Random Primers from Promega

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ItemRandom Primers
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC1181
TargetRandom
Quantity20microg
Primer TypeRandom Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Genetic evidence and cross-species functional characterization implicate CNN2 in age-related macular degeneration susceptibilityProc Natl Acad Sci U S ASeptember 8, 2026Fei-Fei Cheng, Hao Mou, Zhenyu Liu, Chang-Jun Zhang, You-Yuan Zhuang, Zhen Wu, Xin-Ran Wen, Angli Xue, Xiao Zhang, Jian Yang, et al.transcribed into cDNA with M-MLV reverse transcriptase (Cat. M1705, Promega) and random primers (Cat. C1181, Promega). Then, 10 ng of cDNA was mixed with SYBR Green PCR Master Mix (Roche, Basel, Switzerland)

(2) Reduced NLRP3, AIM2 and NLRC4 inflammasome activity in cord blood of extremely preterm infantsFront PediatrSeptember 8, 2026Hannah Siedel, Kristin Hieronymus, Jan M. Niehues, Lev Grinstein, Susanne Russ, Avril A. B. Robertson, Matthew A. Cooper, Jennifer L. Winkler, Angela Rösen-Wolff, Stefan WinklerM1701) within 6 months of RNA isolation. 1 μg total RNA was mixed with 0.5 μL random hexamer (Promega, C1181; 10 µM) and 0.5 μL oligo dT primer (Promega, C1101; 10 µM) and filled up with H2O to 25 μL reaction

(3) TCR γδ cell-specific STAT5 gain of function induces a druggable chronic human immune dysregulationJ Hum ImmunSeptember 7, 2026Benedikt J. Meyer, José Pedro Loureiro, Vladimir Nosi, Robin Hupfer, Adhideb Ghosh, Fabio Poletti, Annaïse Jauch, Julia Hirsiger, Caroline Berkemeier, Ingmar Heijnen, et al.Concentrations of 100 ng/μl to 1 μg/μl RNA were used for cDNA synthesis. We annealed random primers (#C1181; Promega) at 70°C for 5 min. cDNA synthesis was done according to the Qiagen GoScript Reverse Transcription

(4) Embryonic thermal programming combined with fractionated feeding induces early activation of hepatic lipid storage pathways in mule ducksPoultry ScienceSeptember 1, 2026Laura-Lou Zwick, Joséphine Huot, Cécile Heraud, Marie Lasserre, Anne Surget, Karine Gontier, Jérôme Roy, Stéphane Panserat, Marianne Houssierµl RNase-free water. The reaction included Random Primers (Promega, Madison, WI; product reference: C1181, 0.2 µg/ml final), a PCR nucleotides mix (Promega, Madison, WI; product reference: C1145, 0.004 mM final),

(5) Micro- and nanoplastics remodel the autophagy-lysosomal axis and mitochondrial function in primary human monocytesFrontiers in ImmunologyAugust 26, 2026Stefania Pezzana, Martina Broggiato, Fiona Limanaqi, Elena Vezzoli, Silvia Zecchini, Luigia Valsecchi, Claudia Vanetti, Lara De Luca, Clara De Palma, Michela Bardini, et al.nuclease-free H2O (60%), Oligo(dT) primers (20%; #C110A, 500 μg/ml, Promega) and random primers (20%; #C1181, 500 μg/ml, Promega). The tubes were then placed back in the thermoblock at 70°C for 5 minutes. The

(6) Combined EP300 and CREBBP Expression Levels Regulate Human Trophoblast DifferentiationInternational Journal of Molecular SciencesAugust 25, 2026Fangxu Lin, Remco Keijser, Gijs Afink74136). First-strand cDNA was synthesized from 1 µg of total RNA using 500 ng random primers (Promega, C1181, Madison, WI, USA), 1× M-MLV Reverse Transcriptase Reaction Buffer (Promega, M531A), 200 U M-MLV Reverse Transcriptase

