SignalSilence ® NF-kappaB p65 siRNA I from Cell Signaling Technology

Product Specs
ItemSignalSilence ® NF-kappaB p65 siRNA I
CompanyCell Signaling Technology
Price $369.00 Inquire
Catalog Number6261S
Quantity300 µl
TargetNFkB-p65
NCBI Gene AliasesAIF3BL3, CMCU, NFKB3, p65
NCBI Full Gene NameRELA proto-oncogene, NF-kB subunit
Research AreaImmunology and Inflammation
Molecular Weight65
ReactivityH
ApplicationssiRNA
TypesiRNA

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Intestinal epithelial PIEZO1 regulates macrophage-to-myofibroblast transition via epithelial-derived TGF-α signaling in Crohn’s diseaseCell Communication and SignalingMay 28, 2026Jing Yang, Jinyan Jing, Ying Wang, Qing Zhao, Ni Ma, Danxia Liang, Zexiong Su, Yan Hu, Zhiling Li, Hanshi Zeng, et al.transiently transfected with SignalSilence® NF-κB P65 siRNA I (Cell Signaling Technology, USA; Cat. # 6261) or control siRNA (Cell Signaling Technology, USA; Cat. #6568) using Lipofectamine™ 3000 (Thermo Fisher Scientific

(2) IL-28A/IL-10Rβ axis promotes angiogenesis via eNOS/AKT signaling and AP-1/NF-κB/MMP-2 network by regulating HSP70-1 expressionJournal of Advanced ResearchJuly 1, 2025Jun-Hui Song, Byungdoo Hwang, Sung Lyea Park, Hoon Kim, Soontag Jung, Changsun Choi, Hwan Myung Lee, Seok-Joong Yun, Yung Hyun Choi, Eun-Jong Cha, et al.5′-CCUCGUGCCGUUCCAUCAGGUAGUU-3′ (sense) and 5′-CUACCUGAUGGAACGGCACGAGGUU-3′ (antisense). AP-1 (#6203) and NF-κB (#6261) siRNAs were obtained from Cell Signaling Technology (Danvers, MA, USA). Statistical analysis Statistical

(3) Repression of ZNFX1 by LncRNA ZFAS1 mediates tobacco-induced pulmonary carcinogenesisCell Mol Biol LettApril 10, 2025Sichuan Xi, Jigui Shan, Xinwei Wu, Haitao Wang, Mary R. Zhang, Shakirat Oyetunji, Hong Xu, Zuoxiang Xiao, Tuana Tolunay, Shamus R. Carr, et al.concentrations, the cells were harvested for additional analysis at the designated times. NFkB-P65 siRNA (#6261S /Cell Signaling) was used to knockdown NFkB in relative experiments. Arraystar Human lncRNA/mRNA expression analysis

(4) Interplay between B7–H3 and HLA class I in the clinical course of pancreatic ductal adenocarcinomaCancer LettersApril 1, 2024Giulia Cattaneo, Marco Ventin, Shahrzad Arya, Filippos Kontos, Theodoros Michelakos, Yurie Sekigami, Lei Cai, Vincenzo Villani, Francesco Sabbatino, Francine Chen, et al.(Thermo Fisher Scientific), and PDAC cells were transfected with a NF-kB p65 siRNA (Cell Signaling, #6261) or a control siRNA (Cell Signaling, #6201) at the concentration of 100nM. Lipofectamin 3000™ transfection reagent

(5) Targeting up-regulated cIAP2 in SOX10-deficient drug tolerant melanomaMolecular Cancer TherapeuticsSeptember 5, 2023McKenna Q. Glasheen, Signe Caksa, Amelia G. Young, Nicole A. Wilski, Connor A. Ott, Inna Chervoneva, Keith T. Flaherty, Meenhard Herlyn, Xiaowei Xu, Andrew E. Aplin, et al.(Dharmacon, Lafayette, CO, #D-003520-01), or siRNA targeting NFκB p65 (Cell Signaling, Danvers, MA, #6261) at a final concentration of 25 nM using Lipofectamine RNAiMAX (Invitrogen, San Diego, CA). Sequences

