RPMI-7951 from ATCC

Product Specs
ItemRPMI-7951
CompanyATCC
Price $1,995.00Supplier Page View Company Product Page
Catalog NumberHTB-66
TypeCell Biology
SpeciesHomo sapiens
TissueSkin
DiseaseMalignant Melanoma
Cell LineRPMI-7951
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Skin
Age18 years

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) ATR promotes mTORC1 activity via de novo cholesterol synthesisEMBO ReportsJune 13, 2025Naveen Kumar Tangudu, Alexandra N Grumet, Richard Fang, Raquel Buj, Aidan R Cole, Apoorva Uboveja, Amandine Amalric, Baixue Yang, Zhentai Huang, Cassandra Happe, et al.number Experimental models,Experimental models,Experimental models SKMEL28,ATCC,Cat # HTB-72 RPMI-7951,ATCC,Cat # HTB-66 HeLa,ATCC,Cat # CCL-2 HEK293-FT,ATCC,Cat # CRL-3249 HEK293,ATCC,Cat # CRL-1573 MEFs,Provided by

(2) Let-7a microRNA modulates caspase-3-dependent apoptosis in melanoma cells treated with dabrafenib and trametinib combinationIrish Journal of Medical Science (1971 -)March 10, 2025Murat Keser, Harika Atmacaapoptosis. Materials and methods Cell culture and reagents A375 (ATCC# CRL-1619) and RPMI-7951 (ATCC# HTB-66) human melanoma cells were obtained from American Type Culture Collection (ATCC). RPMI-7951 cells were

(3) MIRO2 promotes cancer invasion and metastasis via MYO9B suppression of RhoA activityCell ReportsJanuary 28, 2025Dillon P. Boulton, Connor J. Hughes, Valentina Vaira, Alessandro Del Gobbo, Alessandro Palleschi, Marco Locatelli, Etienne Danis, Masoom Raza, Andrew J. Neumann, Stephen Connor Purdy, et al.DU145, ATCC, catalog# HTB-81; and PC3, ATCC, catalog# CRL-1435), melanoma (RPMI-7951, ATCC, catalog# HTB-66; and SK-MEL-28, ATCC, catalog# HTB-72), and pancreatic cancer (MIA PaCa-2, ATCC, catalog# CRL-1420;

(4) The Lipid Droplet Protein DHRS3 Is a Regulator of Melanoma Cell StatePigment Cell & Melanoma ResearchJanuary 1, 2025Eleanor Johns, Yilun Ma, Pakavarin Louphrasitthiphol, Christopher Peralta, Miranda V. Hunter, Jeremy H. Raymond, Henrik Molina, Colin R. Goding, Richard M. Whitefrom Colin Goding (University of Oxford),N/A HS294T,ATCC,"HTB-140, (RRID:CVCL_0331)" RPMI-7951,ATCC,"HTB-66, (RRID:CVCL_1666)" IGR37 iß-Catenin,"Generated and validated as described in Louphrasitthiphol, Chauhan

(5) Inverse correlation between TP53 gene status and PD-L1 protein levels in a melanoma cell model depends on an IRF1/SOX10 regulatory axisCellular & Molecular Biology LettersSeptember 5, 2024Lucia Martinkova, Pavlina Zatloukalova, Martina Kucerikova, Nela Friedlova, Zuzana Tylichova, Filip Zavadil-Kokas, Ted Robert Hupp, Philip John Coates, Borivoj Vojtesek[25]), SK-MEL2 (ATCC HTB-68), SK-MEL-28 (ATCC HTB-72), SK-MEL-5 (ATTC HTB-70), and RPMI-7951 (ATTC HTB-66) were grown in DMEM. HT-144 (ATCC HTB-63), G-361 (ATCC CRL-1424), MALME-3 M (ATCC HTB-64), and SK-MEL-3

(6) Functional analysis of recurrent CDC20 promoter variants in human melanomaCommunications BiologyNovember 29, 2023Paula M. Godoy, Abimbola Oyedeji, Jacqueline L. Mudd, Vasilios A. Morikis, Anna P. Zarov, Gregory D. Longmore, Ryan C. Fields, Charles K. KaufmancBioPortal.org and plotted with ggplot2. Cell culture We obtained A375 (CRL-1619) and RPMI-7951 (HTB-66) cells from ATCC. SK-MEL-2, LOX-IMVI, SK-MEL-28, SK-MEL-5, UACC-62 cells were obtained directly from

