MspI from New England Biolabs

Product Specs
ItemMspI
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR0106L
Quantity25000 units
Concentration20-100 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/CGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Isotype-specific antibody secreting plasma cells harbor epigenetic and transcription factor architectures defining distinct cell lineagesJ ImmunolAugust 17, 2026Lou-Ella M M George-Alexander, Roshni Roy, Sakeenah L Hicks, Mansi Gupta, Christopher D Scharer, Jeremy M Bossdescribed.7 The DNA was split at a 60:40 ratio and digested overnight with either Mspl (New England BioLabs; R0106S) or Taql (New England BioLabs; R0149S), respectively. The digested DNA fragments were combined, purified,

(2) Defined bacterial consortium highlights the impact of intestinal bacteria on DNA methylation and tumorigenesisGenome BiolJuly 7, 2026Claudia Mercado-Rodriguez, Siddhi Chitre, Pyoung Hwa Park, Ye Yang, Anthony Pompetti, Raad Z. Gharaibeh, Jason O. Brant, Jean-Pierre J. Issa, Christian Jobin[61]. Briefly, the mouse genomic DNA samples were digested with 100 units of MspI endonuclease (NEB, #R0106L) at 37 °C for 4 h. Blunt ends were created using Klenow DNA polymerase (NEB, #M0212) by filling in 5’

(3) Inverse Restriction Site-Associated DNA Sequencing (iRAD-seq)BIO-PROTOCOLJanuary 20, 2026Peng Chen, Shen Zhou, Haonan Wang, Jiaqi Gu, Yongquan Li, Yuxiao Chang(New England Biolabs, catalog number: R0525S) 14. MspI 20 U/μL (New England Biolabs, catalog number: R0106S) 15. AluI 10 U/μL (New England Biolabs, catalog number: R0137S) 16. 2% agarose (Sage Science, catalog

(4) Inverse Restriction Site-Associated DNA Sequencing (iRAD-seq)Bio ProtocJanuary 20, 2026Peng Chen, Shen Zhou, Haonan Wang, Jiaqi Gu, Yongquan Li, Yuxiao Chang(New England Biolabs, catalog number: R0525S) 14. MspI 20 U/μL (New England Biolabs, catalog number: R0106S) 15. AluI 10 U/μL (New England Biolabs, catalog number: R0137S) 16. 2% agarose (Sage Science, catalog

(5) Dendrogenomic resilience mechanisms of two endangered Mexican sprucesPlant DiversityJanuary 1, 2026Carlos Alberto Segura-Sanchez, Javier Hernández-Velasco, José Villanueva-Díaz, Víctor Chano, José Ciro Hernández-Díaz, Eduardo Mendoza-Maya, Artemio Carrillo-Parra, Christian Wehenkeldescribed by Abed et al. (2019). Briefly, 200 ng of DNA per individual was double-digested with PstI (R0106L) and MspI (R3140L; both from New England Biolabs). Barcodes were ligated with T4 DNA ligase (New England Biolabs

(6) Multiomics Analysis of Skeletal Muscle Identifies Dysregulation of Hypoxia‐Induced Genes in Peripheral Artery DiseaseJournal of the American Heart AssociationOctober 7, 2025Ahmed Ismaeel, Emma Fletcher, Evlampia Papoutsi, Nicholas T. Thomas, William T. Bohannon, Iraklis I. Pipinos, Yuan Wen, Dimitrios Miserlis, Panagiotis Koutakisgenomic DNA (≈100 ng) was digested with 30 units of MspI (New England Biolabs, Ipswich, MA; Catalog No. R0106S). Fragments were ligated to preannealed adapters containing 5′‐methyl‐cytosine instead of cytosine according

(7) Multiomics Analysis of Skeletal Muscle Identifies Dysregulation of Hypoxia‐Induced Genes in Peripheral Artery DiseaseJ Am Heart AssocSeptember 30, 2025Ahmed Ismaeel, Emma Fletcher, Evlampia Papoutsi, Nicholas T. Thomas, William T. Bohannon, Iraklis I. Pipinos, Yuan Wen, Dimitrios Miserlis, Panagiotis Koutakisgenomic DNA (≈100 ng) was digested with 30 units of MspI (New England Biolabs, Ipswich, MA; Catalog No. R0106S). Fragments were ligated to preannealed adapters containing 5′‐methyl‐cytosine instead of cytosine according