(7) Growth Plate Morphogens as Potential Modulators of Cellular Tissue Remodeling by Annulus Fibrosus CellsJOR SpineAugust 22, 2026K. Stradovnik-Poikilidis, M. Teunissen, F. M. Riemers, M. van Doeselaar, J. Foolen, F. Abinzano, M. A. Tryfonidou, S. J. P. Callens, K. ItoM‐MLV Reverse Transcriptase Kit (Invitrogen, #10338842, Waltham, MA, USA) and random primers (Promega, #C1181, Madison, WI, USA). RT‐qPCR was performed using SYBR Green Master Mix (Bio‐Rad, #170‐8887, Hercules,

(8) Syndecan-2 Enhances Invasive Motility in Colon Cancer Cells via a Laminin-332–Integrin α6β1 AxisJournal of Biological ChemistryAugust 17, 2026Subin Cho, Jisun Hwang, Eunhye Park, Hyeonju Yang, Masumi Matsunuma, Keisuke Hamada, Yuji Yamada, Yamato Kikkawa, Eok-Soo OhRNA was used to generate cDNA using AMV Reverse Transcriptase (Cat# M5108) and Random Primer (Cat# C1181) (both from Promega US). Aliquots of the resulting cDNAs were amplified using specific primers (Table

(9) Seedless 3' pairing enables miR-17 family miRNAs to seize let-7 target sitesNucleic Acids ResAugust 14, 2026Artur Laski, Anneke Brümmer, Matije Lucic, Elisa Federici, Iris Mestres-Pascual, Jochen Imig, Alexander Kanitz, Mihaela Zavolan, Luca F R Gebert, Ian J MacRae, et al.TaqMan MicroRNA RT Kit (Thermo Fisher Scientific, #4366596) with a 1:1 mix of random hexamer (Promega, #C1181) and oligo (dT)15 primers (Promega, #C1101). qPCR was performed using KAPA SYBR FAST qPCR master mix

(10) Postprandial dynamics of sensory receptor and neural mechanisms underlying feeding regulation in mule ducksPoultry ScienceAugust 1, 2026Marie Lasserre, Yan Fericelli, Cécile Heraud, Laura-Lou Zwick, Karel Sasso, Sandra Biasutti, Anne Surget, Marianne Houssier, Stéphane Panserat, Jérôme Roy, et al.SuperScript III Reverse Transcriptase (Invitrogen; 18080044), Random Primers (Promega, Madison, WI, USA; C1181), and PCR nucleotide mix (Promega, C1145). Negative controls (no-RT and no-template) were included to

(11) Identification of Enhancers and Transcription Factors Regulating Postnatal Growth of Skeletal Muscle in CattleBiology (Basel)July 29, 2026Pengcheng Lyu, Binod Pokhrel, Jianguo Zhao, Honglin Jiang[26,27]. 2.5. RT-qPCR and Data Analysis Reverse transcription was performed using random primers (catalog #C1181, Promega, Madison, WI, USA), IImProm-IIReverse Transcriptase (catalog #A3803, Promega, Madison, WI,

(12) RAD54L coordinates the nucleolar DNA damage response to maintain rDNA stabilityThe EMBO JournalJuly 23, 2026Ruofei Liu, Jiachen Xuan, Carmelo Cerra, Nyree Stojnic, Junqi Pan, Shalini S Chelliah, Shannon Mendez, Rhynelle Dmello, Karla J Cowley, Yangyi Zhang, et al.instructions. First-strand cDNA was synthesized from 100 ng RNA using 0.5 µg random hexamers (Promega, C1181), 10 mM dNTP, 1x SSIV buffer, 0.5 mM DTT, ribonuclease inhibitor 40 U, and SuperScript IV reverse transcriptase

(13) Retargeted adenoviruses for local IgA and CD47 blocker production as a novel cancer therapyEMBO Molecular MedicineJuly 7, 2026Mariya Chernyavska, K Patricia Hartmann, J H Marco Jansen, Niklas Baumann, Jonas Kolibius, Dominik Brücher, Theodora Kristoforus, Rens H W Peters, Lucas Huijs, Daphne Laarveld, et al.Scientific), and 1000 ng of RNA was used for cDNA synthesis. After incubation with random primers (#C1181, Promega) and deoxynucleotides (#N0447L, New England BioLabs) at 65 °C for 5 min, a mix of RNAse inhibitor