(7) The Combined Metabolically Oriented Effect of Fucoidan from the Brown Alga Saccharina cichorioides and Its Carboxymethylated Derivative with 2-Deoxy-D-Glucose on Human Melanoma CellsInternational Journal of Molecular SciencesJuly 27, 2023Olesya S. Malyarenko, Roza V. Usoltseva, Artem S. Silchenko, Anastasiya O. Zueva, Svetlana P. ErmakovaConditions The cells of the human melanoma cell lines SK-MEL-28 (ATCC® no. HTB-72™), RPMI-7951 (ATCC® no. HTB-66™), and SK-MEL-5 (ATCC® no. HTB-70™) were purchased from the American Type Culture Collection (Manassas,

(8) Overexpression of Krüppel-Like Factor 9 Enhances the Antitumor Properties of Paclitaxel in Malignant Melanoma-Derived Cell LinesPharmaceuticals (Basel)April 6, 2023Mohammed O. Altonsy, George X. Song-Zhao, Mahmoud M. Mostafa, Paule Régine Mydlarskigrowth kit (catalog no. psc-200-041, ATCC). Human malignant melanoma cell lines, RPMI-7951 (catalog no. htb-66; ATCC), and A375 cells (catalog no. crl-1619; ATCC) were cultured in DMEM medium (catalog no. 12-604f;

(9) Effect of Phlorotannins from Brown Algae Costaria costata on α-N-Acetylgalactosaminidase Produced by Duodenal Adenocarcinoma and Melanoma CellsMarine drugsDecember 30, 2022Bakunina I, Imbs T, Likhatskaya G, Grigorchuk V, Zueva A, Malyarenko O, Ermakova S.direct inhibitory effects on the cancer cell-associated α-NaGalase of the lines RPMI-7951 (ATCC #no. HTB-66™), MDA-MB-231 (ATCC#no.HTB-26™), DLD-1 (ATCC#no.CCL-221), HT-29 (ATCC#no.HTB-38™), HCT-116 (ATCC #no.CCL-247),

(10) MEK inhibition enhances presentation of targetable MHC-I tumor antigens in mutant melanomasProceedings of the National Academy of Sciences of the United States of AmericaDecember 6, 2022Stopfer LE, Rettko NJ, Leddy O, Mesfin JM, Brown E, Winski S, Bryson B, Wells JA, White FM.SKMEL28, A375, RPMI-7951, and T2 cell lines were obtained from ATCC [ATCC HTB-70, ATCC HTB-72, CRL1619, HTB-66, and CRL-1992, respectively] and maintained in Dulbecco's Modified Eagle Medium (DMEM) (Corning). IPC298

(11) Loss of NECTIN1 triggers melanoma dissemination upon local IGF1 depletionNature geneticsDecember 1, 2022Ablain J, Al Mahi A, Rothschild H, Prasad M, Aires S, Yang S, Dokukin ME, Xu S, Dang M, Sokolov I, et al.A375, SKMEL2, COLO679, and G361 cell lines were purchased from commercial sources (ATCC CRL-3216, ATCC HTB-66, Creative Bioarray CSC-C6421J, ATCC CRL-1619, ATCC HTB-68, Sigma 87061210, and ATCC CRL-1424, respectively)

(12) The C30-Modulation of Betulinic Acid Using 1,2,4-Triazole: A Promising Strategy for Increasing Its Antimelanoma Cytotoxic PotentialMoleculesNovember 12, 2022Gabriela Nistor, Marius Mioc, Alexandra Mioc, Mihaela Balan-Porcarasu, Roxana Racoviceanu, Alexandra Prodea, Andreea Milan, Roxana Ghiulai, Alexandra Semenescu, Cristina Dehelean, et al.CLS Cell Lines Service GmbH (Eppelheim, Germany), and RPMI-7951—human malignant melanoma cells (ATCC® HTB-66™) were procured from American Type Culture Collection (ATCC, Lomianki, Poland). DMEM, containing 10%

(13) Endogenous DOPA inhibits melanoma through suppression of CHRM1 signalingScience AdvancesSeptember 2, 2022Miriam Doepner, Inyoung Lee, Christopher A. Natale, Roderick Brathwaite, Swati Venkat, Sung Hoon Kim, Yiliang Wei, Christopher R. Vakoc, Brian C. Capell, John A. Katzenellenbogen, et al.1% penicillin-streptomycin. RPMI-7951 and SK-MEL-24 cells were purchased from ATCC (RPMI-7951 ATCC HTB-66; ATCC HTB-71) and cultured in Eagle’s minimum essential medium with 15% FBS and 1% penicillin-streptomycin.

