRelated Products
Clone100X
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Capsid and integrase play essential apposing roles in viral ribonucleoprotein assembly during HIV-1 core morphogenesisiScienceJuly 17, 2026Ahinsa Ranaweera, Jonathan R. Andino-Moncada, Sarah E. Dillon, Satya P. Singh, Scott M. Stagg, Alan N. Engelman, Christopher Aiken, Ashwanth C. Francis100 μg/mL digitonin in the presence of protease inhibitor cocktail (Cell Signaling Technology, Cat#5871 S) using the following mixture: 490 μL PBS, 5 μL of 10 mg/mL digitonin, and 5 μL of 100× protease inhibitors.

(2) Development and Characterization of a FRET-based Formin Tension Sensor in Living CellsbioRxivJuly 13, 2026Philip Bleicher, John A. Hammer, James R. Sellers, Anjelika Gasilinalysed in cell lysis buffer (CST cat# 9803) supplemented with a protease inhibitor cocktail (CST cat# 5871). Lysates were briefly sonicated and protein levels were quantified using BCA assay kit (Pierce cat#

(3) Patient-derived tau-seeded human neuronal chimeras recapitulate mature Alzheimer's tau pathology and uncover human-specific neuronal vulnerabilitybioRxivJuly 9, 2026Yanru Ji, Purba Mandal, Jiahui Liu, Wen Hu, Bryce David Colón, Changlin Wan, Raymond W Pan, Dongkai Guo, Lijia Zhang, Xingjian Gu, et al.T-PER tissue protein extraction reagent (ThermoScientific, 78510) supplied with protease inhibitor cocktail (Cell Signaling Technology, 5871). The samples were then centrifuged at 10,000 × g for 5 mins to pellet the tissue debris. The supernatant

(4) Neutrophil-derived S100A8/A9 impairs megakaryocyte maturation in immune thrombocytopeniaNature CommunicationsJune 24, 2026Jiaqian Qi, Meng Zhou, Jie Yin, Xiaofei Song, Yan Zhang, Haohao Han, Xueqian Li, Ziyan Zhang, Yaqiong Tang, Fei Yang, et al.buffer (Beyotime, P0013B) supplemented with 1× protease and 1× phosphatase inhibitor cocktails (CST, #5871). Lysates were sonicated briefly (two 5-second pulses) and centrifuged at 12,000 × g for 5 minutes at

(5) Blocking interaction of sclerostin loop3 with osteoblastic LRP4 counteracts bone loss without increasing arterial stiffness during mechanical unloadingJ Orthop TranslatJune 8, 2026Luyao Wang, Ning Zhang, Hewen Jiang, Xiaohui Tao, Yuan Ma, Zebing Hu, Chuanxin Zhong, Xin Yang, Sifan Yu, Huarui Zhang, et al.(Thermo Scientific, 89,901) supplemented with a Protease Inhibitor Cocktail (Cell Signaling Technology, 5871). The lysate was then centrifuged at 4000 rpm for 30 min at 4 °C and replaced with binding buffer (PBS

(6) A whole genomic CRISPR-Cas9 screen identifies the amino acid transporter SLC43A1 (LAT3) as a major determinant of oxaliplatin sensitivity in colorectal cancer cellsMol Cancer TherJune 2, 2026Nisha R. Pawar, Heidi M. Wade, Zoe Jackson, Nisha Poungpeth, Allison V. Mitchell, Connor P. Jewell, Dalen Chan, Pedro J. Batista, Robert W. Robey, Lisa M. Jenkins, et al.0.1% SDS, 2 mM EDTA, and 0.5% deoxycholate, 50 mM NaCl) containing protease inhibitor cocktail (cat# 5871, Cell Signaling Technology, Danvers, MA). Following sonication, samples were centrifuged at 5000 x g

(7) Incidence of B-cell Malignancies in Patients with Lung Cancer Receiving PD-1 Blockade TherapyClin Cancer ResMay 19, 2026Toshifumi Ninomiya, Caiyang Fang, Hirofumi Hamano, Teruya Morinaga, Wenhao Zhou, Toshihiro Koyama, Sakura Miki, Li Zhu, Yusuke Naoi, Daisuke Ennishi, et al.Tris–HCl (Nacalai Tesque; cat. #32739-31), protease inhibitor cocktail (Cell Signaling Technology; cat. #5871), and phosphatase inhibitor cocktail (Cell Signaling Technology; cat. #5870). Equal amounts of proteins

