Prime-a-Gene Labeling System from Promega

Product Specs
ItemPrime-a-Gene Labeling System
CompanyPromega
Price $167.00Supplier Page View Company Product Page
Catalog NumberU1100
Quantity30 reactions
ApplicationsLabeling

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Cell-type-specific ablation of siRNAs by Arabidopsis RTL1 reveals a role of phloem companion cells in systemic post-transcriptional gene silencingNucleic Acids ResApril 23, 2026Martin Lacroix, Hayat Sehki, Agnès Yu, Ivan Le Masson, Violette Martinelli, Hervé Vaucheret(Machery-Nagel), and radiolabeled dCTP-P32 was incorporated with a Prime-a-gene Labeling System kit (U1100, Promega). Oligonucleotide probes were ordered from GenoScreen, and radiolabeled dATP-P32 was incorporated

(2) Transcriptome-wide analysis of Arabidopsis DICER-LIKE1 RNA substratesNucleic Acids ResearchJanuary 22, 2026Nicolas G Bologna, Alexis Sarazin, Gregory Schott, Antoine Bouet, Natalia P Achkar, Belen Moro, Florence Jay, Uciel Chorostecki, Emanuel A Devers, Olivier VoinnetAT1TE55560, AT4TE22770, and AT2TE22890 were used using Prime-a-Gene Labeling System (Promega, Ref. U1100) according to the manufacturer’s instructions. One hundred nanograms of DNA were diluted in nuclease-free

(3) A trypanosome-specific complex mediates late-stage processing of cytosolic LSU rRNANucleic Acids ResDecember 11, 2025Simona Amodeo, Mauro Fischli, Silke Oeljeklaus, Salvatore Calderaro, Bettina Warscheid, André Schneiderwith 32P-labeled α-dATP was done using the Klenow fragment (Promega; Prime-a-Gene Labeling System; #U1100), and the probing of the membranes was performed as described [51]. For analysis of the various LSU

(4) A Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of T. bruceiLife science allianceNovember 1, 2023Gerber M, Suppanz I, Oeljeklaus S, Niemann M, Käser S, Warscheid B, Schneider A, Dewar CE.,Thermo Fisher Scientific,# A-11008,"IFA (1:1,000)" Commercial assay or kit,Prime-a-Gene labelling kit,Promega,U1100,Radioactive labelling of Northern probes Commercial assay or kit,EZView Red Anti-c-myc affinity gel,Sigma-Aldrich,E6654,CoIP Commercial

(5) The telomerase reverse transcriptase elongates reversed replication forks at telomeric repeatsScience AdvancesMarch 22, 2023Armela Huda, Hiroshi Arakawa, Giulia Mazzucco, Martina Galli, Valentina Petrocelli, Stefano Casola, Lu Chen, Ylli Doksanimembrane (GE Healthcare, RPN203). Probes were labeled with the Prime-a-Gene Labeling system (Promega, U1100) using [α-32P]deoxycytidine triphosphate (dCTP) (3000 Ci/μmol; PerkinElmer, 1300000327). For the TSQ1

(6) TYMV and TRV infect Arabidopsis thaliana by expressing weak suppressors of RNA silencing and inducing host RNASE THREE LIKE1PLOS PathogensJanuary 25, 2023Hayat Sehki, Agnès Yu, Taline Elmayan, Hervé Vaucheret(Machery-Nagel) and radiolabeled dCTP-P32 were incorporated with a Prime-a-gene Labeling System kit (U1100, Promega). Oligonucleotide probes were ordered from GenoScreen and radiolabeled dATP-P32 were incorporated

(7) Enrichment of centromeric DNA from human cellsPLOS GeneticsJuly 19, 2022Riccardo Gamba et al.probe for Southern was produced by apha-32P-dCTP-labelling (Prime-a-Gene Labeling System, Promega cat #U1100) of a ~300 bp PCR product obtained with primers 5′-CAGAAACTTCTTTGTGATGTGTGC-3′ and 5’-GTTTTTATGGGAAGATATTTCCT-3’

(8) Comparative analysis of potential broad-spectrum neuronal Cre driversWellcome Open ResearchJuly 8, 2022Katie M Paton, Jim Selfridge, Jacky Guy, Adrian BirdNcoI–BamHI fragment of the 3′UTR of Mecp2 ( Guy et al., 2001)). The Prime-a-Gene Labelling System (Promega U1100) was used to label the probe with [α32]dCTP. Membranes were washed briefly with room temperature 2×

(9) Nuclear and cytoplasmic RNA exosomes and PELOTA1 prevent miRNA-induced secondary siRNA production in ArabidopsisNucleic Acids ResearchFebruary 22, 2022Maria L Vigh, Simon Bressendorff, Axel Thieffry, Laura Arribas-Hernández, Peter Brodersenhybridized O/N at 65°C to radioactively labeled 3′-CF probes produced with Prime-a-gene labeling kit (#U1100, Promega). The membranes were washed 3 times in 2× SSC (0.3 M NaCl, 30 mM sodium citrate), 0.1% SDS

(10) SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancersMolecular cellFebruary 17, 2022Barral A, Pozo G, Ducrot L, Papadopoulos GL, Sauzet S, Oldfield AJ, Cavalli G, Déjardin J.Kit,Illumina,Cat#FC-131-1024 Luciferase Assay System,Promega,Cat#E1500 Prime-a-Gene® Labeling System,Promega,Cat#U1100 EpiTect Hi-C Kit,Qiagen,Cat#59971 MinElute PCR Purification Kit,Qiagen,Cat#28004 DNA Clean & Concentrator-5

