C166 from ATCC

Product Specs
ItemC166
CompanyATCC
Price $95.00Supplier Page View Company Product Page
Catalog NumberCRL-2581
TypeCell Biology
SpeciesMus musculus
TissueYolk sac
Validated Species ReactivityMouse (Ms)
Cell Type (standardized)Feeder Cells
Cell Typeendothelial cell
Age12 days embryo
ApplicationsThe cell line should prove useful for the study of endothelial cell differentiation and for the determination of the mechanisms underlying the establishment of organ-specific endothelial cell heterogeneity. The cell line can support the stable proliferation of multipotential hematopoietic stem cells, thus generating adequate numbers of cells for study of the mechanisms involved in their subsequent development and differentiation

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Efficient in vivo targeting of the myocardial scar using Moloney murine leukaemia virus complexed with nanoparticlesThe Journal of PhysiologyFebruary 1, 2026Timo Mohr, Miriam Schiffer, Deepak Ramanujam, Esther Carls, Callum M. Zgierski‐Johnston, Thomas Kok, Pia Niemann, Caroline Geisen, Peter Kohl, Stefan Engelhardt, et al.(β‐ME; Sigma‐Aldrich, St Louis, MO, USA; #M3148) in tissue culture flasks. C166 mouse EC (C166; ATCC; #CRL‐2581) were maintained in DMEM, supplemented with 10% v/v FCS, 100 U mL−1 penicillin, 100 µg mL−1 streptomycin

(2) Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progressionMol CancerDecember 18, 2025Erik Rasbach, Laure Migayron, Anne Brandenburg, Praveen Singh, Christina Martins, Ali Kassem, Anastasia I. Karkempetzaki, Nadine Suessner, Zsofi Kulcsar, Jessica Riopedre, et al.lines as well as human umbilical vein (HUVEC, Cat# CRL-1730, RRID: CVCL_2959) and murine C166 (Cat# CRL-2581, RRID: CVCL_6581) endothelial cells were obtained from ATCC. All cell lines were used at low passage,

(3) Mechanisms and clinical translation of ICOS/ICOSL signaling pathway blockade in delaying vascular complications of type 2 diabetesDiabetol Metab SyndrAugust 13, 2025Haiyan Zhang, LiBo Ruan, Xuemei Fu, Jinwen Yu, Qingrong Ruan, Yiyu Li, Hongying Wang, Heng Shao, Haoran Dong, Jianglan Cui(Zeiss LSM 880). C166 cell culture and treatments The C166 mouse yolk sac endothelial cell line (ATCC® CRL-2581™) was cultured in DMEM (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS;

(4) Computer vision to predict cell seeding coverage in re-endothelialized mouse lungsSci RepJuly 19, 2025Joshua Paciocco, Ahmed Hasan, Jason Chan, Daisuke Taniguchi, Cristina Amon, Golnaz Karoubi, Aimy Bazylakdescribed in Daly et al.38. For sample re-endothelialization, five million mouse C166 endothelial cells (CRL2581, ATCC, Canada) were suspended in 20 mL of cell culture media containing 89% high glucose content Dulbecco’s

(5) Persistent Activation of Sphingosine-1-Phosphate Receptor 1 by Phytosphingosine-3,4-Cyclic Phosphate Ameliorates Sepsis by Inhibiting Hyperinflammation and Vascular HyperpermeabilityMedComm (2020)June 3, 2025Suhong Duan, Seung-Gook Kim, Jiaying Bao, Hyung-Jin Lim, Joon Woo Kim, Sung-Il Yoon, Young Jun Park, Sanuk Yun, Kye-Seong Kim, Hwa-Ryung Song, et al.of an MФ‐specific lyz promoter (lyz‐Cre). 4.10 Cell Culture and siRNA Transfection MYSECs (ATCC# CRL‐2581) and Raw 267.4 cells (ATCC# TIB‐71) from the American Type Culture Collection (Manassas, VA, USA) were

