NADPH Regeneration System V9510 from Promega

Product Specs
ItemNADPH Regeneration System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV9510
Quantity1,000 assays

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) ISGylation-mediated stabilization of CYP4Z1 fuels breast cancer initiation and progressionExp Mol MedJuly 2, 2026Yin Yuan, Yu Lu, Xuedan Han, Yidong Zhang, Qianqian Guo, Hong Yao, Qiyi Yu, Manzhen Zhou, Xinting Huang, Hongqi Wang, et al.preincubated at 37 °C for 10 min, after which 25 μl of a 2× NADPH regeneration system (Catalog no. V9510, Promega) was added to each well. Once biotransformation was completed, an equal volume of Luciferin detection reagent

(2) Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass SpectrometryAnalytical ChemistryMay 5, 2026Mary Ashley Rimmer, Jingheng Wang, Tharindu A. Ranathunge, Zhe Shi, Yong Li, DongGeun Lee, Sergio C. Chai, Wenwei Lin, Nathaniel R. Twarog, Aseem Z. Ansari, et al.enzyme–substrate mixture and 10 μL of 2× NADPH regeneration system (Promega, Madison, WI, cat. no. V9510). The final concentrations of reaction components were as follows: 10 nM CYP3A4, 2 μM Nifedipine or

(3) Metabolic stability and effects of microsomal enzymes on the activity of the sonic hedgehog pathway inhibitor piperonyl butoxideToxicology in VitroMarch 1, 2026Kenneth S. Rivera-González, Cameron O. Scarlett, Robert J. LipinskiCYP/EZ013; Cypex), and CYP3A4 (Catalog No. CYP/EZ010; Cypex), NADPH regenerating system (Catalog No. V9510; Promega), and SHH ligand (Catalog No. 1845-SH;R&D Systems). Metabolic stability assays The protocol

(4) Design and optimisation of meta-substituted bis(arylsulfonamido)benzene inhibitors through a molecular hybridisation strategy targeting the Keap1-Nrf2 protein-protein interactionJ Enzyme Inhib Med ChemFebruary 6, 2026Sumi Lee, Ahmed R. Ali, Dhulfiqar Ali Abed, Longqin Hupreincubated at 37 °C for 5 min before initiating the reaction with an NADPH regeneration system (Promega, V9510). After incubation for 30 min at 37 °C, the metabolic reaction was quenched by adding ice-cold acetonitrile

(5) Fluvastatin suppresses breast cancer initiation and progression via targeting CYP4Z1Communications BiologyJanuary 12, 2026Huilong Li, Ying Chen, Wanjin Shi, Zheng Miao, Yu Lu, Xuedan Han, Haitao Chen, Yunnan Zhang, Miaomiao Niu, Shengtao Xu, et al.min in 96-well white plates. Catalysis was initiated by adding 25 μL of 2× NADPH regeneration system (V9510, Promega, Madison, WI, USA). Following 30 min biotransformation at 37 °C, equal volumes of fluorescein

(6) Comparative analysis of LJ-4378 and tirzepatide in mouse models of obesity and weight regainArchives of Pharmacal ResearchNovember 7, 2025Heeseong Kim, Seung Woo Kim, Cheoljun Choi, SungUg Joo, Gibae Kim, Minjae Kim, Eunsuk Yang, Jangwon Lee, Sukjae Chung, Lak Shin Jeong, et al.Aldrich, K1003), Nicotinamide adenine dinucleotide phosphate (NADPH) regeneration system (Promega, V9510), terfenadine as the internal standard (Sigma Aldrich, T9562), and 0.1 M potassium phosphate buffer

