GoTaq qPCR Master Mix from Promega

Product Specs
ItemGoTaq qPCR Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA6001
Quantity5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 ActivatorInternational Journal of Stem CellsFebruary 28, 2028Changyeong Kim, Kyung Hwan Park, Soo-Been Jeon, A-Reum Han, Ji Yoon Lee, Young-sup Yoonquantitative (q) RT-PCR using specific primers (Supplementary Table S1), GoTaqⓇ qPCR Master Mix (Promega, A6001), and the QuantStudioTM 3 System (Thermo Fisher Scientific). The relative mRNA expression of target

(2) Gene expression adaptation of metastases to their host tissueiScienceAugust 21, 2026Luise Nagel, Marten Wenzel, Sascha Hoppe, Mohammad Karimpour, Patrick S. Plum, Abdossamad Hamoudi, Israt Jahan, Sarah Schmitt, Marek Franitza, Roger Wahba, et al."GoScript™ Reverse Transcription Mix, Random Primers",Promega,Cat# A2800 GoTaq® qPCR Master Mix,Promega,Cat# A6001 NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles),Illumina,Cat# 20028316 ,, Deposited data,Deposited

(3) Pasta, a Versatile Transcriptomic Clock, Maps the Chemical and Genetic Determinants of Aging and RejuvenationAdv Sci (Weinh)July 27, 2026Jérôme Salignon, Maria Tsiokou, Patricia Marqués, Enriqueta Rodríguez-Diaz, Hazel Ang, Federico Pietrocola, Christian G. Riedel(Bio‐Rad, #1725038). Quantitative real‐time PCR was performed using GoTaq PCR Master Mix (Promega, #A6002) in a QuantStudio 5 Real‐Time PCR System (ThermoFisher). The average expression of ACTB (for detection

(4) Annexin A6 controls multi-organelle contact site formation and endolysosomal positioning, and remodels the STARD3 interactomeiScienceJuly 17, 2026Marc Bernaus-Esqué, Yangjing Liu, Eva Prats, Josep M. Estanyol, Gemma Martin, Maria Calvo, Panagiota Areti Gigourtsi, Mai Khanh Linh Nguyen, Alejandra R. Álvarez, Silvana Zanlungo, et al.4368814). In a final volume of 20 μL real-time PCR Brilliant SYBRGreen GoTaq qPCR Master Mix (Promega, A6002), 10 μL cDNA (diluted 1:20) was used as a template for PCR analysis using the LightCycler system (Roche

(5) Navigating Human Astrocyte Differentiation: Direct and Rapid One-Step Differentiation of Induced Pluripotent Stem Cells to Functional Astrocytes Supporting Neuronal Network DevelopmentGliaJuly 15, 2026Imke M. E. Schuurmans, Annika Mordelt, Marta Guevara-Ferrer, Katrin Linda, Denise Duineveld, Sofia Puvogel, Marina P. Hommersom, Nicky Scheefhals, Lisa Rahm, Emma Dyke, et al.using iScript cDNA Synthesis Kit (Bio‐Rad; 1708891). GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR. The following program was used in the 7500 Fast Real Time PCR System

(6) HPV8-E6 drives coordinated transcriptional and epigenetic reprogramming of keratinocytesTumour Virus ResJuly 13, 2026Martin Hufbauer, Adnan Syed, Felix Bormann, Daniel Hasche, Manuel Rodríguez-Paredes, Pia Laine, Anni Honkimaa, Petri Auvinen, Eeva Auvinen, Baki AkgülLeon-Rot, Germany). Quantitative PCR (qPCR) was performed using the GoTaq-qPCR SYBRGreen master mix (A6002, Promega, Walldorf, Germany) and the Light-Cycler system (Roche). Samples were analyzed in duplicate

(7) Arginase-2 enhances monocyte-endothelial interaction through regulation of integrin and membrane receptor expression: role in atherogenesisJ Biomed SciJuly 13, 2026Guillaume Ajalbert, Yuejun Yao, Sophie Rohrer, Matteo Caretti, Santhoshkumar Sundaramoorthy, Anastasia Rigkou, Michael Stumpe, Xiu-Fen Ming, Duilio M. Potenza, Zhihong Yangthe manufacturer’s protocol. Real-time PCR reaction was performed with the GOTaq® qPCR Master Mix (A6001, Promega, Madison, USA) and CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, USA). The mRNA

