GoTaq qPCR Master Mix from Promega

Product Specs
ItemGoTaq qPCR Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA6001
Quantity5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Induced Pluripotent Stem Cells derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 ActivatorInternational Journal of Stem CellsFebruary 28, 2028Changyeong Kim, Kyung Hwan Park, Soo-Been Jeon, A-Reum Han, Ji Yoon Lee, Young-sup Yoonquantitative (q) RT-PCR using specific primers (Supplementary Table S1), GoTaqⓇ qPCR Master Mix (Promega, A6001), and the QuantStudioTM 3 System (Thermo Fisher Scientific). The relative mRNA expression of target

(2) hY3 RNA binds and inhibits ribosomes and modulates the starvation-induced integrated stress responseNucleic Acids ResAugust 24, 2026Valentina Pecoraro, EunBin Kong, Puneet Sharma, Yulia Gonskikh, Marek Żywicki, Fabian Nagelreiter, Markus Schosserer, Katrin Freiburghaus, Jean-Marc Nuoffer, Johannes Grillari, et al.(Thermo Fisher Scientific, Cat#SO142). qPCR was performed using the GoTaq® qPCR Master Mix (Promega, Cat#A6002) on a Rotor-Gene Q system (QIAGEN), with 3–4 technical replicates for each experiment. Gene-specific

(3) Gene expression adaptation of metastases to their host tissueiScienceAugust 21, 2026Luise Nagel, Marten Wenzel, Sascha Hoppe, Mohammad Karimpour, Patrick S. Plum, Abdossamad Hamoudi, Israt Jahan, Sarah Schmitt, Marek Franitza, Roger Wahba, et al."GoScript™ Reverse Transcription Mix, Random Primers",Promega,Cat# A2800 GoTaq® qPCR Master Mix,Promega,Cat# A6001 NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles),Illumina,Cat# 20028316 ,, Deposited data,Deposited

(4) Therapeutic Gfral silencing via antisense oligonucleotides ameliorates cancer-associated cachexia and extends survival in tumor-bearing miceCell Reports MedicineAugust 18, 2026Hyun Jung Hong, Min Hee Lee, Minsung Park, Jung Tae Kim, Jaehun Kim, Sang-Hyeon Ju, Ju Hee Lee, Jong-Woo Sohn, Koeun Shong, Daiki Setoyama, et al.Transcription Kit, 1,000 reactions",Applied Biosystems,Cat# 4368813 GoTaq® qPCR Master Mix,Promega,Cat# A6001 Fast Green Dye (Fast Green FCF),Sigma-Aldrich,Cat# F7252 FSC 22 frozen section media,Leica Biosystems,Cat#

(5) Multiple 4q25 risk variants impair calcium homeostasis and compromise left atrial functionCardiovascular ResearchAugust 14, 2026Verónica Jiménez-Sábado, Carmen Tarifa, Oliver B Vad, Sergi Casabella-Ramón, Gabriel Manzano-Reche, Estefanía Lozano-Velasco, Cristina Aceituno, Sheila Caño-Carrillo, Jorge N Domínguez, Christian Paludan-Müller, et al.transcriptase enzyme. Real-time PCR was performed using 2 µL cDNA, 5 µL GoTaq® qPCR Master Mix (Promega, A6002 Madrid, Spain), and 1 µL of specific primer (2.5 µM). Reactions were conducted on a CFX384TM thermocycler

(6) Long-term daily light exposure boosts human retinal organoid differentiationCommun BiolAugust 12, 2026Edwin M. van Oosten, Wietske Kieboom, Anita D. M. Hoogendoorn, Stijn L. Berendsen, Julia Haerkens, Marieke M. E. van der Maden, Eva Hruba, Devin Veerman, Anja Bufe, Seba Almedawar, et al.to 5 ng/µL for qPCR analysis. The qPCR reaction was set-up using the GoTaq qPCR master mix (Promega, A6002), according to the manufacturer’s instructions. The final cDNA concentration was 1 ng/µL per reaction.