(14) Protocol for quantifying silent ribosome induction in yeast and mammalian cells using polysome profilingSTAR ProtocolsJune 19, 2026Sayanur Rahaman, Samuel Mondal, Nicole Schiffelholz, Attila BecskeiScientific,Cat#18090200 Nuclease Free Water,Thermo Scientific,Cat#AM9932 Random hexamers,Promega,Cat#C1181 oligodT15,Promega,Cat#C1101 KAPA SYBR Fast,Sigma Aldrich,Cat#KK4611 Tris-HCl pH 8.0,Thermo Scientific,Cat#AM9856 MgCl2,Thermo Scientific

(15) Protocol for massively parallel RNA assay combined with immunoprecipitation for high-throughput analysis of RNA-protein interactions in cellsSTAR ProtocJune 19, 2026Yu Hsuan Lee, John L. Rinn, Taeyoung Hwang,Thermo Scientific,R0861 Proteinase K,Invitrogen,AM2546 RNaseOUT,Invitrogen,10777019 Random Primers,Promega,C1181 "PBS (10X), pH 7.4",Quality Biological,119-069-131 "PBS, pH 7.4",Gibco,10010023 "Tris-HCl (1 M),

(16) Short- and Long-Term Chrono-Immune Consequences of Dim Light at Night Exposure in Male Mice at Different Life StagesClocks SleepJune 17, 2026Carlos A. Trujillo, Fernando Miranda, José Sarmientomanufacturer’s instructions. First-strand cDNA was synthesized using random primers (Promega® Cat. #C1181, Madison, WI, USA), a dNTP mix (dATP, dCTP, dGTP, dTTP Promega® Cat. #U1330, Madison, WI, USA), and

(17) Activation of AMPK as a therapeutic strategy for FBXL4-related mitochondrial DNA depletion syndromeEMBO Molecular MedicineJune 17, 2026Aniketh Bishnu, Juan Zapata-Muñoz, Robert W Taylor, Kei Sakamoto, Ian G Ganleymanufacturer’s instructions. About 1 μg of RNA was then converted to CDNA using Random primers (Promega #C1181) using the SuperScript III reverse transcriptase (Thermo Fisher #18080044) following the manufacturer’s

(18) Multi-omic screening identifies RBMXL3 as a primate-specific RNA-binding protein and candidate regulator of RNA metabolism in human spermatogenesisCellular & Molecular Biology LettersMay 27, 2026Agata J. Barszcz, Katarzyna Tutak, Joanna Zyprych-Walczak, Erik Dassi, Erkut Ilaslan, Małgorzata Dąbrowska, Agnieszka Malcher, Marta Olszewska, Dominik Cysewski, Agnieszka Nadel, et al.SuperScript IV Reverse Transcriptase kit (Thermo Fisher Scientific, 18090050) with the use of random primers (Promega, C1181). For quantitative polymerase chain reaction (qPCR) 10 ng of complementary cDNA was mixed with PowerUp SYBR Green Master Mix

(19) The RNA helicase DDX53 (CAGE) contributes to RNA metabolism in a human germ cell modelbioRxivMay 25, 2026Agata J. Barszcz, Katarzyna Tutak, Agnieszka Malcher, Małgorzata Dąbrowska, Joanna Zyprych-Walczak, Erik Dassi, Erkut Ilaslan, Marta Olszewska, Dominik Cysewski, Michał Hrab, et al.SuperScript IV Reverse Transcriptase kit (Thermo Fisher Scientific, 18090050) with the use of random primers (Promega, C1181) using 1 μg of total RNA for each reaction. Ten ng of cDNA was used for qPCR, performed with PowerUp SYBR Green Master Mix

(20) Macrophage-mediated refinement of the dural lymphatic regulates social behaviorNeuronApril 24, 2026Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu, et al.dNTP (10mM) (Promega, cat. U1515), Oligo DT (Promega, cat. C1101), and random primers (Promega, cat. C1181). The reverse transcription was performed with the SuperScript™ III Reverse Transcriptase kit (Invitrogen™,

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Random Primers from Promega

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