(6) Targeting up-regulated cIAP2 in SOX10-deficient drug tolerant melanomaMol Cancer TherSeptember 5, 2023McKenna Q. Glasheen, Signe Caksa, Amelia G. Young, Nicole A. Wilski, Connor A. Ott, Inna Chervoneva, Keith T. Flaherty, Meenhard Herlyn, Xiaowei Xu, Andrew E. Aplin, et al.(Dharmacon, Lafayette, CO, #D-003520-01), or siRNA targeting NFκB p65 (Cell Signaling, Danvers, MA, #6261) at a final concentration of 25 nM using Lipofectamine RNAiMAX (Invitrogen, San Diego, CA). Sequences

(7) RIP140-Mediated NF-κB Inflammatory Pathway Promotes Metabolic Dysregulation in Retinal Pigment Epithelium CellsCurrent Issues in Molecular BiologyNovember 21, 2022Zeli Guo, Yuli Shen, Jianwen Zhong, Zhuoyun Li, Qi Guo, Xiangchao Yao, Yandong Wang, Wenyu Wucontrol infected for 48 h before RNA or protein extracts. siRNA against human-specific p65-NF-κB (#6261) was purchased from CST. Cells were incubated for 48 h in transfection reagents containing 100 nmol

(8) Endothelial plasticity drives aberrant vascularization and impedes cardiac repair after myocardial infarctionNat Cardiovasc ResApril 1, 2022Huang M, Yang F, Zhang D, Lin M, Duan H, El-Mayta R, Zhang L, Qin L, Shewale SV, Pei L, et al.non-targeting siRNA (Qiagen, 1027280) or siRNAs targeting Snail (Thermo Fisher, s13185), NF-κB (Cell Signaling, 6261), HIF-1α (Thermo Fisher, 42840), or PDGFR-β (Thermo Fisher, s10242) using Lipofectamine 2000 (Thermo

(9) CD70 antibody-drug conjugate: A potential novel therapeutic agent for ovarian cancerCancer scienceSeptember 1, 2021Shiomi M, Matsuzaki S, Serada S, Matsuo K, Mizuta-Odani C, Jitsumori M, Nakae R, Matsuzaki S, Nakagawa S, Hiramatsu K, et al.4‐h transfection, and measurements were performed on day 7. For NFkB‐p65 knockdown, siRNA‐NFkB‐p65 (#6261, Cell Signaling Technology) against NFkB‐p65 was transfected as aforementioned. NFkB‐p65‐silencing cells

(10) Lysophosphatidic acid induces tumor necrosis factor‐alpha to regulate a pro‐inflammatory cytokine network in ovarian cancerThe FASEB JournalOctober 1, 2020Wei Wang, Jinhua Wu, Abir Mukherjee, Tianhai He, Xiang‐Yang Wang, Yibao Ma, Xianjun Fangof NF-κB RelA/p65 was transiently silenced with SignalSilence® NF-κB p65 siRNA from Cell Signaling (#6261, Danvers, MA). The siRNAs for LPA1 (s4450), LPA2 (s17525) and LPA3 (s24110) were obtained from Thermo

(11) Lysophosphatidic acid induces tumor necrosis factor alpha to regulate a pro-inflammatory cytokine network in ovarian cancerFASEB JAugust 26, 2020Wei Wang, Jinhua Wu, Abir Mukherjee, Tianhai He, Xiang-Yang Wang, Yibao Ma, Xianjun Fangof NF-κB RelA/p65 was transiently silenced with SignalSilence® NF-κB p65 siRNA from Cell Signaling (#6261, Danvers, MA). The siRNAs for LPA1 (s4450), LPA2 (s17525) and LPA3 (s24110) were obtained from Thermo