(7) Bone morphogenetic protein 4-associated mitochondrial dysfunction in diabetic nephropathy involves Parkin downregulationFront Clin Diabetes HealthcAugust 11, 2026Masanori Tamaki, Tatsuya Tominaga, Kazuhiro Hasegawa, Takahiro Kida, Shinji Miyakami, Ikuko Shimizu, Sumiyo Yamaguchi, Masanori Minato, Kojiro Nagai, Shu Wakinousing a real-time PCR system (MiniOpticon, Bio-Rad, Hercules, CA, USA) with GoTaq® qPCR Master Mix (#A6001, Promega) and specific primers. Relative mRNA expression levels were calculated by the ΔΔCt method using

(8) STING deficiency does not rescue short telomere-mediated aging phenotypes and longevity in TERC- or TERT-telomerase deficient miceCell RepAugust 6, 2026Rosa M. Marión, José Carlos González, Juana M. Flores, Elena Piñeiro-Yáñez, Rosa Serrano, Maria A. BlascoIllumina,Lexogen,Cat#191 iSCRIPT cDNA Advance synthesis kit,Bio-Rad,Cat# 1725037 Go-Taq qPCR master mix,Promega,Cat# A6002 ,, Deposited data,Deposited data,Deposited data ,, RNA-seq dataset,This paper,GEO: GSE309674 ,, Experimental models

(9) Aquamin, a marine derived multi-mineral, attenuates toll-like receptor-mediated inflammatory responses in macrophagesFront ImmunolAugust 4, 2026Emmanouil Lioudakis, Sakshi Hans, Denise M. O'Gorman, Shane O'Connell, Margaret Lucitt(Integrated DNA Technologies/Belgium). RT-PCR was carried out using SYBR green GoTaq DNA polymerase (Promega, A6002) on the Mx3000P QPCR System (Agilent Technology), 40 cycles at 95°C for 15 s, 62°C for 1 min, and 72°C

(10) Metastatic Cancers Exploit Vagal Sensory Neurons and Neural Repair Programs to Colonize New TissuesResearch SquareJuly 27, 2026Felipe Almeida de Pinho Zaninelli, Tiago Saraiva-Santos, Gustavo Gastã0 Davanzo, Alliny Cs Davanzo, Alliny Bastos, Jose C de Lima-Junior, Amanda Martins de Lima-Junior, Amanda Mendes-Pierotti, Kennedy K Mendes-Pierotti, Keneedy Kirichok, et al.manufacturer’s protocol. Quantitative PCR was performed using the GoTaq® qPCR and RT-qPCR System (Promega, Cat. #A6001). Each reaction contained 50 ng of cDNA template and was run in triplicate under standard cycling conditions.

(11) Pasta, a Versatile Transcriptomic Clock, Maps the Chemical and Genetic Determinants of Aging and RejuvenationAdv Sci (Weinh)July 27, 2026Jérôme Salignon, Maria Tsiokou, Patricia Marqués, Enriqueta Rodríguez-Diaz, Hazel Ang, Federico Pietrocola, Christian G. Riedel(Bio‐Rad, #1725038). Quantitative real‐time PCR was performed using GoTaq PCR Master Mix (Promega, #A6002) in a QuantStudio 5 Real‐Time PCR System (ThermoFisher). The average expression of ACTB (for detection

(12) Periductal Fibroblast Density Defines Lymphocyte Exclusion via a CD44-Dependent Stromal Checkpoint in Pancreatic CancerCancer Immunol ResJuly 24, 2026Zoe X. Malchiodi, Alexander A. Lekan, Robert K. Suter, Atul Deshpande, Selime Arslan, Annabel J. Lee, Nuan Wang, Ivana Peran, Brent T. Harris, Anju Duttargi, et al.Supplementary Table S5, along with primer sequences, was performed using GoTaq qPCR Master Mix (Promega, CAT#: A6001) with the StepOnePlus™ Real-Time PCR System (ThermoFisher). Gene expression was quantified (2−ΔCт) using

(13) Annexin A6 controls multi-organelle contact site formation and endolysosomal positioning, and remodels the STARD3 interactomeiScienceJuly 17, 2026Marc Bernaus-Esqué, Yangjing Liu, Eva Prats, Josep M. Estanyol, Gemma Martin, Maria Calvo, Panagiota Areti Gigourtsi, Mai Khanh Linh Nguyen, Alejandra R. Álvarez, Silvana Zanlungo, et al.4368814). In a final volume of 20 μL real-time PCR Brilliant SYBRGreen GoTaq qPCR Master Mix (Promega, A6002), 10 μL cDNA (diluted 1:20) was used as a template for PCR analysis using the LightCycler system (Roche

