PEG 8000 Powder, Molecular Biology Grade from Promega

Product Specs
ItemPEG 8000 Powder, Molecular Biology Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV3011
Quantity500g

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A Microphysiological HHT-on-a-Chip Platform Recapitulates Patient Vascular LesionsResearch SquareMarch 27, 2026Christopher C.W. Hughes, Jennifer Fang, Christopher Hatch, Jillian Andrejecsk, William Van Trigt, Damie Juat, Yu-Hsi Chen, Satomi Matsumoto, Abraham Leetested for mycoplasma contamination and then concentrated in 50% polyethylene glycol (PEG) 8000 (Promega V3011) for at least 72h at 4°C. All lentiviral batches were then tested using HUVEC to calculate concentration

(2) Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivoNature MethodsMarch 12, 2026Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, et al.(2021092403, Nichiga), methyl-β-cyclodextrin (M1356, TCI), agave inulin (agabe500, Nichiga), PEG8000 (V3011, Promega), PEG10000 (81280, Sigma-Aldrich), RM + stevia (dex-5-500m, Nichiga), resistant maltodextrin

(3) Multi-tissue transcriptomic aging atlas reveals predictive aging biomarkers in the killifishNat AgingMarch 3, 2026Emma K. Costa, Jingxun Chen, Ian H. Guldner, Lajoyce Mboning, Natalie Schmahl, Aleksandra Tsenter, Rahul Nagvekar, Man-Ru Wu, Patricia Moran-Losada, Louis-S. Bouchard, et al.agent per sample. The crowding agent was prepared by combining filtered 40% wt/wt PEG 8000 (Promega, V3011) 1:1 vol/vol with 5× TAPS-MgCl2 (3 ml of 0.5 M TAPS-NaOH pH 8.5 (Boston Bioproducts, BB2375) combined

(4) Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epibolyDevelopmentFebruary 1, 2026Haoyu Wan, Sifa Quibria, Ernest Iu, Sirma Damla User, Sergey V. Plotnikov, Ashley E. E. Bruceembryo. Samples showing heterozygous and homozygous genotypes were purified using PEG8000 (Promega, V3011) and sent for sequencing to validate the mutation. Sequencing was done by The Centre of Applied Genomics

(5) Compact bacterial recombination complexes drive efficient kilobase-scale knock-in in mammalian cellsNucleic Acids ResearchJanuary 22, 2026Yining Luo, Qin Jiang, Yuanhao Qu, Wenqing Li, Ruofei Liu, Yong Zhu, Yingpei Xie, Chuanyi Jiang, Chen Chen, Le Cong, et al.freeze-thaw cycles, and AAV particles were precipitated from the cell lysate using PEG8000 (Promega, Cat. No. V3011). The precipitate was treated with Benzo Nuclease (Vazyme, Cat. No. RM1022) and sodium deoxycholate,

(6) Dietary DNA Metabarcoding From Animal Fecal SamplesCurrent ProtocolsJanuary 20, 2026Rachel D. McConnell, Crinan Jarrett, Diogo F. Ferreira, Luke L. Powell, Alma L. S. Quiñones, Davide M. Dominoni, Andreanna J. WelchGeneRuler Ultra Low Range Ladder and NEB ladders work well. Materials PEG‐8000 powder (Promega, cat. no. V3011)5 M NaCl (Current Protocols, 2006)1 M Tris·HCl, pH 8.0 (Current Protocols, 2006)0.5 M EDTA, pH 8.0 (Current

(7) Tissue microenvironment dictates the state of human iPSC-derived endothelial cells of distinct developmental origin in 3D cardiac microtissuesiScienceOctober 17, 2025Xu Cao, Maria Mircea, Sara Cascione, Atoosa Amel, Theano Tsikari, Francijna E. van den Hil, Hailiang Mei, Katrin Neumann, Anna Alemany, Konstantinos Anastassiadis, et al.(Beckman Coulter, A63987). The beads were diluted 1:2 in bead binding buffer (20% PEG8000 (Promega, V3011) and 2.5M NaCl). RNA was eluted in 6.4 μL of nuclease-free water. Next, 9.6 μL of IVT mix containing