(14) Systematic discovery of mutation-directed neo-protein-protein interactions in cancerCellMay 26, 2022Mo X, Niu Q, Ivanov AA, Tsang YH, Tang C, Shu C, Li Q, Qian K, Wahafu A, Doyle SP, et al.RRID:CVCL_0527 LOXIMVI cell line,Millipore,Cat# SCC201; RRID:CVCL_1381 RPMI-7951 cell line,ATCC,Cat# HTB-66; RRID:CVCL_1666 SK-MEL-28 cell line,ATCC,Cat# HTB-72; RRID:CVCL_0526 UACC257 (U257) cell line,NCI-DTP,Cat#

(15) Weight-bearing activity impairs nuclear membrane and genome integrity via YAP activation in plantar melanomaNature CommunicationsApril 25, 2022Jimyung Seo, HyunSeok Kim, Kyoung Il Min, Changgon Kim, Yongsoo Kwon, Zhenlong Zheng, Yusung Kim, Hyung-Soon Park, Young Seok Ju, Mi Ryung Roh, et al.(Welgene) for RPE1 (ATCC: CRL-4000™); MEM (Welgene) for SKMEL28 (ATCC: HTB-72™) and RPMI-7951 (ATCC: HTB-66™)] supplemented with 10% FBS and 1% penicillin/streptomycin. Stable clonal cell lines were generated

(16) Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant MelanomaSSRN Electronic JournalApril 1, 2022Mayinuer Maitituoheti et al.HMEL-BRAFV600E Gift from Dr. David Fisher’s laboratory N/A Human: A375 ATCC CRL-1619 Human: RPMI-7951 ATCC HTB-66 Human: WM115 ATCC CRL-1675 Human: WM266-4 ATCC CRL-1676 Human: SKMEL-24 ATCC HTB-71 Human:

(17) Selective Targeting of Protein Kinase C (PKC)-θ Nuclear Translocation Reduces Mesenchymal Gene Signatures and Reinvigorates Dysfunctional CD8+ T Cells in Immunotherapy-Resistant and Metastatic CancersCancers (Basel)March 21, 2022Jenny Dunn et al.(ATCC HTB-26), MCF-7 (ATCC HTB-22), B16-F10 (ATCC CRL-6475), 4T1 (ATCC CRL-2539), and RPMI-7951 (ATCC HTB-66) cells were cultured in high-glucose DMEM supplemented with 2 mM L-glutamine, 1 × penicillin-streptomycin-neomycin

(18) In Vitro Anticancer and Cancer-Preventive Activity of New Triterpene Glycosides from the Far Eastern Starfish Solaster pacificusMarine DrugsMarch 20, 2022Timofey V. Malyarenko, Olesya S. Malyarenko, Alla A. Kicha, Anatoly I. Kalinovsky, Pavel S. Dmitrenok, Natalia V. Ivanchinamelanoma cells SK-MEL-2 (ATCC® no. HTB-68™), SK-MEL-28 (ATCC® no. HTB-72™), and RPMI-7951 (ATCC® no. HTB-66™). JB6 Cl41 cells were cultured in Minimal Essential Medium (MEM) and SK-MEL-2, SK-MEL-28, and RPMI-7951

(19) Profiling of diverse tumor types establishes the broad utility of VHL-based ProTaCs and triages candidate ubiquitin ligasesiScienceMarch 18, 2022Xin Luo, Ivonne Archibeque, Ken Dellamaggiore, Kate Smither, Oliver Homann, James Russell Lipford, Dane MohlRRID:CVCL_1057 A2058,ATCC,CRL-11147; RRID:CVCL_1059 A-375,ATCC,CRL-1619; RRID:CVCL_0132 RPMI 7951,ATCC,HTB-66; RRID:CVCL_1666 SK-MEL-28,ATCC,HTB-72; RRID:CVCL_0526 AGS,ATCC,CRL-1739; RRID:CVCL_0139 UM UC-3,ATCC,CRL-1749;

(20) Macrophage-Mediated Melanoma Reduction after HP-NAP Treatment in a Zebrafish Xenograft ModelInternational Journal of Molecular SciencesJanuary 31, 2022Gaia Codolo, Nicola Facchinello, Nicole Papa, Ambra Bertocco, Sara Coletta, Clara Benna, Luigi Dall’Olmo, Simone Mocellin, Natascia Tiso, Marina de BernardLines Human melanoma M121224 (University Research Priority Program–Melanoma Biobank Zurich), RPMI-7951 (HTB-66, ATCC, Manassas, VA, USA) and A375 (CRL-1619, ATCC) cell lines were used for the experiments. M121224

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RPMI-7951 from ATCC

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