hTERT RPE-1 from ATCC

Product Specs
ItemhTERT RPE-1
CompanyATCC
Price $8,500.00Supplier Page View Company Product Page
Catalog NumberCRL-4000
TypeCell Biology
SpeciesHomo sapiens
TissuePigmented epithelium:Retina, Eye
Cell LinehTERT RPE-1
Validated Species ReactivityHuman (Hu)
Cell Typeepithelial cell
DiseaseNormal
Tissue (standardized)Retina

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(21) CD8 T lymphocytes deploy embryonic cell cycle control mechanisms for rapid cell proliferationEMBO ReportsJune 18, 2026David A Lewis, Ananya Kar, Alice Savage, Van Kelly, David Wright, Devin Tan, Mary Tozer, Christina Rollings, Doreen A Cantrell, Rose Zamoyska, et al.source,Identifier or catalog number Experimental models,Experimental models,Experimental models hTERT-RPE1,ATCC,CRL-4000 mESC E14Tg2a,https://www.sciencedirect.com/science/article/pii/S2211124725005108, NIH3T3,ATCC,CRL-1658 Recombinant

(22) Characterization of membrane structures regulating primary ciliogenesis by quantitative isotropic ultrastructure imagingNature CommunicationsJune 13, 2026Quanlong Lu, Huijie Zhao, Ziam Khan, Adam Harned, Erina Kamiya, Valentin Magidson, Abhi Senthilkumar, Avaneesh Kilnagar, Phuong Thi Bich Doan, Sumeth Perera, et al.obtained from Sigma and used according to manufacturer’s instructions. Cell lines Human hTERT-RPE1 (CRL-4000) and 293 T (CRL-3216) cell lines were obtained from ATCC. The human fibroblast cell line was obtained

(23) Contractile perinuclear actomyosin network promotes peripheral and polar chromosome interaction with the mitotic spindleElifeJune 11, 2026Nooshin Sheidaei, John K Eykelenboom, Zuojun Yue, Graeme Ball, Alexander JR Booth, Tomoyuki U TanakaCCL-247; male), Caco2 (ATCC HTB-37; male), and hTERT RPE1 (referred to in this manuscript as RPE1; ATCC CRL-4000; female) were all obtained from American Type Culture Collection. U2OS cdk1-as cells and derivative

(24) Proliferating toward sex: characterization of cell division of Toxoplasma gondii ’s pre-sexual stagesmBioJune 10, 2026Florencia Sena, Mohamed-Ali Hakimi, Maria E. FranciaVero cells (kidney of an African green monkey, ATCC CCL-81). hRPE (human retinal pigmented epithelial, ATCC CRL-4000) cells were used as host cells for all immunofluorescence assays. Parasites and host cells under sterile

(25) Nek1 defines a branch of centriolar microtubule length control parallel to CP110-Cep97Nature CommunicationsJune 9, 2026J. M. S. Streubel, O. R. Karasu, I. M. Munoz, A. Neuner, E. N. Numanoglu, T. J. Macartney, E. Schiebel, J. Rouse, G. Pereira2xFLAG. Cell culture and transfection Cells used in this study were hTERT-immortalized RPE1 (ATCC CRL-4000), ARPE-1935, ARPE-19 NEK1 KO and NEK1 KO stably expressing either NEK1 WT (also referred to as NEK1

(26) Covalent inhibitors of human papillomavirus type 16 E6 protein restore p53 function and suppress growth of HPV-driven tumors in vivoProceedings of the National Academy of SciencesJune 9, 2026Anne Rietz, Lokesh Kumari, Ankeeta Koirala, Stephane Pelletier, Zhijian Lu, Elliot J. AndrophyCell Culture SiHa (ATCC, HTB-35), C51S, CaSki (ATCC, CRL-1550), UM-SCC-47, UM-SCC-104, HeLa, C33a and RPE-1 (ATCC, CRL-4000) cells were cultured in Dulbecco’s modified Eagle Medium (DMEM) (Corning#10–013-CV), supplemented with