(8) Transcriptomic analysis of phage-mammalian cell interaction reveals diverse phage immunobarcodesiScienceOctober 17, 2025Caroline Munini Muema, Belinda Kibii, Mingyue Zhong, Xinfeng Li, Paulina Miernikiewicz, Heng Xue, Yiyao Wang, Raphael Nyaruaba, Krystyna Dąbrowska, Hongping Wei, et al.Escherichia coli O111:B4,Sigma-Aldrich,93572-42-0 RNase A,VWR International,A2760.0100 PEG8000,Promega,V3011 Polymyxin B Sulfate,Coolaber,1405-20-5 ,, Critical commercial assays,Critical commercial assays,Critical

(9) Zinc finger domains bind low-complexity domain polymersNat CommunOctober 16, 2025Naohiko Iguchi, Noriyoshi Isozumi, Yoshikazu Hattori, Tomohiro Imamura, Takeshi Yokoyama, Masatomo So, Hitoki Nanaura, Takao Kiriyama, Nobuyuki Eura, Minako Yamaoka, et al.were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). Polyethylene glycol 8000 (PEG 8000, V3011) was purchased from Promega (Madison, WI, USA). Protease Inhibitor Cocktail (S8830) was purchased from

(10) TGFβ1–TNFα-regulated secretion of neutrophil chemokines is independent of epithelial–mesenchymal transition in breast tumor cellsMolecular Biology of the CellOctober 1, 2025Shuvasree SenGupta, Erez Cohen, Joseph Serrenho, Kaleb Ott, Pierre A. Coulombe, Carole A. Parentreleased from the packaging cells were collected 48 h posttransfection and concentrated using PEG-8000 (V3011, Promega), and then used to infect M2 cells in the presence of 10 µg/ml polybrene. The clones expressing

(11) Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosisNat CommunAugust 25, 2025Susovan Sarkar, Hao-Yang Liu, Feng Yuan, Brandon T. Malady, Liping Wang, Eileen M. Lafer, Jessica Perez, Jon M. Huibregtse, Jeanne C. Stachowiak2914-HT-100MG, U-100H, and UC-310). PEG8K (Polyethylene glycol 8000) was acquired from Promega (Catalog #: V3011). Amine-reactive PEG (mPEG-succinimidyl valerate MW 5000) and PEG-biotin (Biotin-PEG SVA, MW 5000) were

(12) TGFβ1-TNFα regulated secretion of neutrophil chemokines is independent of epithelial-mesenchymal transition in breast tumor cellsMol Biol CellAugust 20, 2025Shuvasree SenGupta, Erez Cohen, Joseph Serrenho, Kaleb Ott, Pierre A. Coulombe, Carole A. Parentreleased from the packaging cells were collected 48 hrs post-transfection and concentrated using PEG-8000 (V3011, Promega), and then used to infect M2 cells in the presence of 10 μg/ml polybrene. The clones expressing

(13) Spatially resolved C1QC+ macrophage-CD4+ T cell niche in colorectal cancer microenvironment: implications for immunotherapy responseCell DiscovJuly 1, 2025Hangyu Zhang, Libing Hong, Zhen Dong, Shan Xin, Bo Lin, Jinlin Cheng, Weihong Tian, Bin Li, Jing Wang, Xiaoyan Liu, et al.48 and 72 h after transfection. The virus was concentrated using PEG virus precipitation (Promega, #V3011). In brief, the collected supernatant was pooled and spun down at 3000× g for 15 min to remove cell

(14) Dnmt3a overexpression disrupts skeletal muscle homeostasis, promotes an aging-like phenotype, and reduces metabolic elasticityiScienceApril 18, 2025Mamoru Oyabu, Yuto Ohira, Mariko Fujita, Kiyoshi Yoshioka, Runa Kawaguchi, Atsushi Kubo, Yukino Hatazawa, Hinako Yukitoshi, Huascar Pedro Ortuste Quiroga, Naoki Horii, et al.Cap,VIOLAMO,Cat# SEF125V Salt Active Nuclease (SAN),Sigma-Aldrich,Cat# SRE0015 PEG 8000,Promega,Cat# V3011 OptiPrep,Serumwerk Bernburg AG,Cat# 1893 Type 70 Ti,Beckman Coulter,Cat# 337922 OptiSeal Tube,Beckman