(14) Navigating Human Astrocyte Differentiation: Direct and Rapid One-Step Differentiation of Induced Pluripotent Stem Cells to Functional Astrocytes Supporting Neuronal Network DevelopmentGliaJuly 15, 2026Imke M. E. Schuurmans, Annika Mordelt, Marta Guevara-Ferrer, Katrin Linda, Denise Duineveld, Sofia Puvogel, Marina P. Hommersom, Nicky Scheefhals, Lisa Rahm, Emma Dyke, et al.using iScript cDNA Synthesis Kit (Bio‐Rad; 1708891). GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR. The following program was used in the 7500 Fast Real Time PCR System

(15) Arginase-2 enhances monocyte-endothelial interaction through regulation of integrin and membrane receptor expression: role in atherogenesisJ Biomed SciJuly 13, 2026Guillaume Ajalbert, Yuejun Yao, Sophie Rohrer, Matteo Caretti, Santhoshkumar Sundaramoorthy, Anastasia Rigkou, Michael Stumpe, Xiu-Fen Ming, Duilio M. Potenza, Zhihong Yangthe manufacturer’s protocol. Real-time PCR reaction was performed with the GOTaq® qPCR Master Mix (A6001, Promega, Madison, USA) and CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, USA). The mRNA

(16) HPV8-E6 drives coordinated transcriptional and epigenetic reprogramming of keratinocytesTumour Virus ResJuly 13, 2026Martin Hufbauer, Adnan Syed, Felix Bormann, Daniel Hasche, Manuel Rodríguez-Paredes, Pia Laine, Anni Honkimaa, Petri Auvinen, Eeva Auvinen, Baki AkgülLeon-Rot, Germany). Quantitative PCR (qPCR) was performed using the GoTaq-qPCR SYBRGreen master mix (A6002, Promega, Walldorf, Germany) and the Light-Cycler system (Roche). Samples were analyzed in duplicate

(17) SenFlag gene signature identifies senescent cells in mouse and human tissues through a conserved core transcriptional programEMBO JJuly 6, 2026Abdullah Altulea, Sebastian Mackedenski, Jamil Nehme, Marco DemariacDNA kit from Applied Biosystems (#4368813). qPCR was performed using GoTaq DNA Polymerase (Promega A6002) and gene-specific primers. Relative gene expression was calculated using the ΔΔCt method and normalized

(18) Modelling DMC1 mediated homologous recombination repair in mouse embryonic stem cellsFront Cell Dev BiolJuly 3, 2026Aditya Mhaskar, Esther Sleddens-Linkels, Ivy Liang, Fiona Horne, Achmed Al-Kourdi, Johan A. Slotman, Wiggert A. Van Cappellen, Maarten W. Paul, Mehrnaz Ghazvini, Adriaan B. Houtsmuller, et al.2012) (Supplementary Table S1). qPCR was performed using GoTaq® qPCR Master Mix (Promega, cat. No. A6002). qPCR data was analysed using CFX Maestro Software version 2.2 (BioRad). Cell cycle and apoptosis

(19) Loss of heterozygosity exposes germline mutations in complex I and drives Warburg metabolism in oncocytic carcinoma of the thyroidSci AdvJuly 3, 2026Celia de la Calle Arregui, Anderson R. Frank, Kelvin Mun, Jiwoong Kim, Kuntal Majmudar, Justin A. Bishop, Cheryl Lewis, Yang Xie, David G. McFaddenanalyses. Quantitative PCR qPCR was performed in triplicate using the GoTaq qPCR Master Mix (Promega, A6001) in a total reaction volume of 10 μl, containing 3 ng of DNA or cDNA. For mtDNA quantification, the

(20) Hepatic inflammation after exposure to a mixture of low-dose arsenic and cadmium in a murine model of fatty liver diseaseToxicological SciencesJuly 1, 2026Nivetha K Subramaniam, Natascha Gagnon, Cynthia Guilbert, Rushmi Perinpanathan, Madelyn Abraham, Christophe Goncalves, Jaymie R Meliker, Sonia Del Rincon, Koren K MannApplied Biosystems 7500 Fast RT-PCR system (Life Technologies) and GoTaqPCR Master Mix (Promega, Cat No. A6001) were used. QPCR primers were designed and purchased from Integrated DNA Technologies (Table S1) (He

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