MspI from New England Biolabs

Product Specs
ItemMspI
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0106T
Quantity5000 units
Concentration100,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/CGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencingNucleic Acids ResMay 14, 2026Jingru Yu, Lauren S Ahmann, Yvette Y Yao, Angus Toland, Alicia Snowden, Chandler Ho, Netanel Loyfer, Tommy Kaplan, Hannes Vogel, Linlin Wang, et al.to quench the phosphatase. This was followed by restriction enzyme cutting using 10 units of MspI (R0106T, NEB), followed by a 30-min incubation. Next, an end repair was performed using 1 µL of Klenow master

(2) Adapting clinical chemistry plasma as a source for liquid biopsiesElifeMay 1, 2026Spencer C Ding, Jingru Yu, Tiepeng Liao, Lauren Ahmann, Yvette Yao, Chandler Ho, Linlin Wang, Benjamin A Pinsky, Wei GuBriefly, extracted cfDNA molecules were adapter-ligated, dephosphorylated, digested with MspI (NEB, #R0106T), adapter-ligated a second time with custom adapters, subjected to NEBNext Enzymatic Methyl-seq Conversion Module

(3) Adapting Clinical Chemistry Plasma as a Source for Liquid BiopsieseLifeMay 1, 2026Spencer C Ding, Jingru Yu, Tiepeng Liao, Lauren S Ahmann, Yvette Y Yao, Chandler Ho, Linlin Wang, Benjamin A Pinsky, Wei GuBriefly, extracted cfDNA molecules were adapter-ligated, dephosphorylated, digested with MspI (NEB, #R0106T), adapter-ligated a second time with custom adapters, subjected to NEBNext Enzymatic Methyl-seq Conversion Module

(4) Adapting Clinical Chemistry Plasma as a Source for Liquid BiopsiesmedRxivFebruary 27, 2026Spencer C. Ding, Jingru Yu, Tiepeng Liao, Lauren S. Ahmann, Yvette Y. Yao, Chandler Ho, Linlin Wang, Benjamin A. Pinsky, Wei GuBriefly, extracted cfDNA molecules were adapter-ligated, dephosphorylated, digested with MspI (NEB, #R0106T), adapter-ligated a second time with custom adapters, subjected to NEBNext Enzymatic Methylseq Conversion

(5) Rat models of lactose intolerance: sexually dimorphic comorbidities with neuropsychiatric disorders modulated by tryptophan hydroxylase and the dopaminergic systemScientific ReportsNovember 26, 2025Marcella C. Galvão, Nathalia A. Galvão, Vanessa S. A. da Silva, Tifany V. Rocha, Daniel N. L. Santos, Liebert B. Carvalho, Rodrigo A. F. da Silva, Thiago Berti KirstengDNA previously treated with endonucleases purchased from New England Biolabs, T4-Bgt (M0357S), MspI (R0106T) and HpaII (R0171L)76. Primer sequences were designed in regulatory regions with CpG islands within

(6) Association of Apolipoprotein C-III Gene Polymorphisms (rs2854116 and rs2854117) with Susceptibility to Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in a Turkish PopulationMedicina (Kaunas)August 18, 2025Damla Karaagac, Suat Morkuzu, Naci Senkal, Ersel Bilgin, Yasemin Oyacı, Tufan Tükek, Sacide Pehlivan, Alpay Medetalibeyoglurs2854116 variant, and MspI enzyme was used for the rs2854117 variant (New England Biolabs, cat no: R0109S, R0106T, respectively) (New England Biolabs, Ipswich, MA, USA). Restriction products were run on 3.5% agarose

(7) MeCP2 binds to methylated DNA independently of phase separation and heterochromatin organisationNature CommunicationsMay 8, 2024Raphaël Pantier, Megan Brown, Sicheng Han, Katie Paton, Stephen Meek, Thomas Montavon, Nicholas Shukeir, Toni McHugh, David A. Kelly, Tino Hochepied, et al.digested with methylation-sensitive (HpaII, NEB cat. R0171M) or methylation-insensitive (MspI, NEB cat. R0106M) restriction enzymes recognising the same ‘CCGG’ sequence within satellite I DNA. For detection of mouse

(8) Nonlinear DNA methylation trajectories in aging male miceNature CommunicationsApril 9, 2024Maja Olecka, Alena van Bömmel, Lena Best, Madlen Haase, Silke Foerste, Konstantin Riege, Thomas Dost, Stefano Flor, Otto W. Witte, Sören Franzenburg, et al.protocol. In brief, 200 ng of DNA was fragmented via a 5 h MspI-digest (New England Biolabs, Cat.no: R0106M), followed by TruSeq adapter ligation (Illumina, Cat.no: 20020595) and subsequent bisulfite conversion

(9) Regulatory architecture of housekeeping genes is driven by promoter assembliesCell reportsMay 30, 2023Dejosez M, Dall'Agnese A, Ramamoorthy M, Platt J, Yin X, Hogan M, Brosh R, Weintraub AS, Hnisz D, Abraham BJ, et al.15 min at 37°C. 25 μL of CutSmart buffer (25 μL; 10x, NEB, B7204S) and 2 μL of MspI (100 U/μl; NEB, R0106M) were added. After 4 and 8 h of incubation at 37°C, additional 2 μL of MspI were added for a total of

