Mouse Methylcellulose Complete Media from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Methylcellulose Complete Media
CompanyR&D Systems, a Bio-Techne Brand
Price $599.00Supplier Page View Company Product Page
Catalog NumberHSC007
SpeciesMouse
Quantity100 mL
TypeMedia
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 100 mL

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) HIV-1 Nef generates lasting innate immune memory in haematopoietic stem and progenitor cells in vivoEMBO ReportsJune 15, 2026Andrew J Fleetwood, Nigora Mukhamedova, Dragana Dragoljevic, Man K S Lee, Yangsong Xu, Malathi S I Dona, Ian Hsu, Camilla Bertuzzo Veiga, Fumihiko Takeuchi, Ben Crossett, et al.Density Gradient Medium,StemCell Tech,Cat#07820 Mouse Methylcellulose Complete Media,R&D Systems,Cat#HSC007 "Tetramethylrhodamine, Ethyl Ester, Perchlorate (TMRE)",Thermo Fisher Scientific,Cat#T669 MitoTracker™

(2) Dissecting infant leukemia developmental origins with a hemogenic gastruloid modeleLifeSeptember 11, 2025Denise Ragusa, Chun Wai Suen, Gabriel Torregrosa Cortes, Fabio Pastorino, Ayona Johns, Ylenia Cicirò, Liza Dijkhuis, Susanne van den Brink, Michele Cilli, Connor Byrne, et al.supplement,Gibco,Cat. #17504044,Medium Other,Mouse methylcellulose complete media,R&D systems,Cat. #hsc007,Medium Cell line (M. musculus),Kdr(Flk1)-GFP mouse embryonic stem cell line,"Alfonso Martinez Arias

(3) Dissecting infant leukemia developmental origins with a hemogenic gastruloid modeleLifeSeptember 11, 2025Ragusa D, Suen CW, Torregrosa Cortes G, Pastorino F, Johns A, Cicirò Y, Dijkhuis L, van den Brink S, Cilli M, Byrne C, et al.supplement,Gibco,Cat. #17504044,Medium Other,Mouse methylcellulose complete media,R&D systems,Cat. #hsc007,Medium Cell line (M. musculus),Kdr(Flk1)-GFP mouse embryonic stem cell line,"Alfonso Martinez Arias

(4) Loss of Bicra/Gltscr1 leads to a defect in fetal liver macrophages responsible for erythrocyte maturation in micebioRxiv : the preprint server for biologyOctober 19, 2024Sood S, Alpsoy A, Jiao G, Dhiman A, King CS, Conjelko G, Hallett J, Utturkar S, Hutchcroft J, Dykhuizen E.

(5) Piplartine eliminates CD34 + AML stem/progenitor cells by inducing oxidative stress and suppressing NF-κB signallingCell Death DiscovMarch 19, 2024Ana Carolina B. da C. Rodrigues, Suellen L. R. Silva, Ingrid R. S. B. Dias, Rafaela G. A. Costa, Maiara de S. Oliveira, Milena B. P. Soares, Rosane B. Dias, Ludmila F. Valverde, Clarissa A. G. Rocha, Emily M. Johnson, et al.[47] were plated at a density of 500 cells/plate in duplicate in mouse methylcellulose complete media HSC007 (R&D Systems). C57BL/6 mouse bone marrow cells were plated at a density of 50,000 cells/plate in the

(6) An iron rheostat controls hematopoietic stem cell fateCell Stem CellMarch 1, 2024Yun-Ruei Kao, Jiahao Chen, Rajni Kumari, Anita Ng, Aliona Zintiridou, Madhuri Tatiparthy, Yuhong Ma, Maria M. Aivalioti, Deeposree Moulik, Sriram Sundaravel, et al.LSK cells and their respective replicate wells were subjected to methylcellulose colony assay with HSC007 (R&D Systems) at 37°C, 5% CO2 for 1 week. Colony forming units were identified and scored using inverted

(7) Deregulated protein homeostasis constrains fetal hematopoietic stem cell pool expansion in Fanconi anemiaNature communicationsFebruary 29, 2024Kovuru N, Mochizuki-Kashio M, Menna T, Jeffrey G, Hong Y, Me Yoon Y, Zhang Z, Kurre P.using trypan blue stain and mixed with cytokine-supplemented mouse methylcellulose media (R&D Systems, HSC007). Triplicates were cultured in 3.5 cm dishes at 37 °C. After 7 days, colony numbers were scored under

(8) Streptavidin-drug conjugates streamline optimization of antibody-based conditioning for hematopoietic stem cell transplantationbioRxiv : the preprint server for biologyFebruary 12, 2024Yelamali AR, Chendamarai E, Ritchey JK, Rettig MP, DiPersio JF, Persaud SP.cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly. Finally, 1.1 mL of this methylcellulose suspension

(9) Complete miRNA-15/16 loss in mice promotes hematopoietic progenitor expansion and a myeloid-biased hyperproliferative stateProceedings of the National Academy of SciencesOctober 24, 2023Anita Ng, Francesca Lovat, Andrew J. Shih, Yuhong Ma, Yuri Pekarsky, Frank DiCaro, Lita Crichton, Esha Sharma, Xiao Jie Yan, Daqian Sun, et al.Assay. FACS-sorted CD150+CD48− HSC and CD150−CD48+ MPP cells were plated in Mouse Methylcellulose Complete Media (HSC007, R&D Systems, Minneapolis, MN) in the presence of Primocin (ANTPM1, Invitrogen, Waltham, MA). After

(10) Role of Cytokine-Inducible SH2 Domain-Containing (CISH) Protein in the Regulation of ErythropoiesisBiomoleculesOctober 12, 2023Maymand S, Lakkavaram AL, Naser W, Rasighaemi P, Dlugolenski D, Liongue C, Stambas J, de Koning-Ward TF, Ward AC.

