Propidium Iodide Solution from Miltenyi Biotec

(1) CD4-directed nanoblades enable selective genome editing in CD4+ cells and HIV suppression in vitro and in vivoEMBO Molecular MedicineJuly 6, 2026Jolien Van Cleemput, Maaike De Cock, Rianne Verbeek, Elianne Burg, Bram Van den Eeckhout, Wojciech Witkowski, Willem van Snippenberg, Zoe Stylianidou, Emily Brugger Galetic, Mareva Delporte, et al.by flow cytometry on a MACSQuant® Analyzer 10 (Miltenyi Biotec). Propidium iodide (Miltenyi Biotec, #130-093-233) was included as a cell viability marker. Primary human CD4+ cells were transduced and analyzed in a

(2) Erastin-Induced Ferroptosis Overcomes Oxaliplatin Resistance in Colorectal Cancer CellsBiomolecules & TherapeuticsJuly 1, 2026Na Yeong Lee, Sang Hoon Joo, Si Yeong Seo, Seung-On Lee, Goo Yoon, Jin Woo Park, Jung-Hyun Shimwith 50 µg/mL RNase A (EN0531, Thermo Fisher Scientific) for 15 min at room temperature. PI solution (130-093-233, Miltenyi Biotec) was added directly before flow cytometry. DNA content was analyzed using a MACSQuant VYB flow cytometer

(3) Human neonatal MR1T cells have more diverse TCR repertoires but reduced bacterial recognition than adult MR1T cellsNature CommunicationsJune 29, 2026Dylan Kain, Wael Awad, G. W. McElfresh, Meghan Cansler, Gwendolyn M. Swarbrick, Kean Chan Yew Poa, Conor McNeice, Gregory Boggy, Katherine H. Rott, Megan D. Null, et al.package58. Limited Dilution Cloning (LDC) CBMC were stained with Propidium Iodide (Milteny Biotec, 130-093-233), MR1/5-OP-RU tetramer (NIH tetramer core), and an antibody cocktail of surface stains to sort out non-CD3+

(4) Atorvastatin Attenuates Human Cardiac Fibroblast Activation, with Associated Changes in GATA4/MEF2C and Selected Fibrosis-Related microRNAsInt J Mol SciMay 6, 2026Nikola Chomaničová, Adriana Adamičková, Zdenko Cervenak, Simona Valášková, Andrea Gažová, Jan Kyselovicfollowing the manufacturer’s instructions. Cell viability was assessed using propidium iodide (Cat. #130-093-233, Miltenyi Biotec). 4.3. MTT Assay HCFs (5000 per well) were seeded into a transparent 96-well plate

(5) A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial DysfunctionbioRxivJanuary 16, 2026Oliver B. Davis, Jennifer E. Kung, Sonnet S. Davis, Rajarshi Ghosh, Shan V. Andrews, Neal S. Gould, Jillian H. Kluss, Elliot Thomsen, Maayan Agam, John P. Coan, et al.supernatant aspirated. Pellets were resuspended in 50ul of FACS buffer with Propidium Iodide (Miltenyi 130–093-233) (1:200) and added to the bottom of the FACS tube without filtration. 50ul of CountBright Absolute Counting

(6) EMT-ciliary signaling in quasi-mesenchymal-stem-like cells drives therapeutic resistance and is a druggable vulnerability in triple-negative breast cancerEMBO molecular medicineOctober 1, 2025Tessier CE, Derrien J, Dupuy AMM, Pelé T, Moquet M, Roul J, Douillard E, El Harrif C, Pinson X, Le Gallo M, et al.C Hydrocortison,Sigma,H0888-5G MEGM,Lonza,CC-3150 Naonedins,This study, 3D CellTiter-Glo,Promega,G7570 Paclitaxel,Sigma,T7191 Propidium Iodide,Miltenyi Biotech,130-093-233 TrypLe Express,Thermo-Fisher,12605036 Trypsin-EDTA 1X,Thermo-Fisher,25300054 EurobioGreen qPCR Mix,Eurobio,GAEMMX02L Chromium