(7) Sd-021, derivatives of decursin, inhibits tumorigenesis in NSCLC by inhibiting the EGFR/STAT3 signaling pathwaySci RepJuly 2, 2025Hyun-Ha Hwang, Jeong-Hui Je, Hyeong-Chan Lee, Ji-Sung Yoo, Taehyoun Kim, Jung Hwan Choi, Jin Won Hong, Hae-In Lim, Ga Yoon Kim, Yun-Beom Sim, et al.at 37 °C, the reactions were initiated by addition of an NADPH-regenerating system (Promega, Cat. #V9510) and allowed to proceed for 15 min at 37 °C. The reactions were terminated with ice-cold acetonitrile

(8) Decoding the selective chemical modulation of CYP3A4Nature communicationsApril 10, 2025Wang J, Nithianantham S, Chai SC, Jung YH, Yang L, Ong HW, Li Y, Zhang Y, Miller DJ, Chen T.The reaction was initiated by adding 12.5 µL of 2 × NADPH Regeneration System (Promega, catalog no. V9510), and the plate was incubated for 25 minutes at room temperature. The final concentrations of reaction

(9) Development and Evaluation of Indole-Based Phospholipase D Inhibitors for Lung Cancer ImmunotherapyJournal of medicinal chemistryMarch 13, 2025Song D, Lim SH, Kim Y, Lee H, Kim T, Lim H, Min DS, Han G.Stability Verapamil (TRC, V125000) was used as a reference compound along with an NADPH Regeneration system (Promega, V9510), chlorpropamide (TRC, C424800) as an internal standard, potassium phosphate buffer (pH 7.4, Corning,

(10) Discovery of a Novel Multitarget Analgesic Through an In Vivo-Guided ApproachPharmaceuticals (Basel)February 3, 2025Guo Zhen, Nayeon Do, Nguyen Van Manh, Hee-Jin Ha, Hee Kim, Hyunsoo Kim, Kwanghyun Choi, Jihyae Ann, Jeewoo Leepre-incubated at 37 °C for 5 min, followed by the addition of NADPH Regeneration System solution (Promega, V9510; Madison, WI, USA) and further incubation at 37 °C for 30 min. To terminate the reaction, an acetonitrile

(11) Discovery of novel FGFR4 inhibitors through a build-up fragment strategyJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2024Jihyung Kim, Chang Gyun Im, Kyujin Oh, Ji Min Lee, Fatimah Al-Rubaye, Kyung Hoon MinSubsequently, the reaction was initiated by adding the NADPH Regeneration System solution (Promega, #V9510) and allowed to incubate for 30 min at 37 °C. To terminate the reaction, an acetonitrile solution containing

(12) Stability Evaluation and Pharmacokinetic Profiling of Vepdegestrant in Rodents Using Liquid Chromatography–Tandem Mass SpectrometryMoleculesAugust 27, 2024Hae-In Choi, Jinyoung Choi, Jin Woo Kim, Yoon Ha Lee, Kwan Hyung Cho, Tae-Sung Kooliver microsomes were purchased from Gentest® (Woburn, MA, USA). The NADPH regeneration system (cat. no. V9510; Promega, Madison, WI, USA), ammonium formate (cat. no. 25030S0401; Junsei Chemical, Tokyo, Japan),

(13) Synthesis of mono Cytochrome P450 in a modified CHO-CPR cell-free protein production platformScientific ReportsJanuary 13, 2024Jan Felix Knauer, Christian Schulz, Anne Zemella, Doreen A. Wüstenhagen, Ruben Magnus Walter, Jan-Heiner Küpper, Stefan Kubickh unless otherwise noted. The reaction temperature was set at 37 °C. The NADPH Regeneration System (V9510) was used for the supply of NADPH during the assay. Three control approaches were performed, one with

(14) MAVS integrates glucose metabolism and RIG-I-like receptor signalingNature communicationsSeptember 2, 2023He QQ, Huang Y, Nie L, Ren S, Xu G, Deng F, Cheng Z, Zuo Q, Zhang L, Cai H, et al.assay kit (#MAK015, Sigma-Aldrich, MO, USA). NADPH level performed using NADPH quantification kit (#V9510, Promega, WI, USA). NAD+/NADPH level were performed using NADP/NADPH quantification kit (#G9081, Promega,