(11) Preclinical Assessment of ADAM9-Responsive Mesoporous Silica Nanoparticles for the Treatment of Pancreatic CancerInternational journal of molecular sciencesJune 27, 2023Slapak EJ, El Mandili M, Brink MST, Kros A, Bijlsma MF, Spek CA.PBS and counted. A total of 90,000 cells were added to 3 mL mouse methylcellulose complete medium (#HSC007, R&D Systems, Minneapolis, MN, USA) containing varying concentrations of free paclitaxel, capped paclitaxel-loaded

(12) Replication stress increases mitochondrial metabolism and mitophagy in FANCD2 deficient fetal liver hematopoietic stem cellsFrontiers in OncologyMarch 7, 2023Makiko Mochizuki-Kashio, Noriko Otsuki, Kota Fujiki, Sherif Abdelhamd, Peter Kurre, Markus Grompe, Atsushi Iwama, Kayoko Saito, Ayako Nakamura-IshizuAria II or III (BD) and 500 cells are seeded onto 3ml of mouse methylcellulose media (R&D Systems, HSC007) with APH (50-100ng/ml) or imTOR of Rapamycin (Thermo PHZ1235, diluted with DMSO for 10mM and used as

(13) Effect of spermidine on radiation-induced long-term bone marrow cell injuryInternational immunopharmacologyJanuary 1, 2023Guan B, Li C, Yang Y, Lu Y, Sun Y, Su L, Shi G, Bai L, Liu J, Meng A.

(14) Cish knockout mice exhibit similar outcomes to malaria infection despite altered hematopoietic responsesFrontiers in microbiologyJanuary 1, 2023Lakkavaram AL, Maymand S, Naser W, Ward AC, de Koning-Ward TF.

(15) A glycan-based approach to cell characterization and isolation: Hematopoiesis as a paradigmJournal of Experimental MedicineNovember 7, 2022Richard T. Piszczatowski, Emily Schwenger, Sriram Sundaravel, Catarina M. Stein, Yang Liu, Pamela Stanley, Amit Verma, Deyou Zheng, Ronald D. Seidel, Steven C. Almo, et al.methylcellulose colony assays, FACS-purified c-kit+ (5,000 per well) bone marrow cells were plated in HSC007 methylcellulose (R&D Systems). For BFU-E assays, FACS-purified MEP (3,500 per well) or c-kit+ cells

(16) Influenza A virus infection instructs hematopoiesis to megakaryocyte-lineage outputCell reportsOctober 4, 2022Rommel MGE, Walz L, Fotopoulou F, Kohlscheen S, Schenk F, Miskey C, Botezatu L, Krebs Y, Voelker IM, Wittwer K, et al.

(17) RXRA DT448/9PP generates a dominant active variant capable of inducing maturation in acute myeloid leukemia cellsHaematologicaFebruary 1, 2022Orsola Di Martino, Margaret A. Ferris, Gayla Hadwiger, Soyi Sarkar, Anh Vu, MarÃa P. MenÃ©ndez-GutiÃ©rrez, Mercedes Ricote, John S. Welchproliferation analysis. Colony forming was evaluated in methylcellulose with complete cytokines (R&D Systems HSC007). 3,000 cells were plated in replicate in 1.1 mls and colonies assessed after 7 days. Cell proliferation

(18) Proof of Concept of the Radiosensitizing Effect of Gadolinium Oxide Nanoparticles in Cell Spheroids and a Tumor-Implanted Murine Model of ChondrosarcomaInternational journal of nanomedicineJanuary 1, 2022Aloy MT, Sidi Boumedine J, Deville A, Kryza D, Gauthier A, Brichart-Vernos D, Ollier G, La Padula V, Lux F, Tillement O, et al.

(19) Transcriptional responses of skeletal stem/progenitor cells to hindlimb unloading and recovery correlate with localized but not systemic multi-systems impactsNPJ microgravityNovember 26, 2021Booker CN, Haga CL, Boregowda SV, Strivelli J, Phinney DG.12-115F) with 2% FBS were added to 1.2 mL of thawed mouse methylcellulose with growth factors (R&D Systems HSC007) and thoroughly mixed by vortexing. The cell/methylcellulose mixture (1.1 mL) was plated into a 35 mm

(20) Chemotherapy-induced transposable elements activate MDA5 to enhance haematopoietic regenerationNature cell biologyJuly 1, 2021Clapes T, Polyzou A, Prater P, Sagar, Morales-Hernández A, Ferrarini MG, Kehrer N, Lefkopoulos S, Bergo V, Hummel B, et al.inhibitor, 4318, Tocris). Each well was transferred into 900 μl of Mouse Methylcellulose Complete Media (HSC007, R&D systems), and split into 3 separate wells of a 24-well plate. Colonies were counted after 7 d at

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