(6) HDAC/σ1R Dual-Ligand as a Targeted Melanoma TherapeuticPharmaceuticals (Basel)January 28, 2025Claudia Giovanna Leotta, Carla Barbaraci, Jole Fiorito, Alessandro Coco, Viviana di Giacomo, Emanuele Amata, Agostino Marrazzo, Giovanni Mario PitariCRL-1619™), human lung adenocarcinoma A549 (ATCC® CCL-185™) and mouse endothelial cell C166 (ATCC® CRL-2581™) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% FBS, whereas human skin fibroblast

(7) In vitro and in vivo approaches to assess atherosclerosis following exposure to low-dose mixtures of arsenic and cadmiumToxicology and Applied PharmacologyDecember 1, 2023Nivetha K. Subramaniam, Natascha Gagnon, Kiran Makhani, Nikola Kukolj, Michael H. Mouradian, Braeden H. Giles, Harinee Srikannan, Victoria Fruh, Jaymie Meliker, Gregory A. Wellenius, et al.and methods 2.1. Cell viability assay RAW 264.7 (ATCC-TIB-71) murine macrophages and C166 (ATCC CRL-2581) murine endothelial cells were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM) media at

(8) Nongenetic surface engineering of mesenchymal stromal cells with polyvalent antibodies to enhance targeting efficiencyNature CommunicationsSeptember 19, 2023Tenghui Ye, Xi Liu, Xianghua Zhong, Ran Yan, Peng Shifrom Biological Industries (catalog: 04-001ACS). Cell culture Vascular endothelial cells C166 (ATCC, CRL-2581) were cultured in DMEM high glucose medium containing 10% fetal bovine serum, and chronic myelogenous leukemia

(9) Microporous Hydroxyapatite-Based Ceramics Alter the Physiology of Endothelial Cells through Physical and Chemical CuesJournal of functional biomaterialsSeptember 5, 2023Usseglio J, Dumur A, Pagès E, Renaudie É, Abélanet A, Brie J, Champion É, Magnaudeix A.200 °C for 2 h (Memmert GmbH, Schwabach, Germany). 2.2. In Vitro Assays 2.2.1. Cell Culture C166 (CRL-2581, ATCC), immortalized endothelial cells of murine origin were routinely cultured in high-glucose (4.5

(10) Erythroid-transdifferentiated myeloid cells promote portal vein tumor thrombus in hepatocellular carcinomaTheranosticsJuly 31, 2023Wei-Hang Zhu, Jie Chen, Run-Kai Huang, Yuan Zhang, Ze-Xuan Huang, Xiu-Qing Pang, Bo Hu, Yang Yang, Xing Liwere conducted at pathogen-free facilities. Cell culture Hepa 1-6 (ATCC CRL-1830) and C166 (ATCC CRL-2581) cell lines were purchased from the American Type Culture Collection (ATCC). They were cultured in DMEM

(11) Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver FibrosisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)February 1, 2023Zhao P, Sun T, Lyu C, Liang K, Niu Y, Zhang Y, Cao C, Xiang C, Du Y.published protocol.[ 38 ] C166 cell line was purchased from American Tissue Culture Collection (ATCC) (CRL‐2581). Mouse macrophage cell line (Raw264.7) was purchased from ATCC (TIB‐71). These cells were cultured

(12) Construction of Fusion Protein for Enhanced Small RNA Loading to Extracellular VesiclesGenesJanuary 19, 2023Es-Haghi M, Neustroeva O, Chowdhury I, Laitinen P, Väänänen MA, Korvenlaita N, Malm T, Turunen MP, Turunen TA.embryonic kidney cell line HEK293T (ATCC: CRL-11268), mouse yolk-sac endothelial cell line C166 (ATCC: ATCC CRL-2581) and human retinal pigment epithelial cell line ARPE19 cells (ATCC: CRL-2302) were cultured in Dulbecco’s