(12) TNFα promotes mucosal wound repair through enhanced platelet activating factor receptor signaling in the epitheliumMucosal immunologyJuly 1, 2019Birkl D, Quiros M, García-Hernández V, Zhou DW, Brazil JC, Hilgarth R, Keeney J, Yulis M, Bruewer M, García AJ, et al.a concentration of 10 μg/ml. p65 NF-κB and control siRNA were from Cell Signaling Technologies (cat 6261S and 6201) and used as suggested by the manufacturer. The NF-κB inhibitors Flavopiridol hydrochloride

(13) Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growthCell death & diseaseFebruary 7, 2018Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, et al.

(14) Jagged1 is Clinically Prognostic and Promotes Invasion of Glioma-Initiating Cells by Activating NF-κB(p65) SignalingCellular Physiology and BiochemistryJanuary 1, 2018Long Hai et al.nontargeting siRNA scramble control and siRNA targeting either NF-kB(p65) (SignalSilence® NF-κB p65 siRNA #6261, Cell Signaling) were transfected into glioma cells using Lipofectamine 3000 (Invitrogen) according

(15) MicroRNA-132 Negatively Regulates Palmitate-Induced NLRP3 Inflammasome Activation through FOXO3 Down-Regulation in THP-1 CellsNutrientsDecember 18, 2017Byeon HE, Jeon JY, Kim HJ, Kim DJ, Lee KW, Kang Y, Han SJ.synthesized (Bioneer, Daejeon, Korea). Human NF-κB siRNA was purchased from Cell Signaling (catalog no. 6261; Danvers, MA, USA). THP-1 cells or primary murine macrophages were transiently transfected with FOXO3

(16) Aspirin ameliorates cerebral infarction through regulation of TLR4/NF‑κB‑mediated endoplasmic reticulum stress in mouse modelMolecular Medicine ReportsOctober 25, 2017Xin Wang, Bin Shen, Dezhou Sun, Xiangyu CuiUSA). ELISA. The expression of PERK (cat. no DEIA‑XYA1959; Creative Diagnostics, USA), NF‑ΚB (cat no. JK‑(a)‑6261; JingkangBioscience, Shanghai, China), and e eIF2 α (cat no. 7952S; Cell Signaling Technology, Inc.,

(17) Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2Scientific reportsAugust 29, 2017Wallner C, Jaurich H, Wagner JM, Becerikli M, Harati K, Dadras M, Lehnhardt M, Behr B.

(18) SHP2 is induced by the HBx-NF-κB pathway and contributes to fibrosis during human early hepatocellular carcinoma developmentOncotargetApril 18, 2017Kang HJ, Chung DH, Sung CO, Yoo SH, Yu E, Kim N, Lee SH, Song JY, Kim CJ, Choi J.lowercase). siRNA transfection Hep3B cells were transfected with 25, 50 or 100 nmol/L siRNA targeting NF-κB (#6261, Cell Signaling) or with control siRNA (D-001210-01; GE Healthcare Dharmacon, Lafayette, CO) using Oligofectamine

(19) Inflammatory landscape of human brain tumors reveals an NFκB dependent cytokine pathway associated with mesenchymal glioblastomaCancer lettersApril 1, 2017Zanotto-Filho A, Gonçalves RM, Klafke K, de Souza PO, Dillenburg FC, Carro L, Gelain DP, Moreira JC.

(20) Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-κB PathwayPloS oneJanuary 1, 2016Wang XL, Zhou YL, Sun W, Li L.100 nM), ERK1/2 siRNA (Cat#6560), JNK siRNA (Cat#6232), p38 MAPK siRNA (Cat#6564) or NF-κB siRNA (Cat#6261) (all Cell Signaling Technology, Danvers, MA, USA; all 100 nM) for 48 h using MACSfectin Reagent (Cat#130098410,

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