(8) SenFlag gene signature identifies senescent cells in mouse and human tissues through a conserved core transcriptional programEMBO JJuly 6, 2026Abdullah Altulea, Sebastian Mackedenski, Jamil Nehme, Marco DemariacDNA kit from Applied Biosystems (#4368813). qPCR was performed using GoTaq DNA Polymerase (Promega A6002) and gene-specific primers. Relative gene expression was calculated using the ΔΔCt method and normalized

(9) Modelling DMC1 mediated homologous recombination repair in mouse embryonic stem cellsFront Cell Dev BiolJuly 3, 2026Aditya Mhaskar, Esther Sleddens-Linkels, Ivy Liang, Fiona Horne, Achmed Al-Kourdi, Johan A. Slotman, Wiggert A. Van Cappellen, Maarten W. Paul, Mehrnaz Ghazvini, Adriaan B. Houtsmuller, et al.2012) (Supplementary Table S1). qPCR was performed using GoTaq® qPCR Master Mix (Promega, cat. No. A6002). qPCR data was analysed using CFX Maestro Software version 2.2 (BioRad). Cell cycle and apoptosis

(10) Loss of heterozygosity exposes germline mutations in complex I and drives Warburg metabolism in oncocytic carcinoma of the thyroidSci AdvJuly 3, 2026Celia de la Calle Arregui, Anderson R. Frank, Kelvin Mun, Jiwoong Kim, Kuntal Majmudar, Justin A. Bishop, Cheryl Lewis, Yang Xie, David G. McFaddenanalyses. Quantitative PCR qPCR was performed in triplicate using the GoTaq qPCR Master Mix (Promega, A6001) in a total reaction volume of 10 μl, containing 3 ng of DNA or cDNA. For mtDNA quantification, the

(11) Hepatic inflammation after exposure to a mixture of low-dose arsenic and cadmium in a murine model of fatty liver diseaseToxicological SciencesJuly 1, 2026Nivetha K Subramaniam, Natascha Gagnon, Cynthia Guilbert, Rushmi Perinpanathan, Madelyn Abraham, Christophe Goncalves, Jaymie R Meliker, Sonia Del Rincon, Koren K MannApplied Biosystems 7500 Fast RT-PCR system (Life Technologies) and GoTaqPCR Master Mix (Promega, Cat No. A6001) were used. QPCR primers were designed and purchased from Integrated DNA Technologies (Table S1) (He

(12) Deciphering interferon functions in avian influenza using receptor knockout models in the natural hostElifeJune 26, 2026Mohanned Naif Alhussien, Hanna Kaisa Vikkula, Romina Klinger, Christian Zenner, Simon P Früh, Rashi Negi, Theresa von Heyl, Sabrina Schleibinger, Milena Brunner, Tom VL Berghof, et al.Mix,Promega,Cat#A5001,Used for cDNA synthesis. Commercial assay or kit,GoTaq qRT-PCR kit,Promega,Cat#A6002,Used for qRT-PCR gene expression analysis. Commercial assay or kit,Histopaque-1077,Sigma-Aldrich,Cat#10771,Used

(13) Cellular assembly and functional resilience of the mammalian RNA exosomeThe EMBO JournalJune 25, 2026Tsimafei Navalayeu, Nikolaus Beer, Monika Bebjaková, Robert W Kalis, Ulrich Hohmann, Karel Stejskal, Gabriela Krššáková, Nina Fasching, Veronika A Herzog, Niko Popitsch, et al.SuperScript III Reverse Transcriptase,Thermo Fisher Scientific,Cat#18080085 GoTaq qPCR Master Mix,Promega,Cat#A6002 RNAseOUT,Thermo Fisher Scientific,Cat#10777019 Anti-FLAG M2 magnetic beads,Millipore,Cat#M8823 Anti-HA

