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Polymerase Chain Reaction (PCR)
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Reverse Transcription 10X Buffer
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Reverse Transcription 10X Buffer
Reverse Transcription 10X Buffer
Reverse Transcription 10X Buffer from Promega
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Citations:
(5)
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Promega for
Reverse Transcription 10X Buffer
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Description
Compatible with AMV Reverse Transcriptase and Taq DNA Polymerase. Use to perform cDNA synthesis; use resulting reaction in PCR without purification or buffer exchange. Composition: 100mM Tris-HCl (pH 9.0), 500mM KCl, 1% Triton X-100
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Reverse Transcription 10X Buffer
Company
Promega
Price
$695.00
Supplier Page
View Company Product Page
Catalog Number
A3561
Quantity
1.4ml
Type
Buffer
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (5)
(1)
NEAT and tidy: a novel RT-PCR method for the independent quantification of the NEAT1_1 isoform
Biology Methods and Protocols
October 11, 2025
Nadine E Smith, Janni Petersen, Michael Z Michael
Reagent,Final concentration,Manufacturer,Cat #,Location 5X reverse transcriptase buffer,1X,
Promega
,
A3561
,"Madison, WI, USA" 10 mM dNTP,0.5 mM,
Promega
,
U1511
,"Waltham, MA, USA" 100 U/µl M-MLV RNase H minus
(2)
Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cells
Scientific reports
November 7, 2023
Respekta N, Pich K, Mlyczyńska E, Dobrzyń K, Ramé C, Kamiński T, Smolińska N, Dupont J, Rak A.
recombinant
ribonuclease inhibitor
, and
Moloney murine leukemia virus
reverse transcriptase (cat. no.
U1515
,
A3561
,
C1101
,
N2515
, and
M1705
, respectively,
Promega
, USA). Specific primers for
ITLN1
and the reference
(3)
Macrophage innate training induced by IL-4 and IL-13 activation enhances OXPHOS driven anti-mycobacterial responses
eLife
September 29, 2022
Lundahl MLE, Mitermite M, Ryan DG, Case S, Williams NC, Yang M, Lynch RI, Lagan E, Lebre FM, Gorman AL,
et al
.
Bioscience,Cat# BIO-38028,Primers for
cDNA synthesis
Other,Reverse Transcriptase Buffer,
Promega
,Cat#
A3561
,Buffer for
cDNA synthesis
Other,RNAseOUT,
Invitrogen
,Cat#
10777019
,RNAse inhibitor for
cDNA synthesis
Other,RPMI
(4)
MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2
Experimental & molecular medicine
July 1, 2022
Hu L, Xie X, Xue H, Wang T, Panayi AC, Lin Z, Xiong Y, Cao F, Yan C, Chen L,
et al
.
Materials i and j). For
RT-PCR
, cDNA was prepared from 1 mg RNA using
Reverse Transcription 10X Buffer
(
A3561
,
Promega
) and analyzed with SYBR Green Master Mix (SABiosciences) using a
Real-Time PCR system
(CFX-Connect,
(5)
A predicted operon map for Mycobacterium tuberculosis
Nucleic Acids Research
August 1, 2007
P. Roback, J. Beard, D. Baumann, C. Gille, K. Henry, S. Krohn, H. Wiste, M.I. Voskuil, C. Rainville, R. Rutherford
To maintain buffer continuity between
RT-PCR
reactions, reverse-transcription 10× buffer (
Promega
#
A3561
) was used in both reverse transcription and
PCR reactions
. For each POP to be tested, MIT's Primer3
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Reverse Transcription 10X Buffer from Promega
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$695.00
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