(8) Cardiolipin preserves Treg metabolic fitness and immune homeostasis in the gutNature MetabolismMay 18, 2026Annamaria Regina, Francesca Solagna, Malkon Sanchez Estrada, Maaike M. E. Jacobs, Daniel Martinez-Martinez, Theodoros Georgomanolis, Irma Alibashikj, Sara Gjurgji, Claire Pearson, Dehui Chang, et al.mM Na3VO4, 1 μg ml−1 leupeptin), supplemented with 1× Protease Inhibitor Cocktail (Cell Signaling, 5871) for 30 min, on ice, followed by centrifugation at 12,000g for 10 min, at 4 °C. Cleared protein lysate

(9) CtBP promoted P4HB mediates SLC7A11 maturation to confer ferroptosis resistance in triple-negative breast cancerJournal of Experimental & Clinical Cancer ResearchMay 16, 2026Lifen Wang, Hongxia Xu, Tianyu Liu, Dongliang Zhu, Liping Chen, Tzu-ming Liu, Shuhai Lin, Xueyi Liu, Hao Feng, Li Wang, et al.lysates were extracted by IP buffer (Thermo fisher, 87,788) with protease inhibitor cocktail (CST, #5871). The protein samples were collected from the supernatant after centrifugation at 12,000 g for 15 min

(10) Hierarchical small molecule inhibition of MYST acetyltransferasesNat CommunMay 13, 2026Xuemin Chen, Alexandra Castroverde, Minervo Perez, Ronald Holewinski, Kiall F. Suazo, Rashmi Karki, Thorkell Andresson, Benjamin A. Garcia, Jordan L. Meier114-056-101], 200 mM NaCl, 0.1% NP-40, and protease inhibitor cocktail [Cell Signaling Technology, 5871]). For each assay, 400 µL of diluted nuclear extract was centrifuged (20,000 × g, 4 °C, 30 min) to remove

(11) Gut-brain axis: beneficial impact of Shouchella clausii spores on fructose induced dysfunction is associated with modulation of the deoxycholic acid – TGR5 pathwayMolecular MedicineMay 9, 2026Maria Stefania Spagnuolo, Natasha Petecca, Francesca De Palma, Antonio Dario Troise, Angela Di Porzio, Valentina Barrella, Anella Saggese, Sabrina De Pascale, Marina De Stefano, Andrea Scaloni, et al.w/v SDS) containing 1% w/v Protease Inhibitor Cocktail and 1% w/v Phosphatase Inhibitor Cocktail (cat 5871 and cat 5870, respectively; Cell Signalling distributed by Euroclone, Milan, Italy). Homogenates were

(12) Destabilized calcium dynamics visualized using the genetically-coded probe GCaMPJ in intact hearts of Calstabin2-null miceFrontiers in PhysiologyApril 29, 2026Yixuan Liu, Youkun Bi, Jun Wang, Qi Yuan, Yu Rao, Fengchao Wang, Shiqiang Wang, Guangju Jihomogenized and lysed in cold RIPA buffer containing 1mM PMSF and protease inhibitor cocktail (Cat #5871; Cell Signaling Technology) for 30 min. The supernatant was collected after centrifugation at 12,000

(13) Thermally enhanced efficacy of chemotherapy potentiates cisplatin-based hyperthermic intraperitoneal chemotherapy in ovarian cancerScientific ReportsApril 24, 2026Fan Yang, Ofer Reizes, H. Petra Kok, Maarten F. Bijlsma, Constantijne H. Mom, Jacqueline M. Tromp, Willemien J. van Driel, Lukas J. A. Stalpers, Johannes Crezee, Arlene L. Oeiprepared using RIPA lysis buffer (89900, Thermo Scientific) supplemented with protease inhibitors (5871S, Cell Signaling) followed by sonication at 4 °C. Protein concentration was determined by BCA assay (23225,