(8) Resolution of Lipopolysaccharide-Induced Inflammation Followed by DNA Hypomethylation and Increased Tetrahydrobiopterin Biosynthesis in Mouse HippocampusBrain SciAugust 18, 2025Jennyffer Souza, Debora da Luz Scheffer, Alexandre Francisco Solano, Samantha Veloso, Luisa Cruz, Rodrigo Foganholi-Silva, Alexandra Latinihydroxymethylation of DNA. The samples were then digested with the restriction enzymes MspI or HpaII (R0106L, New England BioLabs,, Ipswich, MA, USA). Following DNA treatment, qPCR reactions were performed in

(9) CpG island demethylation and recruitment of SP1 to the promoter region regulates human thymic stromal lymphopoietin expressionEpigeneticsJuly 14, 2025Krishna Priya Ganti, Milan Surjitfollowing manufacturer’s instructions. Isolated DNA was subject to HpaII (R0171S, NEB, MA, USA) and MspI (R0106S, NEB, MA, USA) restriction digestion, followed by PCR amplification of the CpG2 and CpG3 islands. Primer

(10) Pain Hypersensitivity and Increased Urinary Tetrahydrobiopterin Levels in Mice Submitted to High-Fat DietBrain SciJune 16, 2025Tuany Eichwald, Débora da Luz Scheffer, Leonardo Barros, Alexandre Solano, Vivian De Souza Menegassi, Ananda Christina Staats Pires, Camila Sartor Spivakoski, Rodrigo A. Da Silva, Joana M. Gaspar, Marcelo Fernando Ronsoni, et al.phenol/chloroform/isoamyl alcohol method and subsequently digested with the restriction enzymes MspI or HpaII (R0106L, New England BioLabs, Ipswich, MA, USA) [38]. Following DNA treatment, qPCR reactions were performed

(11) Differentiation of European yellow rust subraces within the 'Warrior(-)' genetic groupPLoS OneMay 23, 2025Claudia Breitkreuz, Philipp Schulz, Anne-Kristin Schmitt, Kerstin Flath, Tiphaine Vidal, Amelia Hubbard, Kostya Kanyuka, Andreas Stahl, Jens Keilwagen, Dragan Perovicconcentrations were adjusted to 20 ng/µL and digested overnight at 37°C with the restriction enzymes MspI (NEB #R0106S; New England Biolabs GmbH, Frankfurt, Germany) and PstI (NEB #R3140) (Mastermix details in Table S3).

(12) Dnmt3a overexpression disrupts skeletal muscle homeostasis, promotes an aging-like phenotype, and reduces metabolic elasticityiScienceApril 18, 2025Mamoru Oyabu, Yuto Ohira, Mariko Fujita, Kiyoshi Yoshioka, Runa Kawaguchi, Atsushi Kubo, Yukino Hatazawa, Hinako Yukitoshi, Huascar Pedro Ortuste Quiroga, Naoki Horii, et al.Mix,Toyobo,Cat# QPX-201 HpaII,New England Biolabs Japan,Cat# R0171M MspI,New England Biolabs Japan,Cat# R0106S Ecoli ligation kit,Takara,Cat# 2160A Gene Taq,Nippongene,Cat# 318-02871 MinElute PCR Purification

(13) Fishing for Florida Bass in West Virginia: Genomic Evaluation of Florida Bass Presence and Establishing Baselines of Genetic Structure and Diversity for Native Largemouth BassBiology (Basel)April 9, 2025Andrew Johnson, Katherine Zipfel, Dustin Smith, Amy WelshIpswich, MA, USA), and 0.4 μL of PstI and MspI restriction enzymes (New England Biolabs #R3140L and #R0106L, respectively) and run with thermocycler conditions set to 37 °C for 120 min. and 80 °C for 20 min.