(27) Optimization of IS621 recombinase/bridge RNA-directed recombination for precise insertion of large DNA fragments in human cellsNature CommunicationsJune 6, 2026Junfan Guo, Ziguo Song, Wuke Wang, Shisheng Huang, Yu Zhang, Guanglei Li, Xingxu Huang, Xiaolong Wang, Jianghuai Liu, Lina Wuin Supplementary Data 4. Cell lines HEK293T (ATCC, CRL-3216), HCT116 (ATCC, CCL-247), RPE-1 (ATCC, CRL-4000), and Huh-7 (Cellosaurus, CVCL_0336) cells were cultured and passaged in Dulbecco’s Modified Eagle Medium

(28) PKMYT1 has an important role in the timing and fidelity of chromosome segregationEMBO ReportsJune 5, 2026Asma Belbelazi, Alexandra Hanna Hendry, Eleanor Wendy Trotter, Charlie Greenaway-Wells, Zoe Edwards, Keren Dawson, Jonathon Pines, Iain HaganSource,Identifier or Catalog Number Experimental models,Experimental models,Experimental models hTERT-RPE1,ATCC,CRL-4000 HeLa,ATCC,CCL-2 Recombinant DNA,Recombinant DNA,Recombinant DNA eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA,Addgene

(29) High-fold Homogeneous Expansion Microscopy Reveals Ultrastructural CentriolesACS NanoJune 2, 2026Wen-Qing Yang, Ting-Jui Ben Chang, Liang-Chen Pan, T. Tony YangThermo Fisher Scientific). Cell Culture Human retinal pigment epithelial cells (hTERT RPE-1, ATCC-CRL-4000) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 and HEPES (1:1; Gibco, Thermo Fisher

(30) Quantitative analysis of autophagic flux reveals radiation-induced activation of SQSTM1-mediated degradation of protein aggregates and ER-phagyJournal of Radiation ResearchMay 26, 2026Takahito Moriwaki, Tsuyoshi Masudamolecular targets for cancer radiation therapy. MATERIALS AND METHODS Cell culture hTERT/RPE-1 cells (CRL-4000) were purchased from ATCC. The cells were cultured in DMEM (high-glucose, GlutaMAX supplement and HEPES;

(31) CIP2A recruits SLX4-MUS81-XPF in mitosis and protects against replication stressEMBO ReportsMay 26, 2026Alice Meroni, Annica Pellizzari, Nathalie Varisco, Francesco Leone, Giada Greco, Andrea Hänel, Pascale Brasier-Lutz, Isabell Witzel, Alessandro A Sartori, Manuel StuckiFKBP12 (B6L),Dan Durocher,"Adam et al, 2021" DLD1 BRCA2 KO,ATCC,CVCL_HD57 hTERT RPE-1 (RPE-1),ATCC,CRL-4000 RPE-1 CIP2A KO,This lab,"De Marco Zompit et al, 2022" RPE-1 p53 KO,Dan Durocher,"Adam et al, 2021" RPE-1

(32) Genetically encoded manipulation of ATP/ADP ratio in human cells uncovers proteomic and physiological signatures of energy stressCell Chemical BiologyMay 21, 2026Alex E. Ekvik, Megan M. Kober, Denis V. Titovrequest. STAR★METHODS EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS hTERT-RPE1 cells hTERT-RPE1 cells (ATCC #CRL-4000), from retinal epithelium of a 1-year-old human female, were obtained from UC Berkeley Cell Culture

(33) GPC6 facilitates progression of SHH-subgroup medulloblastoma by enhancing Hedgehog secretion and signaling responsesJ Biomed ResMay 21, 2026Yue Wang, Qingyue Meng, Qin Zhu, Xinyi Zhang, Xinfa Wang, Junping He, Jing Cai, Xiaohong Pu, Zihe Ai, Qinya Li, et al.ONS-76 cells (RRID: CVCL_1624), maintained in RPMI-1640 medium (Wisent), hTERT-RPE1 cells (ATCC, Cat. #CRL-4000, RRID: CVCL_4388), grown in DMEM/F12 media (Wisent), MEF cells (ATCC, Cat. #CRL-2991, RRID: CVCL_L690),

(34) Dynamic regulation of origin firing factors links CDK activity to dormant origin activationNature CommunicationsMay 20, 2026Md Shahadat Hossain, Courtney G. Sansam, Kimberlie A. Wittig, Tyler D. Noble, Kevin A. Boyd, Christopher L. SansamhTERT-RPE-1 immortalized human retinal pigment epithelial cells were obtained from ATCC (CCL-247 and CRL-4000) and cultured in McCoy’s 5 A or Dulbecco’s Modified Eagle’s F12 Media (Corning), respectively, supplemented

