A microtiter plate was coated over night with Mouse anti Bovine interferon-γ antibody, clone CC330 (MCA2112) at concentration of 5μg/ml in ELISA coating buffer (BUF030A) and recombinant bovine interferon gamma (PBP007) as a standard. After washing with ELISA Wash buffer (BUF031A) and blocking with ELISA BSA Block (BUF032A) samples were detected using biotinylated Mouse anti Bovine interferon-γ antibody (MCA1783B) at 5μg/ml followed by incubation with streptavidin:HRP conjugate (STAR5B) at 1/1000. Finally, TMB core HRP substrate (BUF056A) was applied for color development. Data is presented as the mean of three measurements.
A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in. After washing with and blocking with samples were detected using antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate at 1/1000. Finally was used as substrate. Data is presented as the mean of three measurements.
Bovine interferon-γ ELISAA microtiter plate was coated overnight with Mouse anti Bovine interferon γ antibody, clone CC330 (MCA2112) at concentration of 5μg/ml in ELISA coating buffer (BUF030A). After washing with ELISA wash buffer (BUF031A) and blocking with ELISA BSA Block (BUF032A) samples were detected using biotinylated Mouse anti Bovine interferon γ antibody, clone CC302 (MCA1783B) at 5μg/ml followed by incubation with HRP conjugated streptavidin (STAR5B) at 1/1000. Finally, TMB CORE (BUF056A) was used as substrate.Data is presented as the mean of three measurements.
Supplier Page from Bio-Rad for TMB CORE