(7) Genetic and pharmacological targeting of nicotinic acetylcholine receptor action blocks tumor progression in mouse models of breast cancerThe Journal of ImmunologyAugust 1, 2025Matthew A Heard, Jin Qian, Sakeef Sayeed, Sereena Mechlowicz, Qingyang Zhang, Sudha Yeluri, Katie Pool, Ryan Yamane, Gerald P Morris, Brian P Eliceiri#130-095-926), respectively. Viable cells were identified by staining with propidium iodide (Miltenyi Biotec; #130-093-233) and incubated with the following antibodies from Miltenyi Biotec: CD11b clone REA592, CD11c clone REA754,

(8) Thapsigargin triggers a non-apoptotic, caspase-independent programmed cell death in basophilic leukaemia cellsCell Death DiscovJuly 8, 2025Philip Steiner, Korollus Melek, Ancuela Andosch, Lena Wiesbauer, Anna Madlmayr, Michelle Duggan, Hubert H. Kerschbaum, Susanna Zierlercollected and stained on ice for Annexin-V-APC (Biolegend, 640919) and Propidium Iodide (Miltenyi, 130-093-233) in Annexin V staining buffer including (in mM): 10 HEPES pH 7.4, 140 NaCl, 2.5 CaCl2. The cells were

(9) B cells modulate lung antiviral inflammatory responses via the neurotransmitter acetylcholineNat ImmunolApril 22, 2025Antonio Cembellin-Prieto, Zheng Luo, Heather Kulaga, Nicole Baumgarthsubmitted for FACS sorting on live cells (FACSAria) using propidium iodide (Miltenyi Biotec, cat. no. 130-093-233) and cells were concentrated at 1,000 cell μl−1 before submitting for 10× scRNA-seq. It was ensured

(10) Enhanced conversion of T cells into CAR T cells by modulation of the MAPK/ERK pathwayCell Rep MedFebruary 18, 2025Elham Adabi, Filippos T. Charitidis, Frederic B. Thalheimer, Mar Guaza-Lasheras, Colin Clarke, Christian J. Buchholz,Selleck Chemicals,Cat#S1021 deucravacitinib,medchemexpress,Cat#HY-117287 ulixertinib,medchemexpress,Cat#HY-15816 ravoxertinib,medchemexpress,Cat#HY-15947 selumetinib,medchemexpress,Cat#HY-50706 VX-11e,medchemexpress,Cat#HY-14178 Propidium Iodide,Miltenyi Biotec,Cat#130-093-233 Fixable viability dye eFluor 780,ebioscience,Cat#65-0865-18 ,, Critical commercial assays,Critical

(11) The emergence of DNAM-1 as the facilitator of NK cell-mediated killing in ovarian cancerFront ImmunolJanuary 6, 2025Rachel Pounds, Wayne Croft, Hayden Pearce, Tasnia Hossain, Kavita Singh, Janos Balega, David N. Jeevan, Sudha Sundar, Sean Kehoe, Jason Yap, et al.CD14 - ECD (Beckman Coulter B92391), CD19 - ECD (Beckman Coulter 6604551), PI - ECD (Miltenyi Biotec 130093233), NKp46 - PerCP-Cy5.5 (Biolegend 137610), CD56 - PE/Cy7 (Biolegend 318318), NKG2C - APC (Biolegend 250704),

(12) Development and Characterization of 3-Dimensional Cell Culture Models of Adrenocortical CarcinomaEndocrinologyDecember 4, 2024Sarah Feely, Nathan Mullen, Padraig T Donlon, Eileen Reidy, Ritihaas Surya Challapalli, Mariam Hassany, Anna Sorushanova, Eduardo Ribes Martinez, Peter Owens, Anne Marie Quinn, et al.assessed using Calcein-AM (Merck, catalog # 17783) (8 mM) and propidium iodide (Miltenyi Biotec, catalog # 130-093-233) (5 µg/mL) and visualized with confocal microscopy. Flow cytometry using Sytox blue (Invitrogen catalog

(13) LuLIPLEX: A Fast, Highly Sensitive, and Multiplexed Method for the Detection of IgE Against Major AllergensAllergyNovember 27, 2024Edouard Leveque, Cyprien Pecalvel, Natacha Casanovas, Sophie Goyard, Yves L. Janin, Thierry Rose, Benjamin Trouche‐Estival, Pol André Apoil, Marine Michelet, Laurent Guilleminault, et al.for 30 min at 37°C and 5% CO2. The cells were stained with propidium iodide (PI) (Miltenyi Biotec #130‐093‐233), and the surface levels of hIgE‐AF647 were determined by flow cytometry using a MACSQuant 10 flow cytometer