(13) HDL functionality is dependent on hepatocyte stress defense factors Nrf1 and Nrf2Frontiers in physiologyJanuary 1, 2023Trites MJ, Stebbings BM, Aoki H, Phanse S, Akl MG, Li L, Babu M, Widenmaier SB.expression by endothelial cells was analyzed via incubating C166 endothelial cells (ATCC, catalog# CRL-2581) with ApoB-depleted plasma and TNFα. Briefly, 2.5 × 105 C166 cells were added to each well of a 24-well

(14) Common and distinct functions of mouse Dot1l in the regulation of endothelial transcriptomeFrontiers in cell and developmental biologyJanuary 1, 2023Yoo H, La H, Park C, Yoo S, Lee H, Song H, Do JT, Choi Y, Hong K.lentiviral particles. Mouse embryonic dermal LECs (C57-6064L, Cell Biologics) and embryonic yolk sac BECs (CRL-2581, ATCC) were used for viral transduction. Each cell line was maintained according to the manufacturer’s

(15) Targeted Inhibition of Matrix Metalloproteinase-8 Prevents Aortic Dissection in a Murine ModelCellsOctober 14, 2022Zhang C, Niu K, Ren M, Zhou X, Yang Z, Yang M, Wang X, Luo J, Shao Y, Zhang C, et al.esiRNA Transfection C166 cells, a widely used mouse endothelial cell line, were purchased from ATCC (CRL-2581) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10 % FBS and 1 % penicillin/streptomycin,

(16) Involvement of Pore Formation and Osmotic Lysis in the Rapid Killing of Gamma Interferon-Pretreated C166 Endothelial Cells by Rickettsia prowazekiiTropical Medicine and Infectious DiseaseAugust 1, 2022Jenifer TurcoL929 Cell Preparation 2.1.1. Cultured Cells and IFN-γ The mouse endothelial C166 cell line (ATCC CRL 2581) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and grown in high

(17) VEGF-A and FGF4 Engineered C2C12 Myoblasts and Angiogenesis in the Chick Chorioallantoic MembraneBiomedicinesJuly 23, 2022Donna C. Kennedy, Antony M. Wheatley, Karl J. A. McCullaghMaterials and Methods 2.1. Cell Culture C2C12 (ATCC, #CRL-1772) mouse myoblast cells and C166 (ATCC, #CRL-2581) mouse yolk sac endothelial cells were cultured in high glucose Dulbecco’s Modified Eagle medium with

(18) A Tead1-Apelin axis directs paracrine communication from myogenic to endothelial cells in skeletal muscleiScienceJuly 15, 2022Umji Lee et al.lines ,, C2C12 myoblast cell line,ATCC,Cat# CRL1772 RRID:CVCL_0188 C166 endothelial cell line,ATCC,Cat# CRL2581 RRID:CVCL_6581 EOMA endothelial cell line,ATCC,Cat# CRL2586 RRID:CVCL_3507 C166GFP endothelial cell

(19) Microenergy acoustic pulses promotes muscle regeneration through in situ activation of muscle stem cellsJournal of Orthopaedic ResearchJuly 1, 2022He Zhang, Hubert T. Kim, Brian T. Feeley, Guiting Lin, Tom F. Lue, Mengyao Liu, Lia Banie, Xuhui Liugene expression that related to myogenesis. Endothelial cell Endothelial cells were purchased from ATCC (ATCC® CRL-2581™) and cultured in Endothelial cell growth media (R&D system, CCM027). The cells were divided into Control

(20) Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem CellsFrontiers in Bioengineering and BiotechnologyJune 20, 2022Azmeer Sharipol, Maggie L. Lesch, Celia A. Soto, Benjamin J. Frisch(B6.SJL-Ptprc a Pepc c /BoyJ) (Jackson Laboratories, Strain# 002014). Cells C166 cell line (ATCC Cat# CRL-2581). Emulate Human Emulation System Chip S-1™ Pod™ Portable Module. Zoë™ Culture Module. Orb™ Hub Module. Equipment Cell culture

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C166 from ATCC

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