(10) Adverse Maternal Environments Perturb Hepatic DNA Methylome and Transcriptome Prior to the Adult-Onset Non-Alcoholic Fatty Liver Disease in Mouse OffspringNutrientsApril 30, 2023Qi Fu, Warren A. Cheung, Amber V. Majnik, Xingrao Ke, Tomi Pastinen, Robert H. Laneprepared using 250 ng of genomic DNA, digested with Msp1 (New England Biolabs (NEB), Ipswich, MA, USA, R0106M), and purified using the QIAquick Nucleotide Removal Kit (Qiagen, 28004). End-repair A tailing was performed

(11) KrasG12D induces changes in chromatin territories that differentially impact early nuclear reprogramming in pancreatic cellsGenome BiologyDecember 1, 2021Angela J. Mathison et al.preparation, and sequencing. Briefly, DNA (250 ng) was digested with Msp1 (New England Biolabs (NEB), R0106M) and purified using Qiaquick Nucleotide Removal Kit (Qiagen, 28004). End-repair A tailing was performed

(12) Rapid adaptation through genomic and epigenomic responses following translocations in an endangered salmonidEvolutionary ApplicationsOctober 1, 2021Marco Crotti, Elizabeth Yohannes, Ian J. Winfield, Alex A. Lyle, Colin E. Adams, Kathryn R. Elmer

(13) Extended-representation bisulfite sequencing of gene regulatory elements in multiplexed samples and single cellsNature BiotechnologySeptember 1, 2021Sarah J. Shareef, Samantha M. Bevill, Ayush T. Raman, Martin J. Aryee, Peter van Galen, Volker Hovestadt, Bradley E. Bernsteinpurified DNA or lysis buffer containing sorted cells. The final reaction contains 10 U MspI enzyme (NEB R0106M), 800 U T4 DNA ligase (NEB M0202M), 1.5 mM ATP (NEB P0756L), 10 nM annealed barcoded adapter, and 1x

(14) Effect of concentration and duration of particulate matter exposure on the transcriptome and DNA methylome of bronchial epithelial cellsEnvironmental EpigeneticsFebruary 28, 2021Steven K Huang, Priya Tripathi, Lada A Koneva, Raymond G Cavalcante, Nathan Craig, Anne M Scruggs, Maureen A Sartor, Furong Deng, Yahong Chenrepaired, A-tailed, and ligated (all using reagents from New England Biolabs, Ipswich, MA, catalog #s: R0106M, M0203L, M0210L, M0212L) to pair-end methylated adapters from Illumina. DNA was then resolved on agarose

(15) Polyglutamine-expanded ataxin3 alter specific gene expressions through changing DNA methylation status in SCA3/MJDAging (Albany NY)February 15, 2021Dongxue Ding, Chunrong Wang, Zhao Chen, Kun Xia, Beisha Tang, Rong Qiu, Hong JiangBGI-Shenzhen (Shenzhen, China). First, 3 μg of total genomic DNA from each sample was digested with MspI (NEB, R0106T), and then, DNA end repair, single A nucleotide addition, and multiplexed adapter ligation were performed.

(16) Alteration of the brain methylation landscape following postnatal inflammatory injury in rat pupsThe FASEB JournalJanuary 1, 2020Wyston C. Pierre, Lisa‐Marie Legault, Irene Londono, Serge McGraw, Gregory A. LodygenskyBriefly, 600 ng of genomic DNA was digested over night with non‐methylation specific Msp1 enzyme (NEB # R0106M). Ligation of methylated adaptors (NEB # E7535L) was done on the digested DNA with T4 ligase enzyme

(17) Alteration of the brain methylation landscape following postnatal inflammatory injury in rat pupsFASEB JNovember 22, 2019Wyston C. Pierre, Lisa‐Marie Legault, Irene Londono, Serge McGraw, Gregory A. LodygenskyBriefly, 600 ng of genomic DNA was digested over night with non‐methylation specific Msp1 enzyme (NEB # R0106M). Ligation of methylated adaptors (NEB # E7535L) was done on the digested DNA with T4 ligase enzyme

(18) A Unique Epigenomic Landscape Defines Human ErythropoiesisCell reportsSeptember 10, 2019Schulz VP, Yan H, Lezon-Geyda K, An X, Hale J, Hillyer CD, Mohandas N, Gallagher PG.04434 MethoCult H4330,StemCell Technologies,Cat# 04330 MspI restriction enzyme,New England Biolabs,Cat# R0106T T4 DNA polymerase,New England Biolabs,Cat# M0203S T4 DNA ligase reaction buffer,New England Biolabs,Cat#

(19) Rapid Multiplexed Reduced Representation Bisulfite Sequencing Library Prep (rRRBS)BIO-PROTOCOLFebruary 20, 2019Lisa-Marie Legault, Donovan Chan, Serge McGrawλ phage DNA (Promega, catalog number: D1521) 7. MspI 100 U/μl (New England Biolabs, catalog number: R0106M) 8. Klenow fragment 5,000 U/ml (New England Biolabs, catalog number: M0212L) 9. dNTP set (4 x 100 mM)

(20) Identifying DNA methylation biomarkers for non-endoscopic detection of Barrett’s esophagusScience Translational MedicineJanuary 17, 2018Helen R. Moinova et al.isolated from frozen esophageal biopsies or cell lines was digested with MspI (New England Biolabs # R0106T). End repair, A-tailing, and adapter ligation were carried out using the NEXTFlex Bisulfite-sequencing

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MspI

MspI from New England Biolabs

Description

Sequence

New England Biolabs

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MspI from New England Biolabs

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