(14) ECRG4 mediates host response to cutaneous infection by regulating neutrophil recruitment and adhesion receptor expressionPLOS ONENovember 7, 2024Katie D. Pool, Gracie J. Hemmat, Robert A. Dorschner130-119-121) and anti-CD62L-PE-Vio770 (Miltenyi Biotec, 130-112-838). Propidium iodide (Miltenyi Biotec, 130-093-233) exclusion was used to determine viable cells. Flow cytometry and data analysis was performed on a Miltenyi Biotec

(15) 3D model for human glia conversion into subtype-specific neurons, including dopamine neuronsCell Reports MethodsSeptember 16, 2024Jessica Giacomoni, Andreas Bruzelius, Mette Habekost, Janko Kajtez, Daniella Rylander Ottosson, Alessandro Fiorenzano, Petter Storm, Malin ParmarPhenol red solution,Sigma-Aldrich,Cat# P0290 Poly-L-Ornithine,Sigma-Aldrich,Cat# P4957 "Propidium iodide, PI",Miltenyi Biotec,Cat# 130-093-233 Recombinant Human GDNF Protein,R&D Systems,Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein,R&D Systems,Cat#

(16) B cells modulate lung antiviral inflammatory responses via the neurotransmitter acetylcholineResearch SquareJune 26, 2024Nicole Baumgarth, Antonio Cembellin Prieto, Zheng Luo, Heather Kulagaabove and submitted for FACS-sorting on live cells (FACSAria) using Propidium Iodide (Miltenyi Biotec # 130-093-233) and cells were concentrated at 1000 cell/uL before submitting for 10X single cell RNA Sequencing. Cell

(17) Deregulated protein homeostasis constrains fetal hematopoietic stem cell pool expansion in Fanconi anemiaNature communicationsFebruary 29, 2024Kovuru N, Mochizuki-Kashio M, Menna T, Jeffrey G, Hong Y, Me Yoon Y, Zhang Z, Kurre P.and resuspended in 300 µL ice-cold PBS+ with 5 µL propidium iodide (PI) solution (Miltenyi Biotec, 130-093-233). After at least 5 minutes on ice, protected from light, samples were sorted for live Ter119-negative

(18) Role of mitochondria in the myopathy of juvenile dermatomyositis and implications for skeletal muscle calcinosisJournal of AutoimmunityJuly 1, 2023Bhargavi Duvvuri, Lauren M. Pachman, Payton Hermanson, Ting Wang, Richard Moore, Dennis Ding-Hwa Ding-Hwa Wang, Aaron Long, Gabrielle A. Morgan, Stephen Doty, Rong Tian, et al.APC-conjugated Annexin V (Biolegend Cat. No. 640920 and propidium iodide (Miltenyi Biotec Cat. No. 130–093-233). Briefly, adhered RH30 cells following trypsinization are transferred into a U-bottomed 96-well plate

(19) Expansion of circulating stem-like CD8(+) T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in miceNature communicationsApril 12, 2023Onyshchenko K, Luo R, Guffart E, Gaedicke S, Grosu AL, Firat E, Niedermann G.anti-mouse CD16/32 Fc receptor–blocking antibody (clone 2.4G2). When propidium iodide (PI; Miltenyi Biotec, 130-093-233) was used for T cell exclusion, it was added to the samples directly before measurement. The M8 tetramer-PE

(20) A protocol to detect human CD4+ T cell extracellular traps using scanning electron microscopySTAR ProtocolsMarch 1, 2023Nam Phuong N. Nguyen, Nicole Chizara Oparaugo, Kelsey Ouyang, George W. Agak,Fisher Scientific,50-753-3010 autoMACS Rinsing Solution,Miltenyi Biotec,130-091-222 Propidium iodide,Miltenyi Biotec,130-093-233 Reinforced Clostridial Medium,Oxoid,CM0149B "1× PBS, pH 7.4",Gibco,10010023 Ficoll-Paque Plus,Cytiva,GE17-1440-02 "Trypan Blue Solution, 0.4

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Propidium Iodide Solution from Miltenyi Biotec

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Propidium Iodide Solution from Miltenyi Biotec

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