(15) High-throughput screening of human genetic variants by pooled prime editingCell GenomicsApril 9, 2025Michael Herger, Christina M. Kajba, Megan Buckley, Ana Cunha, Molly Strom, Gregory M. Findlaywere collected 2 days and 3 days post-transfection, before pooling and concentrating with PEG-8,000 (#V3011, Promega). Aliquots of concentrated lentivirus were stored at −80°C until used. Aliquots of lentivirus

(16) FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomicsNature ProtocolsNovember 1, 2024Seung Won Shin, Prakriti Mudvari, Shravan Thaploo, Michael A. Wheeler, Daniel C. Douek, Francisco J. Quintana, Eli A. Boritz, Adam R. Abate, Iain C. Clark)Proteinase K, molecular biology grade, 2 ml (New England Biolabs, cat. no. P8107S)PEG-8000 (Promega, cat. no. V3011)PEG-6000 (Alfa Aesar, cat. no. A17541)DTT (Sigma-Aldrich, cat. no. 43816–10ML)Betaine (Sigma-Aldrich,

(17) DEPICT-seq: Single-Cell Transcriptomic Analysis of Rare Cell Subsets Isolated via Nucleic Acid CytometryAnalytical ChemistryOctober 15, 2024Kaixuan Bao, Xiaochun Jiang, Hong-min Hu, Tiemin Liu, Jingwei Zhangbuffer (Vazyme, P401), 0.2 U/μL AceTaq DNA Polymerase (Vazyme, P401), 1% Tween-20, 2% PEG8000 (Promega, V3011), 0.1% Triton X-100 (Thermo Fisher, 85111), 1.2 μM forward primer, 1.2 μM reverse primer, and 0.6 μM

(18) Diversity of ribosomes at the level of rRNA variation associated with human health and diseaseCell GenomicsSeptember 11, 2024Daphna Rothschild, Teodorus Theo Susanto, Xin Sui, Jeffrey P. Spence, Ramya Rangan, Naomi R. Genuth, Nasa Sinnott-Armstrong, Xiao Wang, Jonathan K. Pritchard, Maria BarnaAM9722 TGIRT-III,InGex,Cat# TGIRT50 "Buffer Kit, RNase-free",Invitrogen,Cat# AM9010 PEG 8000,Promega,Cat# V3011 DTT (Dithiothreitol),Thermo Fisher Scientific,Cat# A39255 "Betaine, 5M Solution",Fisher Scientific,Cat#

(19) RNA interacts with topoisomerase I to adjust DNA topologyMolecular CellSeptember 5, 2024Mannan Bhola, Kouki Abe, Paola Orozco, Homa Rahnamoun, Pedro Avila-Lopez, Elijah Taylor, Nefertiti Muhammad, Bei Liu, Prachi Patel, John F. Marko, et al.Chloride,Sigma-Aldrich,Cat# 211273 γ-32P-ATP,PerkinElmer,Cat# BLU502H250UC PEG 8000,Promega,Cat# V3011 Phenol:Chloroform:Isoamyl Alcohol mixture 25:24:1,Sigma-Aldrich,Cat# 77617 "Ethanol, absolute (200

(20) Ubiquitin-driven protein condensation stabilizes clathrin-mediated endocytosisPNAS NexusSeptember 2, 2024Feng Yuan, Sadhana Gollapudi, Kasey J Day, Grant Ashby, Arjun Sangani, Brandon T Malady, Liping Wang, Eileen M Lafer, Jon M Huibregtse, Jeanne C Stachowiak2914-HT-100MG, U-100H, and UC-310). Polyethylene glycol 8000 (PEG 8000) was purchased from Promega (Catalog #: V3011). Amine-reactive PEG (mPEG-succinimidyl valerate MW 5000) and PEG-biotin (Biotin-PEG SVA, MW 5000) were

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