Chaps Cell Extract Buffer (10X) from Cell Signaling Technology

Product Specs
ItemChaps Cell Extract Buffer (10X)
CompanyCell Signaling Technology
Price $83.00 Inquire
Catalog Number9852S
Quantity1 Kit
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Loss of neuronal Imp contributes to seizure behavior through Syndecan function Neuronal Imp contributes to seizures via SyndecaneNeuroApril 24, 2025Paula R. Roy, Nichole Linkstop fixation, rinsed in PBS, and transferred to Chaps cell extract buffer (Cell Signaling Technology #9852) with Pierce phosphatase and protease inhibitors (Thermo Fisher Scientific #A32961) or Halt protease

(2) Exosomal TNF-α mediates voltage-gated Na+ channel 1.6 overexpression and contributes to brain tumor–induced neuronal hyperexcitabilityJournal of Clinical InvestigationSeptember 17, 2024Cesar Adolfo Sanchez Trivino, Renza Spelat, Federica Spada, Camilla D’Angelo, Ivana Manini, Irene Giulia Rolle, Tamara Ius, Pietro Parisse, Anna Menini, Daniela Cesselli, et al.cell culture medium, exosomes were resuspended in CHAPS Cell Extract Buffer (Cell Signaling Technology, 9852S) supplemented with protease inhibitor cocktail (Thermo Fisher Scientific, 78430), and 4× Laemmli buffer

(3) An N‐terminal and ankyrin repeat domain interactome of Shank3 identifies the protein complex with the splicing regulator Nono in miceGenes to CellsSeptember 1, 2024Sayaka Okuzono, Fumihiko Fujii, Daiki Setoyama, Ryoji Taira, Yohei Shinmyo, Hiroki Kato, Keiji Masuda, Kousuke Yonemoto, Satoshi Akamine, Yuki Matsushita, et al.Neuro2a cells transiently expressing HA‐Shank3 were washed in PBS and lysed in CHAPS lysis buffer (#9852, Cell Signaling Technology, Danvers, MA, USA) with protease inhibitor cocktail (Sigma‐Aldrich, St. Louis,

(4) The amino acid sensor GCN2 suppresses terminal oligopyrimidine (TOP) mRNA translation via La-related protein 1 (LARP1)The Journal of biological chemistrySeptember 1, 2022Farooq Z, Kusuma F, Burke P, Dufour CR, Lee D, Tabatabaei N, Toboz P, Radovani E, Greenblatt JF, Rehman J, et al.that expressed endogenously 3xFlag-tagged GCN1. Chaps cell extract buffer (Cell Signaling Technology #9852) in presence or absence of RNase A (Sigma) was used to lyse the cells. Immunoprecipitation was performed

(5) The dual action of glioma-derived exosomes on neuronal activity: synchronization and disruption of synchronyCell death & diseaseAugust 13, 2022Spelat R, Jihua N, Sánchez Triviño CA, Pifferi S, Pozzi D, Manzati M, Mortal S, Schiavo I, Spada F, Zanchetta ME, et al.were resuspended in CHAPS Cell Extract Buffer (Cell Signaling Technology, Danvers, MA, USA cat. no. 9852S) supplemented with protease inhibitor cocktail (Thermo Fisher Scientific, cat. no. 78430). 4X Laemmli

(6) The hexokinase “HKDC1” interaction with the mitochondria is essential for liver cancer progressionCell Death & DiseaseJuly 28, 2022Md. Wasim Khan, Alexander R. Terry, Medha Priyadarshini, Vladimir Ilievski, Zeenat Farooq, Grace Guzman, Jose Cordoba-Chacon, Issam Ben-Sahra, Barton Wicksteed, Brian T. Laydenflasks, when cells were 80% confluent, they were lysed using the CHAPS-lysis buffer (Cell Signalling #9852). Protein was collected by centrifuging the cell lysates at 12,000 x g. Protein homogenates were then

(7) A selective and rapid cell-permeable inhibitor of human caspase-3ACS Chemical BiologyNovember 15, 2019Angelo Solania, Gonzalo E. González-Páez, Dennis W. Wolaninduced. At the indicated time point, cells were harvested, resuspended in CHAPS cell extract buffer (CST 9852), lysed by freeze-thaw (3 cycles), clarified (10 min, 21,000 x g), and analyzed by Western blot. Western blot analysis

(8) Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor NeuronsFrontiers in neuroscienceJanuary 1, 2018Seminary ER, Sison SL, Ebert AD.2. Western blot Protein was isolated by resuspending cell pellets in 1x CHAPS Buffer (Cell Signaling, cat 9852S) followed by three freeze-thaw cycles. The protein concentration of the resulting lysate was determined

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Chaps Cell Extract Buffer (10X) from Cell Signaling Technology

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Chaps Cell Extract Buffer (10X) from Cell Signaling Technology

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