(35) Genome instability triggers intercellular DNA transfer between human cellsCellMay 19, 2026Elizabeth G. Maurais, Alice Mazzagatti, Yu-Fen Lin, Maria Narozna, Qing Hu, Rashmi Dahiya, Derek Santiago-Ferrer, Conor P. Herlihy, Mary Krebs, Nikoleta Pateraki, et al.zeoR,This study,N/A HeLa S3 + H2B-mCherry,This study,N/A HeLa S3 + H2B-GFP,This study,N/A RPE-1,ATCC,CRL-4000 RPE-1 + dnTRF2,John Maciejowski,Maciejowski et al.57 RPE-1 + H2B-mCherry,This study,N/A RPE-1 + H2B-GFP,This

(36) Plasmodium falciparum PfMyoF contains a Rab-like tail domain and associates with perinuclear membrane trafficking proteinsCommunications BiologyMay 15, 2026Alexander J. Holmes, Philip Ilani, Christopher Batters, Alison Kemp, Yaw Aniweh, Lucas N. Amenga-Etego, Gordon A. Awandare, Julian C. Rayner, Folma Bussa pTM score of 0.58. Mammalian cell culture Human Retinal Pigment Epithelial (hTERT RPE-1, ATCC, CRL-4000) cells were maintained in Dulbecco’s Modified Eagle’s medium (D6546, Sigma Aldrich)—HAM’s F-12 Nutrient

(37) The Host Cell Factor Phosphatase‐2A Subunit PR130 Restricts Replication of Herpes Simplex Virus Type‐1Advanced ScienceMay 15, 2026Johannes Jungwirth, Christoph F. Jacob, Alexandra Nguyen, Mandy Beyer, Andreas O. Mieland, Mario Dejung, Jia‐Xuan Chen, Walburgis Brenner, Christina Ehrhardt, Andreas Henke, et al.HCT116 cells (RRID:CVCL_0291) in [49]. Human retinal pigment epithelial cells (hTERT RPE‐1) (RRID:CVCL_4388) (CRL‐4000, ATCC, Manassas, Virginia, USA; provided by Prof. T. Hofmann, Mainz, Germany) and MIA PaCa‐2 cells (RRID:CVCL_0428)

(38) Vitamin A Modulates AHR Signaling and Restricts Zika Virus Replication in Human Retinal Pigment Epithelial Cells: Insights from Molecular Modeling and Antiviral AssaysPathogensMay 12, 2026Agostina B. Marquez, Priscila A. Lanza Castronuovo, Cecilia L. Barbieri, Mayra A. Castañeda Cataña, Claudia S. Sepúlveda, Agustina Alaimo, D. Mariano A. Vera, Cybele C. Garcíaderived from human telomerase reverse transcriptase-immortalized retinal pigment epithelial cells (ATCC CRL-4000) were used. Both cell lines were cultured in Eagle’s minimal essential medium (MEM; Gibco, Thermo Fisher

(39) AAK1-mediated phosphorylation of PDLIM5 and Talin1 promotes focal adhesion disassembly to accelerate cell migrationNature CommunicationsMay 4, 2026Daniela Krocianova, Alexander D. Dagg, Rory A. Clayton, David Potesil, Veronika Fedorova, Adam Harmanec, Viktoria Benova, Veronika Bosakova, Jonathan G. G. Kaufman, Petra Martinkova, et al.dysregulated. Cell lines and cell culture All cell lines were obtained from ATCC: hTERT-RPE1 (ATCC CRL-4000)70, MDA-MB-231 (ATCC HTB-26), SH-SY5Y (ATCC CRL-2266), A-431 (ATCC CRL-1555), Saos-2 (ATCC HTB-85) and

(40) Bulky PP1 analogs exert cellular effects independently from analog-sensitive kinase inhibitionFrontiers in ChemistryApril 28, 2026Coralie Gicquel, Sabine Genicot, Arnaud Comte, Pierre ColasCell cultures We grew hTERT-RPE1 (telomerase-immortalized human retinal pigment epithelium cells; ATCC CRL-4000), HaCaT (immortalized human keratinocytes; AddexBio T0020001), and MDA-MB-231 (human breast adenocarcinoma

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hTERT RPE-1 from ATCC

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