(14) Osteoblastic sclerostin loop3-LRP4 interaction required by sclerostin to inhibit bone formationBone ResApril 24, 2026Luyao Wang, Xiaohui Tao, Hewen Jiang, Shijian Ding, Ning Zhang, Xin Yang, Shenghang Wang, Yihao Zhang, Nanxi Li, Haitian Li, et al.RIPA Lysis and Extraction Buffer (Thermo Scientific, 89901) supplemented with Protease Inhibitor Cocktail (CST, 5871) and centrifuged at 4 000 r/min for 30 min at 4 °C and replaced with binding buffer (PBS buffer, 20

(15) Circ72688 Drives Breast Cancer Invasion and Metastasis via the miR-654-5p/ORAI2 AxisOncology ResearchApril 22, 2026Haojie Yang, Zicong Tan, Zihao Liu, Kang Chen, Ning Liufu, Fengtao Jion ice using RIPA buffer (P0013B, Beyotime, Shanghai, China) supplemented with protease inhibitors (5871S, Cell Signaling Technology, Massachusetts, USA) for 30 min. Lysates were centrifuged at high speed,

(16) Allelic variation alters expression and antigen presentation of MR1 allomorphsJournal of Biological ChemistryApril 17, 2026Adam G. Nelson, Victoria Letoga, Clarice Z.Q. Lee, Carolyn Samer, Songyi Li, Lucy J. Meehan, Hamish E.G. McWilliam, Nicholas A. Gherardin, Allison Abendroth, Jose A. Villadangos, et al.Tris-HCl (pH 7.4), 5 mM MgCl2 with Protease Inhibitor Cocktail (Cell Signalling Technologies Cat No. 5871). Lysate was centrifuged at 13000g to separate the nuclear fraction, and total protein was quantified

(17) Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancerNat CommunApril 15, 2026Yapeng Su, Chunmei Liu, Xiang Lu, Hui-Yu Chuang, Guideng Li, Shiqun Shao, Yan Kong, Jihoon W. Lee, Rachel H. Ng, Stephanie Wong, et al.1 mL ice-cold PBS supplemented with 1X protease inhibitor cocktail (Cell Signaling Technology, Cat# 5871) and centrifuged. The cell pellets were resuspended in a cell lysis buffer containing 50 mM Tris-HCl

(18) The exonic SNP rs11676272-C risk allele mediates diet-induced obesity and reduces enhancer activationEMBO ReportsApril 6, 2026Weina Wang, Yue Li, Sheng Dong, Yuwei Liu, Chenghang Guo, Yuzhe Su, Wei Tian, Xiaoyu Hu, Zhenshan WangWestern blot The samples were lysed in lysis buffer (P0013, Beyotime) with a protease inhibitor cocktail (100 x) (5871, Cell Signaling Technology) for 20 min on ice, followed by centrifugation at 12,000×g for 20 min at

(19) Transcriptomic Profiling Identifies TALAM1 and LINC00702 as HIV-1-Responsive lncRNAs in MicrogliaInt J Mol SciApril 4, 2026Victoria Rojas-Celis, Catalina Millan-Hidalgo, Izabela Mamede, Isidora Morales-Vejar, Isidora Pittet-Díaz, Diego Fernández-Rodríguez, Paulo P. Amaral, Helder I. Nakaya, Sebastián Reyes-Cerpa, Fernando Valiente-Echeverría, et al.TNF-α-stimulated, and HIV-1-infected microglia were resuspended in RIPA buffer (Cell Signaling, Danvers, MA, USA, #5871S). Protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific,

(20) Alzheimer’s disease pathology degrades an NMDA receptor-dependent spontaneous activity pattern in cortico-hippocampal circuitsNeuronMarch 30, 2026Robert Ellingford, Samuel S. Harris, Marten Kehring, Rikesh M. Rajani, Francesca Kar Wey Lam, David Graykowski, Dorothea Bӧken, Lindsay A. Welikovitch, Anita Khasnavis, Rhiannon Laban, et al.Optics,Cat#36-429 LED UV Spot Lamp,Intertronics,Part#IUV101 Protease Inhibitor cocktail,Cell Signaling,Cat#5871S cOmplete ULTRA protease inhibitor cocktail,Roche,Cat#05892970001 PhosSTOP phosphatase inhibitor cocktail,Roche,Cat#4906845001 Zirconium

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Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

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Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

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