(15) Artemisia extracts differ from artemisinin effects on human hepatic CYP450s 2B6 and 3A4 in vitroJournal of EthnopharmacologyNovember 15, 2022Ndeye F. Kane, Bushra H. Kiani, Matthew R. Desrosiers, Melissa J. Towler, Pamela J. WeathersPromega: P450-Glo™ CYP3A4 assay (V9002), P450-Glo™ CYP2B6 assay (V8322), NADPH regeneration system (V9510); from Sigma Aldrich: DMSO (D8418), rifampicin (R3501), quercetin (Q4951); from Cayman Chemical: artemisinin

(16) Butyrate acts through HDAC inhibition to enhance aryl hydrocarbon receptor activation by gut microbiota-derived ligandsGut microbesJanuary 1, 2022Modoux M, Rolhion N, Lefevre JH, Oeuvray C, Nádvorník P, Illes P, Emond P, Parc Y, Mani S, Dvorak Z, et al.assessed using P450-Glo™ CYP1A1 Assay (Promega; Cat#V8751) and NADPH regeneration system (Promega; Cat#V9510). Reactions were performed in duplicate with butyrate concentrations ranging from 0.1 mM to 10 mM for

(17) Characterization of the endogenous DAF-12 ligand and its use as an anthelmintic agent in Strongyloides stercoraliseLifeDecember 7, 2021Zhu Wang, Mi Cheong Cheong, Jet Tsien, Heping Deng, Tian Qin, Jonathan DC Stoltzfus, Tegegn G Jaleta, Xinshe Li, James B Lok, Steven A Kliewer, et al.kit,Nano-Glo Dual-luciferase kits,Promega,N1620, Commercial assay or kit,NADPH regeneration system,Promega,V9510, Commercial assay or kit,Microsome Isolation Kit,Abcam,ab206995, "Chemical compound, drug",Ketoconazole,Sigma,K1003, "Chemical

(18) Characterization of the endogenous DAF-12 ligand and its use as an anthelmintic agent in Strongyloides stercoralisElifeDecember 7, 2021Zhu Wang, Mi Cheong Cheong, Jet Tsien, Heping Deng, Tian Qin, Jonathan DC Stoltzfus, Tegegn G Jaleta, Xinshe Li, James B Lok, Steven A Kliewer, et al.kit,Nano-Glo Dual-luciferase kits,Promega,N1620, Commercial assay or kit,NADPH regeneration system,Promega,V9510, Commercial assay or kit,Microsome Isolation Kit,Abcam,ab206995, "Chemical compound, drug",Ketoconazole,Sigma,K1003, "Chemical

(19) Unraveling the Structural Basis of Selective Inhibition of Human Cytochrome P450 3A5Journal of the American Chemical SocietyNovember 10, 2021Jingheng Wang, Cameron D. Buchman, Jayaraman Seetharaman, Darcie J. Miller, Andrew D. Huber, Jing Wu, Sergio C. Chai, Efren Garcia-Maldonado, William C. Wright, Jude Chenge, et al.necessary cofactors were used as enzyme sources. The NADPH regenerating system (Promega, catalog no. V9510) was also required to initiate the reaction. All assays were performed in 384-well white assay plates.

(20) Liver-specific knockout of histone methyltransferase G9a impairs liver maturation and dysregulates inflammatory, cytoprotective, and drug-processing genesXenobioticaJune 3, 2019Hong Lu, Xiaohong Lei, Qinghao Zhangto determine Cyp3a activity using P450-Glo™ Assay for CYP3A4 (V8901) and NADPH regeneration system (V9510) from Promega (Madison, WI). Mouse Cyp3a11 and human CYP3A4 share similar substrate specificity, and

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NADPH Regeneration System

NADPH Regeneration System from Promega

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NADPH Regeneration System from Promega

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