(14) Multilevel Genetic and Functional Assessment of an ARR3 Frameshift Variant in Early-Onset High MyopiaInt J GenomicsJune 20, 2026Yadi Li, Fengwu Teng, Qin Zhu, Shulan Cui, Wenfu Yang, Pengcheng Maresultant cDNA was diluted fivefold and subjected to real‐time PCR using the 2× GoTaq qPCR Master Mix kit (A6001, Promega, United States). The reactions were run on a real‐time PCR system with initial denaturation

(15) Distinct 2-phenylimidazo[1,2-a]pyridine derivatives that inhibit breast cancer cell proliferation identified as AHR ligandsiScienceJune 19, 2026Katrin Koellisch, Christine Blattner, Stefano Motta, Janine Wesslowski, Melanie Rothley, Simone Büchel, Savannah Sirounian, Ilenia Segatto, Hanna T. Weber, Julia Müller, et al.of DEGs were verify by quantitative RT-PCR experiments with the SYBR Green GoTaq PCR mix (Promega, A6002). Thermal stability measurements (nano-DSF) Thermal denaturation experiments of AHR complexes in the

(16) Constraints on the G1/S transition pathway may favor selection of multicellularity as a passenger phenotypeElifeJune 18, 2026Tom Louis Ducrocq, Damien Laporte, Bertrand Daignan-Fornierprotocol. Quantitative PCR amplifications were then performed with GoTaq qPCR Master Mix (Promega, A6001), using 50 ng of DNA extracted from co-cultures. The first set of primers was designed to amplify the

(17) Spatial and single-cell transcriptomics reveal a HIF-1α/NF-κB-driven hypoxia-induced senescence axis in BPH epitheliumInt J Biol SciJune 10, 2026Zheng Li, Shuai Hu, Zhifu Liu, Muqiu Zhang, Shengbin Chen, Wei Yu, Mingxia Ding, Yu Fan, Senmao Li, Jie Jinreverse-transcribed into cDNA. Quantitative real-time PCR was performed using GoTaq qPCR Master Mix (A6001; Promega, Madison, WI, USA) on an Applied Biosystems 7500 Fast Real-Time PCR System according to the

(18) A 3'UTR-derived small RNA modulates the life cycle of the cholera toxin-encoding filamentous phage, CTXϕProc Natl Acad Sci U S AJune 9, 2026Anne Lippegaus, James R.J. Haycocks, Eoghan O'Driscoll, Marcel Sprenger, Kerstin Thriene, Elke-Martina Jung, Malte Siemers, Sebastian Krautwurst, David C. Grainger, Kai Papenfortstandardizing the concentration before the qPCR was run. The GoTaq qPCR master mix was used for qPCR (Promega, #A6001). The relevant oligonucleotides used for qPCR are listed in SI Appendix, Table S3. Transcriptomic analysis

(19) Mitochondrial protection role of oxidoreductase-like domain containing 1 in myocardial cells under hypoxiaMedical Gas ResearchJune 1, 2026Yan Yan, Min Dong, Liuyang Tian, Chao Zhu, Xiaojing ZhaoCo., Ltd., Osaka, Japan, RR047A)9 and GoTaq qPCR master mix (Promega Corporation, Madison, WI, USA, A6001).10 The CFX96 touch real-time PCR detection system (Bio-Rad Laboratories, Inc., Hercules, CA, USA; C1000 Touch

(20) A STAT1/ETC/GBP1 axis represents a potential therapeutic target for noncommunicable granulomatous skin diseaseSci AdvMay 29, 2026Manuel Huerta Arana, Henning Klapproth, Michal A. Stanczak, Luisa Bopp, Karla Witschurke, Robert Seitz, Maria Lopéz Martinez, Jan Zamek, Sinika Henschke, Nisha Rana, et al.to the manufacturer’s protocol. cDNA, primers (table S13), and the GoTaq qPCR Master Mix (Promega, A6002) were loaded into an MicroAmp Fast Optical 96-Well Reaction Plate (Applied Biosystems, 4346907), and

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