(11) Innate, translation-dependent silencing of an invasive transposon in ArabidopsisEMBO reportsFebruary 3, 2022Oberlin S, Rajeswaran R, Trasser M, Barragán-Borrero V, Schon MA, Plotnikova A, Loncsek L, Nodine MD, Marí-Ordóñez A, Voinnet O.crosslinked (Pall & Hamilton, 2008). Probes from PCR products were radiolabeled using the Prime‐a‐Gene kit (#U1100, Promega) in the presence of [α‐32P]‐dCTP (Hartmann Analytic) and oligo probes were radiolabeled by

(12) Relative efficiency of split-marker versus double-crossover replacement protocols for production of deletion mutants in strain PH-1 of Fusarium graminearumFungal Genetics ReportsApril 29, 2021Sladana Bec, Gabdiel E. Yulfo-Soto, Lisa J. Vaillancourtgel-purified and labeled with radioactive isotope P32 using the Prime-a-gene labeling system (Promega, cat. #U1100). Between 1 and 5 µg of the genomic DNA isolated from the fungal strains was digested with 20 U of the

(13) The N-terminal BRCT domain determines MCPH1 function in brain development and fertilityCell death & diseaseFebruary 1, 2021Liu X, Schneble-Löhnert N, Kristofova M, Qing X, Labisch J, Hofmann S, Ehrenberg S, Sannai M, Jörß T, Ori A, et al.indicated probes after labelling with α-32P dCTP (Hartmann Analytic) using Prime-a-Gene® Labelling System (#U1100, Promega), as per manufacturer’s protocol and the Amersham NICK Columns (108–0855–02, GE healthcare

(14) Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene ExpressionMolecular & cellular proteomics : MCPJanuary 1, 2021Wu T, Nance J, Chu F, Fazzio TG.[α-32P]dCTP (PerkinElmer; 3000 Ci/mmol; 10 mCi/ml, BLU013H100UC) using Prime-a-Gene Labeling System (Promega; U1100). Unincorporated dCTP was removed by Bio-Spin P-30 columns (Bio-Rad; 7326231). Radioactivity was determined

(15) Location specific annealing of miR-122 and other small RNAs defines an Hepatitis C Virus 5′ UTR regulatory element with distinct impacts on virus translation and genome stabilityNucleic Acids ResearchSeptember 18, 2020Rasika D Kunden, Sarah Ghezelbash, Juveriya Q Khan, Joyce A WilsonGAPDH separately. The radioactive DNA probes used were prepared using Prime-a-Gene Labeling System kit (U1100, Promega, Madison, WI, USA), and radiolabeled dCTP (PerkinElmer, Boston, USA). The probes were generated

(16) PDM4, a Pentatricopeptide Repeat Protein, Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis thalianaFrontiers in Plant ScienceAugust 11, 2020Xinwei Wang, Lirong Zhao, Yi Man, Xiaojuan Li, Li Wang, Jianwei Xiaolabeled with 32P. The labeled probes were obtained by using the Prime-a-Gene Labeling Kit (SGMB01-Promega-U1100, USA). The sequences of the primers were according to Du et al. (2017). All the analysis was performed

(17) Virus-host coexistence in phytoplankton through the genomic lensScience AdvancesApril 3, 2020Sheree Yau et al.BLU008H250UC) according to the manufacturer’s instructions (Prime-a-Gene kit, Promega, catalog no. U1100) for use as DNA probes. Dried gels were equilibrated in sufficient hybridization buffer [6× SSC, 5×

(18) Homologue replacement in the import motor of the mitochondrial inner membrane of trypanosomeseLifeFebruary 27, 2020Corinne von Känel, Sergio A Muñoz-Gómez, Silke Oeljeklaus, Christoph Wenger, Bettina Warscheid, Jeremy G Wideman, Anke Harsman, Andre Schneiderreagent,MCP12ΔI-myc (plasmid),PMID: 27991487,,, Commercial assay or kit,Prime-a-Gene labelling kit,Promega,U1100,Radioactive labelling of Northern probes,Radioactive labelling of Northern probes Commercial assay

(19) SUMO-Chain-Regulated Proteasomal Degradation Timing Exemplified in DNA Replication InitiationMolecular cellNovember 21, 2019Psakhye I, Castellucci F, Branzei D.28106 ProbeQuant G-50 micro columns,GE Healthcare,Cat# 28903408 Prime-a-Gene labeling system,Promega,Cat# U1100 "Invitrogen Bolt 4-12% Bis-Tris Plus Gels, 15-well",Thermo Fisher Scientific,Cat# NW04125BOX "L-Arginine:HCl

(20) The m⁶A pathway protects the transcriptome integrity by restricting RNA chimera formation in plantsLife science allianceJune 1, 2019Pontier D, Picart C, El Baidouri M, Roudier F, Xu T, Lahmy S, Llauro C, Azevedo J, Laudié M, Attina A, et al.GeneClean Turbo Kit (Cat. No. 111102400; MP Biomedicals), and labeled with the Prime-a-Gene Labeling System (Cat. No. U1100; Promega) according to the manufacturer's instructions. The hybridization was performed in the PerfectHyb

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