(14) The Distribution of Sport Performance Gene Variations Through COVID-19 Disease SeverityDiagnostics (Basel)March 12, 2025Guven Yenmis, Ilayda Kallenci, Mehmet Dokur, Suna Koc, Sila Basak Yalinkilic, Evren Atak, Mahmut Demirbilek, Hulya ArkanPPRGC1A rs8192678 and ACTN3 rs1815739 polymorphisms. One unit of the Mspl restriction enzyme (Catalog #R0106S, New England Biolabs, Ipswich, MA, USA) was used to digest the PPRGC1A PCR product. The PCR product–enzyme

(15) Cell-free epigenomes enhanced fragmentomics-based model for early detection of lung cancerClin Transl MedFebruary 5, 2025Yadong Wang, Qiang Guo, Zhicheng Huang, Liyang Song, Fei Zhao, Tiantian Gu, Zhe Feng, Haibo Wang, Bowen Li, Daoyun Wang, et al.illumina 150 PE program. For cfRRBS, 10 ng purified cfDNA was cleaved by MspI restriction enzyme (NEB, R0106L) at CCGG sites. Bisulphite conversion of the adapter‐ligated product was carried out with EZ DNA Methylation‐Lightning

(16) Coat colour in marsupials: genetic variants at the ASIP locus determine grey and black fur of the brushtail possumRoyal Society Open ScienceJuly 31, 2024Donna M. Bond, Andrew Veale, Alana Alexander, Timothy A. Horefollowing modifications: briefly, DNA was digested with PstI and MspI restriction enzymes (R0140L and R0106L: New England Biolabs, Ipswich, MA, USA)—these enzymes were chosen based on Agilent 2100 Bioanalyser

(17) miR-34a is a tumor suppressor in zebrafish and its expression levels impact metabolism, hematopoiesis and DNA damagePLOS GeneticsMay 28, 2024Sergey V. Prykhozhij, Kevin Ban, Zane L. Brown, Kim Kobar, Gabriel Wajnberg, Charlotte Fuller, Simi Chacko, Jacynthe Lacroix, Nicolas Crapoulet, Craig Midgen, et al.respectively. Detection of the R217H allele was done by R217_SA PCR as just described followed by MspI (NEB, R0106S) digestion [34], where the lack of digestion indicates a wild-type genotype and near-complete digestion

(18) Activity of zebrafish THAP9 transposase and zebrafish P element-like transposonsbioRxivMarch 27, 2024Nitzan Kutnowski, George E. Ghanim, Yeon Lee, Donald C. Rio,New England Biolabs,R0539S BsaI,New England Biolabs,R3535 EcoRI,New England Biolabs,R0101S MspI,New England Biolabs,R0106S NheI,New England Biolabs,R3131S NotI,New England Biolabs,R0189S PstI,New England Biolabs,R0140S SalI,New England Biolabs

(19) Inferring phylogenetic structure, taxa hybridization, and divergence times within rock voles of subgenus Aschizomys (Cricetidae: Alticola) using quaddRAD sequencing and a cytb datasetEcol EvolDecember 13, 2023Ivan A. Dvoyashov, Semyon Yu. Bodrov, Nikolai V. Mamaev, Elena S. Glagoleva, Natalia I. Abramson(cat. # R0140S, New England Biolabs) was added to 600 ng of purified DNA, 0.4 μL of MspI (U/μL) (cat. # R0106S, New England Biolabs), 0.6 μL of T4 ligase (400 U/μL) (cat. # M0202S, New England Biolabs), 6 μL of

(20) Protocol for generating high-quality genome-scale DNA methylation sequencing data from human cancer biospecimensSTAR ProtocolsDecember 1, 2023Euan J. Rodger, Peter A. Stockwell, Suzan Almomani, Michael R. Eccles, Aniruddha Chatterjee"PBS, pH 7.4",Thermo Fisher Scientific,Cat#: 10010023 MspI restriction endonuclease (20 U/μL),NEB,Cat#: R0106S NuSieve GTG agarose,Lonza,Cat#: 50081 KAPA HiFi HotStart Uracil+ ReadyMix,Roche,Cat#: KK2801 Quick-Load

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MspI

MspI from New England Biolabs

Sequence

New England Biolabs

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MspI from New England Biolabs

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